检索结果-南通市图书馆

遗传学进步促进粮食安全与人口健康高峰论坛

作者： Fu Yang1,Ling Zhang1,Xi-song Huo1,Ji-hang Yuan1,Dan Xu1,Sheng-xian Yuan2,Nan Zhu3,Wei-ping Zhou2,Guang-shun Yang3,Yu-zhao Wang1,Jing-li Shang1,Chun-fang Gao4,Feng-rui Zhang5,Fang Wang1,Shu-han Sun1 department of medical Genetics,second military medical university,shanghai,china 2 The Third department of Hepatic Surgery,eastern hepatobiliary hospital,second military medical university,shanghai,china 3 The second department of Hepatic Surgery,eastern hepatobiliary hospital,second military medical university, shanghai,china 4 department of laboratory medicine,eastern hepatobiliary hospital,second military medical university,shanghai,china 5 department of hepatobiliary Surgery,Hebei Cancer hospital,Hebei medical university,Hebei,china.

Hepatocellular carcinoma(HCC) is one of the most common human cancers worldwide, particularly in Southeast Asia and *** than 70-80%of HCC cases occur in high hepatitis B virus(HBV) endemic regions,and 50%of HCC cases worldwide are attributable to chronic infection with ***,the 5-year survival rate of HBV-related HCC patients remains poor,and approximately 600,000 HCC patients die each year despite recent advances in surgical techniques and medical *** previous studies identified many aberrantly expressed protein-coding genes in HCC,novel molecular markers that can help in early diagnosis and risk assessment are still urgently *** is of paramount importance to understand the relationships between clinical symptoms and molecular changes in HCC for developing new diagnosis and treatment strategies for HCC and improving the prognosis of diagnosed patients. The human transcriptome comprises not only large numbers of protein-coding messenger RNAs(mRNAs) but also a large set of non-protein coding transcripts that have structural,regulatory or unknown *** studies of small non-coding RNAs(18 to 200 nucleotides(nt)) have dominated the field of RNA biology in recent years,a surprisingly wide array of cellular functions has also been associated with long non-coding RNAs(IncRNAs).IncRNAs,tentatively defined as non-coding RNAs more than 200 nt in length,are characterized by the complexity and diversity of their sequences and mechanisms of action.A handful of studies have implicated IncRNAs in a variety of disease states,and altered IncRNA levels can result in aberrant expression of gene products that may contribute to cancer ***,there are only preliminary studies on the role of IncRNAs in HCC,and the overall pathophysiological contributions of IncRNAs to HCC remain largely unknown. In this study,we have identified non-overlapping signatures of a small number of IncRNAs that are aberrantly expressed in human HBV

关键词： hepatocellular carcinoma noncoding RNAs PRC2 IncRNAs cell cycle

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Adenovirus-expressed preS2 antibody inhibits hepatitis B virus infection and hepatic carcinogenesis

引用

World Journal of Gastroenterology 2012年第4期18卷 349-355页

作者： Qian Zhang,department of Infectious Diseases,Changhai hospital,second military medical university,shanghai 200433,china Zhi-Qing Li,Hu Liu,Jia-He Yang,department of Hepatic Surgery,eastern hepatobiliary Surgery hospital,second military medical university,shanghai 200438,china Qian Zhang Department of Infectious Diseases Changhai Hospital Second Military Medical University Shanghai 200433 China.

AIM:To investigate the inhibitory effect of hepatitis B virus (HBV) preS2 antibody (preS2Ab) against HBV in-fection and HBV-associated hepatic ***:An adenoviral vector carrying the full-length light and heavy chains of the HBV preS2Ab gene,Ad315-preS2Ab,was *** linked immunosorbent assay (ELISA) and Western blotting analyses were used to determine the preS2Ab expres-sion levels in *** techniques were used to examine the binding affinity between the expressed HBV preS2Ab and HBV-positive liver *** were also used to determine hepatitis B surface antigen (HBsAg) levels to assess the inhibitory effect of the preS2Ab against HBV infection in L02 *** inhibitory effect of preS2Ab against hepatic carcinogen-esis was studied with diethylnitrosamine (DEN)-induced hepatocellular carcinomas (HCCs) in HBV transgenic ***:The expression of HBV preS2Ab increased with increases in the multiplicity of infection (MOI) of Ad315-preS2Ab in L02 cells,with 350.87 ± 17.37 μg/L of preS2Ab when the MOI was 100 plaque forming units (pfu)/*** expressed preS2Abs could recog-nize liver cells from HBV transgenic *** results showed that L02 cells expressing preS2Ab produced less HBsAg after treatment with the serum of HBV pa-tients than parental L02 cells expressing no *** transgenic mice treated with Ad315-preS2Ab had fewer and smaller cancerous nodes after induction with DEN than mice treated with a blank Ad315 vec-tor or untreated ***,the administration of Ad315-preS2Ab could alleviate hepatic cirrhosis and decrease the serum levels of alanine transaminase and aspartate ***:Adenovirus-mediated HBV preS2Ab expression could inhibit HBV infection in L02 cells,and then inhibit DEN-induced hepatocellular carcinogenesis and protect hepatic function in HBV transgenic mice.

关键词： Hepatitis B virus Adenoviral vector PreS2 antibody Hepatocellular carcinoma Gene therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

From cell signaling to cancer therapy

引用

Acta Pharmacologica Sinica 2007年第9期28卷 1494-1498页

作者： Jin DING Yun FENG Hong-yang WANG The International Cooperation laboratory on Signal Transduction,eastern hepatobiliary Surgery Institute,second military medical university,shanghai 200438,china The International Cooperation laboratory on Signal Transduction Eastern Hepatobiliary Surgery Institute Second Military Medical University Shanghai China

Cancer has been seriously threatening the health and life of humans for a *** the intensive effort put into revealing the underlying mechanismsof cancer,the detailled machinery of carcinogenesis is still far from fully *** studies have illustrated that cell signaling is extensively involved intumor initiation,promotion and ***,targeting the key mol-ecules in the oncogenic signaling pathway might be one of the most promisingways to conquer *** targeted drugs,such as imatinib mesylate (Gleevec),herceptin,gefitinib (Iressa),sorafenib (Nexavar) and sunitinib (Sutent),whichevolve from monotarget drug into multitarget ones,have been developed withencouraging effects.

关键词： cell signaling cancer therapy chemotherapy

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Effect of Sirpα1 on the expression of nuclear factor-kappa B in hepatocellular carcinoma

引用

hepatobiliary & Pancreatic Diseases International 2007年第3期6卷 276-283页

作者： Jian-Min Qin,Xing-Wang Wan,Jin-Zhang Zeng and Meng-Chao Wu department of hepatobiliary and Pancreatic Surgery,Beijing Chaoyang hospital,Capital university of medical Sciences,Beijing 100020,china and eastern hepatobiliary Surgery hospital,second military medical university,shanghai 200438, china department of hepatobiliary and Pancreatic Surgery Beijing Chaoyang Hospital Capital University of Medical Sciences Beijing 100020 China. jianminqin@***

BACKGROUND:Signal regulatory protein alpha1(Sirpα1) is a member of Sirps families containing four immunoreceptor tyrosine-based inhibitory motifs(ITIMs) domains in the cytoplasm of and an activated substrate of receptor tyrosine kinase(RTK),that negatively regulates the RTK-dependent cell proliferating signal transduction *** we found that Sirpα1 was closely associated with the occurrence and development of hepatocellular carcinoma(HCC)as well as liver *** it is unclear about the regulatory mechanisms,we established the cell line transfected Sirpα1 gene and preliminarily clarified the mechanisms by which Sirpα1 negatively regulates the carcinogenesis and development of HCC. METHODS:Liver cancer Sk-Hep1 cell was respectively transfected with plasmids of pLXSN,pLXSN-Sirpα1 and pLXSN-Sirpα1Δ4Y 2 ,screened with the drug of G418(1200 μg/ml),and various transfected Sk-Hep1 cell lines were *** protein expressions of P65,P50,IκBα,cyclin D1 and Fas in various Sk-Hep1 cell lines were determined by Western blotting,and P65 and P50 were localized by the immunofluorescence technique. RESULTS:Sirpα1 could significantly upregulate the protein expression of IκBα(*** cell lines,P4Y 2 ,but in nucleus of the Sk-Hep1 cell with wild Sirpα1.P50 protein expression was localized in the cytoplasm and nucleus of the pLXSN Sk-Hep1 cell,but in the nucleus of the Sk-Hep1 cell with wild Sirpα1 and mutant Sirpα1Δ4Y 2 plasmid. CONCLUSIONS:Sirpα1 might negatively regulate and control the abnormal proliferation of liver cancer cells by influencing the protein content and localization of nuclear factor-kappa B,then influence the expression of cyclins such as cyclin D1 in the si

关键词： signal regulatory protein alpha1 carcinoma,hepatocellular nuclear transcription factor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Isoform of Vascular Endothelial Cell Growth Inhibitor (VEGI72-251) Increases Interleukin-2 Production by Activation of T Lymphocytes

引用

Acta Biochimica et Biophysica Sinica 2006年第4期38卷 249-253页

作者： Jing-Juan YAO~1 Min ZHANG~1 Xiao-Hui MIAO~2 Ping ZHAO~1 Shi-Ying ZHU~1 Hui DING~1 Zhong-Tian QI~1 1 Key laboratory for medical Microbiology,PLA,department of Microbiology,second military medical university,shanghai 200433,china 2 department of Infectious Diseases,Changzheng hospital,second military medical university shanghai 200433,china Key laboratory for medical Microbiology PLA Department of Microbiology Second Military Medical UniversityShanghai 200433 China department of Infectious Diseases Changzheng Hospital Second Military Medical UniversityShanghai 200433 China

The objective of this study is to investigate the characteristics of the recombinant variant ofhuman vascular endothelial cell growth inhibitor,VEGI72-251,and compare its biological activities with that ofits prototype VEGI24-174,The recombinant plasmid containing the variant VEGI72-251gene was constructedand expressed in Escherichia *** effects of the expressed VEGI72-251on cell proliferations were checkedin the human umbilical vein endothelial cell line and certain tumor cell lines （ECV304 and B 16）.The inhibitionof VEGI72-251on angiogenesis was detected in the chorioallantoic membrane of chick *** comparisonwith VEGI24-174,the recombinant human VEGI72-251seems to have no effect on the proliferation of endothelialcells and the angiogenesis of the chorioallantoic membrane in *** enzyme-linked immunosorbent assay-based method was used for the measurement of interleukin-2 （IL-2） production by peripheral blood monocytes（PBMCs） treated with *** were pretreated with VEGI72-251（1.25-12.50μg/ml） for 24 h invitro,and the IL-2 concentration in PBMC medium was increased from 354 pg/ml to 1256 pg/*** can beconcluded that VEGI72-251is able to increase the level of human IL-2 production by the activation of *** from VEGI24-174on anti-angiogenesis,VEGI72-251may serve as an anti-cancer factorthrough its activation of T lymphocytes.

关键词： vascular endothelial cell growth inhibitor biological activity interleukin-2

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Tolerance of Mice to Lipopolysaccharide is Correlated with Inhibition of Caspase-3-mediated Apoptosis in Mouse Liver Cells

引用

Acta Biochimica et Biophysica Sinica 2007年第2期39卷 96-100页

作者： Jie LUAN Bingrong ZHOU Hui DING and Zhongtian QI department of Microbiology,State Key laboratory of medical Immunology,second military medical university,shanghai 200433,china department of Microbiology State Key Laboratory of Medical Immunology Second Military Medical UniversityShanghai 200433 China

Bacterial endotoxin lipopolysaccharide (LPS) often results in multiple organ ***,pre-exposure of mice to a sublethal dose of LPS renders the animal tolerant to a lethal dose of *** was designed to determine whether pre-exposure of a small dose of LPS was able to suppressapoptosis in mice when challenged with LPS in combination with D-galactosamine,and to investigate theexpression changes of the apoptosis-associated *** results showed that a characteristic apoptoticDNA fragmentation existed in mouse livers of the LPS-naive group,but not in control groups;and the miceof the LPS-naive group were all dead after 2 ***,in the LPS-tolerance groups,both the lethal rateand apoptotic DNA fragmentation were suppressed after the mice were challenged with LPS/D-galactosamine,and the protection against the lethality and apoptotic reaction could be maintained for up to 7 *** thisperiod, significantly lower levels of caspase-3 and its mRNA appeared in LPS-tolerant groups compared tothose of the LPS-naive group (P<0.05),and the caspase-3 activities gradually recovered as the observationwas *** findings suggest that LPS tolerance could suppress apoptosis in mouse liver cells,andthe expression and activity of caspase-3 could be down-regulated.

关键词： lipopolysaccharide endotoxin tolerance apoptosis caspase-3

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Combined invagination and duct-to-mucosa techniques with modifications:anew method of pancreaticojejunal anastomosis

引用

hepatobiliary & Pancreatic Diseases International 2011年第4期10卷 422-427页

作者： Bin Zhu,Li Geng,You-Gang Ma,Yong-Jie Zhang and Meng-Chao Wu second department of Biliary Surgery and department of Special Treatment,eastern hepatobiliary Surgery hospital,second military medical university,shanghai 200438,china second department of Biliary Surgery Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China department of Special Treatment Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China

BACKGROUND:Soft pancreatic texture and a small main pancreatic duct are thought to be the most significant risk factors for the occurrence of pancreatic fistula (PF),a common and serious complication after pancreaticoduodenectomy (PD).This is in part due to the technical difficulties of pancreaticojejunostomy (PJ) posed by a soft gland with a normal-sized *** deal with this problem,we developed a new anastomotic technique which combines the two most widely used techniques,namely,the invagination technique and the duct-to-mucosa technique,with a modification of the suture route and insertion of a temporary stent ***:Between January 2003 and December 2009,ninetytwo consecutive patients underwent PD in which the new PJ technique was *** and follow-up data of these patients were reviewed for operative details,early postoperative events,and outcomes at 6 months after the *** was defined by the International Study Group on Pancreatic Fistula (ISGPF) guidelines and graded (A,B or C) according to the clinical procedures and ***:In this group of 92 patients,there was only 1 early death from acute renal *** was observed in 11 patients (12.0%),8 in grade A,1 in grade B,and 2 in grade *** the 2 patients in grade C,PF was surgically *** were no early or late deaths attributable to *** months after the operation,all of the patients were free of PJ-related symptoms except for 2,who were found to have ***:Our modified technique is simple and safe in *** data suggest that this technique produces excellent early and medium-term results.

关键词： pancreaticoduodenectomy pancreaticojejunostomy pancreatic fistula

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phosphorylated heat shock protein 27 is involved in enhanced heart tolerance to ischemia in short-term type 1 diabetic rats

引用

Acta Pharmacologica Sinica 2005年第7期26卷 806-812页

作者： Hong CHEN~(2,4,5) Xing-jun WU~2 Xi-yuan LU~3 Liang ZHU~2 Li-ping WANG~2 Huang-tian YANG~3 Hong-zhuan CHEN~(2,6) Wenjun YUAN~(4,6) 2 department of Pharmacology,shanghai second medical university,shanghai 200025 3 laboratory of Molecular Cardiology,Health Science Center,shanghai second medical university,shanghai Institute for Biological Sciences,Chinese Academy of Sciences,shanghai 200025 4 department of Physiology,second military medical university,shanghai 200433,china 5 Now in department of Pharmacology at shanghai second medical university,shanghai 200025,china. department of Pharmacology Shanghai Second Medical University Shanghai China department of Physiology Second Military Medical University Shanghai China laboratory of Molecular Cardiology Health Science Center Shanghai Second Medical University Shanghai Institute for Biological Sciences Chinese Academy of Sciences Shanghai China

Aim:To examine the tolerance of type 1 diabetic hearts to ischemia and *** contents of 27-kDa and 70-kDa heat shock proteins（hsp）aswell as phosphorylated hsp27 were also ***:Hearts from hyper-glycemic rats 3 weeks after streptozocin injection and age-matched normal ratswere subjected to ischemia and reperfusion in *** function and electro-cardiogram were recorded throughout *** heat shock pro-teins were detected with Western ***:Despite depressed systolic func-tion at the baseline,diabetic hearts exhibited considerable enhancement in post-ischemic heart function,manifested by an increase in the maximal rate of leftventricular pressure rise and fall(post-ischemic dp/dtmaxand dp/dtminwere 560±117and-313±68 mmHg/s in control,n=7,1249±57 and-1204±36 mmHg/s in diabetes,n=10,P<0.01).Reperfusion ventricular fibrillation in the diabetic group were at-tenuated compared with controls（1.5±0.3 vs 7.2±2.1 min in control,P<0.01）.Theincreased heart resistance to ischemia in diabetes was associated with hypergly-cemia and accompanied by enhanced expression of myocardial phosphorylatedhsp27 with normal aortic vessel *** was abrogated bymetabolic correction with insulin and accompanied by phospho-hsp27 ***:Heart resistance to ischemia is increased in type 1 diabetes,andhyperglycemia may present a mild yet stressful stimulus leading to upregulationof endogenous stress protein,which may play a potential role in cardioprotectionand compensate for detrimental effects of hyperglycemia in diabetes.

关键词： type 1 diabetes mellitus ischemia reperfusion heart heat shock proteins

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The expression and antigenicity identification of recombinant rat TGF-β1 in bacteria

引用

Cell Research 2001年第2期11卷 95-100页

作者： GAO CHUN FANG, XIAN TAO KONG, AXEL M GRESSNER, RALF WEISKIRCHEN department of laboratory medicine, Changzheng hospital, second military medical university, shanghai 200003, china Institute of Clinical Chemistry and Pathobiochemistry, Central laboratory, R department of laboratory medicine Changzheng Hospital Second Military Medical University Shanghai China Institute of Clinical Chemistry and Pathobiochemistry Central Laboratory RWTH-University Hospital Aachen Germany

In order to study structure-function details of TGF-β1, the recombinant mature form of rat TGF-β1 was expressed in bacteria. Synthesis of the 112 amino-acid carboxyl-terminal part of TGF-β1 (amino acid 279-390) was controlled by an inducible gene expression system based on bacteriophage T7 RNA polymerase. This system allowed an active and selective synthesis of recombinant TGF-β1. The molecular weight of expressed TGF-β1 monomer determined on SDS-polyacrylamide gel under reducing conditions was about 13 kD. Serial detergent washes combined with a single gel-filtration purification step were sufficient to purify the expression product to homogeneity. Amino-terminal sequencing revealed that the N-terminal of the recombinant protein was identical to the published data. In Western blot analysis the recombinant polypeptide showed excellent antigenicity against polyclonal TGF-β1 antibody. The mature recombinant rat TGF-β1 expressed in this study provides a useful tool for future detailed structural and functional studies.

关键词： TGF-β1 recombinant expression inclusion bodies purification antigenicity.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Changes of serum alpha-fetoprotein and alpha-fetoprotein-L3 after hepatectomy for hepatocellular carcinoma: prognostic significance

引用

hepatobiliary & Pancreatic Diseases International 2012年第6期11卷 618-623页

作者： Xiao-Feng Zhang, Zheng-Feng Yin, Kui Wang, Zong-Qin Zhang, Hai-Hua Qian,Le-Hua ShiAuthor Affiliations: The 4th department of Hepatic Surgery ,Molecular Oncology laboratory , eastern hepatobiliary Surgery hospital, second military medical university, shanghai 200438, china The 4th department of Hepatic Surgery Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China Molecular Oncology laboratory Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China

BACKGROUND: Alpha-fetoprotein (AFP) is the most established tumor marker of hepatocellular carcinoma (HCC), but one of its limitations is non-specificity. Many studies have demonstrated that alpha-fetoprotein-L3 (AFP-L3) is more specific than AFP in the early diagnosis and prognosis of HCC. However, there is a lack of knowledge about the post-hepatectomy profiles of serum AFP and AFP-L3 values in HCC patients. To identify the profiles after surgical resection of HCC, we analyzed the correlation between the profiles and postoperative HCC recurrence or survival, and evaluated their utility in predicting postoperative therapeutic efficacy and prognosis. METHODS: From August 2003 to December 2004, 318 patients with positive serum AFP who had received surgical resections were enrolled in this study. Preoperative and postoperative serum AFP and AFP-L3 levels were measured simultaneously and regularly, and their postoperative profiles during a long term follow-up were recorded and summarized. RESULTS: A high ratio of AFP-L3 to total AFP was shown to correlate with pathologic features of aggressiveness. The overall 1-, 3-, and 5-year recurrence rates of the whole series were 28% 57%, and 84%, and the overall survival rates were 86%, 61% and 33%, respectively. The changes of serum AFP and AFP-L3 after hepatectomy for HCC were classified into 3 groups (group A: AFP-L3 undetectable; group B: AFP-L3 10%). Patients with positive postoperative AFP-L3had significantly earlier recurrence than those with negative results. The overall survival was significantly shorter in the positive groups than in the groups negative for postoperative ***: Post-hepatectomy changes in serum AFP and AFP-L3 levels occurred in three distinct patterns, which were closely correlated with HCC recurrence and patient survival with different prognostic values.

关键词： hepatocellular carcinoma hepatectomy alpha-fetoprotein alpha-fetoprotein-L3

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：