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Identification of the proteome composition occurring during the course of embryonic development of bees（Apis mellifera.）selected for high royal jelly production

Identification of the proteome composition occurring during ...

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中国养蜂学会生物学专业委员会2007年会

作者： Jianke Li~(a*) Lan Zhang~a Zhaohui Zhang~b Yinghong Pan~b ~aInstitute of Apicultural Research,Chinese Academy of Agricultural Science,beijing 100093,china ~bInstitute of crop Science,Chinese Academy of Agricultural Sciences,The national key facility for crop gene resources and genetic improvement,12 Zhongguancun Nandajie,beijing 100081,china

＜正＞To investigate the composition and function of the proteome during embryonic development of worker bees from a strain of Apis mellifera artificially selected for increased royal jelly yield（Apis mellifera L.）,proteins were partially identified by two-dimensional gel electrophoresis,mass spectrometry and protein engine identification tools that were applied to the honeybee *** of 38 high abundance proteins selected for MS fingerprinting,20 could be identified as representing annotated genes of the honey bee and 1 could be encoded by Strongylocentrotus purpuratus genome. These including 6 proteins related to the metabolism of carbohydrates and energy production,6 proteins belonged to the heat shock protein family,3 proteins related to cytoskeletal proteins,4 proteins related to the antioxidant system of the embryo and 2 proteins related to growth regulation of the embryo,*** results suggest that embryogenesis is a dynamic and integrated process that requires a variety of proteins as it unfolds.

关键词： high royal jelly producing bees(Apis mellifera L.) egg of the worker bee peptide mass fingerprint proteome MALDI-TOF

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QTL for grain yield and yield parameters in winter wheat (Triticum aestivum L.)

QTL for grain yield and yield parameters in winter wheat (Tr...

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中国作物学会2007年全国作物遗传育种学术研讨会

作者： LIN Hai GUO Hui-jun ZHANG Xiu-ying WANG Rui-xia XIAO Shi-he~* (The national key facility for crop gene resources and genetic improvement,Institute of crop Science,12 Zhongguancun South Street,beijing,100081,P.R)

Field trials with a set of 108 doubled haploid lines(DHs)derived from a cross between the Chinese winter wheat ***9613 and H1488 were run at beijing(china).Phenotypic data were recorded for major agronomic yield traits,*** weight per ear,grain number per ear and thousand grain weight(Tgw)in two field trials at beijing. Based on the phenotypic data and a genetic map comprising 168 SSR markers,an analysis of quantitative trait loci (QTL)was carried out for yield and yield parameters using the composite interval mapping(CIM)approach.A total of 14 QTL were detected for these traits across two *** of these QTL located on chromosomes 1A and 2B, respectively,exhibited pleiotropic *** showing pleiotropic effects will be very useful for understanding the homeologous relationships of QTL and designing an appropriate marker-assisted selection programme by multi-trait selection in order to accumulate(“pyramide”)favorable alleles at different loci.

关键词： Doubled haploids Quantitative trait loci(QTL) Grain yield wheat(Triticum aestivum L.)

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Identification of the proteome complement of hypopharyngeal glands from two strains of honeybees（Apis mellifera）

Identification of the proteome complement of hypopharyngeal ...

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中国养蜂学会蜜蜂饲养管理专业委员会第十三次学术研讨会

作者： Jianke Li~(a,*)Mao Fenga Zhaohui Zhang~b Yinghong Pan~b a Institute of Apicultural Research,Chinese Academy of Agricultural Science,beijing 100093,china b Institute of crop Science,Chinese Academy of Agricultural Sciences,The national key facility for crop gene resources and genetic improvement,12 Zhongguancun Nandajie,beijing 100081,china

＜正＞The protein complement of the hypopharyngeal gland（HG）of winter worker bees from a strain of Apis mellifera artificially selected for increased royal jelly yield and Carnica winter worker bees was *** were partially identified using two-dimensional gel electrophoresis（2-DE）.MALDI-TOF MS and protein engine identification tools that were utilized for the honeybee *** identified proteins in the two bees strains were assigned to major royal jelly（RJ）proteins（MRJPs）.Marked differences were found in the heterogeneity of the MRJPs,in particular *** of the proteins,α-glucosidase and glucose oxidase,were related to carbohydrate metabolism and *** the first time in the HG of honeybees,two proteins, peroxiredoxin and thioredoxin peroxidase,which are related to antioxidation functions,and actin 5C,a major cytoskeletal actin protein which may supply enough actin for normal function of cells, have been *** suggest that the HGs serve a storage function in winter and that during winter the period the HG of high RJ producing bees store more proteins than those of Camica bees.

关键词： Apis mellifera winter bees hypopharyngeal gland proteome

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genetic ANALYIS OF SALT TOLERANCE IN A RIL POPULATION OF WHEAT （TRITICUM AESTIVUM L.）

GENETIC ANALYIS OF SALT TOLERANCE IN A RIL POPULATION OF WHE...

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全国作物生物技术与诱变技术学术研讨会

作者： MA Liqing~(1,2) ZHOU Erfeng~(1,2) ZHOU Ronghua~2, WANG Guoying~1 and JIA Jizeng~(2*) (1 College of Biotechnology, china Agricultural University, beijing 100094, china 2 key laboratory of crop germplasm and biology, MOA, national key facility for crop gene resources and genetic improvement, Institute of crop Germptasm Resouces, CAAS, beijing 100081, P. R. china)

＜正＞ A population of 114 RILs, derived from the cross Opata85 x W7984, was used to genetically analyse the response of wheat to salt stress. This analysis resulted in the identification of 69 QTL mapping to all wheat chromosomes except 1B, 1D and 7D. Of these QTL, 22 were effective during the germination stage, and 47 at the seedling stage. Many of the traits related to salt tolerance mapped to common chromosome intervals, such as Xglk683-Xcdo460 on chromosome 3A, Xfob168-Xbcd147 on chromosome 3B, Xcko1081-Xfbb226 on chromosome 4DL and Xpsr106-Xfbb283 on chromosome 6DL. QTL located in the interval Xcko1081-Xfbb226 （chromosome 4DL） were effective during the germination stage, while those in the interval Xpsr106-Xfbb283 （chromosome 6DL） were relevant to the seedling stage. The QTL in the intervals Xglk683-Xcdo460 （chromosome 3AS） and Xfbb168-Xbcd147 （chromosome 3BL） were effective at both the germination and the seedling stage.

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FLOWING AND MAPPING OF GLYPHOSATE-RESISTANT gene IN SOYBEAN

FLOWING AND MAPPING OF GLYPHOSATE-RESISTANT GENE IN SOYBEAN

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全国作物生物技术与诱变技术学术研讨会

作者： ZHOU Bo, GUAN Rongxia, TAO Bo, CHANG Ruzhen, LIU Zhangxiong, QIU Lijuan (national key facility of crop gene resources and genetic improvement, Chinese Academy of Agricultural Sciences, 100081, beijing Lab of Weed and Pesticide, Northeast Agriculture University, Haerbin, 150030)

＜正＞ In order to evaluate the glyphosate-resistant gene flowing in different soybean varieties, the glyphosate-resistant soybean（D001） with purple stem and purple flower were planted in the center of field. Around the center in eight directions, three non-glyphosate-resistant soybean with green stem and white flower was planted at the distance 5m, 7m, 10m, 14m, 19m and 25m respectively, including a soybean cultivar zhonghuang15, semi-wild soybean YDD722 and a wild soybeanaccession. The seeds of the three non-transgenic soybean were harvested and then planted for the examination of gene flowing. According to stem color, purple stem seedlings were selected as heterozygote. The wild soybean accession had lowest heterozygote, of which the heterozygous rate is 0 in 64740 individual plants;following with the heterozygous rate of zhonghuang15 foe 1.35% of 40415 ones and YDD722 for 0.05% of 63374 ones. No significant difference of heterozygous rate was observed for the three types of soybeans in different distances or directions by means of variance. And the rate decreased by the increasing of distance, such as 0.58% in 5m and 0.07% in 25m. So the glyphoate-resistant gene of soybean can flow to the non glyphosate-resistant soybean by natural pollination.

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Decoding the rice functional genome:progress and implications for crop genetic improvement

Decoding the rice functional genome:progress and implication...

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2011全国植物生物学研讨会

作者： Qifa Zhang national key Laboratory of crop genetic improvement and national Center of Plant gene Research, Huazhong Agricultural University,Wuhan 430070,china

Tremendous progress has been made in rice functional genomic research in the last decade with the long-term goal to understand the functional genome of *** the availability of high quality rice genome sequence,there has been rapid accumulation of functional genomic resources,including:large mutant libraries by T-DNA insertion,transposon tagging,and chemical mutagenesis;global expression profiles of the genes in the entire life cycle of rice growth and development;full-length cDNAs for both indica and japonica rice;sequences from resequencing large numbers of diverse germplasm *** resource development has greatlv accelerated gene *** the end of 2010,over 600 genes had been cloned

关键词： Oryza sativa functional genomics gene cloning Rice 2020 breeding application

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SOYBEAN （GLYCINE MAXL.） CORE COLLECTIONS IN china: ITS ESTABLISHMENT AND APPLICATION REVEALED BY BOTH AGRONOMIC CHARACTERS AND SSR MARKERS

SOYBEAN （GLYCINE MAXL.） CORE COLLECTIONS IN CHINA: ITS EST...

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全国作物生物技术与诱变技术学术研讨会

作者： QIU Lijuan~(1**), GUAN Rongxia, LIU Zhangxiong, LI Yinghui and CHANG Ruzhen (national key facility of crop gene resources and genetic improvement, Institute of crop Science, Chinese Academy of Agricultural Sciences)

＜正＞ There are over 23 thousands soybean accessions in china, where has the most soybean germplasm in the world. It becomes urgent task to evaluate and utilize the collection efficiently. Based on the passport data and agronomic traits of these germplasm, a total of 20 sampling methods were compared and the best one was subjected to collection samples. The primary core collection was consisted of the samples selecting by the best method and artificially adding few accessions with traits that not included. Then this collection was analyzed by a core set of SSR loci, which identified with 80 autumn soybean accessions and confirmed with 190 random soybean accessions from the whole country. The core collection established with both SSR data

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Evaluation and Quantitative Inheritance of Several Drought-Relative Traits in Maize

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Journal of Integrative Agriculture 2008年第3期7卷 280-290页

作者： FU Feng-ling FENG Zhi-lei GAO Shi-bing ZHOU Shu-feng LI Wan-chen key Laboratory of crop genetic resources and improvement,Ministry of Education/Maize Research Institute.Sichuan Agricultural University,Ya’an 625014,P.R.china key Laboratory of crop genetic resources and improvement Ministry of Education/Maize Research Institute Sichuan Agricultural University Ya'an 625014 P.R. China

The authors evaluated 57 parental inbred lines of maize hybrids disseminated in Southwest china for drought tolerance under drought-stressed and well-watered *** regression analyses between drought tolerant coefficients of the grain yield per plant and 15 morphological and physiological traits measured from a subset of 12 selected lines, identified traits 1 and 5,which were important for drought tolerance,at the seedling and reproductive stages respectively. gene effects,combining abilities,and heritabilities of these traits were estimated using generation mean and diallel cross *** effect was more important than additive effect for the plant height,anthesis-silking interval(ASI), root weight,and the grain yield per plant,whereas,they were about equal for the leaf emergence *** variances of special combining ability(SCA)were about double that of the general combining ability(GCA)for plant height,ASI and grain yield per plant,although they were about equal for leaf emergence rate and root *** sense beritabilities of the five traits for the reproductive stage were not high(12.8-29.6%),although broad sense heritabilities for plant height, ASI,and grain yield were as high as 70-85%.A segregating population consisting of 183 Fplants from the cross N87-1 (drought tolerant)×9526(susceptible),was genotyped at 103 SSR loci and the Ffamilies were evaluated under two water *** quantitative trait loci(QTLs)(two for plant height,five for ASI,four for root biomass,and one for grain yield)were identified,most of which had overdominant gene *** chromosomal regions,such as those linked to markers umc1051(bin 4.08),umc2881(bin 4.03),and phi034(bin 7.02),had overlapping QTLs.

关键词： maize drought tolerance evaluation quantitative inheritance QTL

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Positional cloning of genes/loci related to plant height in soybean(Glycine Max)

Positional cloning of genes/loci related to plant height in ...

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第十三届全国植物基因组学大会

作者： Li-Fang Gao~(1,2),Yong Guo~2,Zai-Bin Hao~1,and Li-Juan Qiu~(2*) 1 Guilin University of Technology,541000 Guilin,china 2 The national key facility for crop gene resources and genetic improvement(NFCRI), Institute of crop Science,Chinese Academy of Agricultural Sciences,beijing 100081,china

Soybean(Glycine max),one of the most important economic crops in the world,is rich in protein and lipid for *** height is one of important traits related to yield in *** lots of genes/loci related to plant height have...

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A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties

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Journal of Integrative Agriculture 2016年第1期15卷 10-17页

作者： ZHAO Pei WANG Ke LIN Zhi-shan LIU Hui-yun LI Xin DU Li-pu YAN Yue-ming YE Xing-guo national key facility of crop gene resources and genetic improvement/Institute of crop Science Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China key Laboratory of genetics and Biotechnology College of Life Science Capital Normal University Beijing 100048 P R. China

Locating of important agronomic genes onto chromosome is helpful for efficient development of new wheat varieties. Wheat chromosome substitution lines between two varieties have been widely used for locating genes because of their distinctive advantages in genetic analysis, compared with the aneuploid genetic materials. Apart from the substituted chromosome, the other chromosomes between the substitution lines and their recipient parent should be identical, which eases the gene locating practice. In this study, a set of chromosome substitution lines with cv. Wichita （WI） as the recipient parent and cv. Cheyenne （CNN） as the donor parent were studied for the composition of high molecular weight glutenin subunits （HMW-GS） as well as a range of agronomic important traits. Results revealed that the substitution lines of WI（CNN5D）, WI（CNN6A） and WI（CNN7B） had higher plant heights than the two parents of WI and CNN, and WI（CNN3D） had later maturity than the parents. By sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS-PAGE） and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS） analysis, a substitution line WI（CNN5B） was found to contain different HMW-GS patterns from its two parents, in which 1 By9 was replaced by 1 By8 on chromosome 1BL. Simple sequence repeat （SSR） analysis confirmed that the variation on 1BL in WI（CNN5B） was originated from Chinese Spring （CS）. It is concluded that chromosomal fragments from bridge material and donor parent were quite often retained in intracultivaral chromosome substitution lines except the substituting chromosomes.

关键词： wheat intracultivaral chromosome substitution lines agronomic traits high molecular weight glutenin subunits(HMW-GS) molecular markers

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