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A floral organ moving like a caterpillar for pollinating

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Journal of Systematics and Evolution 2010年第2期48卷 102-108页

作者： 1,3,4.hong-Jian LIU 1Li-Jun CHEN 1,2.3Ke-Wei LIU 1Li-Qiang LI 1Wen-Hui RAO 1(The National Orchid Conservation Center of China/The Orchid Conservation & Research Center of Shenzhen,Shenzhen 518114.China) 2.{2. of Life Sciences,university of Science & Technology of China,Hefei 2.002.,China) 3(The Center for Biotechnology and BioMedicine,Graduate {2. at Shenzhen,Tsinghua university,Shenzhen 518055,China) 4.College of {4.,South China Agricultural university,Guangzhou 51064.,China) (The National Orchid Conservation Center of China/The Orchid Conservation & Research Center of Shenzhen Shenzhen 518114 China) (school of Life Sciences University of Science & Technology of China Hefei 230027 China) (The Center for Biotechnology and BioMedicine Graduate School at Shenzhen Tsinghua University Shenzhen 518055 China) (College of forestry South China Agricultural University Guangzhou 510642 China) *Correspondence: Zhong-Jian LIU *Correspondence: *?Author for correspondence. E-mail: <liuzj@***>.

A new pollination mechanism is reported of an orchid species,Bulbophyllum penicillium,based on a field observation in Southeast Yunnan of *** species has a sensitive lip,and there is a distance of 2.3 mm between it and column *** the lip is touched by a landing insect,it will move up and down or swing left and right continuously,just like a moving *** suck a lip movement,the pollinator,a very small fruit fly (Drosophila sp.) ca.1 mm in height,will be pressed toward the column apex where anther and stigma are located,and then cross-pollination takes *** unique mode of pollination,depending on the movement of lip rather than insect itself,has never been found before in either Orchidaceae or other families of angiosperms.

关键词： pollination mechanism Bulbophyllum penicillium Drosophila lip movement

A new orchid Paphiopedilum guangdongense and its molecular evidence

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Journal of Systematics and Evolution 2010年第5期48卷 350-355页

作者： 1 Li-Jun CHEN 1,2.3Zhong-Jian LIU 4.ou-Yu LI 1Li-Qiang LI 1(Shenzhen Key Laboratory for Orchid Conservation and Utilization,The National Orchid Conservation Center of China/The Orchid Conservation and Research Center of Shenzhen,Shenzhen 518114.China) 2.The Center for Biotechnology and BioMedicine,Graduate {2. at Shenzhen,Tsinghua university,Shenzhen 518055,China) 3(College of forestry,South China Agricultural university,Guangzhou 51064.,China) 4.The Nature Reserve of Dawuling of Guangdong Province,Xinyi 52.000,Guangdong,China) (Shenzhen Key Laboratory for Orchid Conservation and Utilization The National Orchid Conservation Center of China/The Orchid Conservation and Research Center of Shenzhen Shenzhen 518114 China) (The Center for Biotechnology and BioMedicine Graduate School at Shenzhen Tsinghua University Shenzhen 518055 China) (College of forestry South China Agricultural University Guangzhou 510642 China) (The Nature Reserve of Dawuling of Guangdong Province Xinyi 525000 Guangdong China) *Correspondence: Zhong-Jian LIU *Correspondence: ?Author for correspondence. E-mail: liuzj@*** Tel.: 86-755-2.712.59 Fax: 86-755-2.71192..

A new Paphiopedilum entity found in southern Guangdong was studied taxonomically by means of internal transcribed spacer of nuclear ribosomal DNA analysis in addition to morphological and phytogeographical *** entity is similar to Paphiopedilum gratrixianum(Masters)Rolfe morphologically,from which it differs by having narrower leaves not wider than 2.cm and lacking purple spots toward abaxial base,pale green dorsal sepal veined with pale purplish brown and not heavily spotted with purple,and staminode subtruncate and mucronate at *** analysis of internal transcribed spacer sequences of this entity and its allies came to the same conclusion that the entity is a member of Paphiopedilum section Paphiopedilum and closely related to but distinct from *** a result,it is treated here as a new species,Paphiopedilum guangdongense ***&***.

关键词： new species nrITS Orchidaceae Paphiopedilum guangdongense.

THE INVOLVEMENT OF CDC4. IN MORPHOGENESIS AND PATHOGENESIS OF MAGNAPORTHE GRISEA

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THE INVOLVEMENT OF CDC42 IN MORPHOGENESIS AND PATHOGENESIS O...

The ⅩⅤth International Plant Protection Congress

作者： ZHENG Wu1 LU Guo-dong1 KE Hai-yan2.LIU Wen-de1 ZHAO Zhi-ying1 3 WU Sheng-cheng3 YANG Zhen-biao4.and WANG Zong-hua2.1 1College of Plant Protection Fujian Agriculture and forestry {2. Fuzhou China 350002.2.3} of Life science Fujian Agriculture and forestry {2. 3Complex Carbohydrate Research Center {2. of Georgia usa 4.ept. Bot. and Plant Sci {2. of California Riverside CA 92.2.-012.

Changes in Phosphorus Fractions, pH, and Phosphatase Activity in Rhizosphere of Two Rice Genotypes

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Pedosphere 2008年第6期18卷 785-794页

作者： LI Yong-Fu LUO An-Cheng WEI Xing-Hua YAO Xu-Guo College of Environmental and Resource Science Zhejiang University Hangzhou 310029 （China） school of Environmental Science and Technology Zhejiang Forestry University Lin＇an 311300 （China） State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 （China）

A rhizobox experiment with two phosphorus (P) treatments, zero-P (0 mg P kg-1) and plus-P (100 mg P kg-1) as Ca(H2.O4.2.H2., was conducted to study the chemical and biochemical properties in the rhizosphere of two rice genotypes (cv. Zhongbu 51 and Pembe) different in P uptake ability and their relationship with the depletion of soil P fractions. Plant P uptake, pH, phosphatase activity, and soil P fractions in the rhizosphere were measured. Both total dry weight and total P uptake of Pembe were significantly (P al-P were not depleted in the rhizosphere of both genotypes, regardless of P treatments despite significant acidification in the rhizosphere of Pembe under zero-P treatment. Higher acid phosphatase (AcPME) activity and alkaline phosphatase (alPME) activity were observed in the rhizosphere of both Zhongbu 51 and Pembe compared to the corresponding controls without plant. AcPME activity was negatively (P alization of soil organic P.

关键词： phosphatase phosphorus fractions rhizosphere rice genotypes soil pH

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Studies on Neural Network Modeling for Air Conditioning System by Using Data Mining with Association Analysis

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Studies on Neural Network Modeling for Air Conditioning Syst...

2.10 4.h International Conference on Intelligent Information Technology Application (IITA2.10)

作者： Zheng Wang school of Technology, Beijing forestry {2., Beijing, China Ning Han school of Technology, Beijing forestry {2., Beijing, China Yang Wang Beijing Master Systems Engineering Co.,Ltd Beijing, China

VAV air conditioning system involves multiple control parameters and they have strong coupling with each other, which caused the modeling complex. A neural network model of air handling unit （AHU） is constructed through data preprocessing and association analysis as mean of data mining is made to identity interesting information between the supply air temperature and the other variables acquired by air conditioning system. The study provides new idea and technical support for AHU modeling of VAV air conditioning system.

关键词： VAV air conditioning system data mining association analysis neural network modeling

Phosphoproteomic identification and phylogenetic analysis of ribosomal P-proteins in Populus dormant terminal buds

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Phosphoproteomic identification and phylogenetic analysis of...

第二届中国林业学术大会——S2.功能基因组时代的林木遗传与改良

作者： Chang-Cai Liu1)a Tian-Cong Lu 1)a Hua-Hua Li3) Gui-Feng Liu5) Bai-Chen Wang8)b (Education Ministry Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Northeast forestry university Harbin 1500{2.)Hong-Xia Wang{2. (Institute of Basic Medical Sciences, National Center for Biomedical Analysis Beijing 100850) Ling Ma6) (forestry school, Northeast forestry university Harbin 1500{2.) Chuan-Ping Yang7)b (Education Ministry Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Northeast forestry university Harbin 1500{2. forestry school, Northeast forestry university Harbin 1500{2.)

To better understand the role that reversible phosphorylation plays in woody plant ribosomal P-protein function, we initiated a phosphoproteomic investigation of P-proteins from Populus dormant terminal buds. Using gel-free (in-solution) protein digestion and phosphopeptide enrichment combined with a nanoUPLC-ESI-MS/MS strategy, we identified six phosphorylation sites on eight P-proteins from Populus dormant terminal buds. Among these, six Ser sites and one Thr site were identified in the highly conserved C-terminal region of eight P-proteins of various P-protein subfamilies, including two P0, two P1, three P2. and one P3 protein. Among these, the Thr site was shown to be novel and has not been identified in any other organisms. Sequence analysis indicated that the phosphothreonine sites identified in the C-terminus of Ptr RPP2. exclusively occurred in woody species of Populus etc. The identified phosphopeptides shared a common phosphorylation motif of (S/T)XX(D/E) and may be phosphorylated in vivo by casein kinase 2.as suggested by using Scansite analysis. Furthermore, phylogenetic analysis suggested that divergence of P2.also occurred in Populus, including Type I and Type II. To the best of our knowledge, this is the first systematic phosphoproteomic and phylogenetic analysis of P-proteins in woody plants, the results of which will provide a wealth of resources for future understanding and unraveling of the regulatory mechanisms of Populus P-protein phosphorylation during the maintenance of dormancy.

关键词： Dormant terminal buds NanoUPLC-ESI-MS/MS Phosphoproteomics Phylogenetic analysis P-proteins

forestry Development and Environmental Protection in China Since 1980

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Forest Ecosystems 2003年第S1期5卷 1-4页

作者： Liu Junchang~* Song Weiming school of Economics and Management,Beijing forestry {2.,Beijing 100083,P.R.China

Forest,as the main body of the terrestrial ecological system and the important natural resource on which themankind relies for subsistence,not only can provide timber and other forest products,but also has the function of adjusting climate,conserving water and soil,wind-breaking and fixing sand,*** plays an irreplaceable role in maintaining the natural *** 1978,the opening and reform policy has been carried out in China,the Chinese economy has been developingvery *** the same time,Chinese government has given priority to tree planting and forestry development which are seen assignificant contributions to the development of the ecological *** upsurge of tree planting and forest protecting hasbeen set off one after another by organizing and mobilizing the people all over *** work has been done to developforestry and to improve the landscape of the *** this paper,the development of forestry in the last 2. years has been reviewedand major forestry policies have been *** on the development of national economy and forestry situation,the 4.majorstrategic measures have been pointed out for the development of forestry in the future.

关键词： forestry development ecological system forestry industry system forestry policy

Identification, characteristic and phylogenetic analysis of type II DNA topoisomerase gene in Giardia lamblia

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Cell Research 2005年第6期15卷 474-482页

作者： De HE1, 2. 3, Jian Fan WEN1,*, Wan Qun CHEN1, Si Qi LU4. De Dong XIN1, 2. Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 6502.3, China2.Gradulate school of the Chinese Academy of Sciences, Beijing 100039, China3 Southwest {2. College, Kunming 6502.4. China4.Capital university of Medical Sciences, Beijing 100054. China KeyLaboratoryofCellularandMolecularEvolution KunmingInstituteofZoologyChineseAcademyofSciencesKunming650223China

The genes encoding type II DNA topoisomerases were investigated in Giardia lamblia genome, and a type IIA gene,GlTop 2.was identified. It is a single copy gene with a 4.76 bp long ORF without intron. The deduced amino acidsequence shows strong homology to eukaryotic DNA Top 2. However, some distortions were found, such as sixinsertions in the ATPase domain and the central domain, a ~100 aa longer central domain; a ~2.0 aa shorter C-terminaldomain containing rich charged residues. These features revealed by comparing with Top 2.of the host, human, mightbe helpful in exploiting drug selectivity for antigiardial therapy. Phylogenetic analysis of eukaryotic enzymes showedthat kinetoplastids, plants, fungi, and animals were monophyletic groups, and the animal and fungi lineages shared amore recent common ancestor than either did with the plant lineage; microsporidia grouped with fungi. However, unlikemany previous phylogenetic analyses, the “amitochondriate” G. lamblia was not the earliest branch but diverged aftermitochondriate kinetoplastids in our trees. Both the finding of typical eukaryotic type IIA topoisomerase and the phylo-genetic analysis suggest G. lamblia is not possibly as primitive as was regarded before and might diverge after theacquisition of mitochondria. This is consistent with the recent discovery of mitochondrial remnant organelles in G. lamblia.

关键词： evolutionary position GlTop2.gene Giardia lamblia identification phylogenetic analysis type II DNAtopoisomerase.

Studies on Diagnostic Model of Breast Cancer Based on Saliva Proteome and Bioinformatic

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Studies on Diagnostic Model of Breast Cancer Based on Saliva...

2.10 International Conference on Future Information Technology and Computing (FITC 2.10)

作者： Cao Meiqun1,2.Wu Weikang2.Gui Zifan3,Sun Kehuan1,Wu Zhengzhi1,4.1.Shenzhen Institute of Integrated Traditional Chinese and Western Medicine,Shenzhen Second People’s Hospital,Shenzhen,China 2.Zhongshan school of Medicine,Sun Yat-Sen {2.,Guangzhou,China 3.College of science and technology,Central South {2. of forestry and Technology,Changsha,China 4.Shenzhen Institute of geriatric medicine Shenzhen,China.

Aim:This study was designed to construct a salivary protein fingerprint model used for early diagnosis of breast cancer by detecting salivary proteins of breast cancer patients and screening specific protein ***:Surface-enhanced laser desorption ionization time-of-flight mass spectrometry（SELDI-TOF-MS） technique was applied to determine the protein mass spectrums of 91 cases of saliva samples（among which 4. cases were breast cancer patients and 4. cases were healthy subjects） to establish a diagnosis model of breast ***:311 protein peaks were detected in the samples of breast cancer patients and healthy control,and 32.protein peaks had statistical significance difference between the two groups.A template consisting of 4.proteins with molecular weights of 4.4..31,52.4.96,34.9.02.and 3559.89 Da respectively was *** could be correctly grouped to breast cancer patients and healthy *** 4. cases of breast cancer,37 cases were correctly diagnosed;34.cases of healthy subjects were correctly *** sensitivity was 78.7%（37/4.）;the specificity was 77.2.（34.4.）.Conclusions:the salivary protein fingerprint model of breast cancer constructed by SELDI-TOF-MS technique provided a new method with strong specificity and high sensitivity for early screening and diagnosis of breast cancer;and it was worth further research and application.

关键词： SELDI-TOF-MS Diagnosis Model Bioinformatics Breast Cancer Proteome Salivia