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SF/HGF-c-Met autocrine and paracrine promote metastasis of hepatocellular carcinoma

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World Journal of Gastroenterology 2001年第6期7卷 816-820页

作者： Qian Xie Kang-Da Liu Mei-Yu Hu Kang Zhou Experimental Research Center of zhongshan hospital,Fudan University,shanghai,20003.,china Experimental Research Center of zhongshan hospital Fudan University Shanghai 200032 China.

AIM: To explore the role of SF/HGF-Met autocrine and paracrine in metastasis of hepatocellular carcinoma (HCC). METHODS: SF/HGF and c-met transcription and protein expression in HCC were examined by RT-PCR and Western Blot in 4.HCC cell lines, including HepG2, Hep3B, SMMC7721 and MHCC-1, the last cell line had a higher potential of metastasis. sf/hgf cDNA was transfected by the method of Lipofectin into SMMC7721. SF/HGF and c-met antibody were used to stimulate and block SF/HGF-c-met signal transduction. Cell morphology, mobility, and proliferation were respectively compared by microscopic observation, wound healing assay and cell growth curve. RESULTS: HCC malignancy appeared to be relative to its met-SF/HGF expression. In MHCC-1, c-met expression was much stronger than that in other cell lines with lower potential of metastasis and only SF/HGF autocrine existed in MHCC-1. After sf/hgf cDNA transfection or conditioned medium of MHCC-1 stimulation, SMMC7721 changed into elongated morphology, and the abilities of proliferation (P < 0.05) and mobility increased. Such bio-activity could be blocked by c-met antibody (P < 0.05). CONCLUSION: The system of SF/HGF-c-met autocrine and paracrine played an important role in development and metastasis potential of HCC. Inhibition of SF/HGF-c-met signal transduction system may reduce the growth and metastasis of HCC.

关键词： Autocrine Communication Carcinoma, Hepatocellular Hepatocyte Growth Factor Humans Liver Neoplasms Paracrine Communication Proto-Oncogene Proteins c-met Research Support, Non-U.S. Gov't Tumor Cells, Cultured

Tedenan保护兔膀胱出口部分梗阻后逼尿肌细胞的超微结构研究

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复旦学报（医学版） 2003年第2期30卷 143-146页

作者：王国民王杭钟慈声复旦大学附属中山医院泌尿外科上海200032 上海医学院电子显微镜室上海200032

目的　探讨Tedenan(太得恩 )对兔膀胱出口部分梗阻后逼尿肌细胞超微结构的保护作用。方法　新西兰雄性白兔 2 0只 ,分 4. ,每组 5只。建立兔膀胱出口部分梗阻动物模型后两周 ,太得恩治疗组给予太得恩每天 10 0mg/kg喂服 ,花生油组给予... 详细信息

目的　探讨Tedenan(太得恩 )对兔膀胱出口部分梗阻后逼尿肌细胞超微结构的保护作用。方法　新西兰雄性白兔 2 0只 ,分 4. ,每组 5只。建立兔膀胱出口部分梗阻动物模型后两周 ,太得恩治疗组给予太得恩每天 10 0mg/kg喂服 ,花生油组给予花生油 (太得恩赋形剂 ) ,梗阻组不给任何药物。饲养 3周后解剖膀胱 ,测定膀胱容量及重量 ,应用透射电镜分别放大 6× 10 3 倍观察内质网 ,放大 2× 10 4.观察线粒体。结果　太得恩对兔膀胱出口部分梗阻所致的膀胱壁增生没有明显地抑制作用 ,而对膀胱容量缩小有明显的保护作用。太得恩组平均每个视野粗面内质网面积为 (1.2 5 9± 0 .2 74.) μm2 ,对照组为 (0 .2 14. 0 .10 1) μm2 ,梗阻组为 (4..75 0± 2 .15 2 ) μm2 ,花生油组为 (5 .76 1± 1.775 ) μm2 。单位视野内太得恩组内质网面积明显小于梗阻组及花生油组 (P 4.,对照组为 1.5 82± 0 .2 86 ,梗阻组为0 .973± 0 .0 73,花生油组为 0 .92 5± 0 .10 2。对照组和太得恩组相对密度比较无明显差异 ,而明显高于梗阻组和花生油组 (P <0 .0 5 )。

关键词：膀胱出口梗阻逼尿肌细胞超微结构太得恩药物治疗

Association of apolipoprotein E 4.polymorphism with cerebral infarction in Chinese Han population

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Acta Pharmacologica Sinica 2004年第03期25卷 98-102页

作者： Zhu-qing JIN2,Yong-sheng FAN3,Jing DING4.Mei CHEN5,Wei FAN4.Guang-ji ZHANG2,Bin-hui ZHANG5,Suo-jing YU2,Yong-sheng ZHANG2,Wei-feng JI5,Jian-gang ZHANG6 2Genomic Research Centre,Zhejiang College of Traditional Chinese Medicine,Hangzhou 310053 3Schoolof Basic Medical Sciences,Zhejiang College of Traditional Chinese Medicine,Hangzhou 310053 4.epartment of Neurology,zhongshan hospital,Fudan University,shanghai20003. 5Department of Neurology,Zhejiang hospital of Traditional Chinese Medicine,Zhejiang Collegeof Traditional Chinese Medicine,Hangzhou 310006,china Genomic Research Centre Zhejiang College of Traditional Chinese Medicine Hangzhou 310053 School of Basic Medical Sciences Zhejiang College of Traditional Chinese Medicine Hangzhou 310053 Department of Neurology Zhongshan Hospital Fudan University

AIM: To study the association between APOE polymorphisms and cerebral infarction through a case-control study among the Chinese Han population. METHODS: First-ever cerebral infarction patients (n=226) whose ages ranged from 4. to 60 years old were recruited from Department of Neurology, zhongshan hospital, shanghai, and Zhejiang Chinese Traditional Medicine hospital, Zhejiang, china. Unrelated healthy controls (n=201) were selected from the general population in the same area with similar age and sex distribution. APOE was amplified by one-stage PCR using the forward primer: 5'-GGC ACG GCT GTC CAA GGA GCT-3' and reverse primer: 5'-GAT GGC GCTGAG GCC GCG CT-3'. The PCR product was digested directly with 5 U of CfoI and separated by a 20 % polyacryla- mide (acrylamide: bis-acrylamide=29:1) nondenaturing gel. RESULTS: Both cerebral infarction patient and control groups were in Hardy-Weinberg equilibrium. The allele frequency of APOE*2, APOE*3, and APOE*4.was 4.6 %, 81.9 %, and 13.5 % respectively in the patients with cerebral infarction; 5.7 %, 87.3 %, and 7.0 % respectively in the healthy control group. Compared with APOE3/3 subjects, APOE4.4.carriers had a 2.1-fold risk of cerebral infarction (odds ratio 2.1, 95 % confidence limits 1.3 to 3.4.. The allele frequency of APOE*4.in the cerebral infarction patient group was significantly higher than that in the control group (13.5 % vs 7.0 %; P=0.002). CONCLUSION: APOE 4.is a risk factor for cerebral infarction among the Chinese Han population.

关键词： apolipoproteins E human alleles polymorphism brain infarction

补肾泻肝方对辐射诱导小鼠骨髓有核细胞凋亡及基因表达影响

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复旦学报（医学版） 2002年第6期29卷 486-488,397页

作者：史剑慧许小平程文英牛玉宏葛均波复旦大学附属中山医院-上海市心血管病研究所上海200032 第二军医大学长海医院血液科上海200433 复旦大学放射医学研究所上海200032

目的　探索补肾泻肝方 (“BuShenXieGanFang” ,简作补泻方 ,“BXF”)减少辐射所致小鼠骨髓有核细胞(BMNCs)凋亡的作用机制。方法　用TdT介导的脱氧核苷酸切口和末端标记法 (Tunel)检测补泻方对辐射诱导的小鼠BMNCs凋亡的影响 ;应用RT ... 详细信息

目的　探索补肾泻肝方 (“BuShenXieGanFang” ,简作补泻方 ,“BXF”)减少辐射所致小鼠骨髓有核细胞(BMNCs)凋亡的作用机制。方法　用TdT介导的脱氧核苷酸切口和末端标记法 (Tunel)检测补泻方对辐射诱导的小鼠BMNCs凋亡的影响 ;应用RT PCR方法检测补泻方对辐射诱导的小鼠BMNCsIL 1和wtp5 3基因表达水平的调控作用。结果　补泻方能明显减少 4.y照射后 2～ 2 4.的BMNCs凋亡。在 2～ 10Gy剂量照射后 6h ,补泻方能减少BMNCs凋亡 ,尤以 2、4.8、10Gy为显著。辐射诱导IL 1和wtp5 3基因表达 ,基因表达随辐射剂量增加而增加。用药组骨髓细胞经 2、4.6和 8Gy照射后IL 1基因表达显著高于对照组 (P 4.6和 8Gy照射后wtp5 3基因表达显著低于对照组 (P <0 .0 5 )。显示补泻方能增加辐射所诱导的内源性辐射防护剂IL 1基因的表达 ;降低辐射所诱导的与造血细胞凋亡有关的凋亡基因wtp5 3的表达。结论　补泻方显著改善辐射所致骨髓细胞凋亡的机制与调控辐射所诱导的与造血细胞凋亡有关的IL 1和wtp5 3基因的表达有关。

关键词：补肾泻肝方辐射小鼠骨髓有核细胞细胞凋亡基因表达

Dynamic expression of proteins associated with adventitial remodeling in adventitial fibroblasts from spontaneously hypertensive rats

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Dynamic expression of proteins associated with adventitial r...

第十三次全国心血管病学术会议

作者： GUO Shu-jie,LI Yan,GAO Ping-jin,Zhu Ding-liang Ruijin hospital,shanghai Jiaotong University School of Medicine, shanghai institute of {4. Diseases,shanghai 200025, china

Objective To identify proteins that could potentially be involved in adventitial remodeling in vascular adventitial fibroblasts(AFs) from spontaneously hypertensive rats(SHR).Methods AFs were isolated from thoracic aorta of 4.8, 16,and 24.week-old male SHR and Wistar-Kyoto(WKY) and cultured to passage *** differential expression profile between SHR-AF and WKY-AF were investigated using 2-D electrophoresis(2-DE),whereas gel image analysis was processed using Image Master 2D *** spot was identified by Matrix-assisted laser desorption/ionization time-

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再论中枢神经兴奋药

新药与临床 1989年第1期8卷 58-61页

作者：吴珏上海医科大学附属中山医院上海200032

高效合理使用中枢神经兴奋药的基本观点,近年来有明显的变革。目前,临床上呼吸与循环的抢救与功能的维持,已不再依靠中枢神经兴奋药;昏迷重症的催醒,也不依靠本类药物。另一方面,本类药尤其是苯丙胺和哌醋甲酯等,对小儿的多动贪玩、注... 详细信息

高效合理使用中枢神经兴奋药的基本观点,近年来有明显的变革。目前,临床上呼吸与循环的抢救与功能的维持,已不再依靠中枢神经兴奋药;昏迷重症的催醒,也不依靠本类药物。另一方面,本类药尤其是苯丙胺和哌醋甲酯等,对小儿的多动贪玩、注意力不集中,常能有所帮助。使用较广的多沙普仑能兴奋呼吸,时效仅10min许,疗效有限。其他过去常用的一些中枢神经兴奋药,现均已无实用价值。

关键词：中枢兴奋药咖啡因哌醋甲酯

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心钠素在心肌病中浓度变化的意义

上海医学 1992年第4期15卷 201-204页

作者：朱文青林佑善陈灏珠上海医科大学附属中山医院上海市心血管病研究所

本文研究了19例心肌病的血浆心钠素浓度变化,并与风心病及正常人进行了比较。结果显示扩张性心肌病心功能不全组显著高于对照组(P<0.01),且与左房内径和左室舒张末期内径成正相关。作者认为在病程中测定血浆心钠素水平可以作为判断心肌... 详细信息

本文研究了19例心肌病的血浆心钠素浓度变化,并与风心病及正常人进行了比较。结果显示扩张性心肌病心功能不全组显著高于对照组(P<0.01),且与左房内径和左室舒张末期内径成正相关。作者认为在病程中测定血浆心钠素水平可以作为判断心肌损害程度及病情进展,以及作为估价扩张性心肌病发生心功能不全时的一种非创伤性参数。

关键词：心钠素扩张性心肌病左房内径

The pre-clinical assessment of a novel biodegrable rapid prohealing polymer with sirolimus-eluting coating stent in porcine model

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The pre-clinical assessment of a novel biodegrable rapid pro...

第十三次全国心血管病学术会议

作者： SHEN LUGE Jun-bo,ZHONG Wei,YANG Wei,WU Yi-zhe, TIAN Wen-jie,SUN Ai-jun,ZOU Yun-zen shanghai institute of cardiovascular Diseases,zhongshan {4., Fudan University,shanghai 20003.,china

Objective To develop rapid healing of endothelium on the stent surface by pavement of a cytophilic biodegradable natural polymer as well as anti-proliferative drug(sirolimus).Methods A layer-by-layer(LBL) self-assembly coating of chitosan /heparin(CS/HEP) onto a luminal side of coronary stent was designed for the acceleration of the re-endothelialization and sirolimus was only coated onto vessel wall side of stent to suppressed neointimal *** biocompatibility and

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食管鳞癌中肿瘤微血管密度的临床意义

复旦学报（医学版） 2004年第2期31卷 162-164,168,F005页

作者：范虹曾亮王群复旦大学附属中山医院胸外科上海200032

目的　研究分析食管癌组织中肿瘤微血管密度 (MVD)的临床意义。方法　采用免疫组化的方法检测4.5例食管鳞癌根治性切除术后的肿瘤组织中的肿瘤微血管密度 ,应用SPSS统计软件分析肿瘤MVD与食管鳞癌临床病理参数及预后的关系。结果　本组... 详细信息

目的　研究分析食管癌组织中肿瘤微血管密度 (MVD)的临床意义。方法　采用免疫组化的方法检测4.5例食管鳞癌根治性切除术后的肿瘤组织中的肿瘤微血管密度 ,应用SPSS统计软件分析肿瘤MVD与食管鳞癌临床病理参数及预后的关系。结果　本组病例中 ,肿瘤MVD平均值为 15 .5 8,中位值为 15。MVD水平与食管癌的肿瘤局部浸润程度、区域淋巴结转移及TNM分期有关 (P 15 )病例的术后生存率低于少血管型 (MVD≤ 15 )病例 (P =0 .0 2 78)。Cox比例风险模型证实MVD水平是食管癌根治性切除病人预后的独立预测指标。结论　食管鳞癌的血管新生与癌肿进展程度有关 ,高MVD的食管鳞癌患者术后生存率明显低于低MVD的患者。

关键词：食管鳞癌肿瘤微血管密度预后