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第十二届全国植物基因组学大会

作者： Junping Shi,Lu Wang,Bin Han~*,Ahong Wang National center For Gene Research and Institute of Plant Physiology and Ecology,shanghai institutes of biological {4., chinese academy of sciences,shanghai 200233,china

Rice(Orvza sativa L.) is one of human’s major food staples,and 1,000-grain weight(TGW) which is determined by grain length,width,thickness and filling rate is a vital component of grain *** identify the genes conditioning this trait and reveal the molecular mechanism of them,we carried out a quantitative trait locus(QTL) mapping with 150 rice recombinant inbred lines(RILs) which were developed from a cross between Nipponbare(japonica,low TGW) and 93-11(indica,high TGW).4.QTLs were detected;two localized on chromosome 5,the other two localized on chromosome 8 and 10,*** we focused on a ma-

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香樟树种子中两种Ⅱ型核糖体失活蛋白凝集素活性的研究

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生物化学与生物物理进展 2004年第7期31卷 650-654页

作者：杨海燕李荣秀刘望夷中国科学院上海生命科学研究院生物化学与细胞生物学研究所中国科学院上海生命科学研究院生物工程研究中心

从香樟树成熟的种子分离到两种新的Ⅱ型核糖体失活蛋白 :新丰毒蛋白和辛纳毒蛋白 .两者A链的分子质量虽然相差近一倍 ,但B链的分子质量相同 .Ⅱ型核糖体失活蛋白的A链是RNAN 糖苷酶 ,B链是凝集素 ,A链进入细胞表现毒性在很大程度上依赖... 详细信息

从香樟树成熟的种子分离到两种新的Ⅱ型核糖体失活蛋白 :新丰毒蛋白和辛纳毒蛋白 .两者A链的分子质量虽然相差近一倍 ,但B链的分子质量相同 .Ⅱ型核糖体失活蛋白的A链是RNAN 糖苷酶 ,B链是凝集素 ,A链进入细胞表现毒性在很大程度上依赖于B链的糖结合活性和特异性 .对辛纳毒蛋白和新丰毒蛋白B链的凝集素活性进行了测定和比较 .红细胞凝集实验显示辛纳毒蛋白和新丰毒蛋白具有相同的细胞凝集活性 ,半抗原抑制实验发现它们都属于半乳糖型核糖体失活蛋白 ,荧光光谱法显示它们的糖结合常数也相同 .

关键词：新丰毒蛋白辛纳毒蛋白凝集素荧光光谱法

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利用肠激酶加工融合蛋白的方法制备重组人甲状旁腺素(1～34.肽

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生物化学与生物物理学报 2002年第4期34卷 469-474页

作者：修朝阳周贺钺俞璎戴金凤陈常庆中国科学院上海生命科学研究院生物工程研究中心中国科学院上海生命科学研究院生物工程研究中心上海200233

采用全基因分段合成和补丁连接相结合的方法构建了人甲状旁腺素 (hPTH) (1～ 34.肽基因 ,并利用GST融合表达系统 ,在大肠杆菌中克隆和可溶性表达了hPTH(1～ 34.肽基因。融合蛋白表达量占菌体总蛋白质的 2 5 %以上 ,经高密度发酵、亲和... 详细信息

采用全基因分段合成和补丁连接相结合的方法构建了人甲状旁腺素 (hPTH) (1～ 34.肽基因 ,并利用GST融合表达系统 ,在大肠杆菌中克隆和可溶性表达了hPTH(1～ 34.肽基因。融合蛋白表达量占菌体总蛋白质的 2 5 %以上 ,经高密度发酵、亲和纯化后 ,在每升发酵液中得到了 10 g融合蛋白。融合蛋白经肠激酶一步加工并亲合纯化和反相脱盐后 ,最终可以得到约 0 .6g /L人甲状旁腺素 (1～ 34.肽纯品 ,样品的理论回收值达到了4.8.75 %。产物的纯度和性质经HPLC、毛细管电泳、质谱分析、N端测序等得到证明 ,并与化学合成产物的结果相吻合。兔肾皮质细胞测活表明 ,重组产物与化学合成人甲状旁腺素 (1～ 34.肽具有相近的腺苷酸环化酶激活活性。

关键词：甲状旁腺素肠激酶脱水糜蛋白酶亲和柱亲和纯化

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HRCA技术在转基因植物检测中的应用

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生物工程学报 2003年第3期19卷 294-300页

作者：陶震蔡兴锋颜志强胡小波杨胜利龚毅中国科学院上海生命科学研究院生物工程研究中心上海200233

超分支滚环扩增技术 (Hyperbranchedrollingcycleamplification ,HRCA)在近几年中逐渐引起人们的注意 ,并越来越多的用于基础研究和实际检测中。在本文中我们对该技术在转基因植物检测中的应用情况作了较全面的探索 ;根据 4.转基因植... 详细信息

超分支滚环扩增技术 (Hyperbranchedrollingcycleamplification ,HRCA)在近几年中逐渐引起人们的注意 ,并越来越多的用于基础研究和实际检测中。在本文中我们对该技术在转基因植物检测中的应用情况作了较全面的探索 ;根据 4.转基因植物中常用的外源基因或DNA片段设计了 4.锁式探针 (Padlockprobe) ,利用质粒pKK2 32 8中的一段序列作为锁式探针中的共同连接部分 ,并根据该共同的连接部分序列设计一对通用的HRCA引物 ;利用同位素标记的锁式探针对HRCA反应中的连接一步的特异性研究表明 ,只有当锁式探针和相应的检测靶DNA同时存在于连接体系中时 ,锁式探针才能被有效地进行连接 ,从线性分子变为环型分子 ,在没有相应靶DNA存在时锁式探针仅以线性形式存在 ;连接时间的研究表明 ,如果所检测的靶DNA是质粒或较短的DNA片段时 ,较短的连接时间 (5～ 10min)就可以取得理想的最终检测效果 ,如果检测的靶DNA是复杂的植物基因组DNA时 ,连接时间需要较大程度的延长 (30～ 6 0min)才能取得理想的最终检测结果 ;HRCA的反应时间研究表明 ,较长的反应时间可以明显增加最终产物的量 ;对BstDNA聚合酶大片段酶用量的研究表明 ,在其它条件不变的情况下酶的用量可以在较大的范围内变化 (0 .5u～ 4.)而不影响最终检测结果 ;?

关键词： HRCA技术转基因植物检测锁式探针

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Neuron-specific knockout of NRSF mouse is more vulnerable to the dopaminergic neurotoxin MPTP

Neuron-specific knockout of NRSF mouse is more vulnerable to...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Mei YU~(1,#),Ming LIU~(2,#),Hai-Yun SUO~1,Lei CAI~2,Jian FEI~(3,4.*) Fang HUANG~(1,*) 1 National Key Laboratory of Medical Neurobiology,shanghai Medical College,Fudan University,shanghai 200032, china 2 institutes of Biochemistry and Cell Biology,shanghai institutes for biological sciences,chinese academy of sciences 3 School of Life Science and Technology,Tongji Universities,shanghai 200092,china 4.shanghai Nan Fang Model Organism Research {14.,shanghai 201203,china

Recent studies on neuron-restrictive silencer factor(NRSF) / neuronal repressor element-1 silencing transcription factor(REST) have revealed that NRSF participates in the pathological processes of nervous system diseases,such as global ischemia,epilepsy and Huntington *** l-methyl-4.phenyI-pyridinium ion(MPP) treatment triggers the mRNA and protein expression of NRSF in human dopaminergic SH-SY5Y cells,and alteration of NRSF expression exacerbates MPP-induced cell ***,we generated a neuron-specific conditional knockout mice of NRSF(cKO) by crossing NRSF floxed mice with the neuron-specific enolase(NSE)-Cre transgenic *** or wild type mice were injected with l-methyl-4.phenyl-l,2,3,6-tetrahydropyridine(MPTP) at a dose of 15mg/kg,four times within two days,and behavioral assays were performed respectively on 1,3,7,14.21 and 28 days after the last MPTP *** were sacrificed one day after the last behavioral *** levels of DA,its metabolite 3,4.dihydroxiphenylacetic acid (DOPAC),and 5-hydroxytryptamine(5-HT) or non-relevant 5-hydroxyindoleacetic acid(5-HIAA) in striatum were analyzed by ***,the protein level of tyrosine hydroxylase(TH) in striatum was detected by Western blotting *** results showed that in the experiment of swim test,the force swimming time in both cKO and wild type mice was obviously decreased after MPTP administration,but the rate of decline in the cKO mice was higher than that in the wild type *** intoxication reduced the levels of DA,DOPAC and 5-HT in cKO and wild type mice significantly. The ratio of decrease in the cKO mice was more dramatic than that in the wild type *** the same situation happened at the level of TH in *** results suggested that neuron-specific knockout of NRSF mouse is more vulnerable to the dopaminergic neurotoxin MPTP.

关键词： NRSF MPTP conditional knockout mouse NSE promoter Parkinson’s disease

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Lipid rafts mediates ATP-induced endocytosis,signal transduction and retrograde transport of P2X3 receptor

Lipid rafts mediates ATP-induced endocytosis,signal transduc...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Xu-Qiao CHEN~(1,#),Bin WANG~(1,#),Chengbiao WU~2,Jin PAN~1,Bo YUAN~3,Yuan-Yuan SU~1,Xing-Yu JIANG~3,Xu ZHANG~4.Lan BAO~(1,*) 1 Laboratory of Molecular Cell Biology,Institute of Biochemistry and Cell Biology,shanghai institutes for biological sciences,{4. academy of sciences,shanghai 2.0031,china 2 Department of Neurosciences,School of Medicine,University of California,San Diego,La Jolla,California,USA 3National center of Nanoscience and Technology,Beijing 100190,china 4.Institute of Neuroscience and State Key Laboratory of Neuroscience,shanghai institutes for biological sciences, chinese academy of sciences,shanghai 2.0031,china

P2X receptors are a family of cation channels expressed in neurons and nonneuronal *** play a variety of roles including synaptic transmission,muscle contraction,neuronal secretion and inflammation response. Among P2X receptors,the P2X subtype is selectively expressed in dorsal root ganglion *** raft is a specialized membrane microdomain and participate many signal transduction *** report showed that P2X receptor is localized in the lipid rafts of DRG *** have found P2X receptor engaged in retrograde transport in the form of signaling *** we investigated the role of lipid rafts in a,B-MeATP-dependent endocytosis and signal transduction,and retrograde transport of P2X *** of lipid raft with MBCD or nystatin abolished ATP-dependent endocytosis of P2X receptor and ATP-induced ERK activation in P2X receptor-expressing HEK293 *** vivo sucrose gradient fractionation with vesicular pellets prepared from sciatic nerve axoplasm showed that abundant P2X receptor was localized in lipid raft *** transported P2X receptors were colocalized with the lipid raft marker caveolinla in sciatic *** terminal treatment with MPCD attenuated retrograde transport of P2X receptor in axon of dorsal root ganglion ***,lipid raft mediates ATP-induced endocytosis,signal transduction and retrograde transport of P2X receptor.

关键词： P2X3 receptor lipid raft endocytosis ERK retrograde transport

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ATP-dependent retrograde axonal transport of P2X3 receptor in primary sensory neurons

ATP-dependent retrograde axonal transport of P2X3 receptor i...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Bin WANG~1,Xu-Qiao CHEN~1,Cheng-Biao WU~2,Jin PAN~1,Bo YUAN~3,Yuan-Yuan SU~1,Xing-Yu JIANG~3, Xu ZHANG~4.Lan BAO~(1,*) 1 Laboratory of Molecular Cell Biology,Institute of Biochemistry and Cell Biology,shanghai institutes for biological sciences,{4. academy of sciences,shanghai 2.0031,china 2 Department of Neurosciences,School of Medicine,University of California,San Diego,La Jolla,California 92093- 0662,USA 3 National center for Nanoscience and Technology,Beijing 100080,china 4.Institute of Neuroscience and State Key Laboratory of Neuroscience,institutes for biological sciences,{4. academy of sciences,shanghai 2.0031,china

Objective P2X receptors are ATP-gated cation channels and play variable roles in pathophysiological *** P2X receptors,P2X

关键词： primary sensory neuron P2X3 receptor axonal retrograde transport

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A Report of Oral Dose Toxicity Study of Triptolides （Tx） in Sprague-Dawley Rat

A Report of Oral Dose Toxicity Study of Triptolides （Tx） i...

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第十五届国际药理学大会

作者： Yong - Zhen Liu,Hui Wang,Li - Kun Gong,Ming Du,Jun - Liang Wu,Mei - Ying Wang. Heng - Lei Lu,Hua Sheng. Xiang - Hong Li,Jin Ren center for Drug Safety Evaluation and Research,shanghai Institute of Materia Medica, shanghai institutes for biological sciences,chinese {4. of sciences, shanghai 201203,china

＜正＞ Tx is a novel immunosuppressant derived from triptolides. To detect the no observed adverse effects level （NOAEL） and the toxic target organ of Tx. SD rats were orally administrated daily for 28 days following 28 days recovery period after withdrawal. 4. females and 4. males were randomly assigned to 4.groups respectively. The acute toxicity（LD50） of Tx in rats was 10mg/kg in females and 23mg/kg in males, indicating the different toxic sensitivity on rats in both sexes. The study was designed as for females at doses of 0,1,2 and 4.mg/kg,while males at 0,2,4.and 8 mg/kg. There were no drug - related changes in females at Img/kg group. However,females at 2 and 4.mg/kg and males at 4.and 8 mg/ kg groups showed obvious drug - related changes in the decreased body weight, hematology（decrease of erythrocyte count, hemoglobin and hematocrit,and increase of reticulocyte count and platelet）, and histopathology（spleen, testes and epididymis）. All toxic changes were dose- dependent. It is suggested that the NOAEL is 1 mg/kg in females and less than 2 mg/kg in males. The toxicity target organs of Tx were the spleen in both sexes and the reproductive system in males.

关键词： Triptolides NOAEL Toxicity

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No association between the FXYD6 gene and schizophrenia in the chinese Han Population

No association between the FXYD6 gene and schizophrenia in t...

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第八次全国医学遗传学学术会议(中华医学会2009年医学遗传学年会)

作者： Jing Zhang,Ronglin Che Xingwang Li~(1,3),Wei Tang~(1,3),Ruqi Tang~(1,3),Yang Wang~(1,3),Zhao Zhang~(1,3),Jue Ji~(1,3),Fengping Yang~(1,3),Yongyong Shi~(1,3),Weidong Ji~6,Guoquan Zhou~6,Guoyin Feng~4.Lin He~(2,3,5*),and Guang He~(1,3*) 1.Bio-X center,shanghai Jiao Tong University,1954.Huashan Road,shanghai 200030,P.R.china 2.{4. of Biomedical sciences,Fudan University,138 Yixueyuan Road,shanghai 200032,P.R.china 3.Institute for Nutritional sciences,shanghai {4. for biological sciences,chinese {14. of sciences, 294.Taiyuan Road,shanghai 2.0031,PR china 4.shanghai Institute of Mental Health,600 South Wan Ping Road,shanghai 200030,P.R.china 5.Bio-X Life Science center,shanghai Jiao Tong University,1954.Huashan Road,shanghai 200030,P.R. china 6.Institute of Neuropsychiatric Science and Systems biological Medicine,Changning Institute of Mental Health,the Bio-X center Hospital,shanghai,china

Choudhury et *** FXYD6 as a susceptible gene for schizophrenia in the London and Aberdeen *** genotyped D11S1998 and 8 SNPs(rs869789,rs11216567,rs10790212,rs876797,rs4.384.5,rs4.7768,rs11216598,rs11605223) in a chinese sample consisting of 1514.schizophrenia patients and 1514.healthy *** also compared the expression levels of FXYD6 in lymphocytes in 86 schizophrenia patients and 94.*** association was detected either in D11S1998 or the 8 SNPs. No difference was found in expression level between patients and *** study suggests that FXYD6 does not play a role in schiz-ophrenia in the chinese Han population.

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Structural insights into a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans

Structural insights into a dual-specificity histone demethyl...

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第十七届国际生物物理大会;第十二届中国生物物理大会

作者： Ying Yang~(1,2,3),Lu Zhou~5,Hanqin Lin~6,Degui Chen~6,Yanhui Xu~(1,2,3),Lulu Hu~(2,3),Ping Wang~(2,3),Haifeng Hou~4.Yan Lin~(2,3),Yi Liu~(2,3) 1.Cancer Institute,shanghai Cancer center,Fudan University Department of Oncology,shanghai Medical College,Fudan University,shanghai,{4. 2.School of Life sciences,Fudan University,220 Han-Dan Road,shanghai,{4. 3.institutes of Biomedical sciences,Fudan University,shanghai,{4. 4.Beijing Synchrotron Radiation Facility,Institute of High Energy Physics,chinese academy of sciences, Beijing,{4. 5.School of Pharmacy,Fudan Univercity,South Chemistry Building,Room 315,826 Zhangheng Road, shanghai,{4. 6.State Key Laboratory of Molecular Biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai,{4.

Histone lysine nethylation can be removed by JmjC domain-containing proteins in a sequence- and methylation-state-specific ***,how substrate specificity is determined and how the enzymes are regulated were largely ***... 详细信息

关键词： histone methylation demethylase PHD Jmjc

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