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Controlling N-methyl-D-aspartate receptor subunit 1 with calcitonin gene related peptide after cerebral ischemic injury

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Neural Regeneration Research 2006年第7期1卷 585-588页

作者： Jixiang Cui1, Peng Qu2, Chunping Qiao3 1department of Clinical Laboratory, Fourth Affiliated hospital of china Medical University, Shenyang 110032, Liaoning province, china 2department of Neurobiology, Basic Medical College of china Medical University, Shenyang 110032, Liaoning province, china 3department of emergency, baicheng central hospital of jilin province, baicheng 137000, jilin province, china department of Clinical Laboratory Hospital of China Medical University Shenyang 110032 Liaoning Province China department of Neurobiology Basic Medical College China Medical University Shenyang 110032 Liaoning Province China department of emergency Baicheng Central Hospital of Jilin Province Baicheng 137000 Jilin Province China

BACKGROUND: Activation of N-methyl-D-aspartate receptor (NMDAR) is a key link of exitotoxicity at the phase of cerebral ischemic injury. Because NMDAR is a main way to mediate internal flow of Ca2+ among glutamic acid receptors, over-excitation can cause neuronal apoptosis. Calcitonin gene related peptide has a strongly biological activity. On one hand, it can protect ischemic neurons through inhibiting the expression of NMDAR1 mRNA; on the other hand, it can play the protective effect through down-regulating the expression of NMDAR1 mRNA by exogenous calcitonin gene related peptide. OBJECTIVE: To observe the expression of NMDAR1 and the regulatory effect of calcitonin gene related peptide on the expression of NMDAR1 mRNA and protein in the cerebral cortex of rats with focal cerebral ischemia/reperfusion (I/R). DESIGN: Randomized controlled animal study. SETTING: china Medical University. MATERIALS: A total of 216 healthy male Wistar rats, general grade, weighing 250-280 g, were selected in this study. Twelve rats were randomly selected to regard as control group; meanwhile, other 204 rats were used to establish middle cerebral artery occlusion/reperfusion (MACO) models. The main reagents were detailed as follows: calcitonin gene related peptide (Sigma Company); calcitonin gene related peptide kit (Boster Company); antibody Ⅰ, Ⅱ and antibody β-actin Ⅰ, Ⅱ of NMDAR1 mRNA and chemiluminescence reagent (Santa Cruz Company, USA). METHODS: The experiment was carried out in the Laboratory of Neurobiology of china Medical University from August 2005 to June 2006. ① Right MCAO models of rats were established to cause focal ischemia and scored based on Zea Longa five-grade scale. If the scores were 1, 2 and 3 after wakefulness, the MACO models were established successfully and involved in the experiment. A total of 120 rats with successful modeling were randomly divided into I/R group and administration group with 60 in each group. All rats in the both groups were observed at

关键词： NMDAR mRNA Controlling N-methyl-D-aspartate receptor subunit 1 with calcitonin gene related peptide after cerebral ischemic injury gene

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Molecular Targets of Combined Natural Antioxidants Against Diabetes in Streptozotocin-induced Diabetic Mice

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Chemical Research in Chinese Universities 2012年第1期28卷 96-102页

作者： SONG Yang1,2, GAO Zhong-li2, DU Zhen-wu1, PIAO Song-lan1, WANG Ling-ling1, WANG Qing3 and ZHANG Gui-zhen1 1. central Research department, 2. department of Orthopaedics, 3. department of Endocrinology, china-Japan Union hospital of jilin University, Changchun 130033, P. R. china jilin Univ China Japan Union Hosp Dept Endocrinol Changchun 130033 Peoples R China jilin Univ China Japan Union Hosp Cent Res Dept Changchun 130033 Peoples R China jilin Univ China Japan Union Hosp Dept Orthopaed Changchun 130033 Peoples R China

Natural antioxidants, due to the different chemical structures and their unique biological protection effects on islet cell, have been received much attention in applied chemistry field for developing a new diabetic drug. However, the molecular targets of the islet cell protection have been remained obscure. For investigating molecular targets of combined natural antioxidant’s（CNA） roles in against diabetes, type 1 diabetic mouse model was made by multiple injection of low doses of streptozotocin into C57BL mice. Two combinations of natural antioxidants were orally supplemented to the mice. Blood glucose level, glutathione peroxidase（GSH-Px） and superoxide dismutase （SOD） activities, and malondialdehyde（MDA） contents were measured via biochemical assays. Tumor necrosis factor α（TNF-α）, interferon γ（IFN-γ） and Interleukin-12（IL-12） in CD4＋ Peripheral blood mononuclear cells（PBMC） were evaluated with flow cytometry（FCM）. In situ hybridization（ISH） and immunohistochemistry（IHC） were used to detect insulin gene expression. The results demonstrate both the combinations significantly： increased GSH-px and SOD, but decreased MDA levels in pancreas（p〈0.05―0.01）; up-regulated insulin expression at both mRNA and protein levels in islet cells（p〈0.05―0.01）; down-regulated TNF-α and IL-12 expression in PBMC（p〈0.05―0.01）. Our data suggest that the CAN may regulate multiple key molecular targets related to β cell function, including reduction of oxidative stress and pre-inflammatory cytokines, and upregulate insulin gene expression to reduce the development of diabetes.

关键词： Combined natural antioxidant Diabetes Insulin gene expression Oxidative stress Pro-inflammatory cytokine

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Management of traumatic hemothorax by closed thoracic drainage using a central venous catheter

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第1期13卷 43-48页

作者： Jian-hua YI1,Hua-bo LIU2,Mao ZHANG1,Jun-song WU1,Jian-xin YANG1,Jin-ming CHEN1,Shan-xiang XU1,Jian-an WANG1(1department of emergency,the Second Affiliated hospital,School of Medicine,Zhejiang University,Hangzhou 310009,china)(2department of emergency,Zhoushan hospital of Zhejiang province,Zhoushan 316004,china) department of emergency the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China.

Objective:To evaluate the efficacy and safety of the treatment of traumatic hemothorax by closed pleural drainage using a central venous catheter(CVC),compared with using a conventional chest ***:A prospective controlled study with the Ethics Committee approval was undertaken.A total of 407 patients with traumatic hemothorax were involved and they were randomly assigned to undergo closed pleural drainage with CVCs(n=214) or conventional chest tubes(n=193).The Seldinger technique was used for drainage by CVC,and the conventional technique for drainage by chest *** the residual volume of the hemothorax was less than 200 ml after the daily volume of drainage decreased to below 100 ml for two consecutive days,the treatment was considered *** correlative data of efficacy and safety between the two groups were analyzed using t or chi-squared tests with SPSS 13.0.A P value of less than 0.05 was taken as indicating statistical ***:Compared with the chest tube group,the operation time,fraction of analgesic treatment,time of surgical wound healing,and infection rate of surgical wounds were significantly decreased(P0.05),or in the mean catheter/tube indwelling time and mean medical costs of patients treated successfully(P>0.05).Conclusions:Management of medium or large traumatic hemothoraxes by closed thoracic drainage using CVC is minimally invasive and as effective as using a conventional large-bore chest *** complications can be prevented and it has the potential to replace the large-bore chest tube.

关键词： central venous catheter Drainage Trauma Hemothorax

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Carboxyfluorescein Diacetate Succinimidyl Ester Fluorescent Dye for Cell Labeling

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Acta Biochimica et Biophysica Sinica 2005年第6期37卷 379-385页

作者： Xiao-Qi WANG Xiu-Mei DUAN Li-Hua LIU Yan-Qiu FANG Yan TAN The central Laboratory. the First Teaching hospital of jilin University. Changchun 130021, china The central Laboratory the First Teaching Hospital of Jilin UniversityChangchun 130021China

Our objective was to study the properties of the carboxyfluorescein diacetate succinimidylester(CFDA-SE)and the methodology of cell labeling using CFDA-SE fluorescent ***,we analyzedthe kinetics of CFDA-SE fluorescent dye intensity over ***,we determined the optimal concentrationof CFDA-SE fluorescent dye for cell ***,we tested the toxicity of CFDA-SE fluorescent dye onlabeled ***,we determined the optimal staining time of CFDA-SE fluorescent dye for cell *** results show that the optimal concentration of CFDA-SE fluorescent dye for cell labeling varies accordingto different cell ***-SE fluorescent dye is non-toxic to cells as the cell death rate caused by CFDA-SE labeling is below 5%.The optimal cell labeling time was determined to be 8 min of incubation withCFDA-SE fluorescent *** concluded that the advantages of using CFDA-SE fluorescent dye for celllabeling are as follows:(1)the binding of CFDA-SE fluorescent dye to cells is stable;(2) CFDA-SE fluorescentdye is not toxic and does not modify the viability of labeled cells;and(3)CFDA-SE fluorescent dye is asuitable fluorochrome for cell labeling.

关键词： carboxyfluorescein diacetate succinimidyl ester(CFDA-SE) carboxyfluorescein succinimidyl ester(CFSE) flow cytometry

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髂腰肌囊扩张的MRI诊断

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实用放射学杂志 2008年第11期24卷 1524-1527页

作者：班秀丽李娇妍提正太吉林省松原市中心医院放射科

目的探讨髂腰肌囊扩张的MRI表现特征。方法回顾性分析经B超导引下穿刺抽吸和/或经手术病理或临床随访证实的具有典型MRI表现的13例髂腰肌囊扩张病人的MRI表现。结果13例扩张髂腰肌囊均发生于单侧,右侧5例,左侧8例,出现于全部或部分髋臼... 详细信息

目的探讨髂腰肌囊扩张的MRI表现特征。方法回顾性分析经B超导引下穿刺抽吸和/或经手术病理或临床随访证实的具有典型MRI表现的13例髂腰肌囊扩张病人的MRI表现。结果13例扩张髂腰肌囊均发生于单侧,右侧5例,左侧8例,出现于全部或部分髋臼层面上,上下范围最大径线约为7.0cm。为圆形、卵圆形、倒水滴状或不规则分房状囊性肿物,位于髋关节囊前方,髂外或股动静脉外后方和髂腰肌(腱)内侧。MRT1WI呈低、等信号,T2WI呈水样高信号,T2WI+脂肪抑制呈明显高信号,平扫囊壁难以辨认。5例做增强扫描,3例囊壁呈线样均匀轻-中度强化,1例囊壁未见强化,1例呈厚薄不均不规则明显分房状强化,为单纯性化脓性滑膜炎病人。13例中,2例为单纯性滑膜炎症,11例伴髋关节病变。结论MRI对髂腰肌囊扩张的诊断具有重要意义。

关键词：髂腰肌髋关节滑膜囊肿体层摄影术,X线计算机磁共振成像

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急诊科非医疗纠纷的原因分析及对策

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护理研究 2004年第4期18卷 365-366页

作者：付沫湖北省荆州市中心医院

非医疗纠纷是指在医院发生的由医疗以外的原因引起的医患双方的纠纷[1].与医疗纠纷的区别在于医患双方争议的焦点不是诊疗护理引起的不良后果,而是由于其他非诊疗护理因素导致的纠纷.

关键词：医疗纠纷医患沟通民事纠纷急诊病人医务人员医患双方原因分析

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急性冠状动脉综合征sCD40L水平的临床意义

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吉林医学 2005年第10期26卷 1018-1019页

作者：尚士芹刘斌张基昌安喆吉林市中心医院吉林吉林132011 吉林大学第二医院吉林长春130041

目的:探讨急性冠状动脉综合征(ACS)患者血清可溶性CD40L(sCD40L)水平变化的临床意义。方法:应用酶联免疫法(Enzyme-linkedImmunosorbentAssay,EIA)对入选的60例ACS患者(不稳定型心绞痛,UA)48例、急性心肌梗死(AMI)12例的外周血sCD40L水... 详细信息

目的:探讨急性冠状动脉综合征(ACS)患者血清可溶性CD40L(sCD40L)水平变化的临床意义。方法:应用酶联免疫法(Enzyme-linkedImmunosorbentAssay,EIA)对入选的60例ACS患者(不稳定型心绞痛,UA)48例、急性心肌梗死(AMI)12例的外周血sCD40L水平进行检测,及60名正常对照者血清sCD40L的浓度进行检测。结果:ACS组血清sCD40L水平明显较对照组高(P<0.01);AMI血清sCD40L明显高于UA组高(P<0.05);随访期ACSsCD40L增高组心血管事件发生率明显高于正常组。结论:sCD40L的升高在ACS发生和发展中起重要作用,是反应斑块不稳定性的指标之一。

关键词： CD40配体急性冠脉综合征动脉粥样硬化

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废用对松质骨结构的影响

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生物医学工程研究 2004年第3期23卷 129-132,148页

作者：张春秋王福荣郭玉香军事医学科学院卫生装备研究所吉林大学白求恩医学院放射科吉林长春130031 吉林省人民医院急诊外科吉林长春130032

正常骨结构形态的变化总是与力学环境相适应的 ,废用状态可引起松质骨结构的较大改变。本研究将有限元分析与带有调整目标的骨再造方程相结合 ,模拟废用对松质骨结构的影响。数值模拟表明 ,当某方向载荷变小时 ,此方向的骨小梁变细 ,结... 详细信息

正常骨结构形态的变化总是与力学环境相适应的 ,废用状态可引起松质骨结构的较大改变。本研究将有限元分析与带有调整目标的骨再造方程相结合 ,模拟废用对松质骨结构的影响。数值模拟表明 ,当某方向载荷变小时 ,此方向的骨小梁变细 ,结构质量下降 ;当某方向载荷急剧变小 (严重废用 ,如长期卧床 )时 ,此方向很多骨小梁消失 ,废用破坏了松质骨结构的完整性 ;当从废用状态恢复到正常活动时 ,消失的骨小梁不能恢复。这种废用状态引起的结构完整性变化是不可逆的。废用引起的松质骨孔隙变大 ,可能是老年人骨质疏松发生的原因之一。

关键词：松质骨结构废用状态有限元数值模拟松质骨孔隙

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风险管理在急诊护理安全工作中的实施及效果

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护理研究 2007年第36期21卷 3367-3368页

作者：李小平山西省太原市中心医院

护理风险管理是指对病人、工作人员、探视者可能产生伤害的潜在风险进行识别、评价并采取正确行动的过程[1].急诊科是高风险科室,急诊护理面临各种创伤,车祸伤、殴打伤以及意外灾害事故所致突发伤,成批伤员的救治,伤情危重凶险,病人在... 详细信息

护理风险管理是指对病人、工作人员、探视者可能产生伤害的潜在风险进行识别、评价并采取正确行动的过程[1].急诊科是高风险科室,急诊护理面临各种创伤,车祸伤、殴打伤以及意外灾害事故所致突发伤,成批伤员的救治,伤情危重凶险,病人在存活与死亡之间的时间宽限度非常狭小,护理工作预见性差,使急救护理风险加大.鉴于此,我院急诊科重新识别和确认护理风险,2006年1月开始将风险管理运用于急诊护理中,建立护理风险管理机制,实施安全管理措施,取得了良好效果.

关键词：急诊护理病人家属病人转运质控员殴打伤差错事故

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ISO9000质量认证标准在急诊留观室病房化管理中的应用

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护理研究 2004年第24期18卷 2234-2235页

作者：罗淑玲山西省太原市中心医院集团总院

医院急诊管理既有一般科室的共性,也有其特殊性,特别是医疗保险制度改革后,急诊就诊与留观医疗保险费支付的比例不同,病人对急诊留观室的需求也在不断变化.按病情需要观察1 d～3 d的急诊病人被安排在临时输液室观察,而患多科合并疾病和... 详细信息

医院急诊管理既有一般科室的共性,也有其特殊性,特别是医疗保险制度改革后,急诊就诊与留观医疗保险费支付的比例不同,病人对急诊留观室的需求也在不断变化.按病情需要观察1 d～3 d的急诊病人被安排在临时输液室观察,而患多科合并疾病和无入院指证或有入院指证暂时无法收入院的重症病人被收治在急诊留观室内.

关键词：急诊留观 ISO9000 病人病房急诊管理护理质量护理服务质量

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