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The cessation and detoxification effect of tea filters on cigarette smoke

Science china(Life Sciences) 2010年第5期53卷 533-541页

作者： YAN JingQi1,2,DI XiaoJing1,4,LIU CaiYi3,ZHANG HuiMin3,HUANG XiouQin3,ZHANG JunJing1,2,ZHAO Yan5,ZHANG LongZe1,CHANG YanZhong4,LIANG YongLin2$,TAO Ran3 & ZHAO BaoLu1,21 State Key Laboratory of Brain and Cognitive Science,institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,china 2 Key Laboratory of Mental Health,institute of Psychology,Chinese Academy of Sciences,Beijing 100101,china 3 Addiction Branch,Beijing Military Region General Hospital,Beijing 100700,china 4 institute of molecular neurobiology,hebei normal university,shijiazhuang 050016,china 5 Department of Food Sciences and Technology,Harbin institute of Technology at Weihai,Weihai 264209,china 1. State Key Laboratory of Brain and Cognitive Science Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China2. Key Laboratory of Mental Health Institute of Psychology Chinese Academy of Sciences Beijing 100101 China4. Institute of Molecular Neurobiology Hebei Normal University Shijiazhuang 050016 China3. Addiction Branch Beijing Military Region General Hospital Beijing 100700 China5. Department of Food Sciences and Technology Harbin Institute of Technology at Weihai Weihai 264209 China

To treat tobacco addiction,a tea filter was developed and studied for smoking *** work reports the smoking cessation effect of tea when it was used as a component of cigarette *** one trial it was found that after using the tea filters for 2 months,the volunteer smokers decreased their cigarette consumption by 56.5%,and 31.7% of them stopped *** work identified a new method and material,tea filter and theanine,which inhibit tobacco and nicotine addiction and provide an effective strategy for treating tobacco addiction.

关键词： cigarette smoking addiction cigarette cessation tea filter theanine nicotine dependence nicotine acetylcholine receptor(nAChR) dopamine public health free radical

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钩藤水提物Batch-2对6-羟多巴胺诱导的SH-SY5Y细胞损伤的神经保护作用(英文)

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生物化学与生物物理进展 2010年第7期37卷 769-778页

作者：石振华 XI Zhao-Wilsor 王浩关鹏于鹏常彦忠段相林赵保路河北师范大学生命科学学院石家庄050016 BioMarker Pharmaceuticals Inc.5941 Optical CourtSan JoseCA 95138USA 北京普尼公司北京100080 中国科学院生物物理研究所北京100101

以前的药理学研究表明,钩藤水溶性提取物C-MED-100TM不仅具有抗氧化活性,而且还具有很好的DNA修复和免疫功能.Batch-2是一种新的水溶性钩藤提取物,而它的自由基清除能力及神经保护作用还未见报道.首先检测了batch-2对六羟多巴胺诱导的SH... 详细信息

以前的药理学研究表明,钩藤水溶性提取物C-MED-100TM不仅具有抗氧化活性,而且还具有很好的DNA修复和免疫功能.Batch-2是一种新的水溶性钩藤提取物,而它的自由基清除能力及神经保护作用还未见报道.首先检测了batch-2对六羟多巴胺诱导的SH-SY5Y细胞损伤的神经保护作用机制,然后利用红外光谱、HPLC和分光光度技术对batch-2的组成成分进行了分析.结果表明,batch-2具有清除各种自由基的能力,尤其是对羟自由基的清除(25mg/L的batch-2对羟自由基的清除率为60%),batch-2可剂量依赖性地抑制6-羟多巴胺诱导的细胞凋亡、脂质过氧化水平、线粒体膜电位的降低和细胞内活性氧和一氧化氮的增加.同时,batch-2抑制了由6-羟多巴胺诱导的SH-SY5Y细胞内iNOS和NF-κB蛋白的上调.结果表明,batch-2对六羟多巴胺诱导的SH-SY5Y细胞损伤的神经保护作用是通过清除活性氧和一氧化氮、抑制iNOS和NF-κB表达实现的.成分分析表明,batch-2中的多酚和奎宁酸含量分别为6.43%和0.0958%.上述结果显示,batch-2的抗氧化机制部分类似于EGCG.对于帕金森病的预防,batch-2是一个潜在具有很好的神经保护作用的天然抗氧化剂.

关键词：绒毛钩藤(Uncaria tomentosa) 抗氧化剂帕金森氏病活性氧 6-羟多巴胺电子顺磁共振

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The universality and biological significance of signal molecules with intracellular-extracellular compatible functions

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Chinese Science Bulletin 2000年第19期45卷 1729-1734页

作者： SUN Dayeinstitute of molecular Cell Biology, hebei normal university, shijiazhuang 050016, china institute of molecular Cell Biology Hebei Normal University Shijiazhuang China

Generally, cell signal molecules are classified into the extracellular signal molecules (the first messengers) and the intracellular signal ones (the second messengers). Cyclic adenosine monophosphate (cAMP), calcium ions and calmodulin (CaM) are the traditional intracellular messengers, but they are also present in extracellular matrix (ECM). Some of them have been discovered to act as the first messengers through cell surface receptors. Other second messengers, such as cyclic guanosine monophosphate (cGMP), cyclic adenosine diphosphate ribose (cADPR) and annexin, are also found existing outside animal and plant cells. The existence of these messengers with intracellular-extracellular compatible functions in cells may be a regular biological phenomenon. These compatible messengers might be the communication factors between intracellular and extracellular regions or among the cell populations, and are also important in regulating cell development procedure.

关键词： intracellular signal extracellular signal compatible messenger.

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Synthesis and Stereostructure of New β-Lactam Derivatives of1,5-Benzothiazepines

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Chinese Chemical Letters 1999年第6期10卷 447-448页

作者： Yuan LI Na SUN Sheng JIN(Department of Chemistry hebei university shijiazhuang 050016 institute of Chemistry and molecular Engineering Peking university Beijing 100871) hebei normal Univ Dept Chem Shijiazhuang 0550016 Peoples R China Peking Univ Inst Chem & Mol Engn Beijing 100871 Peoples R China

Reaction of 1,5-benzothiazepine with N-protected glycine gives new a-amino-β-lactamderivatives of 1.5-benzothiazepine. The configuration and conformation of the products wereconfirmed by x-ray diffraction. The resu... 详细信息

关键词： 1.5-Benzothiazepine β-lactam stereospecific reaction

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Progress in the participation of Ca2+-calmodulin in heat shock signal transduction

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Progress in Natural Science:Materials International 2009年第10期19卷 1201-1208页

作者： Rengang Zhou a,b,Bing Li c,d,Hongtao Liu a,c,Daye Sun c,d,a institute of Genetics and Physiology,hebei Academy of Agricultural Sciences,shijiazhuang 050051,china b hebei Provincial Key Laboratory of Plant Genetic Engineering,shijiazhuang 050051,china c Academy of Life Sciences,hebei normal university,shijiazhuang 050016,china d hebei Provincial Key Laboratory of molecular and Cell Biology,shijiazhuang 050016,china [a]institute of Genetics and Physiology Hebei Academy of Agricultural Sciences Shijiazhuang 050051 China [b]hebei Provincial Key Laboratory of Plant Genetic Engineering Shijiazhuang 050051 China [c]Academy of Life Sciences Hebei Normal University Shijiazhuang 050016 China [d]hebei Provincial Key Laboratory of molecular and Cell Biology Shijiazhuang 050016 China

A novel heat shock(HS) signal transduction pathway in plants for the participation of Ca2+-calmodulin(CaM) in HS signal trans-duction was *** induces a rapid increase in intracellular free calcium ion levels([Ca2+]i),and the involvement of phospholipase Cinositol 1,4,5-trisphosphate is one of the factors leading to elevation in [Ca2+]i induced by *** also increases the expression of the CaM gene and the accumulation of the CaM *** CaM isoform,AtCaM3,in Arabidopsis is a key member in the HS signal trans-duction ***3 regulates the activity of CaM-binding protein kinase(AtCBK3) or protein phosphatase(AtPP7),promoting the activation of the HS transcription factor,AtHSFA1a,by phosphorylation/dephosphorylation and the expression of heat shock pro-tein genes,then improving heat tolerance in plants.

关键词： Ca2+ Calmodulin Protein kinase Heat shock transcription factor Signal transduction

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植物类整合素蛋白研究进展

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生物化学与生物物理进展 2001年第3期28卷 283-286页

作者：孙颖孙大业河北师范大学生命科学学院分子细胞生物学研究室石家庄050016

整合素是动物细胞膜上普遍存在的一类胞外基质受体 ,它所介导的粘附作用参与调节多种细胞功能 .近年来的研究发现在植物细胞中可能也存在类整合素 .综述了在植物类整合素检测、定位、组成、结构、基因以及生理功能研究方面所取得的初步... 详细信息

整合素是动物细胞膜上普遍存在的一类胞外基质受体 ,它所介导的粘附作用参与调节多种细胞功能 .近年来的研究发现在植物细胞中可能也存在类整合素 .综述了在植物类整合素检测、定位、组成、结构、基因以及生理功能研究方面所取得的初步进展与存在的问题 ,并与动物整合素的研究作了比较 .

关键词：类整合素植物质膜生理功能蛋白亚基

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拟南芥钙调素定点突变基因分离及其在钙不依赖钙调素结合蛋白检测中的应用(英文)

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生物化学与生物物理进展 2009年第7期36卷 890-896页

作者：高丽王振杰崔素娟河北师范大学分子细胞生物学研究室河北省分子细胞生物学重点实验室

动植物系统研究表明,钙调素不仅在结合钙离子时调节多种靶酶或靶蛋白的活性,而且没有钙离子结合时,还可以通过结合钙不依赖的钙调素结合蛋白,发挥多种生物学作用.然而,目前却没有体内分析钙调素与钙不依赖钙调素结合蛋白相互作用的方法... 详细信息

动植物系统研究表明,钙调素不仅在结合钙离子时调节多种靶酶或靶蛋白的活性,而且没有钙离子结合时,还可以通过结合钙不依赖的钙调素结合蛋白,发挥多种生物学作用.然而,目前却没有体内分析钙调素与钙不依赖钙调素结合蛋白相互作用的方法.首先,采用定点突变的方式,得到了拟南芥钙调素亚型2的多个突变基因mCaM2,随后,大肠杆菌重组表达突变蛋白的电泳迁移率及45Ca2+覆盖分析表明,得到了编码失去钙结合能力的钙调素的突变基因mCaM21234,mCaM21234突变钙调素中所有4个钙结合EF-hand结构域中的关键氨基酸谷氨酸均突变为谷氨酰胺.在酵母双杂交体系中,作为诱饵蛋白的突变钙调素mCaM21234与我们前期体外方法报道的钙不依赖性钙调素结合蛋白AtIQD26存在相互作用.这将为钙不依赖性钙调素结合蛋白提供有用的体内研究工具,有利于我们全面认识钙-钙调素-钙调素结合蛋白信号途径.

关键词：定点突变拟南芥钙调素钙不依赖钙调素结合蛋白

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Primary evidence for involvement of IP3 in heat-shock signal transduction in Arabidopsis

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Cell Research 2006年第4期16卷 394-400页

作者： Hong Tao Liu~(1,2,*) Fei Gao~(1,2,*) Shu Juan Cui~2 Jin Long Han~(1,2) Da Ye Sun~2 Ren Gang Zhou~1 ~1 institute of Genetics and Physiology,hebei Academy of Agricultural Sciences,shijiazhuang 050051,china ~2institute of molecular Cell Biology,hebei normal university,shijiazhuang 050016,china instituteofGeneticsandPhysiology HebeiAcademyofAgriculturalSciencesShijiazhuang050051China instituteofmolecularCellBiology HebeiNormalUniversityShijiazhuang050016China

The role of inositol 1,4,5-trisphosphate （IP3） in transducing heat-shock （HS） signals was examined in *** whole-plant IP3level increased within 1 min of HS at 37℃.After 3 min of HS,the IP3level reached a maximum2.5 fold *** the transgenic Arabidopsis plants that have AtHsp18.2plvmoter-β-glucuronidase （GUS） fusiongene,it was found that the level of GUS activity was up-regulated by the addition of caged IP3at both non-HS and HStemperatures and was down-regulated by the phospholipase C （PLC） inhibitors {1-[6-((17β-3-Methoxyestra-1,3,5（10）-trien-17-yl)amino)hexyl]-2,5-pyrrolidinedione} （U-73122）.The intracellular-free calcium ion concentration ([Ca2+]i) increased during HS at 37℃ in suspension-cultured Arabi-dopsis cells expressing *** with U-73122 prevented the increase of [Ca2+]ito some *** provided primary evidence for the possible involvement of IP3in HS signal transduction in higher plants.

关键词： heat shock signal transduction IP3 PLC [Ca2+]i Arabidopsis

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Effects of Intranigral Injection of Glutamate/GABA on Iron Metabolism in the Nigrostriatum of Rats

Effects of Intranigral Injection of Glutamate/GABA on Iron M...

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第九次全国暨2007海内外生物膜学术研讨会

作者： Peng Guan~(1 2) Xiang-Lin Duan~(1 2) Na Wang~(1 2) Yan-Zhong Chang~(1 2) 1 The institute of molecular neurobiology and Neuropharmacology hebei normal university shijiazhuang 050016 hebei Province china 2 The key labortry of Animal Physiology Biochemistry and molecular Biology of hebei Province hebei normal university shijiazhuang 050016 hebei Province china

＜正＞To investigate the effects of intranigral increased glutamate/GABA on iron metabolism in the nigrostriatum of rats,Glutamate Mono-Sodium（GMS）and GABA was respectively stereotaxically injected into substantia n... 详细信息

关键词： Glutamate GABA DMTI(-IRE) Hephaestin

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Identification and Characterization of a Rat Novel Gene RSEP4 Expressed Specifically in Central Nervous System

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Acta Biochimica et Biophysica Sinica 2004年第7期36卷 501-507页

作者： Xi-Dao WANG1,2, Ling-Wei KONG1, Zhi-Qin XIE1, Yu-Qiu ZHANG3, Zhi-Xin LIN2, Zhi-Qi ZHAO3,Lei YU4, and Nai-He JING1* 1 Laboratory of molecular Cell Biology, Laboratory of Stem Cell Biology, institute of Biochemistry and Cell Biology, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, china School of Life Science and Biotechnology, 2 Shanghai Jiaotong university, Shanghai 200030, china institute of neurobiology, Fudan university, Shanghai 200433, china 3 4 Department of Cell Biology, neurobiology and Anatomy, College of Medicine, university of Cincinnati, Cincinnati, Ohio, USA Laboratory of molecular Cell Biology and Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai China

The low-abundantly expressed genes composed the majorities of the mRNAs expressed in the central nervous system (CNS), and were thought to be important for the normal brain functions. Through differential screening a low-abundance cDNA sublibrary with mRNA from neuropathic pain of chronic constriction injury (CCI) model, we have identified a novel rat gene, rat spinal-cord expression protein 4 gene (RSEP4). The total length of RSEP4 cDNA is 2006 bp, with a 501 nucleotide open reading frame (ORF) that encodes a 167 amino acid polypeptide. Northern blot revealed that RSEP4 was expressed specifically in the CNS. In situ hybridization showed that the mRNA of RSEP4 was strongly expressed in the CA1, CA2, CA3 and DG regions of hippocampus, the Purkinje cells of cerebellum, and the small sensory neurons of dorsal horn and large motor neurons of ventral horn of spinal cord. Over-expression of RSEP4-EGFP fusion protein in the human embryonic kidney 293T cells showed that RSEP4 protein was mainly localized in the cell cytoplasm. These results suggest that RSEP4 may play some roles in the CNS.

关键词： central nervous system (CNS) rat Northern blot in situ hybridization

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