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Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis

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science china(life sciences) 2008年第7期51卷 604-613页

作者： XU YueYu 1,2,3,ZHOU YuLei 4,5 ,SONG LinLin 6 ,ZHANG Yan 3 &ZHAO MaoLin 1 1 Beijing Agro-Biotechnology Research Center,Beijing Academy of agriculture and Forestry science,Beijing 100097,china 2 Department of Chemistry and Chemical Engineering,Hunan Institute of Engineering,Xiangtan 411104,china 3 college of life science,Capital Normal university,Beijing 100037,china 4 college of life science,zhongkai university of agriculture and technology,guangzhou 510225,china 5 college of Grassland science,Gansu Agricultural university,Lanzhou 730070,china 6 Department of Biology,Henan Institute of science and technology,Xinxiang 453003,china 1. Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Science Beijing 100097 China 2. Department of Chemistry and Chemical Engineering Hunan Institute of Engineering Xiangtan 411104 China 3. college of life science Capital Normal University Beijing 100037 China 4. college of life science Zhongkai University of Agriculture and Technology Guangzhou 510225 China 5. college of Grassland science Gansu Agricultural University Lanzhou 730070 China 6. Department of Biology Henan Institute of Science and Technology Xinxiang 453003 China

Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in *** order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC747H/sacB,which contain about 165000 and 236000 recombinant clones *** genome coverage of the two libraries was totally estimated to be about 3―5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 300 *** of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3×3 pattern using a GeneTAC?G3 arraying robot after being transferred manually into three 384-well *** 2501 and 2890 clones of Library in pYLTAC17 and in pYLTAC747H/sacB were stored individually in fourteen 384-well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of *** positive clones were detected by using the probe glutahione reductase gene of *** libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional *** the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system.

关键词： Leymus multicaulis megabase-size DNA transformation-competent artificial chromosome(TAC) genomic library

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Pulsed current-assisted twelve-roll precision rolling deformation of SUS304 ultra-thin strips with exceptional mechanical properties

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International Journal of Extreme Manufacturing 2024年第4期6卷 291-305页

作者： Wanwan Fan Tao Wang Jinxiong Hou zhongkai Ren Qingxue Huang Guanghui Wu college of Mechanical and Vehicle Engineering Taiyuan University of TechnologyYingze West Street 79Taiyuan 030024Shanxi ProvincePeople's Republic of China Engineering Research Center of Advanced Metal Composites Forming technology and Equipment of Ministry of Education Taiyuan University of TechnologyYingze West Street 79Taiyuan 030024Shanxi ProvincePeople's Republic of China National Key Laboratory of Metal Forming technology and Heavy Equipment Taiyuan University of TechnologyYingze West Street 79Taiyuan 030024Shanxi ProvincePeople's Republic of China college of Aeronautics and Astronautics Taiyuan University of TechnologyYingze West Street 79Taiyuan 030024Shanxi ProvincePeople's Republic of China

Innovative pulsed current-assisted multi-pass rolling tests were conducted on a 12-roll mill during the rolling deformation processing of SUS304 ultra-thin *** results show that in the first rolling pass,the rolling reduction rate of a conventionally rolled sample(at room temperature)is 33.8%,which can be increased to 41.5%by pulsed current-assisted rolling,enabling the formation of an ultra-thin strip with a size of 67.3μm in only one rolling *** three passes of pulsed current-assisted rolling,the thickness of the ultra-thin strip can be further reduced to 51.7μ*** clearly compare the effects of a pulsed current on the microstructure and mechanical response of the ultra-thin strip,ultra-thin strips with nearly the same thickness reduction were *** was found that pulsed current can reduce the degree of work-hardening of the rolled samples by promoting dislocation detachment,reducing the density of stacking faults,inhibiting martensitic phase transformation,and shortening the total length of grain *** a result,the ductility of ultra-thin strips can be effectively restored to approximately 16.3%while maintaining a high tensile strength of 1118 ***,pulsed current-assisted rolling deformation shows great potential for the formation of ultra-thin strips with a combination of high strength and ductility.

关键词： pulsedcurrent-assisted SUS304 ultra-thinstrip rolling reductionrate work-hardening mechanical properties

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A folic acid-decorated nanoparticles loaded JQ1 for oral squamous cell carcinoma therapy

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Chinese Chemical Letters 2024年第3期35卷 332-336页

作者： Dequan Zeng zhongkai Ma Xin Zan Ting Luo Xiang Wang Xiang Gao Xianghui Fu School of Health Management Xihua UniversityChengdu 610039China State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China College of StomatologySichuan UniversityChengdu 610041China Department of Neurosurgery and Institute of Neurosurgery State Key Laboratory of Biotherapy and Cancer CenterWest China HospitalWest China Medical SchoolSichuan University and Collaborative Innovation Center for BiotherapyChengdu 610041China Breast Disease Center Cancer CenterWest China HospitalSichuan UniversityChengdu 610041China

Oral squamous cell carcinoma(OSCC)is known as one of the most malignant tumors with high recurrence and fatality *** poor tumor-targeting ability of traditional chemotherapeutic drugs has been a grand challenge for anti-OSCC *** that,a large quantity of tumor associated macrophages in OSCC tissues further diminish the anti-tumor effects of these ***,we produced a therapeutic nano drug delivery system(FA-PEG-PLA-JQ1)through encapsulating JQ1[a small-molecule inhibitor of bromodomain containing protein 4(BRD4)]into the folic acid(FA)-modified nanoparticle(PEG-PLA),which could prolong the half-life of JQ1 and target the tumor *** then,JQ1 released from this nanoparticle could prevent OSCC growth inducing tumor cell apoptosis,inhibiting tumor angiogenesis and the polarization of M2 type *** conclusion,our date demonstrated the therapeutic benefits of FA-PEG-PLA-JQ1 against OSCC in vivo or in vitro,which could be a novel treatment strategy for OSCC in coming days.

关键词： Folic acid Nanoparticles JQ1 Oral squamous cell carcinoma Macrophages

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Adsorption Breakthrough of Benzene in the Fixed Bed of Modified Activated Carbon under Different Humidity Conditions

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Journal of Wuhan university of technology(Materials science) 2010年第3期25卷 499-503页

作者：李晶 college of Chemistry and Chemical Engineering Zhongkai University of Agriculture and Engineering

The breakthrough curves of benzene and water on modified activated carbons（ACs） were ***-programmed desorption（TPD） experiments were conducted to measure the TPD curves of benzene and water on modified and unmodified ACs and to estimate the activation energy for the desorption of benzene on the modified *** with unmodified ACs,two modified ACs were prepared by using two different types of silane,designated by KH560 and *** results showed that the activation energy for the desorption of benzene on KH560/AC and 1706/AC was higher than that on unmodified *** addition,the activation energy for the desorption of water on KH560/AC and 1706/AC was lower than that on unmodified *** breakthrough curves of benzene obtained from the experimental observations under different humidity conditions were compared with the results of the TPD *** results show that the modified ACs are less affected by water,whereas the unmodified ACs are more affected by water,indicating that surface modification by organosilane compounds can improve the adsorption of benzene on the activated carbo,which weakens the adsorption of water.

关键词： activated carbon benzene water TPD activation energy of desorption breakthrough curves

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A temporal framework for building up of healthy soils

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Frontiers of Agricultural science and Engineering 2024年第2期11卷 292-296页

作者： Junling ZHANG Jiangzhou ZHANG Yunlong ZHANG guangzhou WANG State Key Laboratory of Nutrient Use and Management College of Resources and Environmental SciencesNational Academy of Agriculture Green DevelopmentChina Agricultural UniversityBeijing 100193China Inner Mongolia Key Laboratory of Soil Quality and Nutrient Resources College of GrasslandResources and EnvironmentInner Mongolia Agricultural UniversityHohhot 010018China Key Laboratory of Grassland Management and Utilization Ministry of Agriculture and Rural Affairs of the People’s Republic of ChinaCollege of Grassland Science and TechnologyChina Agricultural UniversityBeijing 100193China

Healthy soils are vital for the diverse services provided by ecosystems to human society~[1], such as provisioning(food,fiber, timber, and fuel), regulation(climate, disease, and natural hazards), waste treatment, nutrient cycling, and cultural services. Soil has a three phase system and the interactions between soil chemical, biological, and physical attributes determine functions(hydraulic, thermal, and gas fluxes).

关键词： soils soil cycling

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Enabling superior electrochemical performance of NCA cathode in Li_(5.5)PS_(4.5)Cl_(1.5)-based solid-state batteries with a dual-electrolyte layer

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Chinese Chemical Letters 2024年第4期35卷 231-236页

作者： Ziling Jiang Shaoqing Chen Chaochao Wei Ziqi Zhang zhongkai Wu Qiyue Luo Liang Ming Long Zhang Chuang Yu School of Chemistry and Chemical Engineering Huazhong University of Science and TechnologyWuhan 430074China State Key Laboratory of Advanced Electromagnetic Engineering and technology School of Electrical and Electronic EngineeringHuazhong University of Science and TechnologyWuhan 430074China Innovation Center for Chemical sciences College of ChemistryChemical Engineering and Materials ScienceSoochow UniversitySuzhou 215123China college of Physics and Energy Fujian Normal UniversityFuzhou 350117 China

LiNi_(0.8)Co_(0.15)Al_(0.05)O_(2)(NCA) is a promising cathode for sulfide-based solid-state lithium batteries(ASSLBs)profiting from its high specific capacity and voltage plateau, which yielding high energy density. However, the inferior interfacial stability between the bare NCA and sulfides limits its electrochemical performance. Hereien, the dual-electrolyte layer is proposed to mitigate this effect and enhance the battery performances of NCA-based ASSLIBs. The Li_(3)InCl_6 wih high conductivity and excellent electrochemcial stability act both as an ion additives to promote Li-ion diffusion across the interface in the cathode and as a buffer layer between the cathode layer and the solid electrolyte layer to avoid side reactions and improve the interface stability. The corresponding battery exhibits high discharge capacities and superior cyclabilities at both room and elevated temperatures. It exhibits discharge performance of 237.04 and216.07 m Ah/g at 0.1 and 0.5 C, respectively, when cycled at 60 ℃, and sustains 95.9% of the capacity after100 cycles at 0.5 C. The work demonstrates a simple strategy to ensure the superior performances of NCA in sulfide-based ASSLBs.

关键词： LiNi_(0.8)Co_(0.15)Al_(0.05)O_(2) Li_(5.5)PS_(4.5)Cl_(1.5) Double solid electrolyte layer configuration Operating temperatures Electrochemical performances

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Empirical Analysis of Factors Influencing Compensation for Non-commercial Forests under CVM

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Asian Agricultural Research 2012年第7期4卷 1-6,12页

作者： ZHANG Mei college of Economics and Trade,zhongkai university of agriculture and Engineering,guangzhou 510225,china

Based on non-commercial forests in Kunming City,this article conducts survey of 506 households in Kunming City to evaluate their willingness to pay ecological benefit of non-commercial forests,using Contingent Valuation Method(CVM);to analyze factors influencing their willingness to compensate for non-commercial forests,using multivariate statistical analysis *** results show that income,educational level, housing ownership,outdoor exercise time,whether to be concerned about environmental problems,frequency of obtaining forest-related information monthly and whether understanding non-commercial forests or not,are the most principal factors influencing willingness to pay.

关键词： Non-commercial forests Ecological benefit compensa

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Soils for food security

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Frontiers of Agricultural science and Engineering 2024年第2期11卷 213-215页

作者： guangzhou WANG Qichao ZHU Junling ZHANG State Key Laboratory of Nutrient Use and Management National Academy of Agriculture Green DevelopmentCollege of Resources and Environmental SciencesChina Agricultural UniversityBeijing 100193China

Food security is among the most fundamental of national interests and productive farmland is essential for secure grain *** the increase of agricultural intensification and the impact of global climate change,the soil... 详细信息

关键词： farmland security earth

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Large-scale information entropy analysis of important sites in mature and precursor miRNA sequences

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science china(life sciences) 2009年第8期52卷 771-779页

作者： DU JiangFeng1,2,WU YongJun2,ZHANG YinXia3,WU Lei1,2,WANG XiaoLong3,TAO ShiHeng1,2 1 Bioinformatics Center,Northwest science and technology university of agriculture,Yangling 712100,china 2 college of life sciences,Northwest science and technology university,Yangling 712100,china 3 college of science,Northwest science and technology university,Yangling 712100,china Bioinformatics Center Northwest Science and Technology University of Agriculture Yangling 712100 China college of life sciences Northwest Science and Technology University Yangling 712100 China college of science Northwest Science and Technology University Yangling 712100 China

In order to find evidence of consistent sequence conservation or the base correlation degree in miRNA,some important sites in the sequences of reported miRNA and their precursors(pre-miRNA) were investigated via information entropy *** different groups of sites were obtained from special locations(head,tail) in miRNAs of different sources according to taxonomy(animal,plant and virus) and then analyzed by measuring the single base information redundancy(D1(L)) and the adjacent base related information redundancy(D2(L)).The results showed that D2(L) has more information than D1(L),though D1(L) changes roughly consistently with D2(L) in each *** pre-miRNAs are more conservative than those belonging to animals or *** addition,U is dominant in most sites compared with other *** was also found that in the middle of several groups,there were sites where miRNAs were cut down from pre-miRNAs by Enzyme Dicer which were significantly *** phenomenon shows that the conservatism is an aspect of the of miRNA and may be involved in the recognition and cutting by the *** results provided another perspective for understanding more about the primary structure of pre-miRNA.

关键词： miRNA conservative information entropy precursor evolution base correlation degree

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b-DAZL:A novel gene in bovine spermatogenesis

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Progress in Natural science:Materials International 2008年第10期18卷 1209-1218页

作者： Qingbo Zhang~a Qifa Li~(a,*) Jiahuang Li~b Xinfu Li~a Zhenshan Liu~a Dawei Song~a Zhuang Xie~a ~a Laboratory of Animal Reproductive Genetics and Molecular Evolution,college of Animal science and technology,Nanjing agriculture university,Nanjing 210095,china ~b Pharmaceutic Biotechnology Key Laboratory,college of life science,Nanjing university,Nanjing 210093,china Laboratory of Animal Reproductive Genetics and Molecular Evolution College of Animal Science and Technology Nanjing Agriculture University Nanjing 210095 China Pharmaceutic Biotechnology Key Laboratory College of Life Science Nanjing University Nanjing 210093 China

The DAZL gene plays an important role in *** and mutation of this gene has been frequently associated with the sterility in both vertebrates and ***,a cDNA containing the complete open reading frame of DAZL gene,which encodes putative RRM RNA-binding protein,was isolated from bovine tissues by *** isolated gene was referred to as ***-based location analysis showed that b-DAZL is located near the end of chromosome *** b-DAZL gene and its vertebrate homologue shared a high nucleotide similarity (90-94%) in the coding region and in the genomic organization,each consisting of 10 exons and 9 *** protein products contained a highly conserved RNA-binding motif,a unique DAZ repeat, and PUM2 and PABP interacting ***-PCR assay revealed that b-DAZL was expressed in the testis and ***,no signals were detected in the testes of sterile male *** microstructure of the testes of sterile males showed very few spermat- ocytes but mostly somatic *** observations are consistent with the typical phenotype of testes with defective DAZL expression. Subsequent gene detection studies indicated that transcription arrest might lead to a b-DAZL transcript defect in testes of cattle-yaks. Our results suggest that b-DAZL may be involved in spermatogenesis,and that transcription arrest of the gene is associated with male sterility in cattle-yaks.

关键词： Cattle-yak b-DAZL Male sterility Sequence analysis Expression profile

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