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钩藤水提物Batch-2对6-羟多巴胺诱导的SH-SY5Y细胞损伤的神经保护作用(英文)

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生物化学与生物物理进展 2010年第7期37卷 769-778页

作者：石振华 XI Zhao-Wilsor 王浩关鹏于鹏常彦忠段相林赵保路河北师范大学生命科学学院石家庄050016 BioMarker Pharmaceuticals Inc.5941 Optical CourtSan JoseCA 95138USA 北京普尼公司北京100080 中国科学院生物物理研究所北京100101

以前的药理学研究表明,钩藤水溶性提取物C-MED-100TM不仅具有抗氧化活性,而且还具有很好的DNA修复和免疫功能.Batch-2是一种新的水溶性钩藤提取物,而它的自由基清除能力及神经保护作用还未见报道.首先检测了batch-2对六羟多巴胺诱导的SH... 详细信息

以前的药理学研究表明,钩藤水溶性提取物C-MED-100TM不仅具有抗氧化活性,而且还具有很好的DNA修复和免疫功能.Batch-2是一种新的水溶性钩藤提取物,而它的自由基清除能力及神经保护作用还未见报道.首先检测了batch-2对六羟多巴胺诱导的SH-SY5Y细胞损伤的神经保护作用机制,然后利用红外光谱、HPLC和分光光度技术对batch-2的组成成分进行了分析.结果表明,batch-2具有清除各种自由基的能力,尤其是对羟自由基的清除(25mg/L的batch-2对羟自由基的清除率为60%),batch-2可剂量依赖性地抑制6-羟多巴胺诱导的细胞凋亡、脂质过氧化水平、线粒体膜电位的降低和细胞内活性氧和一氧化氮的增加.同时,batch-2抑制了由6-羟多巴胺诱导的SH-SY5Y细胞内iNOS和NF-κB蛋白的上调.结果表明,batch-2对六羟多巴胺诱导的SH-SY5Y细胞损伤的神经保护作用是通过清除活性氧和一氧化氮、抑制iNOS和NF-κB表达实现的.成分分析表明,batch-2中的多酚和奎宁酸含量分别为6.43%和0.0958%.上述结果显示,batch-2的抗氧化机制部分类似于EGCG.对于帕金森病的预防,batch-2是一个潜在具有很好的神经保护作用的天然抗氧化剂.

关键词：绒毛钩藤(Uncaria tomentosa) 抗氧化剂帕金森氏病活性氧 6-羟多巴胺电子顺磁共振

The universality and biological significance of signal molecules with intracellular-extracellular compatible functions

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Chinese Science Bulletin 2000年第19期45卷 1729-1734页

作者： SUN Dayeinstitute of molecular Cell Biology, hebei normal university, shijiazhuang 050016, china institute of molecular Cell Biology Hebei Normal University Shijiazhuang China

Generally, cell signal molecules are classified into the extracellular signal molecules (the first messengers) and the intracellular signal ones (the second messengers). Cyclic adenosine monophosphate (cAMP), calcium ions and calmodulin (CaM) are the traditional intracellular messengers, but they are also present in extracellular matrix (ECM). Some of them have been discovered to act as the first messengers through cell surface receptors. Other second messengers, such as cyclic guanosine monophosphate (cGMP), cyclic adenosine diphosphate ribose (cADPR) and annexin, are also found existing outside animal and plant cells. The existence of these messengers with intracellular-extracellular compatible functions in cells may be a regular biological phenomenon. These compatible messengers might be the communication factors between intracellular and extracellular regions or among the cell populations, and are also important in regulating cell development procedure.

关键词： intracellular signal extracellular signal compatible messenger.

Primary evidence for involvement of IP3 in heat-shock signal transduction in Arabidopsis

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Cell Research 2006年第4期16卷 394-400页

作者： Hong Tao Liu~(1,2,*) Fei Gao~(1,2,*) Shu Juan Cui~2 Jin Long Han~(1,2) Da Ye Sun~2 Ren Gang Zhou~1 ~1 institute of Genetics and Physiology,hebei Academy of Agricultural Sciences,shijiazhuang 050051,china ~2institute of molecular Cell Biology,hebei normal university,shijiazhuang 050016,china institute of Genetics and Physiology Hebei Academy of Agricultural Sciences Shijiazhuang China institute of molecular Cell Biology Hebei Normal University Shijiazhuang China

The role of inositol 1,4,5-trisphosphate （IP3） in transducing heat-shock （HS） signals was examined in *** whole-plant IP3level increased within 1 min of HS at 37℃.After 3 min of HS,the IP3level reached a maximum2.5 fold *** the transgenic Arabidopsis plants that have AtHsp18.2plvmoter-β-glucuronidase （GUS） fusiongene,it was found that the level of GUS activity was up-regulated by the addition of caged IP3at both non-HS and HStemperatures and was down-regulated by the phospholipase C （PLC） inhibitors {1-[6-((17β-3-Methoxyestra-1,3,5（10）-trien-17-yl)amino)hexyl]-2,5-pyrrolidinedione} （U-73122）.The intracellular-free calcium ion concentration ([Ca2+]i) increased during HS at 37℃ in suspension-cultured Arabi-dopsis cells expressing *** with U-73122 prevented the increase of [Ca2+]ito some *** provided primary evidence for the possible involvement of IP3in HS signal transduction in higher plants.

关键词： heat shock signal transduction IP3 PLC [Ca2+]i Arabidopsis

Progress in the participation of Ca2+-calmodulin in heat shock signal transduction

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Progress in Natural Science:Materials International 2009年第10期19卷 1201-1208页

作者： Rengang Zhou a,b,Bing Li c,d,Hongtao Liu a,c,Daye Sun c,d,a institute of Genetics and Physiology,hebei Academy of Agricultural Sciences,shijiazhuang 050051,china b hebei Provincial Key Laboratory of Plant Genetic Engineering,shijiazhuang 050051,china c Academy of Life Sciences,hebei normal university,shijiazhuang 050016,china d hebei Provincial Key Laboratory of molecular and Cell Biology,shijiazhuang 050016,china institute of Genetics and Physiology Hebei Academy of Agricultural Sciences Shijiazhuang 050051 China hebei Provincial Key Laboratory of Plant Genetic Engineering Shijiazhuang 050051 China Academy of Life Sciences Hebei Normal University Shijiazhuang 050016 China hebei Provincial Key Laboratory of molecular and Cell Biology Shijiazhuang 050016 China

A novel heat shock(HS) signal transduction pathway in plants for the participation of Ca2+-calmodulin(CaM) in HS signal trans-duction was *** induces a rapid increase in intracellular free calcium ion levels([Ca2+]i),and the involvement of phospholipase Cinositol 1,4,5-trisphosphate is one of the factors leading to elevation in [Ca2+]i induced by *** also increases the expression of the CaM gene and the accumulation of the CaM *** CaM isoform,AtCaM3,in Arabidopsis is a key member in the HS signal trans-duction ***3 regulates the activity of CaM-binding protein kinase(AtCBK3) or protein phosphatase(AtPP7),promoting the activation of the HS transcription factor,AtHSFA1a,by phosphorylation/dephosphorylation and the expression of heat shock pro-tein genes,then improving heat tolerance in plants.

关键词： Ca2+ Calmodulin Protein kinase Heat shock transcription factor Signal transduction

Synthesis and Stereostructure of New β-Lactam Derivatives of1,5-Benzothiazepines

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Chinese Chemical Letters 1999年第6期10卷 447-448页

作者： Yuan LI Na SUN Sheng JIN(Department of Chemistry hebei university shijiazhuang 050016 institute of Chemistry and molecular Engineering Peking university Beijing 100871) Department of Chemistry Hebei Normal University Shijiazhuang 050016 China Inst. of Chem. and Molec. Eng. Peking University Beijing 100871 China

Reaction of 1,5-benzothiazepine with N-protected glycine gives new a-amino-β-lactamderivatives of 1.5-benzothiazepine. The configuration and conformation of the products wereconfirmed by x-ray diffraction. The resu... 详细信息

关键词： 1.5-Benzothiazepine β-lactam stereospecific reaction

Transglutaminase 3 expression in C57BL/6J mouse embryo epidermis and the correlation with its differentiation

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Cell Research 2005年第2期15卷 105-110页

作者： Jian ZHANG1,2, Hui Ying ZHI1, Fang DING1, Ai Ping LUO1, Zhi Hua LIU1*1National Laboratory of molecular Oncology, Cancer institute, Chinese Academy of Medical Sciences & Peking UnionMedical College, Beijing 100021, china.2Life Science institute, hebei normal university, shijiazhuang 050016, china National Laboratory of molecular Oncology Cancer Institute Chinese Academy of Medical Sciences & Peking Union Medical College Beijing China Life Science institute Hebei Normal University Shijiazhuang China

Epidermal-type transglutaminase 3 (TGM3) is involved in the cross-linking of structural proteins to form the cornifiedenvelope in the epidermis. In the present study, we detected the expression of TGM3 in the mouse embryo using ***3 mRNA is weakly presented from E11.5 to E14.5 and increases significantly from E15.5 to birth. Then wedetermined the spatial and temporal expression pattern of TGM3 in the skin and other organs by in situ hybridization. Wefound a deprivation of TGM3 in skin at E11.5, while a rich supply in periderm cells and a weak expression in basal cellsfrom E12.5 to E14.5. From the period of E15.5 to E16.5, after keratinization in the epidermis, TGM3 was expressed inthe granular and cornified layers. The electron microscopic observation of the C57BL/6J mouse limb bud skin develop-ment provided several morphological evidences for the epidermal differentiation. The above findings suggest that theexpression of TGM3 plays a important role in the epidermis differentiation in embryogenesis.

关键词： transglutaminase 3 epidermis differentiation C57BL/6J mouse embryo.

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植物类整合素蛋白研究进展

生物化学与生物物理进展 2001年第3期28卷 283-286页

作者：孙颖孙大业河北师范大学生命科学学院分子细胞生物学研究室石家庄050016

整合素是动物细胞膜上普遍存在的一类胞外基质受体 ,它所介导的粘附作用参与调节多种细胞功能 .近年来的研究发现在植物细胞中可能也存在类整合素 .综述了在植物类整合素检测、定位、组成、结构、基因以及生理功能研究方面所取得的初步... 详细信息

整合素是动物细胞膜上普遍存在的一类胞外基质受体 ,它所介导的粘附作用参与调节多种细胞功能 .近年来的研究发现在植物细胞中可能也存在类整合素 .综述了在植物类整合素检测、定位、组成、结构、基因以及生理功能研究方面所取得的初步进展与存在的问题 ,并与动物整合素的研究作了比较 .

关键词：类整合素植物质膜生理功能蛋白亚基

拟南芥钙调素定点突变基因分离及其在钙不依赖钙调素结合蛋白检测中的应用(英文)

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生物化学与生物物理进展 2009年第7期36卷 890-896页

作者：高丽王振杰崔素娟河北师范大学分子细胞生物学研究室河北省分子细胞生物学重点实验室

动植物系统研究表明,钙调素不仅在结合钙离子时调节多种靶酶或靶蛋白的活性,而且没有钙离子结合时,还可以通过结合钙不依赖的钙调素结合蛋白,发挥多种生物学作用.然而,目前却没有体内分析钙调素与钙不依赖钙调素结合蛋白相互作用的方法... 详细信息

动植物系统研究表明,钙调素不仅在结合钙离子时调节多种靶酶或靶蛋白的活性,而且没有钙离子结合时,还可以通过结合钙不依赖的钙调素结合蛋白,发挥多种生物学作用.然而,目前却没有体内分析钙调素与钙不依赖钙调素结合蛋白相互作用的方法.首先,采用定点突变的方式,得到了拟南芥钙调素亚型2的多个突变基因mCaM2,随后,大肠杆菌重组表达突变蛋白的电泳迁移率及45Ca2+覆盖分析表明,得到了编码失去钙结合能力的钙调素的突变基因mCaM21234,mCaM21234突变钙调素中所有4个钙结合EF-hand结构域中的关键氨基酸谷氨酸均突变为谷氨酰胺.在酵母双杂交体系中,作为诱饵蛋白的突变钙调素mCaM21234与我们前期体外方法报道的钙不依赖性钙调素结合蛋白AtIQD26存在相互作用.这将为钙不依赖性钙调素结合蛋白提供有用的体内研究工具,有利于我们全面认识钙-钙调素-钙调素结合蛋白信号途径.

关键词：定点突变拟南芥钙调素钙不依赖钙调素结合蛋白

Effects of Intranigral Injection of Glutamate/GABA on Iron Metabolism in the Nigrostriatum of Rats

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Effects of Intranigral Injection of Glutamate/GABA on Iron M...