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Comparative research of anthraquinone,triphenylmethane and azo dyes decolorization by a newly isolated Bacillus cereus strain DC11

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Comparative research of anthraquinone,triphenylmethane and a...

microbial carbonates in Holocene beachrocks,Shuiweiling,Luhuitou Peninsula,Hainan Island

第十次全国环境微生物学术研讨会

作者： Daiyong Deng Jun Guo Guoqu zeng Guoping Sun Daiyong Deng South china Botanical Garden,chinese academy of sciences,Guangzhou 510650,china Guangdong institute of microbiology,Guangzhou 510070,china china Graduate School of the chinese academy of sciences,beijing 100039,china Guangdong Provincial Key laboratory of microbial Culture Collection and Application,Guangzhou 510070,china Jun Guo,Guoqu zeng,Guoping Sun Guangdong institute of microbiology,Guangzhou 510070,china

A broad-spectrum dye-decolorizing and degradation bacterium,strain DC11,was isolated and identified as a member of Bacillus cereus from activated *** important parameters including pH,temperature,oxygen concentration and carbon sources were comparatively studied using anthraquinone,triphenylmethane,and azo dyes,*** color removal,the suitable pH and temperature were 6.0-8.0 and 20-45℃,*** than 95% of 100 μM Acid Blue 25 (anthraquinone dye) was anaerobic degraded within 8 *** than 95% of 750 μM Basic Blue X-GRRL (azo dye) was anaerobic decolorized within 2 *** strain could also decolorize 98% of the 55 μM Malachite Green (triphenylmethane dye) within 6 h under microaerophilic or anaerobic *** fraction decolorizing assays showed that the color removal of these dyes was contributed to the soluble cytosolic fraction,and these functional components showed NADH/NADPH-dependent *** products were analyzed with *** intermediate metabolite was presented before the anthraquinone bone breakage in Acid Blue 25 degradation;Malachite Green was degraded into 4,4’-bis(dimethylamino)benzophenone and benzophenone;and Basic Blue X-GRRL decolorization was mainly due to the azo bond breakage.

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Science china Earth sciences 2008年第1期51卷 30-40页

作者： TENG JianBin1,2 & SHEN JianWei1 1 Department of Marine Geology, South china Sea institute of Oceanology CAS Key laboratory of Marginal Sea Geology, South china Sea institute of Oceanology, chinese academy of sciences, Guangzhou 510301, china 2 Graduate University of the chinese academy of sciences, beijing 100049, china Department of Marine Geology South China Sea Institute of Oceanology CAS Key laboratory of Marginal Sea Geology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301 China Graduate University of the chinese academy of Sci Department of Marine Geology South China Sea Institute of Oceanology CAS Key laboratory of Marginal Sea Geology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301 China

Two research methods, geomicrobiology and carbonate sedimentology, were adopted to study the microbial carbonates in the beachrocks, Shuiweiling, Luhuitou, Sanya City, Hainan Island. The results indicate that microbial carbonates occur in Shuiweiling beachrocks and cements related to microbial activitives are common. microbial carbonates were mainly developed on the surfaces of coral skeletons and within the coral (fragments and shivers) skeletal cavities,and rarely grew in interspaces formed by coral and other biological detritus. The growth morphologies of microbial carbonates are various, including stromatolitic lamination, thin crusts, dome-like, and microbialites. microbial carbonates commonly trap fine-sized bioclasts, contain tubular filaments, and show laminated growth striation. microbial carbonates within the coral skeletal cavities are generally stromatolitic cone-shaped, with a random growth direction along the bearing of trend of cavities, showing distinct growth laminae and recognizable growth phases. It is a special growth pattern of crypt microbial carbonates. microbial cements occur mainly in shape of needle cement, calcified filaments, microborings, and calcified framboidal spheres. The needle cement is the most common type. These microbial cements indicate that microbial activities and microbial carbonates played an important role in beachrock formation in which carbonates were dominant components and the climate of tropical ocean monsoon was influential. Such microbial carbonates and microbial cementation have not been reported here from beachrocks along the northern shorelines of the South china Sea. The results support a clear microbial origin for certain carbonate cements in beachrocks, confirm that microbial carbonates are a significant contributor to carbonate sedimentation, and widen the knowledge of beachrocks from a new field.

关键词： microbial carbonates microbial cements beachrock Holocene Hainan Island

Distribution of Soil Organic Carbon Fractions Along the Altitudinal Gradient in Changbai Mountain,china

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Pedosphere 2011年第5期21卷 615-620页

作者： ZHANG Min ZHANG Xiao-Ke LIANG Wen-Ju JIANG Yong DAI Guan-Hua WANG Xu-Gao HAN Shi-Jie State Key laboratory of Forest and Soil Ecology Institute of Applied Ecology Chinese Academy of Sciences Shenyang 110164 (China) Vegetable Research institute Liaoning Academy of Agricultural Sciences Shenyang 110161 (China) Graduate University of chinese academy of sciences Beijing 100049 (China)

Understanding the responses of soil organic carbon(SOC) fractions to altitudinal gradient variation is important for understanding changes in the carbon balance of forest *** our study the SOC and its fractions of readily oxidizable carbon(ROC),water-soluble carbon(WSC) and microbial biomass carbon(MBC) in the soil organic and mineral horizons were investigated for four typical forest types,including mixed coniferous broad-leaved forest(MCB),dark coniferous spruce-fir forest(DCSF),dark coniferous spruce forest(DCS),and Ermans birch forest(EB),along an altitudinal gradient in the Changbai Mountain Nature Reserve in Northeast *** results showed that there was no obvious altitudinal pattern in the *** variation trends of SOC with altitude were observed between the organic and mineral *** differences in the contents of SOC,WSC,MBC and ROC were found among the four forest types and between *** contents of ROC in the mineral horizon,WSC in the organic horizon and MBC in both horizons in the MCB and EB forests were significantly greater than those in either DCSF or DCS *** proportion of soil WSC to SOC was the lowest among the three main *** contents of WSC,MBC and ROC were significantly correlated(P < 0.05) with SOC *** can be concluded that vegetation types and climate were crucial factors in regulating the distribution of soil organic carbon fractions in Changbai Mountain.

关键词： active carbon fractions altitude forest ecosystem Northeast china vegetation type

Cloning and sequencing of nifBHDKENX genes of Paenibacillus massiliensis T7 and its nif promoter analysis

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Science china(Life sciences) 2006年第2期49卷 115-122页

作者： ZHAO Hongxin1,2,3, XIE Baoen2 & CHEN Sanfeng1,2 1. State Key laboratory for Agrobiotechnology and Biological School, china Agricultural University, beijing 100094, china 2. Key laboratory for Agro-microbial Resource and Application of Agriculture Ministry, china Agricultural University, beijing 100094, china 3. Chemical and Biological School, ShenYang Normal University, Shenyang 110034, china State Key laboratory for Agrobiotechnology and Biological School China Agricultural University Beijing China Key laboratory for Agro-microbial Resource and Application of Agriculture Ministry China Agricultural University Beijing China Chemical and Biological School ShenYang Normal University Shenyang China

A 324 bp of nifH fragment was PCR amplified from Paenibacillus massiliensis T7 using the universal degenerate primers. The PCR-amplified nifH fragment was labeled with DIG; then used as a probe in Southern blot analysis. Southern blot result showed that there were two positive signals, indicating that there might be two copies of nifH in P. massiliensis T7. A total of 10254 bp DNA sequence containing purD; nifBHDKENX was obtained by five rounds of inverse-PCR amplification. The predicted proteins of nifBHDKENX had high homology with those from other nitrogen-fixing bacteria. Only one putative σ[54]-dependent promoter sequence was detected upstream of the nifB gene; nifBHDKENX were likely to be organized in one operon. Assays of β-galactosidase activity of P. massiliensis T7PB carrying a nifB-lacZ fusion under different concentrations of NH4 [+]; O2 showed that the expression of nifB-lacZ was strongly inhibited by O2.

关键词： Paenibacillus massiliensis T7, nifBHDKENX, nifB-lacZ, inverse-PCR.

NAVIGATED BY METAGENOMICS:A CASE STUDY OF THE microbial COMMUNITY IN THE SOUTH china SEA

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NAVIGATED BY METAGENOMICS:A CASE STUDY OF THE MICROBIAL COMM...

Recent Research Advances in Cordyceps Biology

2008年中国微生物学会学术年会

作者： Chengzhang Fu~(1,4),Jiang Bian~(1,2,4),Nuo Sun~(1,3,4),Gang Pei~(1,4),HongLing Chen~1,Jian Wang~(1,2,4),Biao Ren~(1,4),FuHang Song~1,LiXin Zhang~(1,2,4) 1.institute of microbiology,chinese academy of sciences,beijing,100101,P.R.china. 2.South china Sea institute of Oceanology,chinese academy of sciences,Guangzhou,510301,P. R.china. 3.institute of Oceanology,chinese academy of sciences,Qingdao 266071,china. 4.Graduate University of chinese academy of sciences,beijing,100049,P.R.china.

Background and aims:Recently,oceans have been found as unexploited treasure troves comparing to their terrestrial *** aim to analyze the microbial community in samples obtained from the South china Sea to guide the construction of metagenomic libraries and subsequent study of unknown environmental functional genes.

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食用菌学报 2006年第2期 93-98页

作者： LIN Qun-ying~ 1, 2, 3 , ZHONG Yue-jin~ 2 , LI Tai-hui~ 2* , SHEN Ya-heng~ 2 , SONG Bin~ 2 ~ 1 South china Botanic Garden, Academica Sinica, Guangzhou 510650, china ~ 2 Guangdong institute of microbiology, Guangdong Provincial Key laboratory of microbial Culture Collection and Application, Guangzhou 510070, china ~ 3 Graduate University of the chinese academy of sciences, beijing 100049, china

Recent research advances in Cordyceps biology, including strain isolation and correlation with corresponding teleomorphs, fermentation studies, stroma cultivation and strain breeding, have been reviewed. Developing tr... 详细信息

关键词： Anamorph Cordyceps Liquid culture Strain breeding Stroma cultivation

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土壤微生物多样性及其环境影响因子研究进展

生态学杂志 2005年第1期24卷 48-52页

作者：张薇魏海雷高洪文胡跃高中国农业科学院畜牧研究所中国农业大学农学与生物技术学院北京100094 中国科学院微生物研究所中国农业大学农学与生物技术学院

土壤中存在丰富的微生物资源 ,不同土壤系统具有不同的微生物群落 ,微生物多样性既依赖于生态系统又服务于生态系统。本文从物种多样性、遗传多样性、生态类型多样性、功能多样性四个方面对土壤微生物多样性进行了新的诠释 ,总结论述了... 详细信息

土壤中存在丰富的微生物资源 ,不同土壤系统具有不同的微生物群落 ,微生物多样性既依赖于生态系统又服务于生态系统。本文从物种多样性、遗传多样性、生态类型多样性、功能多样性四个方面对土壤微生物多样性进行了新的诠释 ,总结论述了土壤微生物多样性与环境因子如土壤、植物群落和气候条件之间的关系 ,并对目前存在的问题和今后面临的挑战提出几点看法。

关键词：土壤微生物多样性环境因子

Influence of Panax ginseng Continuous Cropping on Metabolic Function of Soil microbial Communities

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chinese Herbal Medicines 2012年第4期4卷 329-334页

作者： YING Yi-xin1,DING Wan-long1,ZHOU Ying-qun2,LI Yong1 1.institute of Medicinal Plant Development,chinese academy of Medical sciences and Peking Union Medical College,beijing 100193,china 2.china National Corporation of Traditional and Herbal Medicine,beijing 100195,china institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193 China china National Corporation of Traditional and Herbal Medicine Beijing 100195 China

Objective To investigate the influence of Panax ginseng continuous cropping on the carbon substrate metabolic activity of microbes in soils sampled from Dafang,Huangni,and Wulidi in Jilin Province,*** Soil metabolisms of soil communities were characterized by community level physiological profiles using BIOLOGTM *** Soils sampled from the three sites were analyzed and their metabolic activities were *** component analysis explored the significant variance in metabolic function of microbial communities in soils,though the Shannon index and the evenness index of them were ***,two principal components(PC1 and PC2),which contributed 67.83% and 10.78% of total variance,were extracted *** also,substrates significantly correlated with PC1 and PC2 at the three sampling sites were *** Characteristic of soil is the primary factor influencing microbial communities,and *** continuous cropping has significant influence on microbial *** soil samples show similar microbial metabolic profiles,microbial communities in rhizosphere soil are changed obviously during the cultivation of ***,which would finally result in the unbalance of microbial *** would gradually be the predominants in rhizosphere soil and make *** sick.

关键词： continuous cropping metabolic function microbial community Panax ginseng rhizosphere soil

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nisZ启动子结构与功能的研究

微生物学报 2003年第2期43卷 194-199页

作者：杨巍陈秀珠袁静还连栋中国科学院微生物研究所分子微生物学研究中心北京100080 中国科学院微生物研究所分子微生物学研究中心和微生物资源前期开发国家重点实验室北京100080

应用β Glucuronidase基因 (gusA)作为报告基因 ,通过定点突变方法分别缺失nisZ编码区上游两个启动子结构 (promoter1和promoter2 )中的一个 ,发现只有靠近编码区的promoter2是nisZ启动子诱导表达所必需。将promoter2中 1 0区及其上游... 详细信息

应用β Glucuronidase基因 (gusA)作为报告基因 ,通过定点突变方法分别缺失nisZ编码区上游两个启动子结构 (promoter1和promoter2 )中的一个 ,发现只有靠近编码区的promoter2是nisZ启动子诱导表达所必需。将promoter2中 1 0区及其上游的一个碱基突变为乳酸菌中典型的组成型启动子的 1 0区结构 ,该改变使nisZ启动子诱导功能下降 ;将promoter2的 1 0区和 35区的间隔区由 2 0个碱基缺失突变为 1 7个碱基 ,则nisZ启动子失去诱导功能。

关键词： nisZ启动子功能 gusA 结构