检索结果-南通市图书馆

Petroleum Science 2008年第4期5卷 334-339页

作者： Li Weichao Wu Xiaodong Shi Junfeng key laboratory of Petroleum Engineering Ministry of Education China University of Petroleum Beijing 102249 China

A new method based on the combination of a neural network and a genetic algorithm was proposed to rank the order of exploitation priority of coalbed methane reservoirs. The neural network was used to acquire the weights of reservoir parameters through sample training and genetic algorithm was used to optimize the initial connection weights of nerve cells in case the neural network fell into a local minimum. Additionally, subordinate functions of each parameter were established to normalize the actual values of parameters of coalbed methane reservoirs in the range between zero and unity. Eventually, evaluation values of all coalbed methane reservoirs could be obtained by using the comprehensive evaluation method, which is the basis to rank the coalbed methane reservoirs in the order of exploitation priority. The greater the evaluation value, the higher the exploitation priority. The ranking method was verified in this paper by ten exploited coalbed methane reservoirs in china. The evaluation results are in agreement with the actual exploitation cases. The method can ensure the truthfulness and credibility of the weights of parameters and avoid the subjectivity caused by experts. Furthermore, the probability of falling into local minima is reduced, because genetic the algorithm is used to optimize the neural network system.

关键词： Coalbed methane neural network system genetic algorithm evaluation index weight

Growth of marine bacteria and ammonium regeneration from substrates in different C:N ratios

在线全文

学校读者我要写书评

暂无评论

Acta Oceanologica Sinica 2009年第3期28卷 59-64页

作者： WANG Jiangtao YIN Xiaonan key laboratory of Ministry of Education for Marine Chemistry Theory and Technology OceanUniversity of China Qingdao 266003 China

Natural assemblages of marine bacteria were chosen in a batch culture experiments. The impact of varying nitrogen substrate concentrations and the substrate C：N ratios （C：Ns） on the bacterial C：N ratio （C：NB）, the bacterial growth efficiency （BGE） and ammonium regeneration was mainly examined. The C：Ns ratios varied from 5：1 （carbon limitation） to 40：1 （nitrogen limitation） with varying combinations of glucose and NO3. The C：NB ratio had positive relationship with the C：Ns ratio （r=0.93, n=8）, whose value was 3.77 when the C：Ns ratio was 5：1 but increased to 6.47 when the C：Ns ratio was 40：1. These results indicate that the C：NB ratio is a potential diagnostic tool for determining the bacterial growth in natural waters controlled by either, carbon or nitrogen. BGE decreased with the declining nitrate concentration and negatively related to C：N8 （r=-0.51, n=8）. The average value of BGE was 0.20. This value was a little lower than other reports, which could be induced by the nitrogen source used in our experiments. Finally, regeneration time of ammonium delayed with the increasing C：Ns ratio, which indicates that there were different metabolism mechanisms when bacterial growth was limited by carbon source and nitrogen source.

关键词： marine bacteria C:N bacteria growth efficiency ammonium

亚低温对脑出血大鼠神经细胞凋亡及神经功能缺损的影响

在线全文

学校读者我要写书评

暂无评论

中风与神经疾病杂志 2012年第7期29卷 618-621页

作者：党静静王志萍徐州医学院江苏省麻醉学重点实验室江苏徐州221002 南京医科大学附属无锡市第一人民医院麻醉科江苏无锡214023

目的研究延迟性亚低温对大鼠脑出血后神经细胞凋亡及神经功能缺损的动态影响。方法将大鼠随机分为正常组(N),假手术组(S),脑出血组(M),延迟12h亚低温组(HT12)和延迟24h亚低温组(HT24)。应用立体定向技术将Ⅳ型胶原酶注入大鼠尾壳核制作... 详细信息

目的研究延迟性亚低温对大鼠脑出血后神经细胞凋亡及神经功能缺损的动态影响。方法将大鼠随机分为正常组(N),假手术组(S),脑出血组(M),延迟12h亚低温组(HT12)和延迟24h亚低温组(HT24)。应用立体定向技术将Ⅳ型胶原酶注入大鼠尾壳核制作脑出血模型,模型制备后于12h及24h点将大鼠放入冷室降温,维持肛温在(33±1)℃,均持续12h,各组在模型制作后12h、24h、36h、3d、7d记录大鼠神经功能缺损评分及死亡率。大鼠脑组织做HE染色及TUNEL染色,流式细胞技术观察神经细胞凋亡数目的变化。结果亚低温(HT12,HT24)可减少脑出血后大鼠各时间点的TUNEL染色的阳性细胞数(P3d时亚低温组(HT12,HT24)神经细胞凋亡率及大鼠7d的死亡率明显降低(P0.05)。结论延迟的亚低温治疗可减少大鼠脑出血后神经细胞的凋亡,降低死亡率,发挥脑保护作用。

关键词：脑出血亚低温凋亡神经功能缺损

Changes in hippocampal neurons and memory function during the developmental stage of newborn rats with hypoxic-ischemic encephalopathy

在线全文

学校读者我要写书评

暂无评论

Neural regeneration Research 2006年第8期1卷 681-684页

作者： Chuanjun Liu1, Yue Li2, Huiying Gao3 1Department of Pediatric Internal Medicine, Taian Health Center for Women and Children,Taian 271000, Shandong province, china 2Department of Anatomy, 3Department of Histology and Embryology, Taishan Medical College, Taian 271000, Shandong province, china Department of Pediatric Internal Medicine Taian Health Center for Women and Children Taian 271000 Shandong Province China Department of Anatomy Taishan Medical College Taian 271000 Shandong Province China Department of Histology and Embryology Taishan Medical College Taian 271000 Shandong Province China

BACKGROUND: Under the normal circumstance, there exist some synapses with inactive functions in central nervous system (CNS), but these functions are activated following nerve injury. At the early stage of brain injury, the abnormal functions of brain are varied, and they have very strong plasticity and are corrected easily. OBJECTIVE: To observe the changes of neuronal morphology in hippocampal CA1 region and memory function in newborn rats with hypoxic-ischemic encephalopathy(HIE) from ischemia 6 hours to adult. DESIGN: Completely randomized grouping, controlled experiment. SETTING: Taian Health Center for Women and Children; Taishan Medical College. MATERIALS: Altogether 120 seven-day-old Wistar rats, of clean grade, were provided by the Experimental Animal Center, Shandong University of Traditional Chinese Medicine. Synaptophysin (SYN) polyclonal antibody was provided by Maixin Biological Company, Fuzhou. METHODS: This experiment was carried out in the laboratory of Morphology, Taishan Medical College between October 2000 and December 2003. ① The newborn rats were randomly divided into 2 groups: model group and control group, 60 rats in each group. Five rats were chosen from each group at postoperative 6 hours, 24 hours, 72 hours, 7 days, 2 weeks and 3 weeks separately for immunohistochemical staining. Fifteen newborn rats were chosen from each group at postoperative 4 weeks and 2 months separately for testing memory ability (After test, 5 rats from each group were sacrificed and used for immunohistochemical staining)② The right common carotid artery of newborn rats of model group was ligated under the anesthetized status. After two hours of incubation, the rats were placed for 2 hours in a container filled with nitrogen oxygen atmosphere containing 0.08 volume fraction of oxygen, thus, HIE models were created; As for the newborn rats in the control group, only blood vessels were isolated, and they were not ligated and hypoxia-treated. ③ Thalamencephal tissue se

关键词： Changes in hippocampal neurons and memory function during the developmental stage of newborn rats with hypoxic-ischemic encephalopathy

Ectopic Neurogenesis in Forebrain Cholinergic System-Related Areas of Rat Dementia Model

在线全文

学校读者我要写书评

暂无评论

Ectopic Neurogenesis in Forebrain Cholinergic System-Related...

Expression of nitric oxide synthase in the spinal cord after selective brachial plexus injury

“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Xinhua Zhang~1,Guohua Jin~1,Wei Li~1,Linqing Zou~1,Jinhong Shi~1,Jianbing Qin~1,Meiling Tian~1,Haoming Li~1 1.Department of Anatomy and Neurobiology,jiangsu key laboratory of Neurorcgencration,nantong University,19 Qixiu Road, nantong City,china

Lesions to the fimbria fornix(FiFx) plus cingulate bundle(CB),the principal routes of communication of forebrain cholinergic regions,produce lasting impairment of spatial learning and memory in *** report that extensive neurogenesis takes place in the FiFx,CB and basalis magnocellularis(MBN) following FiFx plus CB *** revealed that nestin-expressing cells were present in all three areas following lesion;the majority of nestin-positive cells were also positive for 5-bromo-2-deoxy-uridine(BrdU) incorporation,a marker of DNA synthesis. Nestin-positive proliferative cells were almost entirely absent from unlesioned *** cultured in vitro from lesioned FiFx displayed the characteristics of neural stem cells(NSCs) - proliferation, expression of embryonic markers,and multipotential differentiation into neurons,astrocytes and *** early stages after transection a small number of immature and migrating doublecortin (DCX)-immunopositive neurons were detected in lesioned FiFx where neuronal cell bodies are normally *** later stages post-lesion immature neurons developed into -tubulin Ill-positive mature neurons. Lentivirus labeling assay implied that the injury-induced neurogenesis in FiFx may be from local neurogenic astrocytes but not from dentate *** results demonstrate that insult to cholinergic tracts can stimulate NSC proliferation and neuronal regeneration not only in innervated regions but also in the projection pathways *** neurogenesis in cholinergic system-related areas provides an additional mechanism for repair of cholinergic innervation following damage.

关键词： Neural stemcell neurogenesis fimbira fornix

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Neural regeneration Research 2006年第9期1卷 773-776页

作者： Na Liu1, Feng Li1, Longju Chen2, Wutian Wu3 1Medical College, Foshan University, Foshan 528000, Guangdong province, china Master,Assistant, 2Department of Anatomy, Yunyang Medical College, Shiyan 442000, Hubei province, china 3Department of Anatomy, Medical College of Hongkong University, Hongkong 999077, china Medical College Foshan University Foshan 528000 Guangdong Province China Department of Anatomy Yunyang Medical College Shiyan 442000 Hubei Province China Department of Anatomy Medical College Hongkong University Hong Kong 999077 Hong Kong

BACKGROUND: Some researches showed that motoneurons in spinal cord anterior horn wound die following brachial plexus injury, but the concrete mechanism of motoneurons death remains unclear. OBJECTIVE: To observe the expression of nitric oxide synthase (NOS) and survival of C7 motoneurons in spinal cord of rats after selective brachial plexus injury. DESIGN: A randomized controlled animal experiment. SETTING:Department of Anatomy, Sun Yet-sen Medical College, Sun Yet-sen University. MATERIALS: Totally 35 adult healthy male Sprague-Dawley rats with the body mass of 200-300 g were provided by Experimental Animal Center, Sun Yet-sen Medical College, Sun Yat-sen University. The rats were divided into control group (n =5) and experimental group(n =30) by random number table method, and the experimental group was divided into three injury subgroups: anterior root avulsion group, dorsal root transection group and spinal cord hemisection group, 10 rats in each group. There were horse anti-neuronal NOS (nNOS) polycolonal antibody (Sigma company) and nicotina mideadeninedinucleotide phosphate (NADPH-d) (Sigma Company). METHODS: The experiment was performed at Department of Anatomy, Sun Yet-sen Medical College, Sun Yet-sen University between September 2004 and April 2005. ①After anesthetizing the rats, the spinous process of second thoracic vertebra as a marker, the vertebra was exposed from C5 to T1 and the lamina of vertebra was unclenched, and spinal dura mater was carved to expose the spinal nerve dorsal roots of C5-T1. The right ventral root of C7 was avulsed, and the residual root was removed in anterior root avulsion group. The right ventral root of C7 was avulsed and the right dorsal roots of brachial plexus (C5-T1) were cut off in dorsal root transection group. In spinal cord hemisection group, the hemisection between the C5 and C6 spinal segment on right side and avulsion of right ventral root of C7 were made. In the control group, the vertebra from C5 to T1 was uncle

关键词： Expression of nitric oxide synthase in the spinal cord after selective brachial plexus injury NOS

Correlative study on the cognitive dysfunction of patients with myasthenia gravis

在线全文

学校读者我要写书评

暂无评论

Neural regeneration Research 2006年第8期1卷 744-746页

作者： Wenli Chen1, Wenmin Wang2, Yi Wang3 1Department of Neurology, Yunnan First People’s Hospital, Kunming 650032, Yunnan province, china 2Department of Neurology, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan province, china 3Department of Neurology, Huashan Hospital Affiliated to Shanghai Fudan University, Shanghai 200040, china Department of Neurology Yunnan First People's Hospital Kunming 650032 Yunnan Province China Department of Neurology Hospital of Kunming Medical College Kunming 650032 Yunnan Province China Department of Neurology Huashan Hospital Shanghai Fudan University Shanghai 200040 China

BACKGROUND: Myasthenia gravis (MG) is considered as a peripheral neuromuscular disorder. Some investigations demonstrate that MG affects central nervous system (CNS), and there are disorders in cognitive function, affection, sleep, mental status and others; however, there are also some different standpoints. Some questions are still disputed, such as whether or not MG exists in the CNS and cognitive function is impaired. DATA SOURCES: Using the terms of 'myasthenia gravis, neuropsychological test, memory, central, brain, cognitive dysfunction and so on', we searched the Medline database for the articles about CNS involved in MG, nerve and neuropsychology as well as cognitive function, which were published in the English language between January 1985 and December 2004. Meanwhile, we also searched CNKI database by inputting 'myasthenia gravis, neuropsychology and cognitive function' in Chinese for articles about involved neuropsychology and cognitive function which were published between October 1995 and June 2006 in the Chinese language. STUDY SELECTION: Data were checked in the first trial, and articles about the influences of MG on CNS, neuropsychology, cognitive function and central cholinergic function were selected, then those were obviously unrelated with above criteria were excluded. Articles which expound the influences of MG on neuropsychology and cognitive were included, but those with repeated contents were also excluded. DATA EXTRACTION: A total of 89 related English articles and 34 Chinese articles were collected, including 43 studies on involved CNS and 32 studies on involved central cholinergic function and cholinergic receptor. Sixty-three literatures were about neuropsychology and cognitive function, which were closely related with this article. Seventy-two articles met inclusive criteria and 66 were excluded. Thirty of seventy-two were chosen for analysis. DATA SYNTHESIS: MG is an autoimmune disease of muscular diseased fatigue induced by transmissi

关键词： cognitive function central nervous system cholinergic receptor autoimmune disease English language Chinese language used in

在线全文

学校读者我要写书评

暂无评论

哺乳动物心肌细胞再生专家共识

生理学报 2024年第2期76卷 175-214页

作者：中国生理学会整合生理学专业委员会老年心脑血管病教育部重点实验室李良鹏曾春雨杨黄恬管又飞不详第三军医大学大坪医院中国科学院上海营养与健康研究所大连医科大学

心肌梗死引起的大量心肌细胞损失导致心脏病理性重构和心力衰竭的发生,如何促进心肌再生是修复受损心脏的关键问题。心肌细胞再生主要源于原有心肌细胞的增殖已被广泛认同。近年来,该领域的快速发展加深了我们对心肌细胞再生的内源性细... 详细信息

心肌梗死引起的大量心肌细胞损失导致心脏病理性重构和心力衰竭的发生,如何促进心肌再生是修复受损心脏的关键问题。心肌细胞再生主要源于原有心肌细胞的增殖已被广泛认同。近年来,该领域的快速发展加深了我们对心肌细胞再生的内源性细胞和微环境特征、外源性影响因素、分子生物学机制以及干预策略等各方面的认识。因此,我们对哺乳动物心肌细胞再生的定义、特征、评价和研究方法、调控机制以及干预措施等进行整理并形成共识,旨在明确该领域尚需进一步阐明的重要问题,推动心肌再生研究的深化及其在临床上的应用转化。

关键词：心肌梗死心力衰竭心脏重塑心肌再生心肌细胞增殖

同方期刊数据库

Studies on Genetic Transformatiom of Embryogenic Suspension Cultures of Sweetpotato

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in china 2003年第7期2卷 791-797页

作者： ZHAI Hong and LIU Qing-chang(key laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/china Agricultural University , Beijing 100094 , P.R. china) key laboratory of Crop Genomics and Genetic Improvement Ministry of Agriculture/China Agricultural University Beijing 100094 P. R. China

Genetic transformation of embryogenic suspension cultures of sweetpotato cv. Lizixiang was conducted by using Agrobacterium tumefaciens strain A208SE harboring the binary vectors pROA93 with β-glucronidase(GUS)and neomycin phosphotransferase(NPTⅡ)genes. The results indicated that embryogenic suspension cultures precultured for 1 - 3 d were suitable for the transformation. The optimal cocultivation time was 4 - 5 d. The optimal concentration of kanamycin was 50-75 mg L-1 for suspension culture and 100 mg L-1 for embryogenic callus proliferation and plant regeneration. The optimal concentration of carbencillin was 100 mg L-1. Transgenic plants identified with GUS assays and PCR analyses were obtained.

关键词： Sweetpotato Embryogenic suspension cultures A. tumefaciens-mediated method Transge-nic plants

Expression of SOCS3 throughout liver regeneration is not regulated by DNA methylation

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2012年第4期11卷 401-406页

作者： Gang Guo,Jie Xia,Ji Bao,Huai-Qiang Sun,Yu-Jun Shi and Hong Bu key laboratory of Transplant Engineering and Immunology,Ministry of Health and Department of Pathology,West china Hospital,Sichuan University,Chengdu 610041,china [a] key laboratory of Transplant Engineering and Immunology Ministry of Health West China Hospital Sichuan University Chengdu 610041 China [b] key laboratory of Transplant Engineering and Department of Pathology West China Hospital Sichuan University Chengdu 610041 China

BACKGROUND:While suppressor of cytokine signaling 3(SOCS3) plays a crucial role in suppressing dysplasia and tumorigenesis,it also offers a typical instance of DNA methylation in the regulation of gene transcription,since the promoter region of the SOCS3 gene is rich in CpG islands(CGIs).During liver regeneration initiated by partial hepatectomy,SOCS3 acts as a suppressor to balance the acute-phase response and terminate the *** study aimed to determine whether the variation of SOCS3 expression throughout liver regeneration is also regulated by its DNA ***:We established a 70% partial hepatectomy mouse model and the animals were sacrificed at indicated times to assess the SOCS3 *** performed bisulfite sequencing PCR and DNA sequencing to investigate the detailed cytosine methylation in the SOCS3 ***:Within the promoter sequence,58 CGIs were identified and 30 were found variously methylated before or after operation;however,methylation remained at a very low *** evidence indicated that the total methylation level or the methylation of any CpG site regularly changed throughout liver ***:DNA methylation or demethylation seems to be a relatively stable modification of cytosine,but not a dynamic and reversible process to regulate gene transcription in daily and acute pathophysiological events.

关键词： liver regeneration suppressor of cytokine signaling 3 DNA methylation