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Hepatic blood inflow occlusion without hemihepatic artery control in treatment of hepatocellular carcinoma

World Journal of Gastroenterology 2010年第46期16卷 5895-5900页

作者： Shan Jin,Department of General Surgery,affiliated hospital of Inner Mongolia medical college,Hohhot 010050,Inner Mongolia Autonomous Region,china Chao-Liu Dai,Department of Hepatobiliary Surgery,Shengjing hospital,china medical University,Shenyang,Liaoning Province,china Department of General Surgery Affiliated Hospital of Inner Mongolia Medical College Hohhot 010050 Inner Mongolia Autonomous Region China. jinshangood@***

AIM:To investigate the clinical significance of hepatic blood inflow occlusion without hemihepatic artery control (BIOwHAC) in the treatment of hepatocellular carcinoma (HCC).METHODS:Fifty-nine patients with HCC were divided into 3 groups based on the technique used for achieving hepatic vascular occlusion:group 1,vascular occlusion was achieved by the Pringle maneuver (n=20);group 2,by hemihepatic vascular occlusion (HVO) (n=20);and group 3,by BIOwHAC (n=19).We compared the procedures among the three groups in term of operation time,intraoperative bleeding,postoperative liver function,postoperative complications,and length of hospital ***:There were no statistically significant differences (P > 0.05) in age,sex,pathological diagnosis,preoperative Child's disease grade,hepatic function,and tumor size among the three *** intraoperative complications or deaths occurrred,and there were no significant intergroup differences (P > 0.05) in intraoperative bleeding,hepatic function change 3 and 7 d after operation,the incidence of complications,and length of hospital *** and Pringle maneuver required a significantly shorter operation time than HVO;the difference in the serum alanine aminotransferase or aspartate aminotransferase levels before and 1 d after operation was more significant in the BIOwHAC and HVO groups than in the Pringle maneuver group (P < 0.05).CONCLUSION:BIOwHAC is convenient and safe;this technique causes slight hepatic ischemia-reperfusion injury similar to HVO.

关键词： Hepatic blood inflow occlusion without hemihepatic artery control Hepatocellular carcinoma Intraoperative bleeding Ischemia-reperfusion injury

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Molecular Analysis of the Huntington's Disease with Expanded CAG Trinucleotide Repeat in Chinese

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High Technology Letters 1995年第1期1卷 5-9页

作者： Zeng Yitao Mao Yuehua Chen Meijue Ren Zhaorui Zhou Gang Huang Shuzhen (Shanghai Institute of medical Genetics,Shanghai Children’s hospital,Shanghai 200040,P.R.china) Wang Xiuying~① Yie Wenghu~② Zhao Xiangzhi~③ (①xuzhou medical college,xuzhou,JiangSu,P.R.china) (②Anhui medical University,Aanhui,P.R.china) (③Henan Psychiatrical Institute,Zhengzhou,P.R.china) Shanghai Inst of medical Genetics Shanghai China

The polymorphic CAG repeats in the IT15 gene in Chinese normal and Huntington’s dis-ease(HD)chromosomes were determined by using nested PCR and denaturing polyacry-lamide gel electrophoretic autoradiography as well as direct sequencing analysis.A total of40 normal individuals and 122 members of 13 unrelated HD families originating from Shang-hai,Jiangsu,Zhejiang,Anhui,Shandong,Guangdong and Henan,respectively,were in-volved in this *** results showed that the(CAG)n repeat numbers in 270 normal al-leles ranged from 13 to 26 but most in 16;while in 54 HD alleles,the CAG repeats from 40to 94,with an unstable inheritance of expanded repeats in some *** was no over-lap between the normal and affected ***,the presymptomatic diagnosis in103 family members at risk for HD disclosed that 35 individuals had HD alleles,which were-in accordance with the pedigree analysis and clinical *** these results indicatedthat the dynamic mutation in IT15 gene was responsible for the genetic defect in the ChineseHD patients and that a correlation existed between the numbers of(CAG)n repeat and theonset age of the ***-of these provide valuable data for HD molecular diagnosis,ge-netic counselling and genetic health.

关键词： Huntington’s disease (HD) IT15 gene trinucleotide repeat.

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Hyaluronic acid fragments evoke Kupffer cells via TLR4 signaling pathway

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Science china(Life Sciences) 2009年第2期52卷 147-154页

Kupffer cells, expressing toll-like receptor 4 (TLR4), play a central role in hepatic ischemia/reperfusion (I/R) injury. Hyaluronic acid (HA) fragments, degradative products of high-molecular-weight HA (HMW-HA), acquire the ability to activate immune cells under inflammatory conditions. Here we inves- tigated whether HA fragments could activate Kupffer cells and analyzed the underlying mechanism. Kupffer cells were isolated from wild-type mice (WT, C3H/HeN) and TLR4 mutant mice (C3H/HeJ) and HA fragments were produced by the methods of enzyme digestion and chromatography. Then Kupffer cells were stimulated by HA fragments or other control stimuli. The activation of Kupffer cells was estimated as the release of pro-inflammatory cytokines. The activation of p38 MAPK pathway of Kupffer cells was checked and blocking experiments were done as well. The results indicated that HA fragments acquired the ability to activate Kupffer cells in vitro, which was TLR4 dependent and not due to contamination of lipopolysaccharide. Experiments of p38 MAPK kinase inhibition by SB-203580 verified p38 MAPK was required in HA fragments induced Kupffer cells activation. This suggests that HA fragments, degradative products of one of the major glycosaminoglycans of the extracellular matrix, play critical roles in Kupffer cell activation mediated by TLR4 signaling pathway, which is, at least partially, de- pendent on p38 MAPK activation.

关键词： Kupffer cells hyaluronic acid fragments toll-like receptor 4 p38 MAPK lipoplysaccharide reperfusion injury liver

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A mutation in signal peptide of rat resistin gene inhibits differentiation of 3T3-L1 preadipocytes

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Acta Pharmacologica Sinica 2004年第12期25卷 139-145页

作者： Xi-rong GUO2,3, Hai-xia GONG2, Yan-qin GAO4, Li FEI, Yu-hui NI, Rong-hua CHEN Department of Pediatrics, center of Human Functional Genomics, Nanjing medical University, Nanjing 210029, china 4Department of Pediatrics, the Third affiliated hospital of Nanjing medical University, Yizheng 211900, china Department of Pediatrics Center of Human Functional Genomics Nanjing Medical University Department of Pediatrics the Third Affiliated Hospital of Nanjing Medical University

AIM: To detect the resistin expression of white adipose tissue in diet-induced obese (DIO) versus diet-resistant (DR)rats, andto investigatethe relationshipof mutatedresistin and 3T3-L1 preadipocytes differentiation. METHODS: RT-PCR and Western Blot were used to detect gene /protein expression. 3T3-L1 cells were cultured, transfected, and induced to differentiation using 0.5 mmol/L 3-isobutyl-1-methyxanthine (MIX), 1 mg/L insulin, and 1 μmol/L dexamethasone. Oil red O staining was applied to detect the degree of preadipocytes differentiation. RESULTS: Expression of resistin mRNA was upregulated in DIO rats and downregulated in DR rats. However, the expression levels varied greatly within the groups. Sequencing of the resistin genes from DIO and DR rats revealed a Leu9Val (C25G) missense mutation within the signal peptide in one DR rat. The mutant resistin inhibited preadipocyte differentiation. Local experiments and Western blotting with tagged resistin fusion proteins identified both mutant and wild type proteins in the cytoplasm and secreted into the culture medium. Computer predictions using the Proscan and Subloc programs revealed four putative phosphorylation sites and a possible leucine zipper motif within the rat resistin protein. CONCLUSION: Resistin-increased differentiation may be inhibited by the mutation- containing precursor protein, or by the mutant non-secretory resistin isoform.

关键词： resistin mutation adipocyte differentiation obesity

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Protective effect of endogenous hydrogen sulfide against oxidative stress on gastric ischemia-reperfusion injury in rats

Protective effect of endogenous hydrogen sulfide against oxi...

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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Ji-He ZOU~(1,2),Wei-Li QIAO~1,Chang-Dong YAN~(1,*) 1 Department of Physiology,xuzhou medical college,xuzhou 221002,china 2 Department of Neurobiology,xuzhou medical college,xuzhou 221002,china

Objective:To observe the protective effect of endogenous hydrogen sulfide on gastric mucosal injury followed by gastric ischemia-reperfusion(GI-R) in ***:Adult male Sprague-Dawley(SD) rats were ***-R injury was induced by clamping the celiac artery of rats for 30 min and followed by reperfusing for 60 *** mucosal damage was analyzed with macroscopic injured area and deep damage was assessed with histopathology *** expressions of hydrogen sulfide synthase(CBS,CSE),SOD,XOD and subunit of NADPH oxidase(gp91,p67) in rat gastric mucosa were determined by Western ***:Pretreatment with DL-propargylglycine(PAG) andβ-cyano-L-alanine(BCA,two inhibitors of HS biosynthesis) for 30 min, gastric mucosal injury area and deep damage caused by gastric ischemia-reperfusion significantly increased compared with vehicle group(P43.5%and 25%compared with vehicle group (P<0.05).In PAG or BCA treated group,the expression of gastric mucosal SOD decreased by 30%,whereas XOD, gp91 and p67 significantly increased compared with the vehicle group(P<0.05).Conclusion:Endogenous hydrogen sulfide could protect the gastric ischemia- reperfusion injury in rats by increasing the expression of SOD and decreasing the expression of XOD and subunit of NADPH oxidase(gp91,p67).

关键词： gastric ischemia-reperfusion endogenous hydrogen sulfide SOD XOD NADPH oxidase

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Effects of H2S on cerebral arterioles myogenic responses in aging

Effects of H2S on cerebral arterioles myogenic responses in ...

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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Lei LIU~1,You-Jian QI~1,Dong SUN~2,Chang-Dong YAN~(1,△) 1 Departments of Physiology,xuzhou medical Vollege,xuzhou,Jiangsu,221002,Chin 2 Jiangsu Province Key laboratory of Anesthesiology,xuzhou medical college,xuzhou,Jiangsu,221002,china

Objective:The potential biological significance of hydrogen sulfide(HS) has attracted growing interest in recent *** aim of this study was to illustrate the effect of HS on myogenic response and the role of HS in ***:Responses to changes in intravascular pressure of isolated rat cerebral arterioles were investigated under no-flow *** arterioles of young and old rats were isolated and *** diameters were measured with an image-shearing device and then *** response to step increases in perfusion pressure(from 20 to 120 mmHg,by 20 mmHg steps),arterioles constricted and developed active tone. Results:Isolated cerebral arterioles from young and old rats exhibited myogenic responses through the pressure ranges of 20 to 120 mmHg,and the old rats arterioles decreased their myogenic tones than the arterioles from young *** myogenic tones were reduced by NaHS in both young and old *** young group,glibenclamide the K channel blockers,significantly reduced the HS-induced attenuation of myogenic tones,the blockade of nitric oxide synthase further increased HS-induced attenuation of myogenic tones,and the cAMP synthesis inhibitor SQ22536 did not change the effect of HS,***,in old group,all these inhibitors did not change the effect of HS on myogenic *** of the endothelium significantly reduced NaHS-induced reduction of myogenic response in both young and old ***:Our results demonstrate that the effect of HS on myogenic response is partially mediated through K channel in young rats but not in old rats,and HS can attenuated myogenic tones through the way of endothelial cells.

关键词： cerebral arterioles myogenic reactivity hydrogen sulfide aging

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Isoflurane preconditioning protects against ischemia-reperfusion injury partly by attenuating cytochrome c release from subsarcolemmal mitochondria in isolated rat hearts

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Acta Pharmacologica Sinica 2005年第7期26卷 813-820页

作者： Li-ping QIAN Shan-shan ZHU~2 Jun-li CAO Yin-ming ZENG Jiangsu Provincial Key Lab of Anesthesiology,Jiangsu Institute of Anesthesiology,xuzhou medical college,xuzhou 221002,china Jiangsu Provincial Key Lab of Anesthesiology Jiangsu Institute of Anesthesiology Xuzhou Medical College Xuzhou 221002 China

Aim:To examine if isoflurane preconditioning can attenuate ischemia/reperfusioninjury by reducing cytochrome c release from inner mitochondrial ***:Isolated hearts of Sprague-Dawley rats were perfused on *** were randomly assigned to a non-treated group(CON group,n=12)or three isoflurane preconditioning groups(0.5% ISC group,1.0% ISC group,and 2.0% ISC group;n=12).In the latter three groups,isoflurane was given atconcentrations of 0.5%,1.0%,and 2.0% for 15 min with 15-rain washout before30-min *** mitochondria of the myocardium were isolatedafter 60-min *** of the each heart was recorded,infarctsize of the hearts and contents of cytosolic cytochrome or mitochondrial cyto-chrome c were measured at the end of *** of isolated mito-chondria in the four groups was evaluated,***:Compared withthe CON group,cytosolic cytochrome c in 0.5% ISC group,1.0% ISC group,and2.0% ISC group were significantly decreased along with a significant increase ofmitochondrial cytochrome *** size of the hearts in the four groups were56%±12%,41%±12%,32%±7% and 33 %±11%,*** values of thethree isoflurane preconditioning groups were significantly lower than that of theCON group(P<0.05).Isoflurane exposure before ischemia can attenuate the changeof morphology of mitochondria after *** effects of 2.0% isofluraneon reducing cytochrome c release were more remarkable than 0.5% and 1.0%concentrations of ***:Myocardioprotective effects ofisofluranc preconditioning were associated with attenuation of cytochrome c lossfrom the inner membrane of subsarcolemmal mitochondria.

关键词： isoflurane reperfusion injury cytochromec mitochondria

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A novel method to label the cerebrospinal fluid-contacting neurons in rats

A novel method to label the cerebrospinal fluid-contacting n...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Jia-You WANG~#,Fang ZHOU~#,Li-Cai ZHANG~* Jiangsu Province Key laboratory of Anesthesiology,xuzhou medical college,xuzhou 221002,china

＜正＞Objective The study aimed to explore a specific marker to label cerebrospinal fluid-contacting neurons （CSF-CNs）in rat and provide a new way to identify *** Adult Sprague-Dawley rats,male,were randomly received 4 different treatment（n=6）:*** toxin subunit B（CB）injection into the cerebrospinal fluidcontacting nucleus（CSF-CN）;*** injection into the hippocampus（non-CSF-CN）as control;*** injection into the lateral ventricle;*** labeling method（lateral ventricle injection of CB-conjugated horseradish peroxidase （CB-HRP））as a positive *** received same volume of CB or CB-HRP（1μL）.Animals were perfused after 48 h survival and the relevant brain tissue was sectioned（40μm）for immunofluorescence to observe the effects of different labeling *** Group A:Neurons labeled by CB clustered only in the CSF-CN,not in other areas;Group B:In the hippocampus（non-CSF-CN area）,there were few CB positive neurons;Group C:CB positive neurons clustered in the CSF-CN;D Group:Neurons labeled by CB-HRP appeared only in the *** is,the method of CB injected into lateral ventricle has the same effects on labeling area with CB-HRP;the method of CB injected into CSF-CN has the same effects with C/D group;the method of CB injected into non-CSF-CN showed that neither CSF-CNs nor non-CSF-CNs was *** Parenchyma injection of CB is a scientific and reliable method to label CSF-CNs in rats.

关键词： CSF-CNs specific label CB

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Community-based prevention and treatment of cardiovascular diseases

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Journal of Geriatric Cardiology 2024年第3期21卷 315-322页

作者： Sheng-Shou HU The Writing Committee of the Report on Cardiovascular Health and Diseases in china Fuwai hospital National Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

The Annual Report on Cardiovascular Health and Diseases in china(2022)intricate landscape of cardiovascular health in *** is the third section of the report with a specific focus on community-based prevention and treatment of cardiovascular diseases(CVD).This section of the report underscores the importance of initiatives outlined in the"Healthy china 2030 Plan,"emphasizing the comprehensive prevention and control strategy for chronic diseases.A key aspect of this plan involves the establishment of national demonstration areas aimed at comprehensive prevention and control of chronic *** 2020,488 such areas had been set up across china,surpassing the initial target and covering a significant proportion of counties and *** report highlights the successful implementation of these strategies in Lishan district,Anshan city,where demonstration areas for comprehensive prevention and control of chronic diseases were launched in *** the course of seven years,the number of healthy units increased substantially,leading to improvements in managing risk factors for CVD among *** reductions in prevalence rates of overweight,obesity,smoking,passive smoking,and drinking were observed,along with the development of healthier behaviors among ***,Qiaokou district in Wuhan City,designated as a national demonstration area in 2014,implemented comprehensive public health promotion ***,special clinics for hypertension intervention were established,contributing to an increase in self-reported rates of hypertension,a slight decrease in prevalence,and a remarkable improvement in the control rate among treated ***,these efforts underscore the effectiveness of community-based approaches in driving positive health outcomes and advancing the comprehensive prevention and control of chronic diseases,particularly cardiovascular diseases,in china.

关键词： prevention district treatment

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Cardiovascular Risk Factors in china

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Journal of Geriatric Cardiology 2024年第2期21卷 153-199页

作者： Sheng-Shou HU the Writing Committee of the Report on Cardiovascular Health and Diseases in china Fuwai hospital National Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina 不详

The Annual Report on Cardiovascular Health and Diseases in china(2022) intricate landscape of cardiovascular health in china. This section dissects cardiovascular risk factors in china which including hypertension, dyslipidemia, diabetes mellitus, chronic kidney disease, metabolic syndrome and air pollution. Hypertension prevalence has steadily increased in china,with efforts to control it facing challenges in achieving optimal rates, especially in rural areas. Interventions like salt substitutes and intensive blood pressure control show promise but need improvement. Abnormal lipid levels, indicative of dyslipidemia,have risen significantly, posing a risk for cardiovascular diseases. Despite efforts, many patients struggle to achieve target lipid levels, necessitating improved treatment strategies. Both type 1 and type 2 diabetes mellitus affect millions of adults in china,with long-term complications adding to the disease burden. Early intervention and effective management are crucial to mitigate its impact. Prevalent among older adults, chronic kidney disease is associated with diabetes mellitus, hypertension, and cardiovascular diseases, necessitating comprehensive management approaches. The prevalence of metabolic syndrome, characterized by a cluster of risk factors, has increased in both adults and adolescents, calling for lifestyle modifications and public health interventions. Ambient and household air pollution remain significant environmental risk factors, despite some improvements in air quality. Continued efforts to reduce emissions are essential for mitigating associated health risks. Addressing these risk factors requires a multifaceted approach, including public health initiatives, policy interventions, and individual-level strategies to promote healthy lifestyles and reduce environmental exposures. Surveillance and research efforts are crucial for monitoring trends and developing effective strategies to lessen the burden of cardiovascular diseases in

关键词： landscape adding spite

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