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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Song Yun, Sun Daye, Guo Yi* 1institute of molecular and Cell Biology, hebei normal university, shijiazhuang, P. R. china, 050016

＜正＞Plants have evolved sophisticated systems to cope with the adverse environmental conditions such as cold, drought and salinity due to their sessile nature. It has been reported that stress conditions have great ... 详细信息

关键词： Apoplastic proteins salt stress rice 2-D DIGE

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Analysis of Apoplast Calmodulin binding to Arabidopsis suspension-cultured cells1

Analysis of Apoplast Calmodulin binding to Arabidopsis suspe...

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中国植物生理学会第九次全国会议

作者： Sujuan Cui Xiaoqiang Guo Fang Chang Ying Sun Ligeng Ma & Daye Sun institute of molecular & Cellular Biology, hebei normal university, shijiazhuang 050016, china

＜正＞ Calmodulin, as important calcium-binding receptor protein, not only exists intracellular, but also extracellular in animal and plant system. By Western-blot assay, immonreacted calmodulin was first detected in extracelular of Arabidopsis suspension-cultured cells. 3H-TdR uptake of Arabidopsis suspension-cultured cells was promoted by exogenous purified calmodulin, and inhibited by anti-calmodulin serum and W7-agarose added into the cultured medium. Radioligand binding analysis showed there are specific, reverse and saturational

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植物类受体激酶CrRLK1-L亚家族及其生物学功能

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生物化学与生物物理进展 2011年第10期38卷 891-899页

作者：韩永峰杨倩张胜伟孙大业孙颖河北师范大学分子细胞生物学研究所河北省分子细胞生物学重点实验室石家庄050016

植物CrRLK1-L亚家族类受体激酶的胞外域具有新颖结构基序,但功能大都未知.该家族成员广泛存在于被子植物中,但在动物和微生物中不存在其同源物.CrRLK1-L家族成员相对较少,但组织表达非常广泛.它们定位于细胞质膜上,并且部分成员的定位... 详细信息

植物CrRLK1-L亚家族类受体激酶的胞外域具有新颖结构基序,但功能大都未知.该家族成员广泛存在于被子植物中,但在动物和微生物中不存在其同源物.CrRLK1-L家族成员相对较少,但组织表达非常广泛.它们定位于细胞质膜上,并且部分成员的定位还具有极性,这与其参与雌雄配子体的识别和受精作用密切相关.该家族成员普遍具有激酶活性,该活性对其功能的发挥至关重要.目前仅报道在拟南芥中参与助细胞与花粉的识别和调控营养组织的细胞伸长,但参与这些生物学过程的作用机制似乎独立于已知的信号通路之外,可能有自身独特的信号传导机制.所以对这一类具特有结构基序的类受体激酶基因的功能研究,将有助于解析植物特有生物学过程的分子作用机制,特别是在植物有性生殖过程中,合理利用这些分子开展育种实践对未来农业生产具有潜在的应用价值.

关键词：类受体激酶 CrRLK1-L 信号传导

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维普期刊数据库

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Brain Iron Metabolism:Physiology & Pathophysiology

Brain Iron Metabolism:Physiology & Pathophysiology

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中国生理学会、中国神经科学会2005年神经内分泌免疫学术研讨会

作者： Zhong Ming QIAN, M.D., PhD.Laboratory of Brain Iron Metabolism, Department of Applied Biology & Chemical Technology, and National Key Laboratory of Chinese Medicine and molecular Pharmacology (Shenzhen), Hong Kong Polytechnic university, Hong Kong institute of neurobiology and Neuropharmacology, hebei normal university, shijiazhuang, hebei, PRC 050016 institute of Chemical biology and Pharmaceutical Sciences, Capital university of Medical Sciences, Beijing, PRC 100054

Abnormally high levels of iron in the brain have been demonstrated in a number of neurodegenerative disorders, such as Hallerorden-Spatz syndrome, Parkinson's Disease, and Alzheimer's disease. Oxidative stress, resulting from increased brain iron levels, and possibly also from defects in antioxidant defense mechanisms, is widely believed to be associated with neuronal death in these disorders. However, two tightly linked key questions have not been completely answered. First, why does kon increase abnormally in some regions of the brain in these disorders?

关键词： Brain Iron Metabolism:Physiology Pathophysiology

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BcLTP,a Novel Lipid Transfer Protein in Brassica chinensis,may Secrete and Combine Extracellular CaM

BcLTP,a Novel Lipid Transfer Protein in Brassica chinensis,m...

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中国细胞生物学学会第九次会员代表大会暨青年学术大会

作者： Chun-Ming Wang~1 Wan-Qin Xie~2 Cui-Feng Li~2 Ying Sun~1 1 institute of molecular Cell Biology,hebei normal university,shijiazhuang 050016,china 2 College of Life Science,Nankai university,Tianjin 300071,china

Lipid transfer proteins in plants are believed to be involved in many processes of cell physiology and development. In this work,a full length cDNA encoding a novel lipid transfer protein,designated BcLTP was isolated... 详细信息

关键词： lipid transfer protein CaM isoforms extracellular space

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Receptor-Iike Kinase OsCR4 and Two Extracellular Small Protein CIP1 and CIP2 Co-express in Reproductive Tissues

Receptor-Iike Kinase OsCR4 and Two Extracellular Small Prote...

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2009中国植物学会植物细胞生物学学术年会

作者： Cui-Xia Pu,Yun Ma,Ling-Ling Wang,Zheng-Ge Zhu,Da-Ye Sun, Ying Sun~* institute of molecular cell biology,hebei normal university,shijiazhuang,Henei province,050016,china

The receptor-like kinases CR4 and ACR4 are well known for their roles in epidemis cell differentiation and are essential for the formation of intact ***4, an orthologue of CR4 in rice(Oryza sativa),is also functional ... 详细信息

关键词： CIP1 CIP2 extracellular factors seeds development

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转铁蛋白受体2及其功能与相关疾病

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生物化学与生物物理进展 2003年第4期30卷 533-536页

作者：常彦忠段相林钱忠明香港理工大学应用生物及化学科技学系铁代谢实验室

转铁蛋白受体 2 (transferrinreceptor 2 ,TfR2 )是最近发现的一种重要铁代谢蛋白 .研究显示它不仅是一种介导肝脏细胞铁摄取的主要蛋白 ,而且在调节小肠铁吸收方面起着极其关键的作用 ,是控制肝脏铁调素合成和释放的关键成分 .已经证实... 详细信息

转铁蛋白受体 2 (transferrinreceptor 2 ,TfR2 )是最近发现的一种重要铁代谢蛋白 .研究显示它不仅是一种介导肝脏细胞铁摄取的主要蛋白 ,而且在调节小肠铁吸收方面起着极其关键的作用 ,是控制肝脏铁调素合成和释放的关键成分 .已经证实 ,TfR2基因突变是遗传性血色素沉着病的重要原因之一 .

关键词：转铁蛋白受体2 功能铁吸收铁代谢遗传性血色素沉着病

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维普期刊数据库

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Tandem Affinity Purification of BZR1 Interacting Protein Complex in Arabidopsis

Tandem Affinity Purification of BZR1 Interacting Protein Com...

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2006年拟南芥研究学术研讨会

作者： Wang Chunming, Wang Zhi-Yong, Sun Ying institute of molecular Cell Biology, hebei normal university, shijiazhuang, hebei, 050016, P. R. china Department of Plant Biology, Carnegie Institution, Stanford, CA 94305 USA

＜正＞Brassinosteroids （BRs） are essential for many aspects of plant growth and development. Two homologous transcription factors BZR1 and BES1/BZR2 have been demonstrated to be involved in receptor-kinase mediated BR signaling pathway by regulating BR response gene expression. Both BZR1 and BES1/BZR2 are negatively regulated by BIN2 kinase phosphorylation and activated by BR-induced dephosphorylation. To gain an insight into the BZR1 functional mechanism, we used a clone-based functional proteomic approach to analyze BZR1 interacting proteins. We first introduced affinity-tagging

关键词： Tandem Affinity Purification BZR1.

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Overexpression of a Wall-Associated Kinase OsWAK2 Causes Pleiotropic Phenotype in Rice

Overexpression of a Wall-Associated Kinase OsWAK2 Causes Ple...

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2009整合植物生物学研讨会

作者： Qian Zhang~(1,2+),Li-Jing Sun~(2+),Yi-Hong Yang~2,Ying Sun~(2*) and Tie-Gang Lu~1 1 Biotechnology Research institute/National Key Facility for Crop Gene Resources and Genetic Improvement,Chinese Academy of Agricultural Sciences,Beijing 100081,china 2 institute of molecular Cell Biology,hebei normal university,shijiazhuang 050016,china.

The wall-associated kinases(WAKs)play important roles in cell expansion,pathogen resistance,and heavy-metal responses in Arabidopsis ***2 is a typical member of this gene family in rice(Oryza sativa)that shows similarity to AtWAKs. OsWAK2-GFP fusion protein is localized on the cell surface and associated with the cell

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Transglutaminase 3 expression in C57BL/6J mouse embryo epidermis and the correlation with its differentiation

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Cell Research 2005年第2期15卷 105-110页

作者： Jian ZHANG1,2, Hui Ying ZHI1, Fang DING1, Ai Ping LUO1, Zhi Hua LIU1*1National Laboratory of molecular Oncology, Cancer institute, Chinese Academy of Medical Sciences & Peking UnionMedical College, Beijing 100021, china.2Life Science institute, hebei normal university, shijiazhuang 050016, china NationalLaboratoryofmolecularOncology CancerInstituteChineseAcademyofMedicalSciences&PekingUnionMedicalCollegeBeijing100021China LifeScienceinstitute HebeiNormalUniversityShijiazhuang050016China

Epidermal-type transglutaminase 3 (TGM3) is involved in the cross-linking of structural proteins to form the cornifiedenvelope in the epidermis. In the present study, we detected the expression of TGM3 in the mouse embryo using ***3 mRNA is weakly presented from E11.5 to E14.5 and increases significantly from E15.5 to birth. Then wedetermined the spatial and temporal expression pattern of TGM3 in the skin and other organs by in situ hybridization. Wefound a deprivation of TGM3 in skin at E11.5, while a rich supply in periderm cells and a weak expression in basal cellsfrom E12.5 to E14.5. From the period of E15.5 to E16.5, after keratinization in the epidermis, TGM3 was expressed inthe granular and cornified layers. The electron microscopic observation of the C57BL/6J mouse limb bud skin develop-ment provided several morphological evidences for the epidermal differentiation. The above findings suggest that theexpression of TGM3 plays a important role in the epidermis differentiation in embryogenesis.

关键词： transglutaminase 3 epidermis differentiation C57BL/6J mouse embryo.

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