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Effect of calcitonin gene-related peptide and nerve growth factor on spatial learning and memory abilities of rats following focal cerebral ischemia/reperfusion

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Neural Regeneration Research 2006年第8期1卷 673-676页

作者： Guangshun Zheng1, Yongjie Yang2, Xiubin Fang3 1department of Neurosurgery, Second Hospital of Xiamen, Xiamen 361021, Fujian province, china 2department of Neurosurgery, Second Hospital Affiliated to china medical university, shenyang 110004, liaoning province, china 3department of neurobiology, basic medical college of china medical university, shenyang 110001, liaoning province, china department of Neurosurgery Second Hospital of Xiamen Xiamen 361021 Fujian Province China department of Neurosurgery China Medical University Shenyang 110004 Liaoning Province China department of neurobiology Basic Medical College China Medical University Shenyang 110001 Liaoning Province China

BACKGROUND: Calcitonin gene-related peptide (CGRP) and nerve growth actor (NGF) cam improve spatial learning and memory abilities of rats with cerebral ischemia/reperfusion; however, the effect of combination of them on relieving learning and memory injury following cerebral ischemia/reperfusion should be further studied. OBJECTIVE: To study the effects of exogenous CGRP and NGF on learning and memory abilities of rats with focal cerebral ischemia/reperfusion. DESIGN: Randomized controlled animal study. SETTING: department of Neurosurgery, the Second Hospital of Xiamen; department of Neurosurgery, the Second Affiliated Hospital of china medical university; department of neurobiology, basic medical college of china medical university. MATERIALS: A total of 30 healthy male SD rats, aged 8 weeks, of clean grade, weighing 250-300 g, were provided by Experimental Animal department of china medical university. All rats were randomly divided into sham-operation group, ischemia/reperfusion group and treatment group with 10 in each group. The main reagents were detailed as the follows: 100 g/L chloral hydrate, 0.5 mL CGRP (2 mg/L, Sigma Company, USA), and NGF (1× 106 U/L, 0.5 mL, Siweite Company, Dalian). METHODS: The experiment was carried out in the department of neurobiology, basic medical college of china medical university from February to July 2005. Rat models of middle cerebral artery occlusion were established by method of occlusion, 2 hours after that rats were anesthetized and the thread was slightly drawn out for 10 mm under direct staring to perform reperfusion. Rats in the ischemia/reperfusion group received intraperitoneal injection of 1 mL saline via the abdomen at two hours later, while rats in the treatment group at 2 hours later received intraperitoneal injection of 2 mg/L CGRP (0.5 mL) and 1×106 U/L NGF (0.5 mL) once a day for 10 successive days. First administration was accomplished within 15 minutes after ischemia/reperfusion. Rats in the sham-operation

关键词： Effect of calcitonin gene-related peptide and nerve growth factor on spatial learning and memory abilities of rats following focal cerebral ischemia/reperfusion CGRP MCAO gene

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Cyclooxygenase-2 polymorphisms and susceptibility to gastric carcinoma: A meta-analysis

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World Journal of Gastroenterology 2010年第43期16卷 5510-5517页

作者： Jia-Li Liu, Zhen-Ning Wang, department of Surgical Oncology and General Surgery, the First Affiliated Hospital of china medical university, shenyang 110001, liaoning province, china Yuan Liang, department of Oncology, the Shengjing Affiliated Hospital of china medical university, shenyang 110001, liaoning province, china Xin Zhou, department of Obstetrics and Gynecology, the Shengjing Affiliated Hospital of china medical university, shenyang 110001, liaoning province, china Li-Li Xing, department of Oncology, the Fourth Affiliated Hospital of china medical university, shenyang 110032, liaoning province, china department of Surgical Oncology and General Surgery the First Affiliated Hospital of China Medical University Shenyang 110001 Liaoning Province China. josieon826@***

AIM: To investigate the association of the cyclooxygenases-2 (COX-2) polymorphisms and susceptibility to gastric cancer (GC) by means of meta-analysis. METHODS: Publications addressing the association between polymorphisms of COX-2 and susceptibility to GC were selected from the MEDLINE, EMBASE and CBMdisc databases. Data was extracted from the studies by 2 independent reviewers. The meta-analyses were performed by RevMan 5.0.23. From these data, odds ratio (OR) with 95% confidence interval (CI) was ***: Ten studies were retrieved reporting a total of 11 COX-2 polymorphisms. Carriers of -765C, -1195A, -1290G, *2430T alleles and *429TT genotype revealed increased risk for GC (OR = 1.71, 95% CI: 1.01-2.90, P = 0.05; OR = 1.58, 95% CI: 1.05-2.38, P = 0.03; OR = 1.55, 95% CI: 1.01-2.39, P = 0.05; OR = 2.62, 95% CI: 1.20-5.73, P = 0.02 and OR = 0.74, 95% CI: 0.59-0.95, P = 0.02, respectively). CONCLUSION: The -765C, -1195A, -1290G, *2430T alleles and *429TT genotype of COX-2 polymorphisms were determined a significant association with susceptibility to GC.

关键词： Cyclooxygenases-2 Gastric cancer Polymorphisms Susceptibility Meta-analysis

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Influence of rotating magnetic field on cerebral infarction volume, cerebral edema and free radicals metabolism after cerebral ischemia/reperfusion injury in rats

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Neural Regeneration Research 2006年第9期1卷 777-780页

作者： Xiaohong Liu1, Zhiqiang Zhang2, Lixin Zhang3 1liaoning Disabled Children’s Rehabilitation Center, shenyang 110015, liaoning province, china 2department of Rehabilitation, Second Hospital Affiliated to china medical university, shenyang 110003, liaoning province, china 3department of Rehabilitation and Physiotherapy, First Hospital Affiliated to china medical university, shenyang 110001, liaoning province, china liaoning Disabled Children's Rehabilitation Center Shenyang 110015 Liaoning Province China department of Rehabilitation China Medical University Shenyang 110003 Liaoning Province China department of Rehabilitation and Physiotherapy China Medical University Shenyang 110001 Liaoning Province China

BACKGROUND: It has shown that magnetic field can improve blood circulation, decrease blood viscosity, inhibit free radicals, affect Ca2+ flow in nerve cells, control inflammatory and immunological reaction, and accelerate nerve cell regeneration. In addition, protective effect of magnetic field, which acts as an iatrophysics, on ischemic brain tissues has been understood gradually. OBJECTIVE: To investigate the effects of rotating magnetic field (RMF) on volume of cerebral infarction, cerebral edema and metabolism of free radicals in rats after cerebral ischemia/reperfusion injury. DESIGN: Randomized controlled animal study. SETTING: Rehabilitation Center of disabled children, Liaoniang; department of Rehabilitation, the Second Affiliated Hospital, china medical university; department of Rehabilitation Physiotherapy, the First Affiliated Hospital, china medical university. MATERIALS: A total of 70 healthy Wistar rats aged 18-20 weeks of both genders were selected and randomly divided into 3 groups: sham operation group with 12 rats, control group with 20 rats and treatment group with 38 rats. The treatment group included 4 time points: immediate reperfusion with 6 ones, 6-hour reperfusion with 20 ones, 12-hour reperfusion with 6 ones and 18-hour reperfusion with 6 rats. Main instruments were detailed as follows: magnetic head of rotating magnetic device was 6 cm in diameter; magnetic induction intensity at the surface of magnetic head was 0.25 T in silence; the maximal magnetic induction intensity was 0.09 T at the phase of rotation; the average rotating speed was 2500 r per minute. METHODS: The experiment was carried out in the china medical university in March 2003. Focal cerebral ischemic animal models were established with modified Longa’s method. Operation was the same in the sham operation, but the thread was inserted as 10 mm. Neurologic impairment was assessed with 5-rating method to screen out cases. Those survivals with grade 1 and grade 2 after ischemia f

关键词： cerebral edema and free radicals metabolism after cerebral ischemia/reperfusion injury in rats free

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Controlling N-methyl-D-aspartate receptor subunit 1 with calcitonin gene related peptide after cerebral ischemic injury

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Neural Regeneration Research 2006年第7期1卷 585-588页

作者： Jixiang Cui1, Peng Qu2, Chunping Qiao3 1department of Clinical Laboratory, Fourth Affiliated Hospital of china medical university, shenyang 110032, liaoning province, china 2department of neurobiology, basic medical college of china medical university, shenyang 110032, liaoning province, china 3department of Emergency, Baicheng Central Hospital of Jilin province, Baicheng 137000, Jilin province, china department of Clinical Laboratory Hospital of China Medical University Shenyang 110032 Liaoning Province China department of neurobiology Basic Medical College China Medical University Shenyang 110032 Liaoning Province China department of Emergency Baicheng Central Hospital of Jilin Province Baicheng 137000 Jilin Province China

BACKGROUND: Activation of N-methyl-D-aspartate receptor (NMDAR) is a key link of exitotoxicity at the phase of cerebral ischemic injury. Because NMDAR is a main way to mediate internal flow of Ca2+ among glutamic acid receptors, over-excitation can cause neuronal apoptosis. Calcitonin gene related peptide has a strongly biological activity. On one hand, it can protect ischemic neurons through inhibiting the expression of NMDAR1 mRNA; on the other hand, it can play the protective effect through down-regulating the expression of NMDAR1 mRNA by exogenous calcitonin gene related peptide. OBJECTIVE: To observe the expression of NMDAR1 and the regulatory effect of calcitonin gene related peptide on the expression of NMDAR1 mRNA and protein in the cerebral cortex of rats with focal cerebral ischemia/reperfusion (I/R). DESIGN: Randomized controlled animal study. SETTING: china medical university. MATERIALS: A total of 216 healthy male Wistar rats, general grade, weighing 250-280 g, were selected in this study. Twelve rats were randomly selected to regard as control group; meanwhile, other 204 rats were used to establish middle cerebral artery occlusion/reperfusion (MACO) models. The main reagents were detailed as follows: calcitonin gene related peptide (Sigma Company); calcitonin gene related peptide kit (Boster Company); antibody Ⅰ, Ⅱ and antibody β-actin Ⅰ, Ⅱ of NMDAR1 mRNA and chemiluminescence reagent (Santa Cruz Company, USA). METHODS: The experiment was carried out in the Laboratory of neurobiology of china medical university from August 2005 to June 2006. ① Right MCAO models of rats were established to cause focal ischemia and scored based on Zea Longa five-grade scale. If the scores were 1, 2 and 3 after wakefulness, the MACO models were established successfully and involved in the experiment. A total of 120 rats with successful modeling were randomly divided into I/R group and administration group with 60 in each group. All rats in the both groups were observed at

关键词： NMDAR mRNA Controlling N-methyl-D-aspartate receptor subunit 1 with calcitonin gene related peptide after cerebral ischemic injury gene

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Decreased IgA+ plasma cells and IgA expression in acute liver necrosis mice

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World Journal of Gastroenterology 2010年第30期16卷 3827-3833页

作者： Jin-Long Fu, Zhao-Han Wang, Guo-Zhen Li, Yu-Rong Wang, Pei Liu, department of Infectious Diseases, The First Aff iliated Hospital, china medical university, shenyang 110001, liaoning province, china department of Infectious Diseases The First Affiliated Hospital China Medical University No. 155 Nanjing Street Shenyang 110001 Liaoning Province China. syliupei2003@***

AIM: To investigate the number of intestinal immunoglobulin A (IgA+) plasma cells and expression of intestinal IgA in mice with acute liver necrosis. METHODS: A model of acute liver necrosis was established by intraperitoneal injection of galactosamine (GalN) and lipopolysaccharide (LPS). Sixty mice were randomly divided into one of 4 equal groups: normal control, acute liver necrosis, LPS, or GalN. Hematoxylin and eosin staining, immunohistochemistry, and an enzyme-linked immunosorbent assay were employed to assess liver and intestinal injury, count intestinal IgA+ plasma cells, and measure the expression level of IgA and interferon γ (IFN-γ) in the small intestinal mucosa of mice. RESULTS: Injured intestinal mucosa was observed in the acute liver necrosis group but not in the normal, LPS or GalN groups. Compared with the normal group,intestinal IgA+ plasma cells were slightly decreased in the LPS and GalN groups [429 ± 20 per high power f ield (HPF), 406 ± 18/HPF, respectively], whereas they were markedly decreased in the acute liver necrosis group (282 ± 17/HPF vs 495 ± 26/HPF in normal group, P 0.05). CONCLUSION: Intestinal IgA+ plasma cells and IgA expression levels indicating that mucosal immune barrier dysfunction, does exist in acute liver necrosis.

关键词： Acute liver necrosis Intestinal mucosa Immunity Immunoglobulin A

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Hepatic blood inflow occlusion without hemihepatic artery control in treatment of hepatocellular carcinoma

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World Journal of Gastroenterology 2010年第46期16卷 5895-5900页

作者： Shan Jin,department of General Surgery,Affiliated Hospital of Inner Mongolia medical college,Hohhot 010050,Inner Mongolia Autonomous Region,china Chao-Liu Dai,department of Hepatobiliary Surgery,Shengjing Hospital,china medical university,shenyang,liaoning province,china department of General Surgery Affiliated Hospital of Inner Mongolia Medical College Hohhot 010050 Inner Mongolia Autonomous Region China. jinshangood@***

AIM:To investigate the clinical significance of hepatic blood inflow occlusion without hemihepatic artery control (BIOwHAC) in the treatment of hepatocellular carcinoma (HCC).METHODS:Fifty-nine patients with HCC were divided into 3 groups based on the technique used for achieving hepatic vascular occlusion:group 1,vascular occlusion was achieved by the Pringle maneuver (n=20);group 2,by hemihepatic vascular occlusion (HVO) (n=20);and group 3,by BIOwHAC (n=19).We compared the procedures among the three groups in term of operation time,intraoperative bleeding,postoperative liver function,postoperative complications,and length of hospital ***:There were no statistically significant differences (P > 0.05) in age,sex,pathological diagnosis,preoperative Child's disease grade,hepatic function,and tumor size among the three *** intraoperative complications or deaths occurrred,and there were no significant intergroup differences (P > 0.05) in intraoperative bleeding,hepatic function change 3 and 7 d after operation,the incidence of complications,and length of hospital *** and Pringle maneuver required a significantly shorter operation time than HVO;the difference in the serum alanine aminotransferase or aspartate aminotransferase levels before and 1 d after operation was more significant in the BIOwHAC and HVO groups than in the Pringle maneuver group (P < 0.05).CONCLUSION:BIOwHAC is convenient and safe;this technique causes slight hepatic ischemia-reperfusion injury similar to HVO.

关键词： Hepatic blood inflow occlusion without hemihepatic artery control Hepatocellular carcinoma Intraoperative bleeding Ischemia-reperfusion injury

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Corticosteroids or non-corticosteroids: a fresh perspective on alcoholic hepatitis treatment

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Hepatobiliary & Pancreatic Diseases International 2011年第5期10卷 458-464页

作者： Fei Wang and Bing-Yuan Wang department of Gastroenterology, First Affiliated Hospital, china medical university, shenyang 110001, china department of Gastroenterology First Affiliated Hospital China Medical University Shenyang 110001 China

BACKGROUND: Alcoholic hepatitis (AH) is a necrotizing inflammatory process caused by alcoholic liver injury. It carries a significant short-term mortality. The management of AH is challenging. Although corticosteroids have been demonstrated to exert anti-inflammatory and antifibrotic effects, their efficacy for the treatment of AH remains debatable. DATA SOURCES: A literature search was performed of MEDLINE, ScienceDirect, SpringerLink and Wiley InterScience using the key words 'alcoholic hepatitis', 'alcoholic liver disease', and 'corticosteroids'. The available data reported in the relevant literature were analyzed. RESULTS: More than 17 controlled trials and at least 13 meta-analyses have reported the efficacy of corticosteroids in the treatment of AH in the past 40 years. Many were poorly designed and used different inclusion/exclusion criteria, making it difficult to reach a consensus. In this review, we summarized all the controversial data in the past decade and analyzed the potential causes for the varying therapeutic effects of corticosteroids in AH. The focus of the controversy has changed from 'whether steroids are beneficial or harmful for AH patients' to 'how to accurately identify responders to steroids early and rationalize corticosteroid treatment'. An early response to glucocorticoids, as determined by calculating the Lille score after 7 days of treatment, has been shown to be a clinically useful indicator. Moreover, down-regulation of steroid sensitivity, risk of infection, and a rational therapeutic strategy of corticosteroids in AH patients are all crucial for therapeutic ***: An early and accurate determination of steroid sensitivity is important. Besides, we need to overcome the down-regulation of steroid sensitivity, reduce the infection risk and rationalize the therapeutic strategy of corticosteroids. A fresh perspective is needed on the use of corticosteroids in AH patients.

关键词： corticosteroids alcoholic liver disease alcoholic hepatitis steroid sensitivity scoring system

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Expert consensus on radiotherapy combined with immunotherapy for esophageal cancer(2024 edition)

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Radiation Medicine and Protection 2024年第2期5卷 59-67页

作者： Professional Committee of Radiation Oncology china Anti-Cancer Association Branch of Radiation Oncology Chinese medical Association Branch of Radiation Oncology TreatmentPhysician Chinese medical Doctor Association Qingsong Pang Jun Wang Zhouguang Hui Wencheng Zhang 不详 Tianjin medical university Cancer Institute&Hospital Tianjin 300060China the Fourth Hospital of Hebei medical university Shijiazhuang 050011China National Cancer Center National Clinical Research Center for CancerCancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Tianjin medical university Cancer Hospital China

Radiotherapy represents an essential treatment approach for esophageal *** recent years,immunotherapy combined with chemotherapy has become the first-line standard treatment for patients with advanced esophageal *** phase III studies on immunotherapy combined with radiotherapy for locally advanced esophageal cancer are currently *** evidence-based medical data are urgently needed to support the integration of immunotherapy and chemoradiotherapy as a new treatment strategy for patients with locally advanced esophageal *** consensus,formulated based on the latest study results,in-depth research,and thorough discussions,provides a comprehensive set of *** document extensively covers treatment strategies and evaluation methods for radiotherapy combined with immunotherapy across patients with operable esophageal cancer,inoperable locally advanced esophageal cancer,and advanced esophageal ***,common complications and radiation-related issues associated with radiotherapy combined with immunotherapy are discussed,serving as clinical *** expert group comprised members from the Professional Committee of Radiation Oncology,china Anti-Cancer Association,the Branch of Radiation Oncology,Chinese medical Association,and the Branch of Radiation Oncology Treatment Physician,Chinese medical Doctor Association.

关键词： Esophageal squamous cell carcinoma Esophageal adenocarcinoma Immunotherapy Chemoradiotherapy Radiotherapy Expert consensus

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Research progress in muscle-derived stem cells Literature retrieval results based on international database

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Neural Regeneration Research 2012年第10期7卷 784-791页

作者： Li Zhang, Wei Wang Jinzhou Clinical college of liaoning medical university Jinzhou Central Hospital, Jinzhou 121000, liaoning province, china Jinzhou Clinical college of liaoning medical university Jinzhou Central Hospital Jinzhou 121000 Liaoning Province China.

OBJECTIVE： To identify global research trends of muscle-derived stem cells （MDSCs） using a bibliometric analysis of the Web of Science, Research Portfolio Online Reporting Tools of the National Institutes of Health （NIH）, and the Clinical Trials registry database （***）. DATA RETRIEVAL： We performed a bibliometric analysis of data retrievals for MDSCs from 2002 to 2011 using the Web of Science, NIH, and ***. SELECTION CRITERIA： Inclusion criteria：（1） Web of Science：（a） peer-reviewed articles on MDSCs that were published and indexed in the Web of Science. （b） Type of articles： original research articles, reviews, meeting abstracts, proceedings papers, book chapters, editorial material and news items. （c） Year of publication： 2002-2011. （d） Citation databases： Science Citation Index-Expanded （SCI-E）, 1899-present; Conference Proceedings Citation Index-Science （CPCI-S）, 1991-present; Book Citation Index-Science （BKCI-S）, 2005-present. （2） NIH：（a） Projects on MDSCs supported by the NIH. （b） Fiscal year： 1988-present. （3） ***： All clinical trials relating to MDSCs were searched in this database. Exclusion criteria：（1） Web of Science：（a） Articles that required manual searching or telephone access. （b） We excluded documents that were not published in the public domain. （c） We excluded a number of corrected papers from the total number of articles. （d） We excluded articles from the following databases： Social Sciences Citation Index （SSCI）, 1898-present; Arts ＆ Humanities Citation Index （A＆HCI）, 1975-present; Conference Proceedings Citation Index - Social Science ＆ Humanities （CPCI-SSH）, 1991-present; Book Citation Index - Social Sciences ＆ Humanities （BKCI-SSH）, 2005-present; Current Chemical Reactions （CCR-EXPANDED）, 1985-present; Index Chemicus （IC）, 1993-present. （2） NIH：（a） We excluded publications related to MDSCs that were supported by the NIH. （b） We limited the keyword search to studies that included MDSCs within th

关键词： stem cells muscle-derived regeneration Web of Science NIH Clinical Trials bibliometric

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Optical coherence tomography assessed retinal nerve fiber layer thickness in patients with Alzheimer's disease:a meta-analysis

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International Journal of Ophthalmology(English edition) 2012年第3期5卷 401-405页

作者： Xue-Fei He 1,Yi-Ting Liu 2,Cheng Peng 1,Fan Zhang 1,Shi Zhuang 1,Jin-Song Zhang 1 1Key Laboratory of Lens Research liaoning province,department of Ophthalmology,Eye Hospital of china medical university,the Fourth Affiliated Hospital of china medical university shenyang 110005,liaoning province,china 2 department of Clinical Epidemiology,the First Affiliated Hospital,china medical university,shenyang 110001,liaoning province,china Key Laboratory of Lens Research liaoning province Department of Ophthalmology Eye Hospital of China Medical University the Fourth Affiliated Hospital of China Medical University Shenyang 110005 Liaoning Province China department of Clinical Epidemiology the First Affiliated Hospital China Medical University Shenyang 110001 Liaoning Province China

AIM:To investigate the difference of retinal nerve fiber layer(RNFL) thickness between Alzheimer's disease patients and normal people,so as to provide clue for the early diagnosis of Alzheimer's disease.· METHODS:The articles on the association of RNFL thickness and Alzheimer's disease were retrieved by searching international and national *** qualified articles were assessed by meta analysis with Stata11.0 *** results were pooled using weighted mean difference(WMD) with a corresponding 95% confidence interval(CI).· RESULTS:Totally 7 studies enrolled 324 eyes were included in the *** results of meta analysis showed that in AD patients,there was a significant average RNFL thickness reduction compared with the control group [WMD=-17.561,95%CI:(-23.971,-11.151)].There were significant differences in superior,inferior,nasal and temporal RNFL thickness between the two *** with a 95%CI were [-18.829,95%CI:(-25.915,-11.743);P34.304,-17.247);P 3);Pbasically symmetrical,indicating that there was no publication bias existed.· CONCLUSION:There are significant differences in the RNFL thickness in all quadrants between the two *** thickness is reduced in AD patients compared with the control group.·

关键词： retinal nerve fiber layer thickness optical coherence tomography Alzheimer's disease meta analysis

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