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Expression of lignin peroxidase H2 from Phanerochaete chrysosporium by multi-copy recombinant Pichia strain

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Journal of Environmental Sciences 2009年第2期21卷 218-222页

作者： WANG Wei,WEN Xianghua department of Environmental Science and Engineering,tsinghua university,beijing 100084,china. department of Environmental Science and Engineering Tsinghua University Beijing 100084 China

The lipH2 gene, encoding the expression of lignin peroxidase, was cloned from Phanerochaete chrysosporium BKM-F-1767 and expressed in Pichia pastoris X-33, a yeast. The cDNA of LiPH2 was generated from total RNA extracted from P chrysosporium by PCR with primers that do not contain a P. chrysosporium lignin peroxidase secretion signal. The gene was then successfully inserted into the expression vector pPICZα, and resulted in the recombinant vector pPICZα-lipH2. The transformation was conducted in two ways. One was using the wild Pichia pastoris as the recipients, which results in the recombinant P. pastoris with single or low lipH2 gene copy. The second was using P. pastoris and single or low lipH2 gene copy as the recipients, which results in the recombinant P. pastoris with multi-copies of lipH2 genes. This study firstly expressed the gene lipH2 in P. pastoris and achieved the successful expression of the lipH2 depending upon the generation of a recombinant strain that contained multiple copies. The lignin peroxidase activity reached a maximum of 15 U/L after 12 h induction.

关键词： heterologous expression lignin peroxidase Pichia pastoris

Molecular architecture and engineering of spider dragline silk protein

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Progress in Natural Science:Materials International 2005年第9期15卷 769-776页

作者： ZHANG Hengmu and LIU Jinyuan(department of Biological Sciences and Biotechnology, tsinghua university, beijing 100084, china) department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Spider dragline silk, which is produced in spider major ampullate gland, is a composite proteinacious fiber with highly repetitive Ala-Gly-rich domain. The unique combination of both high tensile strength and high elasticity makes spider dragline silk superior to almost any other natural or synthetic fibers. Cloning of the genes reveals that the silk is composed of at least two major proteins. Each protein component contains multiple repeats of modular structures that alternate between Ala-rich domains and Gly-rich domains. Molecular engineering not only opens a door to the production of spidroins but also provides a valuable experimental system to test and further establish the relationship between modular structures and mechanical properties. Here, based on our own studies, we review the latest progress of the modular structure and genetic engineering and outline the future prospects.

关键词： spider dragline silk protein modular structure molecular engineering.

A novel extended Kalman filter for a class of nonlinear systems

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Progress in Natural Science:Materials International 2006年第9期16卷 912-918页

作者： DONG Zhe and YOU Zheng (department of Precision Instrument and Mechanology, tsinghua university, beijing 100084, china) department of Precision Instrument and Mechanology Tsinghua University Beijing 100084 China

Estimation of the state variables of nonlinear systems is one of the fundamental and significant problems in control and signal processing. A new extended Kalman filtering approach for a class of nonlinear discrete-time systems in engineering is presented in this paper. In contrast to the celebrated extended Kalman filter (EKF), there is no linearization operation in the design procedure of the filter, and the parameters of the filter are obtained through minimizing a proper upper bound of the mean-square estimation error. Simulation results show that this filter can provide higher estimation precision than that provided by the EKF.

关键词： nonlinear systems state estimation Kalman filtering.

CONSERVATION OF THREE-POINT COMPACT SCHEMES ON SINGLE AND MULTIBLOCK PATCHED GRIDS FOR HYPERBOLIC PROBLEMS

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Journal of Computational Mathematics 2003年第3期21卷 383-400页

作者： Zi-niu Wu (department of Engineering Mechanics, tsinghua university, beijing 100084, china) department of Engineering Mechanics Tsinghua University 北京 100084

For nonlinear hyperbolic problems, conservation of the numerical scheme is important for convergence to the correct weak solutions. In this paper the conservation of the well-known compact scheme up to fourth order of accuracy on a single and uniform grid is studied, and a conservative interface treatment is derived for compact schemes on patched grids. For a pure initial value problem, the compact scheme is shown to be equivalent to a scheme in the usual conservative form. For the case of a mixed initial boundary value problem, the compact scheme is conservative only if the rounding errors are small enough. For a patched grid interface, a conservative interface condition useful for mesh refinement and for parallel computation is derived and its order of local accuracy is analyzed.

关键词： Conservation Compact scheme Uniform grid Multiblock patched grid.

An atlas-based fuzzy connectedness method for automatic tissue classification in brain MRI

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Progress in Natural Science:Materials International 2006年第10期16卷 1106-1110页

作者： ZHOU Yongxin and BAI Jing (department of Biomedical Engineering, tsinghua university, beijing 100084, china) department of Biomedical Engineering Tsinghua University Beijing 100084 China

A framework incorporating a subject-registered atlas into the fuzzy connectedness (FC) method is proposed for the automatic tissue classification of 3D images of brain MRI. The pre-labeled atlas is first registered onto the subject to provide an initial approximate segmentation. The initial segmentation is used to estimate the intensity histograms of gray matter and white matter. Based on the estimated intensity histograms, multiple seed voxels are assigned to each tissue automatically. The normalized intensity histograms are utilized in the FC method as the intensity probability density function (PDF) directly. Relative fuzzy connectedness technique is adopted in the final classification of gray matter and white matter. Experimental results based on the 20 data sets from IBSR are included, as well as comparisons of the performance of our method with that of other published methods. This method is fully automatic and operator-independent. Therefore, it is expected to find wide applications, such as 3D visualization, radiation therapy planning, and medical database construction.

关键词： fuzzy connectedness atlas-based segmentation brain tissue classification MR1.

LARGE DEFLECTION THEORY OF NANOBEAMS

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Acta Mechanica Solida Sinica 2010年第5期23卷 394-399页

作者： Dujuan Zeng Quanshui Zheng 1(department of Engineering Mechanics,tsinghua university,beijing 100084,china) department of Engineering Mechanics Tsinghua University Beijing China

One-dimensional nanostructured materials are often used as beams in many applications such as ultrahigh-frequency resonators and ultrasensitive sensors. Compared with usual macroscopic beams, nanobeams have much higher surface/volume ratios so that their surface energies may play a significant role. Besides, they often bear large deflections due to their typically large slenderness ratios and larger elastic ranges. There is, however, lack of a theory that takes into account of both the above two features owned by nanobeams. In this paper, we present such a theory and give applied examples to show that surface energy and large deflection may individually or jointly have notable effects.

关键词： nanobeam surface energy large deflection

Compactness in fuzzy logic

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Chinese Science Bulletin 1998年第14期43卷 1166-1171页

作者： YING Mingsheng department of Computer Science and Technology, tsinghua university, beijing 100084, china department of Computer Science and Technology Tsinghua University Beijing China

Compactness in Pavelka’s fuzzy logic for some compact lattices of truth values is shown, and the concept of gradual compactness is introduced to establish some corresponding results in a more general setting.

关键词： fuzzy logic compactness gradual compactness.

Semi-solid Processing of Al-7Graghite Composite

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Journal of university of Science and Technology beijing 2001年第3期8卷 203-206页

作者： Peng Zhang, Yunhui Du, Daben Zeng department of Mechanical Engineering, tsinghua university, beijing 100084, china department of Mechanical Engineering Tsinghua University Beijing 100084 China

Al-7graphite composite was processed using Al-7graphite mushy prepared by electromagnetic-mechanical stirring method, and the influence of solid fraction on the distributing of graphite particles in ingot was studied. The results shows that the relationship between solid fraction and stirring temperature of mushy is: f(s) = 591.5-0.897 t (where f(s) is the solid fraction, t is the stirring temperature). For Al-7graphite composite, with the increasing of solid fraction, the aggregation extent of graphite particles reduced gradually, and when solid fraction was larger than 30%, graphite particles could distribute evenly in ingot.

关键词： Al-7graphite composite electromagnetic-mechanical stirring method distributing of graphite particles solid fraction stirring temperature

Study on Numerical Simulation of Mold Filling and Heat Transfer in Die Casting Process

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Journal of Materials Science & Technology 2000年第3期16卷 269-272页

作者： Liangrong JIA, Shoumei XIONG and Baicheng LIU (department of Mechanical Engineering, tsinghua university, beijing 100084, china) department of Mechanical Engineering Tsinghua University Beijing 100084 China

A 3-D mathematical model considering turbulence phenomena has been established based on a computational fluid dynamics technique, so called 3-D SOLA-VOF (Solution Algorithm-Volume of Fluid), to simulate the fluid flow of mold filling process of die casting. In addition, the mathematical model for simulating the heat transfer in die casting process has also been established. The computation program has been developed by the authors with the finite difference method (FDM) recently. As verification, the mold filling process of a S-shaped die casting has been simulated and the simulation results coincide with that of the benchmark test. Finally, as a practical application, the gating design of a motorcycle component was modified by the mold filling simulation and the dies design of another motorcycle component was optimized by the heat transfer simulation. All the optimized designs were verified by the production practice.

关键词： Study on Numerical Simulation of Mold Filling and Heat Transfer in Die Casting Process Mold Simulation