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Association of apolipoprotein E 4 polymorphism with cerebral infarction in chinese Han population

Acta Pharmacologica Sinica 2004年第03期25卷 98-102页

作者： Zhu-qing JIN2,Yong-sheng FAN3,Jing DING4,Mei CHEN5,Wei FAN4,Guang-ji ZHANG2,Bin-hui ZHANG5,Suo-jing YU2,Yong-sheng ZHANG2,Wei-feng JI5,Jian-gang ZHANG6 2Genomic Research Centre,zhejiang college of traditional chinese medicine,hangzhou 310053 3schoolof basic medical sciences,zhejiang college of traditional chinese medicine,hangzhou 310053 4Department of Neurology,Zhongshan Hospital,Fudan University,Shanghai200032 5Department of Neurology,zhejiang Hospital of traditional chinese medicine,zhejiang collegeof traditional chinese medicine,hangzhou 310006,China Genomic Research Centre Zhejiang College of Traditional Chinese Medicine Hangzhou 310053 School of basic medical sciences Zhejiang College of Traditional Chinese Medicine Hangzhou 310053 Department of Neurology Zhongshan Hospital Fudan University

AIM: To study the association between APOE polymorphisms and cerebral infarction through a case-control study among the chinese Han population. METHODS: First-ever cerebral infarction patients (n=226) whose ages ranged from 40 to 60 years old were recruited from Department of Neurology, Zhongshan Hospital, Shanghai, and zhejiang chinese traditional medicine Hospital, zhejiang, China. Unrelated healthy controls (n=201) were selected from the general population in the same area with similar age and sex distribution. APOE was amplified by one-stage PCR using the forward primer: 5'-GGC ACG GCT GTC CAA GGA GCT-3' and reverse primer: 5'-GAT GGC GCTGAG GCC GCG CT-3'. The PCR product was digested directly with 5 U of CfoI and separated by a 20 % polyacryla- mide (acrylamide: bis-acrylamide=29:1) nondenaturing gel. RESULTS: Both cerebral infarction patient and control groups were in Hardy-Weinberg equilibrium. The allele frequency of APOE*2, APOE*3, and APOE*4 was 4.6 %, 81.9 %, and 13.5 % respectively in the patients with cerebral infarction; 5.7 %, 87.3 %, and 7.0 % respectively in the healthy control group. Compared with APOE3/3 subjects, APOE4/4 carriers had a 2.1-fold risk of cerebral infarction (odds ratio 2.1, 95 % confidence limits 1.3 to 3.4). The allele frequency of APOE*4 in the cerebral infarction patient group was significantly higher than that in the control group (13.5 % vs 7.0 %; P=0.002). CONCLUSION: APOE 4 is a risk factor for cerebral infarction among the chinese Han population.

关键词： apolipoproteins E human alleles polymorphism brain infarction

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Influence of BOL on hyaluronic acid,laminin and hyperplasia in hepatofibrotic rats

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World Journal of Gastroenterology 2001年第6期7卷 872-875页

作者： Li Yao~1 Zhen-Min Yao~2 Tao Yu~2 1 Pharmacology Department of zhejiang college of traditional chinese medicine(TCM),hangzhou 310053,zhejiang Province,China2 basic medicine Department of zhejiang college of TCM,hangzhou 310053,zhejiang Province,China Pharmacology Department of zhejiang college of traditional chinese medicine Hangzhou 310053 Zhejiang Province China. ylyj@***

AIM: To study the anti-hepatofibrosis mechanism of Bie Jia Jian oral liquid (BOL). METHODS: The model was induced by subcutaneous injection of CCl(4). BOL was administered and the change of serum hyaluronic acid (HA) and laminin (LN) was observed and the degeneration of liver cells and the degree of fibre hyperplasia analyzed. Changes of ultra micro-structure in liver cells were observed in some samples. RESULTS: HA was reduced in both the groups with low and high dosage of BOL, which showed a remarkable difference as compared with that of the model group (low dosage group: 376.15 microg/L+/-35.48 microg/L vs 806.07 microg/L+/-98.49 microg/L P340.14 microg/L+/-30.18 microg/L vs 806.07 microg/L+/-98.49 microg/L P33.94 microg/L+/-14.45 microg/L P 33.94 microg/L+/-14.45 microg/L P3 microg/L P 3 microg/L P 3.24 P<0.05) and the pathological change of ultra micro-structure as well. CONCLUSION: The BOL has certain therapeutic effect on the experiment hepatofibrosis. Its mechanisms might include: protecting the function of liver cells, inhibiting excessive synthesis and secretion of extracellular matrix from hepatic stellate cells, relieving the capillarization of hepatic sinusoid, improving liver micro-circulation, and regulating immune function.

关键词： medicine, chinese traditional Animals Hyaluronic Acid Hyperplasia Laminin Liver Cirrhosis Rats Rats, Sprague-Dawley

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Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector

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Journal of zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第9期9卷 728-733页

作者： Jin-zhong XU, Jie SHEN, Yi-yu CHENG, Hai-bin QU (Department of chinese medicine Science and Engineering, college of Pharmaceutical sciences, zhejiang University, hangzhou 310058, China) Department of chinese medicine Science and Engineering College of Pharmaceutical Sciences Zhejiang University Hangzhou 310058 China.

A high-performance liquid chromatographic (HPLC) method with ultraviolet (UV) detector had been developed for simultaneous quantification of danshensu, protocatechuic aldehyde, caffeic acid, salvianolic acid D, rosmarinic acid, salvianolic acid B and salvianolic acid A in Danshen injection. According to the UV spectra of these components, three detection wavelengths have been selected as follows: 280 nm for danshensu and protocatechuic aldehyde, 326 nm for caffeic acid, salvianolic acid D and rosmarinic acid, 286 nm for salvianolic acid B and salvianolic acid A. The limit of detection (LOD) was improved to be in the range of 0.008~0.160 μg/ml. Moreover, excellent linear behavior over the investigated concentration range was observed, with R>0.999 for all the analytes.

关键词： High-performance liquid chromatography (HPLC) Danshen injection (DSI) Phenolic acids Multi-wavelengths Quantification

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Diels-Alder Reaction of Hedychenone and Maleic Anhydride

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chinese Chemical Letters 1999年第7期10卷 531-532页

作者： Qing ZHAO Cheng ZOU Xiao Jiang HAO Yao Zu CHEN(Yunnan college of traditional chinese medicine,Kunming 650200)(Faculty of Pharmacy, Kunming medical college. Kunming 650031)(Kunming Institute of Botany, the chinese Academy of sciences-Kunming 650204)(Zhe Yunnan Coll Tradit chinese Med Kunming 650200 Peoples R China Kunming Med Coll Fac Pharm Kunming 650031 Peoples R China chinese Acad Sci Kunming Inst Bot Kunming 650204 Peoples R China zhejiang Univ Hangzhou 310027 Peoples R China

Diels-Alder reaction of hedychenone 1 and maleic anhydride 2 gave several products 4 similar to 7, whose structures were identified by spectral methods. Effect of temperature on the reaction was disscussed.

关键词： Diels-Alder reaction hedychenone antitumor activity hedychium genus

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超声引导微波消融治疗子宫腺肌病临床应用专家共识(2024版)

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中华超声影像学杂志 2024年第9期33卷 737-759页

作者：中华医学会超声医学分会中华医学会妇科肿瘤学分会中国医师协会介入医师分会上海超声诊疗工程技术研究中心复旦大学超声医学与工程研究所同济大学医学院超声医学研究所中国中医药信息学会中西医结合介入分会中国医学装备协会超声装备技术分会上海市医学会超声分会余松远徐辉雄汪辉张会丽李小龙范培丽孙丽萍彭成忠黄秀峰徐栋不详同济大学上海第十人民医院上海200072 复旦大学附属中山医院上海200032 浙江大学医学院附属妇产科医院杭州310003 同济大学附属第十人民医院复旦大学附属中山医院浙江大学医学院附属妇产科医院浙江省肿瘤医院

子宫腺肌病(adenomyosis,AM)在生育年龄妇女中的发病率为20%~30%,其病理改变是子宫内膜腺体和间质侵入子宫肌层生长、周期性出血、纤维组织增生,形成子宫局限性或者弥漫性病变[1,2,3]。AM发病与雌激素依赖有关,真正发病机制仍然未明。A... 详细信息

子宫腺肌病(adenomyosis,AM)在生育年龄妇女中的发病率为20%~30%,其病理改变是子宫内膜腺体和间质侵入子宫肌层生长、周期性出血、纤维组织增生,形成子宫局限性或者弥漫性病变[1,2,3]。AM发病与雌激素依赖有关,真正发病机制仍然未明。AM具有非致命性、难根治性、高复发性、妊娠期和绝经后自限性等特点。临床上主要表现为子宫体积增大和形态改变、月经量增多、痛经和不孕等,可严重影响患者的生活质量和身体健康[4]。目前,临床上AM的主要治疗方式有药物治疗、经腹和经阴道开放手术或腔镜下子宫切除术、子宫腺肌瘤挖除术、子宫动脉栓塞术(uterine arterial embolization,UAE)、消融治疗、辅助生育治疗等[4-8]。

关键词：子宫动脉栓塞术弥漫性病变子宫腺肌瘤子宫肌层子宫腺肌病子宫切除术未明 arterial

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Anticancer effect of two diterpenoid compounds isolated from Annona glabra Linn

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Acta Pharmacologica Sinica 2004年第7期25卷 91-96页

作者： Yong-hong ZHANG2, Hai-yan PENG3, Guo-hao XIA3, Ming-yan WANG3, Ying HAN4 First Clinical college 3basic medicine college, Nanjing University of traditionalchinese medicine, Nanjing 210029 4Jiangsu Institute of Materia Medica, Nanjing 210009, China First Clinical college basic medicine college Nanjing University of Traditional Chinese Medicine Jiangsu Institute of Materia Medica

AIM: To study the inhibitory effect of two diterpenoid compounds isolated from Annona glabra Linn (Cunabic acid and ent-kauran-19-al-17-oic acid) on the proliferation of Human Liver Cancer (HLC) cell line SMMC-7721 and its mechanism. METHODS: Inhibition of cell proliferation was measured by MTT assay. The morphological changes of SMMC-7721 cells were observed under inverted phase-contrast microscope, fluorescent microscope, transmission electron microscope (TEM), and scanning electron microscope (SEM). Flow cytometer (FCM) was used to calculate the cell apoptotic rate, and immunohistochemical staining was used to observe the regulation of gene expression. RESULTS: The proliferation of SMMC-7721 cells was obviously inhibited after being treated with Cunabic acid at the concentration >5 μmol/L and ent-kauran-19-al-17-oic acid >10 μmol/L. The biggest inhibitory effect was 81.05 % when treated with Cunabic acid at the concentration of 25 μmol/L. The effect had a linear relationship with concentration. The result indicated that drug-treated cells exhibit typical morphological changes of apoptosis, including condensed chromatin and a reduction in volume. Sub-G0/G1 peak was found by FCM analysis and the cell cycle was arrested at G0/G1 stage. The apoptotic rates of the cells treated by Cunabic acid and ent-kauran-19-al-17-oic acid were 43.31 % and 24.95 %, respectively. It was visualized by immunohis- tochemical staining that the drugs down-regulated the gene expression of bcl-2 gene and up-regulated that of bax gene. CONCLUSION: The two diterpenoid compounds isolated from Annona glabra Linn, Cunabic acid and ent- kauran-19-al-17-oic acid can obviously inhibit the proliferation of HLC cell line SMMC-7721. The mechanism is correlated with the induction of cell apoptosis by down-regulating the gene expression of bcl-2 gene and up- regulating that of bax gene.

关键词： Annona glabra Linn diterpenes hepatocellular carcinoma apoptosis gene expression

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Pranlukast reduces neutrophil but not macrophage/microglial accumu-lation in brain after focal cerebral ischemia in mice

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Acta Pharmacologica Sinica 2006年第3期27卷 282-288页

作者： Li-sheng CHU~(2,3) Er-qing WEI~(2,4) Guo-liang YU~2 San-hua FANG~2 Yu ZHOU~2 Meng-ling WANG~2 Wei-ping ZHANG~2 ~2Department of Pharmacology,School of medicine zhejiang University.hangzhou 310031.China:~3Department of Physiology.zhejiang college of traditional chinese medicine.hangzhou 310053,China DepartmentofPharmacology SchoolofMedicineZhejiangUniversityHangzhou310031China DepartmentofPhysiology ZhejiangCollegeofTraditionalChineseMedicineHangzhou310053China

Aim: To determine whether pranlukast, a cysteinyl leukotriene receptor-lantagonist, exerts an anti-inflammatory effect on focal cerebral ischemia in ***: Focal cerebral ischemia in mice was induced by permanent middle cere-bral artery occlusion(MCAO). In addition to neurological deficits, infarct volume,degenerated neurons and endogenous IgG exudation, we detected accumulationof neutrophils and macrophage/microglia in the ischemic brain tissue 72 h afterMCAO. Pranlukast was ip injected 30 min before and after MCAO. Results:Pranlukast significantly attenuated neurological deficits, infarct volume, neurondegeneration and IgG exudation. Importantly, pranlukast(0.01 and 0.1 mg/kg)inhibited myeloperoxidase-positive neutrophil, but not CD11b-positive macroph-age/microglial accumulation in the ischemic cortical tissue. Conclusion: Pranlukastexerts an anti-inflammatory effect on focal cerebral ischemia in the subacute phasethat is limited to neutrophil recruitment through the disrupted blood-brain barrier.

关键词： leukotriene receptor antagonist pranlukast cerebral ischemia neuroprotection inflammation

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A peroxisome proliferator response elements regulatory system in xenopus oocytes and its application

A peroxisome proliferator response elements regulatory syste...

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华东六省一市生物化学与分子生物学学会2006年学术交流会

作者： YAN Jin,FAN Chun-lei,WO Xing-de and GAO Li-ping (School of medicine,hangzhou Normal college, hangzhou 310018, China Department of Life Science,zhejiang college of traditional chinese medicine, hangzhou 310053,China)

Background Peroxisome proliferator-activated receptor-gamma (PPARγ) is a kind of ligand -activated transcription factors binding to peroxisome proliferator response element (PPRE), a specific recognition site. It is thought to play a critical role in glucose and lipid metabolism and in inflammation control. The aim of this study was to establish a new cellular model for the quick screening of lipid-lowering drugs, which may be effective as PPAR-γ ligands on the PPRE-mediated pathway regulatory system. Methods Two plasmids were constructed: pXOE-PPARY, in which the human PPAR7 gene was in the downstream of TF Ⅱ A gene promoter, and pLXRN-PPRE-d2EGFP, in which the enhanced green fluorescent protein (EGFP) gene was subcloned into PPRE. The xenopus oocytes were injected with these two plamids, and consequently treated with prostaglandin E1, pioglitazone, and different kinds of lipid-lowering drugs. After 3 days, the oocytes were observed under a fluorescence microscope. To confirm the drug action,we injected pXOE-PPARY plasmid into the oocytes, which then treated with prostaglandin Eland Hawthorn flavonoids. The mass of expressed lipoprotein lipase (LPL) in the cells was determined by enzyme labeling linked immunosorbent assay (ELISA). Results The expression of EGFP was only induced by prostagalandin E1, pioglitazone, Hawthorn flavonoids. A concentration-response relationship was seen between expressed EGFP and Hawthorn flavonoids. The levels of LPL in both Hawthorn flavonoids groups and PPARY ligand prostagalandin El group injected with pXOE-PPARY plamid increased significantly (P<0. 001) compared with controls, and a concentration-response relationship was observed between LPL mass and Hawthorn flavonoids. Conclusions It is possible to establish a PPRE regulatory EGFP reporter system in xenopus oocytes to monitor the activity of PPARY ligand. Hawthorn flavonoids can increase the expression of gene downsteam of PPRE by effect on the PPRE pathway regulatory system.

关键词： peroxisome proliferator-activated receptorγ oocytes flavonoids lipoprotein lipase response elements

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Genetic variability of half-smooth tongue sole Cynoglossus semilaevis populations using microsatellite markers

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Acta Oceanologica Sinica 2011年第3期30卷 76-83页

作者： LIU Yunguo GUO Yinghui LIU Shuaishuai LI Junfeng YE Naihao Inspection ＆ Quarantine Technology Center Shandong Entry-Exit Inspection ＆ Quarantine Bureau Qingdao 266002 China Ocean University of China Qingdao 266003 China college of basic medical sciences Shandong University of Traditional Chinese Medicine Jinan 250355 China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China college of Chemical Engineering Qingdao University of Science ＆ Technology Qingdao 266042 China

Ten highly variable microsatellite loci were performed in order to evaluate the genetic variability of wild and hatchery samples of half-smooth tongue sole Cynoglossu＇s semilaevis. A group of 200 genotypes belonging to four wild samples, Laizhou （LZ）, Weihai （WH）, Qingdao （QD）, Rizhao （RZ） and one hatchery sample, Mingbo （MB）, were screened. All of the： ten microsatellite loci screened in this study showed marked polymorphism. A total of 70 different alleles were observed over all loci. The number of alleles per locus ranged from 4.2 to 1006 the average of expected and observed heterozygosity ranged from 0.67 to 0.82, and from 0：77 to0.87 respectively. A total of 10, 16, 10, 11 and 5 unique alleles each were found in LZ, WH~ QD：RZ and MB populations. The effective number of alleles varied from 2.87 for HSTS_7 to 6.83 for HSTS_h. The number of average genotypes ranged from 6.0 for HSTS a to 15.4 for HSTS_h： As compared with the wild populations, the hatchery population, MB, showed significant genetic changes such as fewer alleles per locus （P〈0.05）, a smaller number of low frequency alleles （P〈0.05）, a small number of unique alleles and a small number of genotypes （P〈0.05）, all indicative of a reduction in genetic diversity.

关键词： Cynoglossus semilaevis microsatellite marker population genetic variability

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The Effects of Warm and Tonify Kidney-Yang Herbs on the Liver Mitochondria Proteome of the Kidney-Yang dificency in the Rats

The Effects of Warm and Tonify Kidney-Yang Herbs on the Live...

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浙江省生物化学与分子生物学学术交流会

作者： WO Xing-De, LU De-Zhao, SHI Meng-Ru, LI Yi, TANG Li-Hua (Department of Life Science, zhejiang college of traditional chinese medicine, hangzhou 310053, china)

Two-dimensional difference gel electrophoresis (2-D DIGE) was used to study the difference of liver mitochondria proteome between the normal rats, the kidney-yang deficiency rats and the kidney-yang d

关键词： Proteome. Shenyangxu. Wenbushenyang Mitochondria. Energy metabolism

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