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Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon

Chinese Science Bulletin 2006年第17期51卷 2059-2064页

作者： LIU Bin1,2.3,4. WANG Kemin1,2.3,4. XIAO Zhiqiang5,6, WANG Wei1,3,4. TAN Weihong1,2.3,4. SUN Yi5,6, TANG Hongxing1,3,4.& YANG Xiaohai1,3,4.. State Key Laboratory of Chemo/biosensing and Chemometrics, Hu- nan University, changsha 410082. china 2. Life Science and biotechnology Institute, hunan University, Chang- sha {4. china 3. Chemistry and Chemical Engineer College, hunan University, changsha 410082. china 4. hunan engineering research center for bio-nanotechnology, hunan University, changsha 410082. china 5. Medical Experiment research center, Xiangya Hospital, Central South University, changsha 4.0008, china 6. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, changsha 4.0008, china State Key Laboratory of Chemo/biosensing and Chemometrics Hunan University Changsha China Life Science and biotechnology Institute Hunan University Changsha China Chemistry and Chemical Engineer College Hunan University Changsha China hunan engineering {2. center for {4.-nanotechnology Hunan University Changsha China Medical Experiment research center Xiangya Hospital Central South University Changsha China Key Laboratory of Cancer Proteomics of Chinese Ministry of Health Changsha China

The effects of drug treatment, gene transfection, and p53 RNA interference (RNAi) on level of ING1 mRNA in tumor cells were quantitatively detected with the help of ING1 molecular beacon (MB)/cRNA standard curve based on MB detection technology. Results showed that the level of ING1 mRNA could be upregulated by 5-FU treatment or ING1 transfection in low expression cell line of MCF-7 and be inhibited by silencing p53 with RNAi technology in normal expression cell line of CNE2. The level of ING1 varied from 7.7×10?16 to 53.4. 10?16 mol/μg total RNA of tumor cells. The results not only provided evidence for the regulation effects of gene expression but also were applied to investigat- ing the interaction of multigenes on signal transduc- tion pathway.

关键词： ING1 RT-PCR 定量分析分子信号 mRNA

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Microstructure and formation mechanism of twins of laths of austenite with high nitrogen

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Science china(Technological Sciences) 2007年第6期50卷 755-764页

作者： WU CuiLan1,2.& LUO ChengPing2.1 center for High Resolution Electron Microscopy, College of Materials Science and engineering, {2. University, changsha 410082. china 2.College of Mechanical engineering, South china University of Technology, Guangzhou 51064., china 1. center for High Resolution Electron Microscopy College of Materials Science and Engineering Hunan University Changsha 410082 China 2. College of Mechanical engineering South China University of Technology Guangzhou 510641 China

The microstructure of composite diffusion layer of the nitrided and chromized 0.2. carbon steel is investigated using TEM and EDS. It is found that laths of austenite with high nitrogen (γ N) precipitate from α-ferrite matrix in the deeper zone of the diffusion layer. These γ N laths are all twins, with their {111} twinning planes parallel to the lath axis, thus forming a characteristic "back-to-back" morphology. There are two types of γN lath. The first is a genuinely {111} twin, and γ N and α keep the accurate K-S relationship, and each γ N and α form a sharp and smooth γ N/α inter- face of {335}γN//{341}α, namely habit plane {335}fcc. The second is a pseudo-twin, with micro-twins {111} or faults formed within the two twin components. Localized lattice deformation (relaxation) seems to have occurred at the interfaces of the second type of γ N due to the formation of micro-twins or faults within the twin components. These micro-twins or faults make the orientation relationship (OR) between each of the γN and the α-matrix deviate from the accurate K-S OR, and the OR between two γ N twin components deviate from the genuine {111} twin relation- ship. In addition, the γ N/α interface of the second type of γ N is not as sharp or smooth as that of the first one.

关键词： austenite containing nitrogen, twins, steel 2., nitriding, composite chromizing

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Physicochemical and microbiological effects of biosurfactant on the remediation of HOC-contaminated soil

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Progress in Natural Science:Materials International 2005年第7期15卷 577-585页

作者： ZENG Guangming , ZHONG Hua, HUANG Guohe and FU Haiyan ( Department of Environmental Science and engineering, hunan University,{2. Faculty of engineering, University of Regina, Regina, Saskatoon S4.0A2. Canada) Department of Environmental Science and engineering Hunan University Changsha 410082 China. Faculty of engineering University of Regina Regina Saskatoon S4S0A2 Canada

Remediation of soil contaminated by hydrophobia organic compounds using biosurfactants as additives involves interactions between soil matrix, hydrophobic organic compound contaminants, biosurfactants and microorganisms. In this paper, the mechanism for biosurfactants to enhance the contaminant degradation is basically revealed. biosurfactants can enhance solubilization of the contaminants in the soil matrix, change their mass transfer properties into the aqueous phase, as well as affect their sorption properties. Furthermore, biosurfactants can act on microorganisms and change their surface properties, accordingly cause new growth and uptake behavior of the bacteria in the soil matrix. Both the physicochemical and the microbiological effects can basically increase the bioavailability of the contaminants and enhance their degradation.

关键词： bioavailability physicochemical effects microbiological effect hydropbobic organic compounds biosurfactants.

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Aspects of recent developments in analytical chemometrics

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Science china Chemistry 2006年第3期49卷 193-203页

作者： LIANG Yizeng1,2. WU Hailong1, SHEN Guoli1, JIANG Jianhui1, LIANG Sheng2.& YU Ruqin1 1. State Key Laboratory of Chemo/biosensing and Chemometrics, College of Chemistry and Chemical engineering, {2. University, changsha 410082. china 2. {4. center of Modernization of Traditional Chinese Medicines, Central South University, changsha 4.0081, china 1. State Key Laboratory of Chemo/biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University Changsha 410082 China 2. research center of Modernization of Traditional Chinese Medicines Central South University Changsha 410081 China

Some aspects of recent developments in analytical chemometrics are discussed, in par- ticular the developments viewed from the angle of the research efforts undertaken in authors’ labora- tories. The topics concerned include resolution of high-order chemical data, morphological theory and methodology for chemical signal processing, multivariate calibration and chemical pattern recognition for solving complex chemical problems, and resolution of two-way chemical data from hyphenated chromatographic instruments.

关键词： chemometrics, high-order chemical data, pattern recognition, multivariate calibration and resolution.

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Synthesis and magnetic properties of CdS/α-Fe_2._3 hierarchical nanostructures

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Science china(Physics,Mechanics & Astronomy) 2009年第7期52卷 997-1002页

作者： CHEN YuJin1,2.3, SHI XiaoLing1, CAO MaoSheng1 & ZHU ChunLing4.1 School of Materials Science and engineering, Beijing Institute of Technology, Beijing 100081, {2. 2.Micro-Nano Technologies research center, {4. University, changsha 410082. china 3 College of Science, Harbin engineering University, Harbin 150001, {2. 4.College of Material Science and Chemical engineering, Harbin engineering University, Harbin 150001, {2. 1. School of Materials Science and engineering Beijing Institute of Technology Beijing 100081 China 2. Micro-Nano Technologies research center Hunan University Changsha 410082 China 3. College of Science Harbin Engineering University Harbin 150001 China 4. College of Material Science and Chemical engineering Harbin Engineering University Harbin 150001 China

CdS/α-Fe2.3 hierarchical nanostructures, where the CdS nanorods grow irregularly on the side surface of α-Fe2.3 nanorods, were synthesized via a three-step process. The diameters and lengths of CdS nanorods can be tuned by changing the ethylenediamine (EDA) and Cd ion concentrations. The magnetic investigations by superconducting quantum interference device indicate that the hierarchical nanostructures have an Morin transition at lower temperature (2.0 K) than that of the single bulk α-Fe2.3 materials (2.3 K). Importantly, the hierarchical nanostructures exhibit weakly ferromagnetic characteristics at 300 K. A sharp peak assigned to the surface trap induced emission are observed in room temperature PL spectra. Combining with the optoelectronic properties of CdS, the CdS/ α-Fe2.3 hierarchical nanostructures may be used as multi-functional materials for optoelectronic and magnetic devices.

关键词： α-Fe2.3 CdS hierarchical nanostructures magnetic properties

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One-pot synthesis of crystalline SnO_2.nanoparticles and their low-temperature ethanol sensing characteristics

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Science china(Physics,Mechanics & Astronomy) 2009年第10期52卷 1601-1605页

作者： CHEN YuJin1,2.3,ZHU ChunLing4.WANG LiJiao3 & WANG TaiHong2.1 School of Materials Science and engineering,Beijing Institute of Technology,Beijing 100081,{2. 2.Micro-Nano Technologies research center,{4. University,changsha 410082.{2. 3 College of Science,Harbin engineering University,Harbin 150001,{2. 4.College of Material Science and Chemical engineering,Harbin engineering University,Harbin 150001,{2. School of Materials Science and engineering Beijing Institute of Technology Beijing 100081 China Micro-Nano Technologies research center Hunan University Changsha 410082 China College of Science Harbin Engineering University Harbin 150001 China College of Material Science and Chemical engineering Harbin Engineering University Harbin 150001 China

Crystalline SnO2.nanoparticles(NPs) with a diameter less than 6 nm are synthesized using potassium stannate trihydrate as the precursor in a basic *** synthesized NPs can detect ethanol at a ppm level even at 100℃.Furthermore,the NPs have good selectivity to *** excellent ethanol sensing performances are attributed to the small size effect according to the space-charge model.

关键词： SnO2 nanoparticles sensing characteristics

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Experimental Study of Sediment Incipience Under Complex Flows

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Transactions of Tianjin University 2008年第4期14卷 300-306页

作者： LIU Chunrong DENG Liying HUANG Zhenhua HUHE Aode Maritime research center School of Civil and Environmental Engineering Nanyang Technological University Singapore 639798 College of Mechanics and Aerospace Hunan University Changsha 410082 China School of Civil and Environmental engineering Nanyang Technological University Singapore 639798 Institute of Mechanics Chinese Academy of Sciences Beijing 100080 China

Sediment incipience under flows passing a backward-facing step was studied. A series of experiments were conducted to measure scouring depth, probability of sediment incipience, and instantaneous flow velocity field downstream of a backward-facing step. Instantaneous flow velocity fields were measured by using Particle Image Velocimetry (PIV), and an image processing method for determining probability of sediment incipience was employed to analyze the experimental data. The experimental results showed that the probability of sediment incipience was the highest near the reattachment point, even though the near-wall instantaneous flow velocity and the Reynolds stress were both much higher further downstream of the backward-facing step. The possible me- chanisms are discussed for the sediment incipience near the reattachment point.

关键词： sediment transport local scour complex flows particle image velocimetry sediment incipience

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TEM STUDY OF C-B_4.-SiC COMPOSITES WITH SILICON ADDITIVE

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Acta Metallurgica Sinica(English Letters) 1995年第2期8卷 92-96页

作者： ZHAO Lihua WU Lijun HUANG Qizhong YANG Qiaoqin 1)(Material research and Test center,{2. University,changsha,{4.)2.(Powder Metallurgy research Institute,center-South University of Technology,changsha,{4.)3)(Laboratory of Atomic Imaging of Solids, Ins hunan Univ Changsha China

C-B4.-SiC composites with silicon additive obtained by the hot press sintering method are studied by means of transmission electron microscopy(TEM).When the specimens are heated to 1800℃,their structure does not *** the specimens are heated to 2.00℃,carbon begins to graphitize. Moreover,two monoclinic phases are *** monoclinic phase named as the M phase belongs to C-center monoclinic lattice with a=0.90nm,b=0.59nm,c=0.54.m and β=119.3°.The other monoclinic phase named as the Ml phase also belongs to C-center monoclinic lattice but with a=0.85nm,b=0.4.nm,c=0.53nm and β=12..6°.

关键词： C-B_4.-SiC composite electron microscopy

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Residual stress and radial stress gradient in polycrystalline diamond compacts

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金刚石与磨料磨具工程 2008年第S1期28卷 88-91,96页

作者： Xu Guoping~(1,2.,Yin Zhimin~1,Chen Qiwu~2.Xu Gen~1 (1.School of Materials Science & engineering,Central South University,changsha {2.0083,hunan,china) (2.changsha research institute of mining and metallurgy,changsha {2.0012.hunan,china)

The differential thermal expansion of the polycrystalline diamond layer and the tungsten carbide substrate results in large residual stresses as PDC cutters cooling after *** residual stresses on the top surface of the diamond layer of PDC were measured at five points along the radial direction of PDC using X-ray Diffraction Residual Stress Instrument,thus the stresses and their radial distribution were *** results show that the stresses on the diamond surface are compressive,the biggest stress appears at the central point(about 12.0 MPa),and that from the center to the edge of PDC,the magnitude of the stress decreases. A finite element analysis(FEA) was made to check the validity of the testing *** FEA modeling results were found to correlate well with the measured *** leading to the deviation between XRD experimental measurements and the calculations of residual stress by FEA were also analyzed.

关键词： Polycrystalline diamond Residual stress XRD FEA

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LOD score exclusion analyses for candidate disease susceptibility genes using case-parents design

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Progress in Natural Science:Materials International 2006年第1期16卷 30-38页

作者： DENG Hongwen1,2.3 and GAO Guimin2.4.(1.Laboratory of Molecular and Statistical Genetics, College of Life Sciences, hunan Normal University, {2. 4.0081, china 2.Osteoporosis research {4., Creighton University Medical {4., Omaha NE 68131, USA 3. Department of biomedical Sciences, Creighton University, Omaha NE 68131, USA 4. Virginia bioin format ics Institute and Department of Statistics, Virginia Polytechnic Institute and State University, Blacksburg VA 2.0}61-0315, USA) [a]Laboratory of Molecular and Statistical Genetics College of Life Sciences Human National University Changsha China [b]Osteoporosis research center Creighton University Medical Center Omaha NE USA [c]Department of biomedical Sciences Creighton University Omaha NE USA [d]Virginia bioinformatics Institute and Department of Statistics Virginia Polytechnic Institute and State University Blacksburg VA USA

The focus of almost all the association studies of candidate genes is to test for their importance. We recently developed a LOD score approach that can be used to test against the importance of candidate genes for complex diseases and quantitative traits in random samples. As a complementary method to regular association analyses, our LOD score approach is powerful but still affected by the population admixture, though it is more conservative. To control the confounding effect of population heterogeneity, we develop here a LOD score exclusion analysis using case-parents design, the basic design of the transmission disequilibrium test (TDT) approach that is immune to population admixture. In the analysis, specific genetic effects and inheritance models at candidate genes can be analyzed and if a LOD score is ≤ - 2. 0, the locus can be excluded from having an effect larger than that specified. Simulations show that this approach has reasonable power to exclude a candidate gene having small genetic effects if it is not a disease susceptibility locus (DSL) with sample size often employed in TDT studies. Similar to association analyses with the TDT in nuclear families, our exclusion analyses are generally not affected by population admixture. The exclusion analyses may be implemented to rule out candidate genes with no or minor genetic effects as supplemental analyses for the TDT. The utility of the approach is illustrated with an application to test the importance of vitamin D receptor (VDR) gene underlying the differential risk to osteoporosis.

关键词： association studies candidate genes case-parents design disease susceptibility locus exclusion mapping LOD score.

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