检索结果-南通市图书馆

Identification of Prorocentrum minimum and Takayama pulchella by fluorescence in situ hybridization through epifluorescence microscopy and flow cytometry

引用

Acta Oceanologica Sinica 2009年第2期28卷 103-114页

作者： HOU Jianjun LAI Hongyan HUANG Bangqin CHEN Jixin College of Fisheries Huazhong Agricultural University Wuhan 430070 China College of Life Sciences Hubei Normal University Huangshi 435002 China State Key Laboratory of Marine Environmental Science Environmental Science Research Center Xiamen University Xiamen 361005 China Key Laboratory of Freshwater Fish Germplasm Resources and Biotechnology Yangtse River Fisheries Research Institute Jinzhou 434000 China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China

Partial rDNA sequences of Prorocentrum minimum and Takayama pulchella were amplified, cloned and sequenced, and these sequence data were deposited in the GenBank. Eight oligonucleotide probes （DNA probes） were designed based on the sequence analysis. The probes were employed to detect and identify P. minimum and T. pulchella in unialgal and mixed algal samples with a fluorescence in situ hybridization method using flow cytometry. Epifluorescence micrographs showed that these specific probes labeled with fluorescein isothiocyanate entered the algal cells and bound to target sequences, and the fluorescence signal resulting from whole-cell hybridization varied from probe to probe. These DNA probes and the hybridization protocol we developed were specific and effective for P. minimum and T. pulchella, without any specific binding to other algal species. The hybridization efficiency of different probes specific to P. minimum was in the order： PM18S02 PM28S02 〉 PM28S01 〉PM18S01, and that of the probes specific to T. pulcheUa was TP18S02 TP28S01 〉 TP28S02 〉TP18S01. The different hybridization efficiency of the DNA probes could also be shown in the fluorescent signals between the labeled and unlabeled cells demonstrated using flow cytometry. The DNA probes PM18S02, PM28S02, TP18S02 and TP28S01, and the protocol, were also useful for the detection of Mgae in natural samples.

关键词： oligonucleotide DNA probes Prorocentrum minimum Takayama pulcheUa fluorescence in situ hybridization flow cytometry

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The point of no return and pectoral angle of Japanese anchovy(Engraulis japonicus) larvae during growth and starvation

引用

Acta Oceanologica Sinica 2007年第5期26卷 144-152页

作者： WAN Ruijing LI Xiansen ZHUANG Zhimeng JOHANNESSEN Arne Key Laboratory of Ministry of Agriculture for Sustainable Utilization of Marine fishery Resources Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China Department of Biology University of Bergen Bergen Norway

At a temperature of 23.0 - 24.8℃, the mixed feeding of Japanese anchovy larvae was initiated 24 h before the yolk-sac was exhausted. The point of no return （PNR） was reached on the 6th day after hatching. On the 4th day after hatching, the pectoral angle appeared in both fed and unfed anchovy larvae although it was more evident and sharper in the starved and the PNR stage larvae than in the fed ones. According to observations of larvae collected in the sea, the pectoral angles were evident not only in the larvae of 3.62 - 7.44 mm in standard length, but also in the larvae of 2.70 mm in standard length with remnants of yolk. The pectoral angles became diffuse when the larvae reached 7.84 mm and vanished at 9.86 mm. The pectoral angle cannot be used as a criterion to distinguish healthy from starving larvae.

关键词： Engraulis japonicus larvae starvation point of no return (PNR) pectoral angle

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Relationship　between　deep－sea　fish　distributions　and　oceanic　conditions　of　the　East　china　Sea

引用

Acta Oceanologica Sinica 1994年第4期13卷 535-550页

作者： Shen Jin’ao ( Department of fishery Engineering, Shanghai Fisheries University, 334 Jungong Road, Shanghai 200090, china)

Based on the data collected from the exploratory trawl fishing and environmental surveys in the western deep sea of the East china Sea in 1980￣1981, this paper shows the relationship between deepsea fish distributions and oceanic conditions. The bottom environment is chiefly controlled by the Kuroshio and its branches. Generally, the temperature and salinity are high in the northwest and low in the southeast of the survey waters, though their seasonal variations are very small. The divepsity and stability of oceanic conditions lead to the complexity of the fish species and their distributions,and the distribution features have a close relationship with the vertical structure of the Kuroshio. In the case of different depths near the bottom, not only most of the fish species distributions are different but also the fish resource densities vary considerably. The concentrated areas of major spotes, such as yellow-fin filefish (Navodon xanthopterus) and deep-sea smelt (Glossanodon semifasciata)etc., spread in the upwelling waters from the Kuroshio with more abundant zooplankton. The new fishing ground of yellow-fin file fish has been exploited in recent years and other fishing grounds will be available there.

关键词： deep between East china

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Satellite SAR observation of shallow bottom topography of the east Australia Sea

引用

Acta Oceanologica Sinica 1999年第2期18卷 215-223页

作者： Zhou Changbao G. Cresswell P. Tildesley and C. S. Nilsson 1.Second institute of oceanography, State Oceanic Administration, Hangzhou 310012, china 2. CSIRO Division of oceanography, Hobart, Tasmania 7001, Australia (Received June 9, 1997 accepted August Second institute of oceanography State Oceanic Administration Hangzhou 310012 China CSIRO Division of oceanography Hobart Tasmania 7001 Australia

A preliminary account of synthetic aperture radar (SAR) imaging mechanisms of oceanic bottom topographic features and a description of the principles of the information extracted from SAR images and the processing and the analysis technology of SAR images are made. Some results were obtained from researching shallow topography and its bathymetric features of the regions of offshore in the east Australia Sea by ERS-1 SAR images. The preliminary research indicates that these features covered with SAR have never been reported before and some results of them are yielded for the first time in Australia. This information is very valuable in a great number of applications to oceanic engineering, shipping navigation, marine fishery and environment, as well as oceanographic research and development. The paper comes to the conclusion that SAR remote sensing of oceanography and its applications are of great potential.

关键词： Satellite SAR observation shallow topography china-Australia Science and Technology Cooperative Program.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

浙江北部沿海婆罗囊螺的生物学特征

引用

水产学报 2003年第4期27卷 343-349页

作者：王志铮张义浩李太武李仁伟孙爱丽浙江海洋学院渔业学院浙江海洋学院科研处宁波大学生命科学与生物工程学院舟山市水产养殖技术研究开发中心宁波大学生命科学与生物工程学院浙江舟山　316004 浙江宁波　315040 浙江舟山　316000

根据2001年5月至2002年5月间浙江北部沿海逐月采集所得1146颗婆罗囊螺生物学测定资料,并结合该螺野外生物学观察资料进行生长生物学综合分析。结果表明,该螺为软泥相潮间带数量优势种,垂直分布差异显著。喜阳,常出没于较干燥的海涂表面... 详细信息

根据2001年5月至2002年5月间浙江北部沿海逐月采集所得1146颗婆罗囊螺生物学测定资料,并结合该螺野外生物学观察资料进行生长生物学综合分析。结果表明,该螺为软泥相潮间带数量优势种,垂直分布差异显著。喜阳,常出没于较干燥的海涂表面,栖息地受潮汐、光照和饵料分布调控;属明显集群繁殖类型,亲体产后大多死亡,一年生;5-9月为繁殖期,以6-7月为盛,6月下旬开始有幼螺群体出现,其生长速率在当年7-9月和次年4-6月各有一个高峰,11月-次年2月基本停止生长,肥满度以次年6月初为最高;壳高(H)与壳宽(B)成线性相关,其方程为B=0.3224+0.4916H(r=0.9770),壳高与鲜重、干重呈幂函数相关,其回归关系方程分别为W湿=2.1×10-4L2.8352(r=0.8448)、W干=2.97×10-4L2.2803(r=0.8939);最后,还就杀灭婆罗囊螺的时机进行了探讨。

关键词：浙江北部沿海婆罗囊螺生长特征生物学

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Estimation of genetic parameters and breeding values in shrimp F enneropenaeus chinensis using the REML/BLUP procedure

引用

Acta Oceanologica Sinica 2011年第1期30卷 78-86页

作者： ZHANG Tianshi KONG Jie LUAN Sheng WANG Qingyin LUO Kun TIAN Yia College of Life Sciences and Technology Ocean University of China Qingdao 266003 China Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Ministry ofAgriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071 China institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China

An analysis of a selection experiment was used to assess the impact of various animal model struc- tures on REML estimates of variance components. The analyses were carried out based on 162 d body mass （BM） of 1 287 animals from 21 paternal half-sib groups of Fenneropenaeus chinensis. Estimated breeding values （EBV） of BM of all individuals were estimated using eight statistical models （A, AB, ABC, ABDC, ABMFC, ABMDC, ABFDC and ABMFDC） and BLUP （best linear unbiased prediction）. These models were designed involving factors such as sex, spawn date as fixed effects, maternal genetic effects, full-sib family effects as random effects, mean BM of families at tagging and age at recording （covariate）. The results demonstrate the importance of correct interpretation of effects in the data set, particularly those that can influence resemblance between relatives. The data structure and the particular model that was applied markedly influenced the magnitude of variance component estimates. Models based on few effects obtained upward biased estimates of additive genetic variance. The accuracy of genetic parameters and breeding value es- timated by ABFDC model was higher than other models. The results imply that additive genetic direct value, full-sib family effects, and covariance effects besides sex and spawn date as fixed effects were very important for estimating genetic parameters and breeding value of body mass. This model had a heritability estimate of 162 d BM of 0.44. The comparison of the efficiency of selection based on breeding values or phenotypic value revealed great difference： average breeding value of the best 24 families selected by the 162 d BM breeding value and phenotype were 0.577 g and 0.366 g, respectively, representing a 36.57% higher efficiency in the former. In conclusion, selection based on breeding value was more effective than selection based on phenotypic value. Our results indicate that effects influencing the magnitude of estimates should be tak

关键词： Fenneropenaeus chinensis animal model BLUP breeding value

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

舟山渔场及其邻近海域腹泻性贝类毒素的初步研究

引用

水产学报 2002年第6期26卷 528-532页

作者：袁骐中国水产科学研究院东海水产研究所上海　200090

在江苏及浙江共5个地点采集了8种贝类,使用四甲基氢氧化胺和9-氯乙基蒽衍生的高效液相色谱法,检测样品的大田软海绵酸含量。结果表明:样品中的大田软海绵酸含量从15.75～218.95ng·g-1,平均含量为37.10ng·g-1,检出率为48%。有一个样品... 详细信息

在江苏及浙江共5个地点采集了8种贝类,使用四甲基氢氧化胺和9-氯乙基蒽衍生的高效液相色谱法,检测样品的大田软海绵酸含量。结果表明:样品中的大田软海绵酸含量从15.75～218.95ng·g-1,平均含量为37.10ng·g-1,检出率为48%。有一个样品的含量超过食用标准(200ng·g-1)。从该结果与其他研究结果可以推断腹泻性贝类毒素在我国分布区域较广,且在某些地区含量较高。

关键词：腹泻性贝类毒素四甲基氢氧化胺 9-氯乙基蒽高效液相色谱法

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

THE RELATIONSHIP BETWEEN SHRIMP RESOURCES IN THE HUANGHAI AND BOHAI SEAS AND SEA SURFACE TEMPERATURE ABNORMALITY

引用

Acta Oceanologica Sinica 1985年第4期 548-556页

作者：李若钝 institute of oceanography National Bureau of oceanography Qingdao

In this paper the relationship between the variation of shrimp resources （which is sensitive to the sea temperature of the Huanghai and Bohai Seas） and the equatorial sea surface temperature is analysed, the results obtained showing high correlation between the two. Therefore, a new approach to studying long-term variational law of fishery resources and forecasting method is made through large-scale air-sea interaction.

关键词： SHRIMP THE RELATIONSHIP BETWEEN SHRIMP RESOURCES IN THE HUANGHAI AND BOHAI SEAS AND SEA SURFACE TEMPERATURE ABNORMALITY SST

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Molecular cloning,characterization and expression analysis of Sox9a and Foxl2 genes in half-smooth tongue sole(Cynoglossussemilaevis)

引用

Acta Oceanologica Sinica 2011年第1期30卷 68-77页

作者： DONG Xiaoli CHEN Songlin JI Xiangshan SHAO Changwei College of Fisheries Ocean University of China Qingdao 266003 China Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry ofAgriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071 China College of Animal Science and Technology Shandong Agricultural University Taian 271018 China

Two transcription factors, Sox9a and Foxl2 were cloned from half-smooth tongue sole （Cynoglossus semilaevis）. Sox9a is a new duplication of *** Sox9 gene. The complete cDNA of Sox9a gene was 1 842 bp long coding for 487 amino acids and Foxl2 gene was 1 817 bp coding for 308 amino acids. Sox9a was expressed higher in male brain, pituitary and gonad and Foxl2 were higher in female brain, pituitary and gonad. The expression of Sox9a gene in gonads of neo-males was higher than that of normal females. Sox9a and Foxl2 were expressed higher in gastrula stage than in other stages. In the period of sex differentiation, the expression of Sox9a was first going up and then going down and Foxl2 was higher expressed at 37 dph. The highest expressions of Sox9a and Foxl2 genes occurred in nine-month and 12-month old gonad tissues, respectively. Sox9a gene was considered to have inevitable links with sex reversal, sex differentiation and cell differentiation of embryos and formation of spermatogenic ceils. Foxl2 was considered to play a role in sex differentiation, cell differentiation of embryos but not to be necessary for sex determination and sex reversal.

关键词： half-smooth tongue sole Cynoglossus semilaevis Sox9 Foxl2 real-time PCR sexreversal neo-male

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

超强纤维材料的试验研究及其在渔业中的应用前景

引用

水产学报 2000年第5期24卷 480-484页

作者：王鲁民中国水产科学研究院东海水产研究所!上海200090

After 20 years of systematic studies,15 subfamilies,38 genus and 104 species of braconid wasp in Hunan province are *** to the studies on both male and femal genitalia of Aridelus hunanensis You,Xiong et Zhou and Aridelus rutilipes Papp,and on both male genitalia of Streblocera flava You et Xiong and Streblocera okadai Watanabe,it is pointed in this paper that Aridelus hunanensis You,Xiong et Zhou and Streblocera flava You et Xiong are still considered valid independent *** disagrees with Chen X.’s and *** Achterberg’s (1997) opinion that Aridelus hunanensis You,Xiong et Zhou is a junior synonym of Aridelus rutilipes Papp,and Streblocera flava You et Xiong is the same species of Streblocera okadai Watanabe. Key words:Braconid wasp;taxonomy;fauna;Hunan

关键词：超强纤维强力渔业

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：