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Structural parameterization and functional prediction of antigenic polypeptome sequences with biological activity through quantitative sequence-activity models (QSAM) by molecular electronegativity edge-distance vector (VMED)

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Science china(Life Sciences) 2007年第5期50卷 706-716页

作者： LI ZhiLiang1,2. WU ShiRong1,2. CHEN ZeCong1,2. YE Nancy1,2. YANG ShengXi1,2. LIAO ChunYang1,2. ZHANG MengJun1,2.3, YANG Li1,2. MEI Hu1,2.4. YANG Yan1,2. ZHAO Na1,2. ZHOU Yuan1,2. ZHOU Ping1,2. XIONG Qing1,2. XU Hong1,2. LIU ShuShen1,2. LING ZiHua1,2. CHEN Gang1,2.4.& LI GenRong1,2.1 College of Chemistry and Chemical {2./Key Laboratory for Chemobiomedical Science and {2. under Chongqing Municipality, College of Life Science and biological {2./Key Laboratory for biomechanics and Tissue {2. under Ministry of Education, Chongqing University, Chongqing 4.004., china 2.State Key Laboratory for Chemobiosensors and Chemobiometrics under MOST at hunan University, changsha 4.0012. china 3 Department of Medical Analysis/PLA center of bioinformatics Immunology, Surgeon Third University, Chongqing 4.0031, china 4.Technology Centre for Life Sciences, Singapore Polytechnic, 500 Dover Road, Singapore 139651, Singapore 1. College of Chemistry and Chemical engineering/Key Laboratory for Chemobiomedical Science and engineering under Chongqing Municipality College of Life Science and Biological Engineering/Key Laboratory for Biomechanics and Tissue Engineering under Ministry of Education Chongqing University Chongqing 400044 China 2. State Key Laboratory for Chemobiosensors and Chemobiometrics under MOST at {2. University Changsha 410012 China 3. Department of Medical Analysis/PLA center of bioinformatics Immunology Surgeon Third University Chongqing 400031 China 4. Technology Centre for Life Sciences Singapore Polytechnic 500 Dover Road Singapore 139651 Singapore

Only from the primary structures of peptides, a new set of descriptors called the molecular electro-negativity edge-distance vector (VMED) was proposed and applied to describing and characterizing the molecular structures of oligopeptides and polypeptides, based on the electronegativity of each atom or electronic charge index (ECI) of atomic clusters and the bonding distance between atom-pairs. Here, the molecular structures of antigenic polypeptides were well expressed in order to propose the auto-mated technique for the computerized identification of helper T lymphocyte (Th) epitopes. Furthermore, a modified MED vector was proposed from the primary structures of polypeptides, based on the ECI and the relative bonding distance of the fundamental skeleton groups. The side-chains of each amino acid were here treated as a pseudo-atom. The developed VMED was easy to calculate and able to work. Some quantitative model was established for 2. immunogenic or antigenic polypeptides (AGPP) with 14.(1― 14. Ad and 14.other restricted activities assigned as "1"(+) and "0"(-), respectively. The latter comprised 6 Ab(15-2.), 3 Ak(2.-2.), 2.Ek(2.0}2.), 2.H-2.(2. and 2.) restricted sequences. Good results were obtained with 90% correct classification (only 2.wrong ones for 2. training samples) and 100% correct prediction(none wrong for 8 testing samples); while con-trastively 100% correct classification (none wrong for 2. training samples) and 88% correct classification (1 wrong for 8 testing samples). Both stochastic samplings and cross valida-tions were performed to demonstrate good performance. The described method may also be suitable for estimation and prediction of classes I and II for major histocompatibility an-tigen (MHC) epitope of human. It will be useful in immune identification and recognition of pro-teins and genes and in the design and devel-opment of subunit vaccines. Several quantitative structure activity relationship (QSAR) models were developed for various oligop

关键词： molecular electronegativity distance-edge vector (VMED), antigenic polypeptide (AGPP) sequences, bioactive oligopeptide (BAOP) chains, quantitative sequence-activity models (QSAM), theoretically computational descriptors (TCD)

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元宝草化学成分的研究

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天然产物研究与开发 2002年第5期14卷 50-53页

作者：曾虹燕周朴华裴刚湘潭大学化工学院湖南农业大学理学院湖南中医学院湘潭4.1105 长沙4.012. 长沙4.0007

目的　研究中药元宝草 (HypericumsampsoniiH .)的化学成分。方法　用乙醇或甲醇回流提取 ,聚酰胺柱色谱和重结晶分离 ,FT -IR ,MS ,UV ,1HNMR等方法确定结构。结果　分得了 4.化学成分 ,分别为芦丁 ,金丝桃苷 ,槲皮素和金丝桃素。结... 详细信息

目的　研究中药元宝草 (HypericumsampsoniiH .)的化学成分。方法　用乙醇或甲醇回流提取 ,聚酰胺柱色谱和重结晶分离 ,FT -IR ,MS ,UV ,1HNMR等方法确定结构。结果　分得了 4.化学成分 ,分别为芦丁 ,金丝桃苷 ,槲皮素和金丝桃素。结论　 4.化合物均为首次从元宝草中分得和检出。

关键词：元宝草金丝桃素芦丁金丝桃苷槲皮素

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Surveillance of Schistosoma japonicum Infection in Domestic Ruminants in the Dongting Lake region,hunan Province,china

Surveillance of Schistosoma japonicum Infection in Domestic ...

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中国畜牧兽医学会兽医公共卫生学分会第三次学术研讨会

作者： Jinming Liu1,Chunxia Zhu2.Yaojun Shi1,Hao Li1,Lanpin Wang2.Shangtian Qin2.Saie Kang3,Yanpin Huang4.Yamei Jin1 & Jiaojiao Lin 1(1.National Laboratory of Animal Schistosomiasis Control/Key Laboratory of Animal Parasitology,Ministry of Agriculture,Shanghai Veterinary research Institute,Chinese Academy of Agricultural Sciences,Shanghai,PR {2. 2.hunan Animal Disease Control center,{4.,PR {2. 3.Yueyang County Animal Husbandry Bureau,Yueyang,PR {2. 4.Yuanjiang Municipal Animal Husbandry and Fishery Bureau,Yuanjiang,PR {2.)

Background:Schistosomiasis japonica is prevalent in Asian countries and it remains a major public health problem in *** major endemic foci are the marsh and lake regions of southern china,particularly the Dongting Lake region bordering hunan and Hubei provinces,and the Poyang Lake region in Jiangxi *** ruminants,especially bovines,have long been considered to play a major role in the transmission of Schistosoma japonicum to *** and Findings:A miracidial hatching technique was used to investigate the prevalence of *** infections in domestic ruminants and field feces collected from two towns located to the south and east of Dongting Lake,hunan province,between 2.05 and *** overall prevalence of infection was not significantly reduced from 4.93% in 2.05 to 3.64. in 2.08,after which it was maintained at this *** comprised 2..5-58.2. of the total infected ruminants,while goats comprised 4..8-76.5%.Infection rates in cattle and goats were significantly higher than those found in buffalo in most study *** prevalence in buffalo younger than three years was significantly higher than that in those aged over three *** the positive field samples of feces were derived from bovines in *** Matang Town,61.2.% of the positive field feces were from bovines,while the rest were from *** positive rates for field feces were approximately the same in April and November/***:The present study found that bovines and goats are major sources of *** infection in the Dongting lake region and there was age-related resistance in *** bovines and goats should be treated equally when controlling *** infections in the Dongting lake *** is essential to conduct an additional mass treatment in late March or early April,in addition to the original treatment scheme.

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Studies on Heteroses of Pei'ai 64./Javanica Rice

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hunan Agricultural Science ＆ Technology Newsletter 2002年第3期3卷 4-10页

作者： Tang Li, Xiao Guoying, Yuan Longping, and Deng Xiaoxiangchina National Hybrid Rice research and Development {2., changsha 4.012., china china National Hybrid Rice research and Development {2. Changsha 410125 China

Agronomic traits and heteroses of javanica varieties and the indica/javanica hybrids in changsha are reported. Javanica rice exhibits long panicles, big grains, less panicles per plant, a long growth duration and high plant height in changsha. The hybrid of Pei'ai 6{2./javanica possesses positive heterobeltiosis in culm height, panicle length, panicles per plant, spikelets per panicle, grains per panicle, yield per plant, theoretic yield per hectare and actual yield per hectare; performs negative heterobeltiosis in days to flower, seed setting rate and 1000-grain weight. There are significant linear regression relations between culm height of the hybrid and panicle length of its male parent, panicle length of the hybrid and that of its male parent, spikelets per panicle of the hybrid and spikelets per panicle and panicle length of its male parent, grains per panicle of the hybrid and spikelets per panicle of its male parent, 1000-grain weight of the hybrid and spikelets per panicle of its male parent, respectively. In general, significant differences at 1% level are seen in most traits except grains per panicle and theoretic yield per hectare between the indica/javanica hybrid and Pei'ai 6{2./Teqin (CK). Compared with the check, the indica/javanica hybrids have positive Standard heterosis in culm height, panicle length, spikelets per panicle, grains per panicle and 1000-grain weight but negative standard heterosis in days to flower, panicles per plant, seed setting rate, theoretic yield per hectare and actual yield per hectare. For the individuals, 11.1% hybrids possess > {2.% standard heterosis in theoretic yield per hectare, 3.7% possess >{2.% standard heterosis in actual yield per hectare. The javanica restorer line 770 derives from a single cross of Pei'ai 6{2./Moroberekan B-51-{2.9-2. The hybrid {2.S/770 possesses an elite plant type and strong heterosis.

关键词： javanica rice Pei'ai 64. heterosis hybrid rice

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Thermal ablation versus conventional regional hyperthermia has greater anti-tumor activity against melanoma in mice by upregulating CD4.+ cells and enhancing IL-2.secretion

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Progress in Natural Science:Materials International 2009年第12期19卷 1699-1704页

作者： Yingying Zhang~(a,b),Wei Zhang~b,Cuanying Geng~c,Tongjun Lin~d,Xiaowen Wang~b, Lingyun Zhao~b,Jintian Tang~(b,*) a Department of Oncology,Xiangya Hospital,Central South University,changsha 4.0008,{2. b Institute of Medical Physics and engineering,Department of engineering Physics,Tsinghua University,Beijing 100084.{2. c Clinical Medical research center,{2.-Japan Friendship Hospital,Beijing 10002.,{2. d Department of Microbiology & Immunology,Department of Pediatrics,IWK Health Centre,Dalhousie University,5850 University Avenue, Halifax,NS,Canada B3J 3G9 [a]Department of Oncology Xiangya Hospital Central South University Changsha 410008 China [b]Institute of Medical Physics and engineering Department of Engineering Physics Tsinghua University Beijing 100084 China [c]Clinical Medical research center China–Japan Friendship Hospital Beijing 100029 China [d]Department of Microbiology & Immunology Department of Pediatrics IWK Health Centre Dalhousie University 5850 University Avenue Halifax NS Canada B3J 3G9

To determine whether conventional hyperthermia(4.-15℃) or ablation therapy(>50℃) achieves better synergistic effects on direct cytotoxicity and anti-tumor immunity,we compared the therapeutic effects of two hyperthermia temperatures,4. and 55℃,in terms of cytotoxicity and upregulation of immune functions in a mouse malignant melanoma ***-bearing mice were treated by directly applying regional hyperthermia to the tumor nodule with a heating light at a temperature of 4.℃for 30 min or 55℃for 10 *** tumor growth curve and mice survival rate were *** investigate the hyperthermia-induced immunological response,peripheral blood CD4.+ and CD8~+ T cells and the serum IL-2.level were *** results indicated that application of regional hyperthermia at the ablation temperature(such as 55℃) achieved better synergistic anti-tumor effects than did conventional hyperthermia(4.℃).Significant increases in the number of peripheral blood CD4.+ T cells and the serum IL-2.level likely contributed to the underlying mechanism.

关键词： Hyperthermia Thermal ablation Melanoma Immunity response

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research on Extracting Forest Stand Information based on 3D Laser Scanner Techniques

Research on Extracting Forest Stand Information based on 3D ...

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2.12.IEEE International Conference on Computer Science and Automation engineering（CSAE 2.12.

作者： LU Kangning 1 ZHANG Huaiqing 2.GAO Shizeng 3 JIANG Xian 4.research Institute of Forestry Resource Information Techniques Chinese Academy of Forestry Beijing {2. WU Xueming 1 ZHANG Ning 2.KUANG Yilong 3 research center of Forestry Remote Sensing & Information {4. Central South University of Forestry & Technology changsha Hu’nan {2.

The development of laser scanning technology provides a new way to make people obtain spatial information from the traditional manual to *** this study,we take the FARO Laser Scanner Photon as the data obtained platform,we scanned a 10 m×15 m plot,total 2. trees in Pinus bungeana ***,extract every tree’s data from the point cloud and measure the tree height and diameter at breast height（DBH）.After make accuracy precision and correlation analysis with the measured data,it is showed that the method mentioned can be satisfied the accuracy requirement of forest investigation and can be used to measure the tree height and *** with traditional technology,the applications of 3D laser scanning technique have a broad development space on tree measuring.

关键词： Stand Laser Scanner Point Clouds Accuracy Precision

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The stability safety factor calibration based on the reliability index

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engineering Sciences 2014年第3期12卷 65-69页

作者： Tong Xiaolong Fang Zhi College of Civil engineering Hunan University Changsha 410082 China School of Civil engineering Hunan Institute of Science and Technology Yueyang Hunan 414006 China

When the bridge structure stability safety factor of the first type is 4. the research that whether the structure reliability index will reach target reliability index under the more-likely-to-happen collapse situation of the second type is necessary. The stability calculations of the first and the second type are made respectively for single layer and single span rigid frame bridge. Based on the critical load obtained from the stability calculation of the first type, the stability safety factor of the first type is taken as 4. and the first order reliability method is used to program and calculate the reliability index. Then, the load effect under the stability reliability index cal- culation of the first type and the critical load of the second type are employed to calculate the reliability index of the second type. The evaluation of structure stability safety factor is discussed according to reliability index. Based on the discussion above, parameter analysis of the stable critical loads of two types is made, and the in- fluence of critical load change on reliability index is researched. The result shows that stability analysis should identify collapse state; when the stability safety factor of the first type is 4. but the structure has the collapse of the second type, the reliability index cannot be ensured to reach the target reliability index under certain condi- tions.

关键词： stability safety factor reliability index rigid frame bridge

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湖南地区汉族人群中HLA-A、B、DRB1等位基因与原因不明习惯性流产相关性研究

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现代免疫学 2009年第6期29卷 497-500页

作者：钱小兵程腊梅谢毓滨周佳金文静卢光琇中国科学院研究生院北京100049 人类干细胞国家工程研究中心长沙410078 中南大学生殖与干细胞工程研究所长沙410078 长沙血液中心长沙410001

探讨HLA-A、-B和-DRB1位点等位基因多态性与原因不明习惯性流产的相关性。应用PCR-SSP方法对175对(350例)湖南汉族原因不明习惯性流产(URSA)患者夫妇进行HLA-A、-B、-DRB1基因分型,并以2.000例(男女各1 000例)湖南无关健康汉族个体造血... 详细信息

探讨HLA-A、-B和-DRB1位点等位基因多态性与原因不明习惯性流产的相关性。应用PCR-SSP方法对175对(350例)湖南汉族原因不明习惯性流产(URSA)患者夫妇进行HLA-A、-B、-DRB1基因分型,并以2.000例(男女各1 000例)湖南无关健康汉族个体造血干细胞分型资料作为对照,分析HLA等位基因频率在两组中的分布差异。结果是HLA-A、-B及-DRB1位点各等位基因频率在对照组男女中均无显著差异(P>0.05);URSA患者组A*31(2.86%)、B*08(0.86%)、B*39(4.14.)和B*56(1.86%)的等位基因频率显著高于对照组(RR值分别为2.07、2.99、1.56和2.19;P值分别为0.0059、0.02.6、0.04.4.0.0172.;在URSA妻子组中,B*39(4.57%)显著高于对照组(RR=1.75;P=0.04.4.,A*33(3.71%)、B*58(3.14.)和DRB1*03(2.2.%)的等位基因频率明显低于对照组(RR值分别为0.57、0.51和0.50;P值分别为0.0339、0.0182.0.0383);A*31(3.14.)、B*08(1.4.%)、B*52.2.2.%)及B*56(2.2.%)在URSA丈夫组中的等位基因频率均高于对照组(RR值分别为2.32.5.08、2.31和2.77;P值分别为0.013、0.0012.0.0374.0.0105)。结果表明湖南汉族人群中A*31、B*08、B*39、B*52.B*56可能是URSA的易感等位基因,而A*33、B*58和DRB1*03可能对URSA的发生有保护作用。

关键词：原因不明习惯性流产人类白细胞抗原等位基因频率

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Multilevel Recycling Waste Water of Raising Pigs Based on Local Farming Model and Its Effect Evaluation

Multilevel Recycling Waste Water of Raising Pigs Based on Lo...

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2.12.环境污染与大众健康学术会议

作者： Yuandong XIAO 1,2., Xiaozhen LIU 3,4., Chunli LUO 1 , Yue LIANG 3,4.,Xu CHEN 1 ,Ming XU 3,4.1、College of Land Resource and Environment, Jiangxi Agricultural University, Nanchang 33004., P.R. china 2.Agriculture and Ecology engineering research center of Po-yang Lake Watershed,Nanchang 33004., Peoples Republic of china 3、Key Lab. of Poyang Lake Environment and Resource Utilization, Nanchang University, Ministry of Education, Nanchang 33004., P.R.china 4. College of Environmental Science and engineering, Nanchang University, Nanchang 330031, P.R. china

A large-scale pig farms in Jiangxi Province was researched ,a model of resource utilization for breeding wastewater was established with methane tank →clarifier →reservoir breeding →farmland irrigation. The results showed that the concentrations of COD, ammonia nitrogen and total phosphorus of the treated wastewater were 59.13mg/L,1.15mg/L and 0.015mg/L respectively , the degradation rates of which were 2.1}2.,99.34.,93.85% separately. The fish bait of reservoir for fish culture was saved by discharging certain concentration wastewater into it while the organic matter in the aquiculture wastewater was degraded by ecosystem. The reservoir water was used later as irrigation water and organic matter in the aquiculture wastewater was degraded thoroughly, which decreased the cost of discharging sewage from farms, eliminating pig emission pollution to the environment and increasing crop yields. The above model was an ecomodel, not only treating cultivating wastewater but also recycling the waste water. The management of piggery wastewater and monitoring of reservoir water quality were suggested in order to avoid water eutrophication.

关键词： Piggery wastewater Reservoir Field irrigation Resource recycling

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Epstein-Barr virus-encoded latent membrane protein 1 modulates cyclin D1 by c-Jun/Jun B heterodimers

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Science china(Life Sciences) 2005年第4期48卷 385-393页

作者： SONG Xin1, TAO Yongguang1, ZENG Liang1, YANG Jing1, TANG Faqing1, Leo M. Lee2. GONG Jianping3, WU Qiao4.& CAO Ya1 1. Cancer research Institute, Xiangya School of Medicine, Central South University, {2. 4.0078, china 2. Laboratory of Molecular Technology SAIC-Frederick, National Cancer Institute P.O. Box B, Frederick, MD 2.702. USA 3. Molecular Medical center, Tongji Hospital, Tongji Medical University, Wuhan 4.0030, china 4. Key Laboratory of the Ministry of Education for Cell biology, and Tumor Cell engineering, School of Life Sciences, Xiamen University, Xiamen 361005, china 1. Cancer research Institute Xiangya School of Medicine Central South University 410078 Changsha China 2. Laboratory of Molecular Technology SAIC-Frederick National Cancer Institute P.O. Box B 21702 Frederick MD USA 3. Molecular Medical center Tongji Hospital Tongji Medical University 430030 Wuhan China 4. Key Laboratory of the Ministry of Education for Cell biology and Tumor Cell Engineering School of Life Sciences Xiamen University 361005 Xiamen China

In our recent studies, we found that LMP1 encoded by Epstein-Barr virus could accelerate the formation of active c-Jun/Jun B heterodimer. We studied the regulation of cyclinD1 by c-Jun/Jun B heterodimers by laser scanning confocal influorescence microscopy, Western blot, luciferase activity assay, super-EMSA and flow cytometry in the Tet-on-LMP1 HNE2.cell line, in which LMP1 expression was regulated by Tet-on system. c-Jun/Jun B heterodimers induced by LMP1 could up regulate cyclin D1 promoter activity and expression. Overexpression of cy-clinD1 accelerated the progression of cell cycle.

关键词： latent membrane protein1, c-Jun, Jun B, heterodimer, cyclin D1, cell cycle.

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