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Effects of temperature and organic and inorganic nutrients on the growth of Chattonella marina (Raphidophyceae)from the Daya Bay,South china Sea

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Acta Oceanologica Sinica 2011年第3期30卷 124-131页

作者： WANG Zhaohui YUAN Meiling LIANG Yu LU Sollghui College of Life Science and Technology Jinan University Guangzhou 510632 China engineering research center of Tropical and Subtropical Aquatic Ecological engineering Ministry of Education Guangzhou 510632 China

The effects of temperature and different forms of nutrients on Chattonella marina growth have been investigated in strains isolated from the Daya Bay, the South china Sea. The strain of C. marina preferred high temperatures, with an optimal temperature of 25℃, and 18℃ was the minimum for its survival. Higher cell number and growth rate were obtained in high nitrogen and phosphorus concentrations （500 μg/L, 74μg/L） than under nutrient limitation. Nitrogen influenced the growth most, as the specific growth rate and maximum cell density were lower in nitrogen- limited cultures than noted under phosphorus limitation or under limitation from both. C. marina was capable of using many kinds of organic nitrogen sources including L-serine （L-Ser）, glycine （Gly）, alanine （Ala）, L-threonine （L-Thr）, glutamic acid （Glu） and urea, but could not utilize uric acid. Various forms of organic phosphorus compound such as glucose-6-phosphate （G6P）, sodium glycerophosphate （GYP）, adenosine triphosphate （ATP）, adenosine monophosphate （AMP）, cyti- dine monophosphate （CMP）, guanosine monophosphate （GMP）, uridine monophosphate （UMP）, 4-nitrophenylphosphate （NPP） and triethyl phosphate （TEP） supported the growth as well. Algal cells had the ability to sustain growth under nitrogenand/or phosphorus-free conditions particularly under phosphorus depleted condition. These results led to the hypothesis that high loading of nitrogen has played an important role in frequent C. marina blooms in the past decade, and its capability for utilization of diverse forms of organic nutrients and growth in low nutrient conditions make this species a likely recurrent dominant in the Daya Bay phytoplankton assemblages, visible as more frequent blooms.

关键词： Chattonella marina growth inorganic and organic nutrients salinity temperature

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Achieving efficient Channel utilization for Data Communications in IEEE 802.11 WLAN

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Transactions of Tianjin university 2005年第6期11卷 422-427页

作者：张亮舒炎泰 School of Electronic Information engineering Tianjin UniversityTianjin 300072China

In the IEEE g02. 11 protocol, the adoption of the exponential backoff technique leads to throughput performance strongly dependent on the initial contention window size and, most importantly, on the number of contending stations considered in the network. This paper proposes a simple but accurate method to dynamically estimate the number of contending stations in a wireless local area network （ WLAN ）. Based on estimation, all the mobile stations dynamically adjust the initial contention window in medium access control （ MAC ） layer to avoid collisions. The simulation results show that the proposed algorithm can achieve efficient channel utilization, higher system throughput, and better fairness performance.

关键词： IEEE 802.11 wireless local area network( WLAN ) distributed coordination function ( DCF )

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Local segment-shared protection based on source egress gateway selection for multi-domain optical mesh networks

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Science china(Information Sciences) 2011年第2期54卷 227-235页

作者： ZHANG ShengFeng1,3, PENG YunFeng1,2, DU Shu1, YANG XiaoLong1,2 & LONG KePing1,2 1research center for Optical Internet and Mobile Information Networks, university of Electronic Science and Technology of china, Chengdu 611731, china 2Institute of Advanced Network Technology and New Serives (ANTS), university of Science and Technology Beijing, Beijing 100083, china 3research center for Optical Internet Networks, Chongqing university of Posts and Telecommunications, Chongqing 400065, china 1. research center for Optical Internet and Mobile Information Networks University of Electronic Science and Technology of China Chengdu 611731 China3. Research Center for Optical Internet Networks Chongqing University of Posts and Telecommunications Chongqing 400065 China2. Institute of Advanced Network Technology and New Serives (ANTS) University of Science and Technology Beijing Beijing 100083 China

Survivability for multi-domain optical networks is becoming a key parameter due to expanding network. In order to provide the shared-path-protected lightpath in multi-domain optical mesh networks, we propose a protection solution, called GSLSSP. A new source egress gateway selecting mechanism is adopted in this solution. For each connection request, this mechanism can enable the source node to select a better egress gateway node to the destination domain and compute one inter-domain working path near the optimal path with the cooperating of this gateway node, under scalability and domain privacy constraint of multi-domain networks. And then one intra-domain link-disjoint local segment-backup path for each working-segment path is computed out in each single-domain passed by the working path. Simulation evaluation shows that the proposed scheme can provide 100% protection ability for multiple failures. Besides, it is effective in multi-domain path protection with more efficient resource utilization, lower blocking probabilities and less inter-domain cost than other schemes.

关键词： survivability optical mesh networks multi-domains multiple failures segment-shared protection

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IFNN时变模型在建立大坝位移监控中的应用

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水电能源科学 2008年第5期26卷 101-103页

作者：武金坤李波河海大学水利水电工程学院江苏南京210098 河海大学水资源高效利用与工程安全国家工程研究中心江苏南京210098

针对时变监控模型比常规监控模型更有效且能准确进行变量分离,通过改进的模糊神经网络(IFNN)结合时变模型对陈村水库大坝的水平位移进行拟合与预测,并与BP模型做了比较。结果表明,该模型拟合及预测精度均较BP模型高,与工程实例相近,可... 详细信息

针对时变监控模型比常规监控模型更有效且能准确进行变量分离,通过改进的模糊神经网络(IFNN)结合时变模型对陈村水库大坝的水平位移进行拟合与预测,并与BP模型做了比较。结果表明,该模型拟合及预测精度均较BP模型高,与工程实例相近,可推广应用。

关键词：时变模型 BP模型改进的模糊神经网络水平位移

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Genome-wide SNP in silico detection of Lentinula edodes via whole genome sequencing

Genome-wide SNP in silico detection of Lentinula edodes via ...

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中国菌物学会第五届会员代表大会暨2011年学术年会

作者： CHEN Xiang BAO Da-Peng (national research center for Edible Fungi Biotechnology and engineering Key Laboratory of Applied Mycological resources and utilization,Ministry of Agriculture,china Shanghai Key Laboratory of Agricultural Genetics and Breeding Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai,china)

＜正＞Analysis of single nucleotide polymorphisms（SNPs） emerges as an important tool in evolutionary biology and *** we report the in silico discovery and analysis of SNPs across the whole genome of two protoplast monokaryons（TPMs） of basidiomycete fungi Lentinula edodes via the high-throughput sequencing *** our knowledge,this is a novel study in which different protoplast monokaryons are utilized for SNPs detection and *** sequence information including 210M and 250M nucleotide bases was generated from TPMs yielding approximately 80%of whole genome *** used surpassed 380K and 410K reads of TPMs for the de novo assembly of two contig sets with an overall estimated depth of 5.67-fold and 6.05-fold coverage,*** of the contigs between TPMs identified 108,235 putative SNPs owning a high probability of at least 10-fold *** SNPs reveal TPMs having similar intra-strain SNP frequencies,and the ratio of transition to transversion among these SNPs being similar to the ratio reported in other *** verify the confidence of detected SNPs,25,044 SNPs were mapped onto 43,912 positions that uniformly distributed over the sequenced chromosomes of Coprinopsis *** result shows high sequence conservation among closely related fungal *** addition,the gene structure of TPMs was constructed for SNPs classification according to their distribution in genome ***,this study indicates the utility of using next generation sequencing technologies to identify large numbers of reliable ***,the method combination of SNP detection and novel algorithm allows an efficient approach for the development of high-density genotyping platforms in fungal species.

关键词： Lentinula edodes whole genome sequencing contig assembly SNPs

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高矿化度环境下黏性土对膨润土-砂土复合防渗层抗渗性的影响研究

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节水灌溉 2011年第11期 67-69,72页

作者：王晓芸崔自治张群崔永成宁夏大学旱区现代农业水资源高效利用教育部工程研究中心宁夏银川750021

以宁夏广泛分布的砂土为研究对象,以黏性土掺量c、盐浓度d、盐的复合比r、膨润土掺量p为因素,渗透系数为考察参数,设计L9(34)正交实验方案和单因素实验方案,实验研究了盐对膨润土-黏性土-砂土复合防渗层抗渗性的影响,揭示了黏性土掺量c... 详细信息

以宁夏广泛分布的砂土为研究对象,以黏性土掺量c、盐浓度d、盐的复合比r、膨润土掺量p为因素,渗透系数为考察参数,设计L9(34)正交实验方案和单因素实验方案,实验研究了盐对膨润土-黏性土-砂土复合防渗层抗渗性的影响,揭示了黏性土掺量c、盐浓度d、盐的复合比r、膨润土掺量p等因素对膨润土-黏性土-砂土复合防渗层抗渗性的作用规律和作用机理,为砂土基防渗层设计、施工提供理论依据。实验研究表明:在盐溶液环境中,膨润土的作用效应降低,黏性土的作用效应显著增强,但膨润土仍然是影响砂土基防渗层渗透性最主要的因素;土样的渗透系数随着盐溶液复合比的增大,呈现出先减小,后略微增大的趋势;黏性土使盐对砂土基防渗层的抗渗性影响显著降低,尤其是在高矿化度环境下,作用效应更为显著。

关键词：膨润土黏性土盐复合防渗

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efficient and Lossless Multicast Support in Large Routers

Efficient and Lossless Multicast Support in Large Routers

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2006 10th International Conference on Communication Technology（ICCT 2006）

作者： Yufeng Li Julong Lan Han Qiu Peng Yi national Digital Switching System engineering & Technological research center Zhengzhou Henan china 450002 Fengsen Deng School of Electronic engineering university of Electronic Science and Technology Chengdu Sichuan china 610054

Today, multicast-capable routers generally handle multicast traffic by appending each cell a local multicast output label （LMOL） containing a bitmap with as many bits as output ports, so as to identify the ports to which a copy of the cell has to be transferred. However, this approach is unfeasible for the very large IP routers having 128 output ports or more, because the LMOL length would rise above 16bytes, thus the bandwidth wasted for transmitting LMOL will be intolerable for small cells. Recently, there come forth many approaches using various lossy compression algorithms to shorten the LMOL, hence to save bandwidth and improve efficiency. In this paper, we aim to offer both lossless replication and high efficiency for multicast traffic traversing in large routers, and present a scheme characterized by dual lookups and three-stage replications to achieve the goals. Experiment results show that the scheme can support multicast replication with no loss happening for large IP routers with as many as 128×128 output ports, in condition that the internal bus width being a normal value of 128, meanwhile, the scheme can also provide the most efficient transmission and replication.

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Analysis of Bacterial Communities Present in Agaricus bisporus Phase II Compost Using Amplified Ribosomal DNA Restriction Analysis (ARDRA)(Ⅰ)——Construction of a Bacterial 16S rDNA Library

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食用菌学报 2008年第4期 16-19页

作者： HE Lihong~(1,2) YU Rongli~1 CHEN Mingjie~(1*) PAN Yingjie~3(~1Key Laboratory of Applied Mycological resources and utilization,Ministry of Agriculture,the People's Republic of china,Shanghai research center for Edible Fungi Biotechnology and engineering,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201106,china ~2Department of Microbiology,College of Life Sciences,nanjing Agricultural university,nanjing,Jiangsu 210095,china ~3Shanghai Ocean university,Shanghai 200090,china)

An efficient and cost effective method for obtaining good quality DNA from composts containing high levels of organic matter has been *** protocol consisted of sample washing with sodium phosphate and EDTA,cell wall disruption using combined chemical and enzyme treatment prior to extraction,removal of humic acid and other inhibitors in high salt buffer containing polyvinyl polypyrrolidone (PVPP),and precipitation of DNA with polyethylene glycol (PEG-8000).The method was used to extract 16S ribosomal DNA from four samples taken at different stages in the preparation of PhaseⅡcompost used for Agaricus bisporus *** DNA was sufficiently free of contaminants to allow PCR amplification of products derived from bacteria,actinomycetes and *** products derived from PCR amplification of the extracted 16S rDNA using primers F27 and R1498 were used to construct 16S rDNA *** amplification of ten randomly selected positive clones using primer M13 revealed that the 16S rDNA insertion rate was higher than 90%.

关键词： Agaricus bisporus compost phaseⅡ bacterial 16S rDNA library

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综合业务接入ATM网络的资源共享

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通信学报 2001年第9期22卷 70-77页

作者：兰巨龙邬江兴李鸥解放军信息工程大学国家数字交换系统工程技术研究中心郑州450002

本文提出了一种在ATM网络中传输综合业务的资源共享方案。该方案中 ,每一VP只传输一类业务 ,并根据各类业务的带宽、各VP传输的业务量和链路的剩余带宽动态分配各个VP占用的宽宽 ,在保证各类业务呼损率的前提下 ,可获得提高传输效率和... 详细信息

本文提出了一种在ATM网络中传输综合业务的资源共享方案。该方案中 ,每一VP只传输一类业务 ,并根据各类业务的带宽、各VP传输的业务量和链路的剩余带宽动态分配各个VP占用的宽宽 ,在保证各类业务呼损率的前提下 ,可获得提高传输效率和降低节点归一化处理负荷的良好折衷。本文还引入了迟滞和资源预留两个概念 ,并分析了它们对呼损率和节点的归一化处理负荷的影响。

关键词：综合业务接入通信网资源共享异步转移模式

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Construction of Prophylactic Human Papillomavirus Type 16 L1 Capsid Protein Vaccine Delivered by Live Attenuated Shigella flexneri Strain sh42

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Acta Biochimica et Biophysica Sinica 2005年第11期37卷 743-750页

作者： Xiao-Feng YANG Xin-Zhong QU Kai WANG Jin ZHENG L(?)-Sheng SI Xiao-Ping DONG Yi-Li WANG Key Laboratory of Biomedical Information engineering of Ministry of Education,Institute for Cancer research,Xi'an Jiaotong university,Xi'an 710061.china national Institute for Viral Disease Control and Prevention,Chinese center for Disease Control and Prevention,Beijing 100052,china Department of Obstetrics and Gynecology,First Hospital of Xi'an Jiaotong university,Xi'an 710061,china KeyLab.ofBiomedicalInformationengineeringofMinistryofEducation Inst.forCancerResearchXi'anJiaotongUnivers

To express human papillomavirus(HPV)L1 capsid protein in the recombinant strain of Shigellaand study the potential of a live attenuated Shigella-based HPV prophylactic vaccine in preventing HPVinfection,the icsA/virG fragment of Shigella-based prokaryotic expression plasmid pHS3199 was *** type 16 L1(HPV16L1)gene was inserted into plasmid pHS3199 to form the pHS3199-HPV16L1construct,and pHS3199-HPV16L1 was electroporated into a live attenuated Shigella strain *** analysis showed that HPV16L1 could be expressed stably in the recombinant strain *** test results were negative,which showed that the sh42-HPV16L1 lost ***,the at-tenuated recombinant strain partially maintained the invasive property as indicated by the HeLa cell *** IgG,IgA antibody against HPV16L1 virus-like particles(VLPs)were detected in the sera,intestinal lavage and vaginal lavage from animals immunized by *** number of antibody-secreting cells in the spleen and draining lymph nodes were increased significantly compared with the *** from immunized animals inhibited murine hemagglutination induced by HPV16L1 VLPs,whichindicated that the candidate vaccine could stimulate an efficient immune response in guinea pig's *** may be an effective strategy for the development of an HPV prophylactic oral vaccine.

关键词： human papillomavirus type 16 attenuated Shigella flexneri vaccine cervical cancer prophylactic

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