检索结果-南通市图书馆

Nitrite-derived nitric oxide by xanthine oxidoreductase protects the liver against ischemia- reperfusion injury

Hepatobiliary & Pancreatic Diseases International 2005年第3期4卷 350-355页

作者： department of general surgery ( Lu P, Wang CY and Chen DD), and department of Radiology (Liu F), union hospital, tongji medical college, huazhong university of science and technology, wuhan 430022, china Cold Spring Harbor Laboratory, New York 11724, USA ( Yao Z) general surgery Laboratory, union hospital, tongji medical college , huazhong university of science and technology, wuhan 430022 , china (Tian Y, Zhang JH and Wu YH) department of general surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China. luliu@***

It was demonstrated that xanthine oxidoreductase (XOR), during ischemia, catalyzes the formation of nitric oxide (NO) from nitrite (NO_2^-) and this NO_2^--derived NO protects the isolated perfused rat heart against the damaging effects of ischemia-reperfusion (I/R) when conventional nitric oxide synthase (NOS) -dependent NO production is impaired. Liver is one of the organs with the highest XOR concentration. This study was designed to determine whether NO_2^--derived NO by XOR protects liver against I/R injury in vivo. For its minute amounts and active reactivity, NO can not be detected directly in real time in vivo by this time. We have to prove the above hypothesis indirectly. METHODS:Wistar rats were pretreated with saline, NOS inhibitor L-NAME (10 mg/kg intravenously), XOR inhibitor allopurinol (1.5 mg/kg orally), L-NAME +allopurinol and NO scavenger carboxy-PTIO (0.6 mg/kg intravenously) respectively (12 animals per group). And then, they were subjected to total liver ischemia for 40 minutes followed by reperfusion. Blood samples and liver tissues were obtained for analysis after 3 hours of reperfusion. Survival was also investigated. RESULTS:Allopurinol-treated animals exhibited further increased serum alanine aminotransferase (ALT) levels and liver myeloperoxidase (MPO) activities, but further decreased liver adenosine triphosphate (ATP) stores after I/R compared to saline-treated counterparts (830.5±108.3 U/L, 56.5±11.0 U/mg protein and 1.93±0.47 μmol/g vs. 505.8± 184.2 U/L, 41.5±10.2 U/mg protein and 3.05±0.55 μmol/g respectively, P<0.01, P<0.05 and P<0.01 respectively). The hepatocyte injury was further exacerbated and the overall survival rate was significantly decreased after I/R in animals given by allopurinol compared to those pretreated by saline (P<0.05). L-NAME and allopurinol co-treated animals exhibited more severe liver injury (P<0.05 and P<0.01) and a further decreased overall survival rate (P<0.05) compared to L-NAME or allopurinol alone-trea

关键词： nitrites nitric oxide ischemia-reperfusion injury

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Antioxidative effect of propofol during cardiopulmonary bypass in adults

引用

Acta Pharmacologica Sinica 2004年第3期25卷 80-86页

作者： Shi-hai ZHANG2,Shou-yong WANG,Shang-long YAO department of Anesthesiology,union hospital,tongji medicalcollege,huazhong universityof science & technology,wuhan 430022.china department of Anesthesiology Union Hospital Tongji Medical College Huazhong University of Science & Technology Wuhan 430022 China

AIM: To investigate the antioxidative potential of propofol (an intravenous anesthetic with a chemical structure similarto phenol-basedfree radicalscavengers suchas vitaminE) during cardiopulmonary bypass(CPB). METHODS: Thirty adult patients referred for elective cardiac procedure with CPB were included and randomly allocated to a propofol group or a control group. Patients in the propofol group received propofol (0.1 mg·kg-1·min-1) intrave- nously for anesthesia maintenance, whereas those allocated to the control group received fentanyl 10 μg/kg intrave- nously and inhaled enflurane (1 %-1.5 %). Blood samples were collected at 7 time points: before the start of CPB, at 30 and 60 min of CPB, at the conclusion of CPB, 10 min after the administration of protamine, and 12 and 24 h after the cessation of CPB. Plasma levels of free F2-isoprostanes (sensitive markers of free radicals production) and complement C5a were determined by mass-spectrometricassay and enzyme immunoassay, respectively. Neu- trophil adhesion to endothelial cells was observed at ×200 magnificationunder a light microscope. RESULTS: Levels of F2-isoprostanes, complement C5a and neutrophil adhesion rate increased significantly during and after CPB in both groups. There were significantly higher levels of F2-isoprostanes, C5a, and more neutrophils adhering to endothelial cells in the control group than those in the propofol group, respectively. CONCLUSION: Cardio- pulmonary bypass is associated with a great production of damaging free radicals. Propofol may be beneficial both as an anesthetic and as a potent free radical scavenger in patients presenting pathologies associated with free radical reactions during CPB.

关键词： propofol cardiopulmonary bypass free radicals F2-isoprostanes complement 5a

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Local Inhibition of Chlorhexidinum on Lewis Pulm onaryCarcinom a in Mice

引用

Journal of huazhong university of science and technology(medical sciences) 1999年第3期19卷 223-225页

作者： XIONG Jiongxin , HUANG Tao QIU Dengbo department of general surgery, Xiehe hospital, tongji medical university, wuhan 430022 department of general surgery Xiehe Hospital Tongji Medical University Wuhan 430022

C 57 inbred mice ( n =100) were employed to develop animal models of Lewis pulmonary carcinoma. The study on the tumor inhibition was performed by infiltrative injection of 3.5 % Cy or chlorhexidinum of two different concentrations around the tumor respectively. The survival, survival rate, tumor growth rate, and pulmonary metastasis node number were compared. The results showed that the inhibitory effects of 0.1 % and 0.5 % chlorhexidinum were the same as that of 3.5 % Cy, but the toxic and side effects were obviously reduced as compared with 3.5 % Cy. The optimal concentration of chlorhexidinum was 0.5 %. This provides a new approach for infiltrative injection of the tumor for clinical use.

关键词： chlorhexidinum Lewis pulmonary carcinoma

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Intercellular Adhension Molecule-1 in the Pathogenesis of Heroin-induced Acute Lung Injury in Rats

引用

Journal of huazhong university of science and technology(medical sciences) 2004年第5期24卷 430-432页

作者：周琼白明邹世清 department of Respiratory Diseases Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China department of Withdrawal Liyuan Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430072 China

The expression of intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of heroin-induced acute lung injury (ALI) in rats was investigated. The model of ALI was established by intravenous injection of heroin into tail vein in rats. Thirty-six rats were randomly divided into heroin-treated groups (1 h, 2 h, 4 h, 6 h and 24 h) and normal control group. Changes in histopathologic morphology and biological markers of ALI were measured. The expression of ICAM-1 in lung tissue was detected by using immunohistochemistry and RT-PCR. The results showed that the W/D ratio and protein contents in BALF of the heroin-treated groups were significantly higher than that of the control group (P<0.01). The histopathological changes in the lung tissue were more obvious in heroin-treated groups. The ICAM-1 protein and mRNA expression in the lung tissue of heroin-treated groups were significantly increased as compared with that of the control group (P<0.01), and correlated with the ALI parameters in a time-dependent manner. Increasing of ICAM-1 expression was involved in the formation of heroin-induced lung injury. Furthermore, the level of expression was positively correlated with the severity of lung injury.

关键词： heroin acute lung injury intercellular adhesion molecule-1

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Expression of CC Chemokine Ligand 20 and CC Chemokine Receptor 6 mRNA in Patients with Psoriasis Vulgaris

引用

Journal of huazhong university of science and technology(medical sciences) 2004年第3期24卷 297-299页

作者：吴艳李家文 department of Dermatology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China

Summary: In order to explore the possible role of CC chemokine ligand 20 (CCL20) and its receptor CC chemokine receptor 6 (CCR6) in the pathogenesis of psoriasis, the expression levels of mRNA of them in psoriatic lesions were investigated. The skin biopsies were collected from skin lesions in 35 cases of psoriasis vulgaris and 18 normal controls. RT-PCR was used to semi-quantitatively analyze the mRNA expression of CCL20 and CCR6 in the psoriatic lesions and the normal skin tissues. The results showed that the mRNA of CCL20 and CCR6 was present in every specimen. The expression levels of CCL20 mRNA in skin lesions were 1.1397±0.0521, which were greatly higher than those in normal controls (0.8681±0.0308) (P3±0.0538, significantly higher than in the controls (0.9131±0.0433, P<0.001). These findings indicate that up-regulated expression of CCL20 and CCR6 mRNA might be related to the pathogenesis of psoriasis.

关键词： psoriasis CC chemokine ligand 20 CC chemokine receptor 6

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Relationship of CyclinD1 and Estrogen Receptor Expression in the Process of Proliferation and Metastasis in Breast Neoplasm

引用

Journal of huazhong university of science and technology(medical sciences) 2001年第3期21卷 231-232页

作者：王欣邹声泉 department of surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030

The role of CyclinD1 and estrogen receptor (ER) in the process of proliferation and metastasis of breast neoplasm and their relationship were studied. The expression levels of CyclinD1 and ER in the tissue samples were detected by using flow cytometry and L SAB immunohistochemistry staining, respectively. The results showed that CyclinD1 and ER expression levels in breast cancer were significantly higher than in benign breast neoplasm (P0.05), but the CyclinD1 expression level in ER (+) group was significantly higher than in ER (-) group (P<0.05). It was concluded that CyclinD1 expression level might be obviously related with the proliferation and metastasis of breast neoplasm and ER.

关键词： breast cancer CyclinD1 flow cytometry estrogen receptor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Radioiodine labeled SP-4 as an imaging agent foratherosclerotic plaques

引用

Nuclear science and Techniques 2000年第4期11卷 234-239页

作者： ZHANG Yong-Xue, WU Zhi-Jian, CAO Wei (union hospital, huazhong university of science and technology, wuhan 430022) 华中科技大学湖北武汉 430022

The clinical prospect of radioiodinated SP-4 as an atherosclerotic plaque imaging agent was studied. The SP-4 was synthesized by a solid phase method and identified by an amino acid analysis after purification with HPLC. SP-4 was labeled with 131I and 125I by the Chloramine--T method and purified through Sephadex G25 column. Tewlve New Zealand rabbits were divided into an atherosclerotic group (n = 7, AR) and a control group (n = 5, NR ). All of the atherosclerotic rabbits were intravenous administrated with bovine serum albumin, then fed with high cholesterol and fat diet. 125I-SP-4 was intravenous administrated to the rabbits of both groups. The biodistribution of 125I-SP-4 in rabbits was investigated. The uptakes (%ID/g) in blood and thoracic aorta and abdominal aorta were calculated 4 hours postinjection. Macroautoradiography and microautoradiography were performed in 2 AR atheroscle-rotic abdominal aortas. The clearance of radioactivity from plasma was very rapid. 125I-SP-4 was mainly excreted through kidneys. The radioactive uptakes of abdominal aorta and thoracic aorta of AR at 4hours postinjection were significantly higher than that of NR. The films of macroautoradiography showed focal accumulation of the radioactivity in the areas of a newly formed edges of atherosclerotic plaques. On the slices of microautoradiography, the obvious radioactive accumulation could be found in the atherosclerotic plaques. Thus it was seen that the SP-4 remained its biological activity after radioiodination and was located at atherosclerotic lesions, it is potentially useful as an atherosclerotic plaque imaging agent.

关键词：放射性碘标记 SP-4 临床应用放射性显像药物诊断动脉粥样硬化实验兔动物实验

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Expression of the Apoptosis-Suppressive Gene bcl-2 in the Tissue of Colon Carcinoma

引用

Journal of huazhong university of science and technology(medical sciences) 2002年第3期22卷 218-220页

作者：黄文广姚璇 departmentofgeneralsurgery XieheHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430022 departmentofgeneralsurgery XieheHospitalTongjiMedicalCollegeHuazhongUniversityofSci

The role of bcl 2 in the pathogenesis of colorectal tumor were studied. The exp ression of bcl 2 in the colorectal carcinoma and incisional edge tissue of tumo r was detected by using SABC method. The results showed that the positive rate o f bcl 2 was 69.6 % in colorectal carcinoma and 47.6 % in the incisional edge ti ssue respectively, with the difference being very significant ( P =0.001). B cl 2 positive rate was associated with Dukes' stage, but had nothing to do with histological classification. It was concluded that bcl 2 might play a signific ant role in the development of colorectal carcinoma.

关键词： colorectal carcinoma bcl 2 oncoprotein apop tosis immunohistochemistry

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

TGF-beta 1 Gene-Activated Matrices Regulated the Osteogenic Differentiation of BMSCs

引用

Journal of wuhan university of technology(Materials science) 2007年第3期22卷 431-435页

作者：潘海涛郑启新 department of Orthopedics Union HospitalTongji Medical CollegeHuazhong University of Science and Technology

Poly （lactic acid/glycolic acid/asparagic acid-co-polyethylene glycol）（PLGA-[ASP-PEG]） scaffold materials were linked with a novel nonviral vector （K）16GRGDSPC through cross linker Sulfo- LC-SPDP to construct a new type of nonviral gene transfer system. Eukaryotic expressing vector containing transforming growth factor beta 1 （pcDNA3-TGFβ1） was encapsulated by the system. Bone marrow stromal cells （BMSCs） obtained from rabbit were cultured on PLGA-[ASP-PEG] modified by （K）16GRGDSPC and TGF-β1 gene and PLGA-[ASP-PEG] modified by （K）16GRGDSPC and empty vector pcDNA3 as control. The expressions of osteogenic makers of the BMSCs cultured on the TGF-β1 gene-activated scaffold materials were found significantly higher than those of the control group （P〈0.05）. A brand-new way was provided for regulating seed cells to directionally differentiate into osteoblasts for bone defect restoration in bone tissue engineering.

关键词： RGD-containing peptide biomimetic matrix scaffold material osteogenic differentiation bone tissue engineering

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Anti-Proliferative Effect of Inhibitor of Telomerase on Cultured Retinal Pigment Epithelial Cells

引用

Journal of huazhong university of science and technology(medical sciences) 2001年第2期21卷 174-176页

作者：项奕曾水清 department of Ophthalmology Xiehe Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022

In order to provide a new method for treating proliferative vitreoretinopathy , the effects of anti proliferation and apoptosis induction of inhibitors of telomerase and heat shock protein 90 on the cultured retinal pigment epithelial cells were investigated. The rate of apoptosis cells was measured by using TUNEL on the cultured RPE cells, the co cultured RPE cells with inhibitor of telomerase or the co cultured RPE cells with inhibitor of Hsp90 . The cell proliferation status was measured in the above three groups by using MTT method. The rate of apoptosis in the RPE cells co cultured with camptothecin or geldanamycin was increased remarkably . MTT showed the rate of growth inhibition was 8.4 %, 32.3 % and 72.3 % at the concentrations of camptothecin 1 μmol/L, 5 μmol/L, 10 μmol/L, respectively, and 6.5 %, 30.9 %, 71.9 % at the concentrations of geldanamycin 1 μmol/L, 5 μmol/L, 10 μmol/L, respectively. It was concluded that telomerase and Hsp90 can promote the proliferation of the cultured RPE cells, while the inhibitor of them can induce apoptosis and inhibit the growth of the RPE cells.

关键词： camptothelin geldanamycin anti proliferative apoptosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：