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Mass Spectrometric Evidence of Heparin Disaccharides for the Catalytic Characterization of A Novel Endolytic Heparinase

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 840-844页

作者： Yapeng CHAO1,3, Shaoxiang XIONG2, Xiulan CHENG1, and Shijun QIAN1* 1Institute of Microbiology, chinese academy of sciences, Beijing 100080, china 2Center of Mass Spectrometry, Institute of Chemistry, chinese academy of sciences, Beijing 100080, china 3graduate school of the chinese academy of sciences, Beijing 100039, china Institute of Microbiology Chinese Academy of SciencesBeijing 100080 China graduate school of the chinese academy of sciencesBeijing 100039 China Center of Mass Spectrometry Institute of Chemistry Chinese Academy of SciencesBeijing 100080 China

Heparinase from different sources can eliminate heparin or/and heparan sulfate into various low-molecular weight heparins with different characteristics. Porcine intestinal mucosa heparin was de- graded into a series of oligosaccharides by a novel heparinase from the species Sphingobacterium. Disaccha- ride components from the digests were separated and purified by ultrafiltration and HPLC. Five major peaks appeared as three types according to their retention time. The mass spectrometry of peak I mainly gave the non-sulfated disaccharide with the mass of 379 Da. Peak II and III were indicated as two major monosulfated disaccharides with molecular mass of 417 and 459 Da respectively. Moreover, the peak III represented an N- acetyl disaccharide. Both peak IV and V showed the same mass of 496 Da, hinting that they were disulfate- substituted disaccharides. No trisulfate-substituted disaccharides were detected in the mixture of the heparin digest though they were abundant in the heparin structure. The results revealed that the heparinase might specifically cut the sites with low sulfated domain in heparin.

关键词： electrospray ionization-mass spectrometry heparin disaccharides heparinase high perfor- mance liquid chromatography Sphingobacterium sp.

Recent progress in fingerprint recognition

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Progress in Natural Science:Materials International 2006年第3期16卷 231-240页

作者： TIAN Jie, CHEN Xinjian, ZHANG Yangyang, YANG Xin, HE Yuliang, LI Liang, XIE Weihua and ZHENG Zhipeng (Center for Biometrics and Security Research, Key Laboratory of Complex Systems and Intelligence Science, Institute of Automation, chinese academy of sciences, Beijing 100080, china graduate school of the chinese academy of sciences, Beijing 100080, china) Center for Biometrics and Security Research Key Laboratory of Complex Systems and Intelligence Science Institute of Automation Chinese Academy of Sciences Beijing 100080 China graduate school of the chinese academy of sciences Beijing 100080 China

Fingerprint recognition has been increasingly used to realize personal identification in civilian' s daily life, such as ID card, fingerprints hard disk and so on. Great improvement has been achieved in the on-line fingerprint sensing technology and automatic fingerprint recognition algorithms. Various fingerprint recognition techniques, including fingerprint acquisition, classification, enhancement and matching, are highly improved. This paper overviews recent advances in fingerprint recognition and summarizes the algorithm proposed for every step with special focuses on the enhancement of low-quality fingerprints and the matching of the distorted fingerprint images. Both issues are believed to be significant and challenging tasks. In addition, we also discuss the common evaluation for the fingerprint recognition algorithm of the Fingerprint Verification Competition 2004 (FVC2004) and the Fingerprint Vendor Technology Evaluation 2003 (FpVTE2003), based on which we could measure the performance of the recognition algorithm objectively and uniformly.

关键词： biometrics fingerprint enhancement matching minutia image registration similarity measures.

IMPLICITIZATION USING UNIVARIATE RESULTANTS

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Journal of Systems Science & Complexity 2010年第4期23卷 804-814页

作者： Liyong SHEN Chunming YUAN school of Mathematical sciences Graduate University Chinese Academy of Sciences Beijing 100049 China Key Laboratory of Mathematics Mechanization Institute of Systems Science Academy of Mathematics and Systems Science Chinese Academy of Sciences Beijing 100190 China

Among several implicitization methods, the method based on resultant computation is a simple and direct one, but it often brings extraneous factors which are difficult to remove. This paper studies a class of rational space curves and rational surfaces by implicitization with univaxiate resultant computations. This method is more efficient than the other algorithms in finding implicit equations for this class of rational curves and surfaces.

关键词： Implicitization rational space curves rational surfaces resultants.

Morphological characteristics of leaf epidermis and size variation of leaf,flower and fruit in different ploidy levels in Buddleja macrostachya (Buddlejaceae)

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Journal of Systematics and Evolution 2009年第3期47卷 231-236页

作者： 1,2Gao CHEN 1Wei-Bang SUN 3Hang SUN 1(Kunming Botanical Garden, Kunming Institute of Botany, chinese academy of sciences, Kunming 650204, china) 2(graduate school of chinese academy of sciences, Beijing 100049, china) 3(Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, chinese academy of sciences, Kunming 650204, china) (Kunming Botanical Garden Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China) (graduate school of chinese academy of sciences Beijing 100049 China) (Key Laboratory of Biodiversity and Biogeography Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China)

Buddleja macrostachya (Buddlejaceae) is a widespread shrub native to the Sino-Himalayan mountains and beyond. It has been found to occur at two ploidy levels, hexaploid, 2n=6x=114 and dodecaploid, 2n=12x=228. To determine if morphological characters might be used as indicators of ploidy levels, we measured floral and fruit length, relative and absolute leaf size, trichome density on both leaf surfaces, and stomatal density and length in different populations of B. macrostachya. In general, flower and fruit length, absolute leaf size, and stomatal length increased with an increase at ploidy level (P0.05) in different populations. Other characters studied such as trichome type, cuticular membrane and ornamentation of stomata, cell and stomatal shape, and anticlinal wall pattern were quite constant in this species. Thus it appears that flower and fruit length, absolute leaf size, and stomatal frequency and length can be used to distinguish hexaploid from dodecaploid cytotypes either in the fleld or in herbarium specimens.

关键词： Buddleja macrostachya dodecaploid hexaploid leaf epidermis ploidy level stomata.

Wind vector retrieval using dual polarization imagery of ASAR

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Progress in Natural Science:Materials International 2006年第11期16卷 1183-1187页

作者： SONG Guiting~ 1,2 , HOU Yijun~1** and QI Peng~1(1. Institute of Oceanology, chinese academy of sciences, Qingdao 266071, china 2. graduate school, chinese academy of sciences, Beijing 100039, china) Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China graduate school Chinese Academy of Sciences Beijing 100039 China

A new algorithm for retrieving wind vector using dual polarization imagery of advanced synthetic aperture radar (ASAR) is developed and tested. Based on the combination of co-polarization and cross-polarization, this new algorithm effectively eliminates the 180° ambiguity which occurs when using single imagery of the ASAR to retrieve wind vector. This algorithm also solves the problem that the retrieval results will break down on very small spatial scales. The results retrieved from dual polarization imagery of ASAR show that the wind speed and direction are in agreement with the data from Quikscat and buoy measurements. The root mean square errors of wind direction and speed between the retrieved results and data from Quikscat are 2.21° and 0.53 m/s, respectively. Comparisons between the new method and the traditional method show that the new methods fit the buoy data and Quikscat data a little better than the traditional method in this image.

关键词： wind vector retrieval ASAR dual polarization.

Preliminary studies on differential defense responses induced during plant communication

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Cell Research 2005年第3期15卷 187-192页

作者： Jin Ying PENG1,3,Zhong Hai LI2,Hui XIANG1,3,Jian Hua HUANG1,3,Shi Hai JIA1,3,Xue Xia MIAO1,Yong Ping HUANG1* 1Institute of Plant Physiology and Ecology,shanghai Institute for Biological sciences,graduate school of the chinese academy of sciences.chinese academy of sciences,300 Fenglin Road,shanghai 200032,china 2chinese National Human Genome Center at shanghai,250 Bibo Road,shanghai 201203.china 3graduate school of the chinese academy of sciences,Beijing 100039,china Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences Graduate School of the Chinese Academy of Sciences Chinese Academy of Sciences Shanghai China graduate school of the chinese academy of sciences Beijing China chinese National Human Genome Center at shanghai Shanghai China

We compared the expression patterns of three representative genes in undamaged tomato and tobacco plants in response to exposure to either tomato or tobacco fed on by Helicoverpa armigera (cotton bollworm). When tomato and tobacco, two species of one family, were incubated in the chambers with the tomato plants damaged by the cotton bollworm, the expression of the PR1, BGL2, and PAL genes was up-regulated in leaves of both plants. However, the levels of gene expression were significantly higher in the tomato than that in the tobacco. In addition, the activities of enzymes, peroxidase, polyphenol oxidase, and lipoxygenase were found to be higher in the tomato than those in the tobacco. Similar results were obtained when the damaged plants were replaced by the tobacco.

关键词： plant-plant communication tomato (Lycopersicon esculentum) tobacco (Nicotiana tabacum) cotton boll- worm (Helicoverpa armigera) inducible defenses.

Suprathermal particle events observed by WIND spacecraft in interplanetary space during 1995―1999 and their classification

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Science china(Technological sciences) 2008年第9期51卷 1542-1553页

作者： SUN LingPeng1,2, WU DeJin1 & WANG DeYu1 1 Purple Mountain Observatory, chinese academy of sciences, Nanjing 210008, china 2 graduate school of chinese academy of sciences, Beijing 100049, china Purple Mountain Observatory Chinese Academy of Sciences Nanjing China graduate school of chinese academy of sciences Beijing China

Fifty-five suprathermal particle events were selected from WIND observations between 1995 and 1999. Based on systematic analysis on the observational characteristics of these events a two-parameter (the rising time and the flux ratio of electrons to protons in each event) classification method was proposed to classify these events. The three clas-sified classes are (1) impulsive electron events with the flux ratio of electrons to protons being bigger than 1 and rising time being shorter than 200 min, (2) impulsive proton events with the flux ratio being smaller than 1 and rising time being shorter than 200 min, and (3) gradual proton events with the flux ratio being smaller than 1 and the rising time being longer than 200 min. In the past, "impulsive solar electron events" were under in-tense research. However, because the selection standards of their velocity dispersions or pitch-angle distributions were inadequate, statistical surveys of selected events were dif-ferent from each other and even some conclusions were not consistent with the theory, for example, the relation of type-III solar radio bursts to the "impulsive solar electron events". The first class of impulsive electron events are associated with type-III radio bursts and with clear velocity dispersions; therefore they ought to originate from the Sun. The second class of the events, which have short continuance time and usually are not associated with type-III radio bursts and without velocity dispersion, are still far away from inter-planetary shocks and most of them do not one-to-one correspond to corrotating interact-ing regions (CIRs); such events are possible results of local interplanetary magnetic field reconnection or electromagnetic disturbances. Finally, about 2/3 gradual proton events of the third class occur with interplanetary shocks, the delay times of which are almost equal to the rising time. Some of these events can be understood as particle accelerations by shocks.

关键词： suprathermal particles solar wind plasma solar eruptive activity interplanetary active regions

A Facile Synthetic Method for (3Z,6Z,9S,10R)-9,10-Epoxy-3,6-heneicosadiene,Sex Pheromone Component of Hyphantria Cunea (Drug)

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chinese Chemical Letters 2005年第4期16卷 468-470页

作者： Chao CHE, Zhong Ning ZHANG StateKey Laboratory IntegratedManagementofInsectsandRodents,Institute of Zoology, chinese academy of sciences, Beijing 100080 graduate school of the chinese academy of sciences, Beijing 100039 chinese Acad Sci State Key Lab Integrated Management Insects & Rod Inst Zool Beijing 100080 Peoples R China chinese Acad Sci Grad Sch Beijing 100039 Peoples R China

Total synthesis of (3Z, 6Z, 9S, 10R)-9, 10-epoxy-3, 6-heneicosadiene, sex pheromone component of Hyphantria cunea (Drug), was achieved using Sharpless AE kinetic resolution and alkylative epoxide rearrangement as ke... 详细信息

关键词： Sex pheromone Hyphantria cunea (Drug) Sharpless kinetic resolution asymmeric synthesis.

Effects of diatoms on reproduction and growth of marine copepods

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Progress in Natural Science:Materials International 2004年第10期14卷 1-7页

作者： ZHANG Guangtao 1,2 and SUN Song 1 (1.Key Laboratory of Marine Ecology&Environment sciences,Institute of Oceanology,chinese academy of sciences,Qingdao266071,china 2.graduate school,chinese academy of sciences,Beijing100039,china) Lab. of Marine Ecology Inst. of Oceanology Chinese Acad. of Sci. Qingdao 266071 China graduate Sch. Chinese Acad. of Sci. Beijing 100039 China

Two hot spots in marine ecology,deleterious effects of diatoms and feeding selectivity of copepods,as well as new progress on these two issues achieved in the recent ten years,are *** two issues are considered correlated *** and their metabolites can induce deleterious effects on growth,reproduction and development of copepods,including increase of mortality and decrease of egg production,hatching and growth *** negative effects,resulting from either chemical toxin or nutritional defi-ciency,can be conquered in natural environments by diverse *** is therefore concluded that deleterious effects of diatoms observed in laboratory or during blooming period are only a special case that accommodation of feeding strategy of copepods is *** under-stand their feeding strategy in natural environments is a prerequisite to explaining the mechanisms of deleterious effects caused by diatoms,and makes it possible to re-evaluate the energy flow in marine ecosystems.

关键词： diatoms copepod feeding strategy reproduction deleterious effects