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Possible Effect of 30K Proteins in Embryonic Development of Silkworm Bombyx mori

Acta Biochimica et Biophysica Sinica 2005年第5期37卷 355-361页

作者： Bo-Xiong ZHONG~1 Jian-Ke LI~(1.2) Jian-Rong LIN~3 Jian-She LIANG~4 Song-Kun SU~1 Hai-Sheng XU~1 Hai-Yan YAN~1 Ping-Bo ZHANG~5 Hiroshi FUJII~5 1 Laboratory of Molecular Biology of Silkworm and Honeybee, Department of Bioresource Science, co.lege of Animal Science,zhejiang university, Hangzhau 310029. china 2 Institute of Apicultural Research, Chinese Academy of Agricultural Science, Beijing 100093, china 3 Department of Sericulture, South china Agricultural university.Guangzhou 510642, china 4 biochemistry co. ltd. of zhejiang university, hangzhou 310029. china 5 Faculty of Agriculture,Kyushu university, Fukuoka 812-81. Japan Laboratory of Molecular Biology of Silkworm and Honeybee Department of Bioresource Science College of Animal Science Zhejiang UniversityHangzhou 310029 China Institute of Agicultural Research Chinese Academy of Agricultural ScienceBeijing 100093 China Department of Sericulture South China Agricultural UniversityGuangzhou 510642 China biochemistry co. ltd. of zhejiang universityhangzhou 310029 China Faculty of Agriculture Kyushu UniversityFukuoka 812-81 Japan

The silkworm Bombyx mori possesses a 30K protein family of 3×10～4 Da,the biologicalfunctions of which have not been fully *** relationship between the 30K protein family and theembryonic development of temperature sensitive sex-linked mutant strain of silkworm was investigated bytwo dimensional polyacrylamide gel electrophoresis(2D-PAGE)and Matrix assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF MS).The results show that protein spots 1-5 of the 30Kprotein family,mainly existing in normal strain,are possibly related to embryonic *** earlyco.sumption of a 30K protein named 6G1-30K-1 and the accumulation of 30K proteins named 6G1-30K-3and 6G1-30K-4 are likely caused by the destruction of physiological balance in normal embryonic development,which may lead to lower hatchability of the temperature sensitive *** results suggest that reasonablemetabolism of 30K proteins is a prerequisite for the embryo's normal development.

关键词： silkworm Bombyx mori 30K protein embryonic development 2D-PAGE MALDI-TOF MS

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DEFORMING METRICS WITH POSITIVE CURVATURE BY A FULLY NONLINEAR FLOW

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Acta Mathematica Scientia 2011年第1期31卷 159-171页

作者：岳赟盛为民 china Mobile Group zhejiang co. Ltd. Department of Mathematics Zhejiang University

By studying a fully nonlinear flow deforming co.formal metrics on a co.pact and co.nected manifold, we prove the long time existence and the exponential co.vergence of the solutions of the flow for any initial metric ... 详细信息

关键词： Fully nonlinear flow co.formal metrics Schouten tensor

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Expression and Identification of Mutated Osteoprotegerin in Culture Cells and Larvae of Silkworm

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Acta Biochimica et Biophysica Sinica 2004年第5期36卷 331-335页

作者： Hai-Long XIAO, Yong-Feng JIN, and Yao-Zhou ZHANG1* Institute of biochemistry, co.lege of Life Sciences, zhejiang university, hangzhou 310029. china co.lege of Life Sciences, zhejiang university of Science & Technology, hangzhou 310018, china Institute of biochemistry College of Life Sciences Zhejiang University Hangzhou 310029 China co.lege of Life Sciences Zhejiang University of Science & Technology Hangzhou 310018 China

The mutated osteoprotegerin (OPG-372) gene was inserted into the baculovirus transfer vector pBacPAK8, and the reco.binant plasmid was co.transfected with linearized Bm-BacPAK6 virus DNA into BmN cells, then homologous reco.bination occurred inside the cells. The reco.binant virus BmNPV-OPG- 372 was screened and identified by Southern blotting. The reco.binant human OPG-372 was expressed in cultured cells and the larvae of silkworm by inoculation of reco.binant virus. The expression products were run on the SDS-PAGE and their immunoreactivities were determined by Western blotting. It was found that a 42 kD reco.binant protein was expressed in BmN cells and a 46 kD one in larvae respectively. The bioactivi- ties of the reco.binant proteins were determined by hypocalcemic effect assay in the mice. The results showed that the reco.binant proteins had a significant hypocalcemic effect on mice sera.

关键词： OPG-372 silkworm baculovirus expression system hypocalcemic effect

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Inhibitory effects of cryptoporus polysaccharide on airway co.striction, eosinophil release,and chemotaxis in guinea pigs

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Acta Pharmaco.ogica Sinica 2004年第4期25卷 105-109页

作者： Xiao-yan ZHAO, Qiang-min XIE2, Ji-qiang CHEN, Chuan-kui KE3 zhejiang Respiratory Drugs Research Laboratory of State Drugs Administration of china, Medical School ofzhejiang university, hangzhou 310031 3TSBIO Science& Technology co.ltd. hangzhou 310006, china 浙江大学国家食品药品监督管理局呼吸药物研究实验室 Hangzhou 310031 TSBIO Science & Technology co Ltd Hangzhou 310006

AIM: To study effects of cryptoporus polysaccharide (CP) on antigen-induced bronchoco.striction, eosinophil peroxidase (EPO) release in vivo, and on platelet activating factor (PAF)-induced eosinophil chemotaxis in vitro in guinea pig. METHODS: The asthma model of guinea pig was formed with ovalbumin (OVA). The changes of lung resistance (RL) and dynamic lung co.pliance (Cdyn), EPO level in bronchoalveolar lavage fluids (BALF) and eosino- phil migration were determined. RESULTS: Pretreatment of CP at doses of 3, 9, and 27 mg/kg by intragastric gavage (ig), qd for 10 d, inhibited early asthma response in a dose-dependent manner. Inhibitory rates of mean increase value from 1 to 30 min of RL were 34.8 %, 74.4 % (P40.5 % (Pco.centrations of 0.13, 1.3, 13, 130 nmol/L to eosinophil migration induced by PAF were 6.8 %, 17.2 % (Pco.CLUSION: CP protects lung against increase of RL and reduction of Cdyn, decreases EPO level in the asthma model, and inhibits eosinophil chemotaxis induced by PAF. The results suggest that CP may be a novel antiinflammatory agent for the treatment of asthma and allergic diseases.

关键词： polysaccharide ovalbumin bronchoco.striction respiratory function tests eosinophil peroxidase platelet activating factor chemotaxis asthma guinea pig

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天然软黏土屈服面及流动法则试验研究

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土木工程学报 2009年第4期42卷 119-127页

作者：沈恺伦王立忠浙江浙峰工程咨询有限公司浙江杭州310012 浙江大学浙江杭州310027

天然沉积的软黏土往往具有结构性和塑性各向异性，通过对K固结温州软黏土的19组三轴排水应力路径试验和4组三轴不排水剪切试验的研究，揭示典型结构性软黏土的屈服特性和屈服后的塑性流动特征。试验研究表明在各种三轴应力路径下，天然... 详细信息

天然沉积的软黏土往往具有结构性和塑性各向异性，通过对K固结温州软黏土的19组三轴排水应力路径试验和4组三轴不排水剪切试验的研究，揭示典型结构性软黏土的屈服特性和屈服后的塑性流动特征。试验研究表明在各种三轴应力路径下，天然软黏土的应力应变曲线均表现出明显的结构屈服特征和塑性各向异性。典型的天然结构性软黏土的初始屈服面在p’～q平面上呈现出倾斜椭圆的形状，可以采用Wheeler等建议的屈服面方程描述，而屈服后塑性流动方向基本符合正交流动法则。同时对温州软黏土在较大等向应力作用下屈服面的旋转作了试验研究，试验结果揭示了该过程中原有的塑性各向异性逐渐消失、土体呈现出塑性各向同性性状的规律，表明天然软黏土具有明显的旋转硬化规律，而后继塑性变形符合正交流动法则。

关键词：软黏土结构性塑性各向异性屈服面塑性流动旋转硬化

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Thermal Shock Resistances of FeMnCr/Cr_3C_2 co.tings Deposited by Arc Spraying

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Journal of Wuhan university of Technology(Materials Science) 2010年第2期25卷 243-247页

作者：罗来马郦剑 Derpartment of Materials Science and Engineering Zhejiang University

Arc spraying with the co.ed wires was applied to deposit FeMnCr/Cr3C2 co.tings on low carbon steel substrates, namely FM1, FM2 and FM3. Thermal shock resistances of the co.tings were investigated to assess the influence of Cr3C2 co.tent on thermal shock resistance. Characteristics of the co.tings under thermal cycling test were studied by optical microsco.y, field emission scanning electron microsco.e （FE-SEM） and energy dispersion spectrum （EDS）, X-ray diffraction （XRD）. The experimental results show that hardness of the co.tings increases, bonding strength decreases slightly with increase of the Cr3C2 co.tent of the co.tings. As a result, FM2 co.ting possesses the best thermal shock resistance, attributing to its better thermal expansion matches and wettability than those of FM3 co.ting, less oxide rate than that of FM1 co.ting restraining from cracks formation and propagation in co.tings.

关键词： arc swaying Cr3C2 particles FeMnCr/Cr3C2 co.ting thermal shock resistance

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Functional Characterization of a Putative Nitrate Transporter Gene Promoter from Rice

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Acta Biochimica et Biophysica Sinica 2006年第11期38卷 795-802页

作者： Ting-Zhang HU~(1,3) Kai-Ming CAO~1 Mian XIA~2 Xi-Ping WANG~(1,2*) 1 Department of biochemistry,School of Life Science Fudan university,Shanghai 200433,china 2 National Center for Molecular Crop Design,Weiming Kaituo Agriculture Biotech co.,ltd.,Beijing 100085,china 3 Department of Biology,Chongqing Three Gorges university,Chongqing 404000,china Department of biochemistry School of Life Science Fudan UniversityShanghai 200433 China Department of Biology Chongqing Three Gorges UniversityChongqing 404000 China National Center for Molecular Crop Design Weiming Kaituo Agriculture Biotech Co. Ltd.Beijing 100085 China

Drought is one of the most significant abiotic stresses that influence plant growth *** analysis revealed that OsNRT1.3,a putative nitrate transporter gene in rice,wasinduced by *** co.firm if the OsNRT1.3 promoter can respond to drought stress,a 2019 bpupstream sequence of OsNRT1.3 was *** OsNRT1.3 promoter fragments were generated by5′-deletion,and fused to the β-glucuronidase (GUS) *** chimeric genes were introduced into ***2019::GUS,NRT1196::GUS and NRT719::GUS showed similar expression patterns in seeds,roots,leaves and flowers in all transgenic rice,and GUS activity co.ferred by different OsNRT1.3 promoterfragments was significantly upregulated by drought stress,indicating that OsNRT1.3 promoter responds todrought stress and the 719 bp upstream sequence of OsNRT1.3 co.tains the drought response elements.

关键词： deletion analysis drought nitrate transporter OsNRT1.3 promoter

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Thoughts on the Underground Space Planning and co.struction,the co.e Area of Qianjiang New CBD,hangzhou

Thoughts on the Underground Space Planning and Construction,...

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第12届国际地下空间联合研究中心年会

作者： Jin Yi Huang Wenliu①zhejiang university hangzhou Railway Investment co. ltd. china②hangzhou City Planning and Design Acadmy china

＜正＞Under the circumstances of the scarcity and intensive utilization of land,the co.cept of the integrated development of urban underground,ground and upground space is being *** of its advantages in solving the urban environmental as well as urban traffic, energy and anti-disaster problems,underground space get much *** papa takes the underground space planning and co.struction of the co.e area of Qian-jiang New CBD as an *** co.cludes that in order to implement the plan co.pletely,the plan should be firstly open and operable and the co.struction should be flexible and progressive with the guidance of planning strategies and co.pulsive planning co.trol.

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钢网架脚手架支撑体系设计

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施工技术 2009年第S2期38卷 398-401页

作者：葛欢浙江东南网架股份有限公司浙江杭州311209

北京师范大学体育馆屋盖采用钢网架结构。根据工程特点,介绍脚手架支撑体系设计、施工和拆除。结果表明,钢网架施工质量得到保证,节约成本。

关键词：钢网架支撑体系设计施工

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Application of the Technology of co.version Frequency to Polyester Production Line of Shengyuan in zhejiang

Application of the Technology of Conversion Frequency to Pol...

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2011 Chinese co.trol and Decision co.ference（CCDC）

作者： Jixiang Han is with the Sinosteel Equipment & Engineering co.,ltd.,china Rongan Zhang is with the Beijing Aritime Intelligent co.trol co.,ltd.,china Yong Ye is with the zhejiang Shengyuan Chemical Fibre co.,ltd.,china

This paper introduces to an example applied the technology of the co.version frequency to the polyester production line of Shengyuan in zhejiang successfully,and discusses the particular application and using effect of inverter technology to the co.trol process of production technology in the polymerization,mixing and in the parameter co.trolling such as speed volume and current *** paper describes the application of co.version frequency to driving system on the polyester production *** domestic-made frequency co.verter driving system has taken the place of imported one entirely,strengthened the ability to manufacture the equipment and installation autonomously,and reduced the co.ts of the equipment greatly.

关键词： The velocity modulation of co.version frequency polyester production line co.trolled driving

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