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Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.)

2010中国作物学会学术年会

作者： Yun Wang~(1,2),Lirui Cheng~1,Yong Sun~1,Wenshen Wang~1,Rongshen Guan~1,Linghua Zhu~1, Zhikang Li~(1,3) (1 institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China 2Shenyang Agricultural University,Shenyang 110161,China 3international rice research institute,dapo box7777,manila,philippines.

Two large sets of reciprocal introgression lines(ILs) derived a japonica variety ’Lemont’ and indica variety ’Teqing’,were analyzed using molecular markers to understand the transmission pattern of donor segments in reciprocal indica/japonica recipients of rice and the effects of genetic background on QTL expression of flag leaf width(LW) and spikelet number per panicle(SNP) under the two diverse environments (Beijing and Hainan).Comparison of the linkage maps from the two ILs and RIL populations from the same cross revealed a high frequency and genomewide distribution of inverted marker orders,suggesting the

关键词： genetic background effect intrasubspecific cryptic genomic variation subspecific differentiation genetic load Oryza sativa L.

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Science China(Life Sciences) 2009年第9期52卷 885-890页

作者： SHI YongFeng1,2,CHEN Jie1,LIU WenQiang1,HUANG QiNa1,2,SHEN Bo2,LEUNG Hei3 & WU JianLi1 1 Chinese National Center for rice Improvement/National key Laboratory for rice Biology,China National rice research institute,Hangzhou 310006,China 2 College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 310016,China 3 international rice research institute,Metro manila dapo box 7777,philippines Chinese National Center for rice Improvement/National key Laboratory for rice Biology China National Rice Research Institute Hangzhou 310006 China College of Life and Environmental Sciences Hangzhou Normal University Hangzhou 310016 China international rice research institute Metro Manila DAPO Box 7777 Manila Philippines

To understand the development of rice leaf blades,we identified a new rolled-leaf mutant,w32,from indica cultivar IR64 through EMS mutagenesis. The mutant showed a stable rolled-leaf phenotype throughout the life cycle. Two F2 populations were developed by crossing w32 to cultivar IR24 and PA64. Genetic analysis showed that the rolled-leaf phenotype was controlled by a single recessive gene. To determine the location of the gene,bulked segregant analysis was carried out using mutant and wild-type DNA pools and 1846 mutant-type F2 individuals derived from the cross w32/PA64 were genotyped to locate the gene on the short arm of chromosome 7. The rolled-leaf gene,tentatively named rl11(t),is likely a new gene as no other rolled-leaf genes have been identified near the region. By developing new SSR and InDel markers,the gene was delimited to a 52 kb region near the end of the short chromosome arm. Further fine mapping and cloning of the gene are currently underway.

关键词： rice (Oryza sativa L.) chemical mutagenesis molecular marker rolled-leaf gene physical map

Dissection of genetic overlap of salt tolerance QTLs at the seedling and tillering stages using backcross introgression lines in rice

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Science China(Life Sciences) 2008年第7期51卷 583-591页

作者： ZANG JinPing 1 ,SUN Yong 1 ,WANG Yun 1 ,YANG Jing 1 ,LI Fang 1 ,ZHOU YongLi 1 ,ZHU LingHua 1 , Reys JESSICA2,Fotokian MOHAMMADHOSEIN 2,XU JianLong 1&LI ZhiKang 1,2 1institute of Crop Sciences/National Key Facility for Crop Gene Resources&Genetic Improvement,Chinese Academy of Agricul- tural Sciences,Beijing 100081,China 2international rice research institute,dapo box 7777,Metro manila,philippines 1. institute of Crop Sciences/National Key Facility for Crop Gene Resources & Genetic Improvement Chinese Academy of Agricultural Sciences Beijing 100081 China 2. international rice research institute DAPO Box 7777 Metro Manila Philippines

QTLs for salt-tolerance(ST)related traits at the seedling and tillering stages were identified using 99 BC2F8 introgression lines(IL)derived from a cross between IR64(indica)as a recurrent parent and Binam(japonica)from Iran as the donor *** QTLs affecting survival days of seedlings(SDS), score of salt toxicity of leaves(SST),shoot K + concentration(SKC)and shoot Na + concentration(SNC) at the seedling stage and 22 QTLs underlying fresh weight of shoots(FW),tiller number per plant(TN) and plant height(PH)at the tillering stage were *** QTLs detected at the tillering stage showed obvious differential expression to salt stress and were classified into three types based on their differential *** I included 11 QTLs which were expressed only under the non-stress *** II included five QTLs expressed in the control and the salt stress conditions,and three of them(QPh5,QPh8 and QTn9)had similar quantity and the same direction of gene effect,suggesting their expression was less influenced by salt *** III included six QTLs which were detectable only under salt stress,suggesting that these QTLs were apparently induced by the *** QTLs affecting trait difference or trait stability of ILs between the stress and non-stress conditions were identified and the Binam alleles at all loci except QPh4,QTn2 and QFw2a decreased trait *** three QTLs less influenced by the stress and 13 QTLs affecting trait stability were considered as ST QTLs which contributed to *** the distribution of QTLs detected at the seedling and tillering stages,most(69%)of them were genetically *** four were the same or adjacent regions on chromosomes 1,2,8 and 11 harboring ST QTLs detected at the two stages,suggesting that partial genetic overlap of ST across the two stages *** is likely,therefore,to develop ST rice variety for both stages by pyramiding of ST QTLs of different stages or selection against the ove

关键词： rice salt tolerance(ST) quantitative trait loci(QTL) genetic overlap

Comparative Analysis of Genetic Diversity and Structure in rice Using ILP and SSR Markers

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rice science 2010年第4期17卷 257-268页

作者： HUANG Ming XIE Fang-min CHEN Li-yun ZHAO Xiang-qian L. JOJEE D. MADONNA rice research institute Hunan Agricultural University Changsha 410128 China Plant Breeding Genetics and Biotechnology Division International Rice Research Institute Metro Manila DAPO Box 7777 Philippines Grain Quality Nutrition and Postharvest Center International Rice Research Institute Metro Manila DAPO Box 7777 Philippines

Genetic diversity of 36 rice entries from the United States Department of Agriculture （USDA） rice collection was assessed using 103 ILP （intron length polymorphism） and 54 SSR （simple sequence repeats） markers. A total of 236 and 332 alleles were detected by the ILP and SSR markers, respectively. On average, the SSR markers produced higher polymorphism information content value and number of alleles than the ILP markers. Whereas the Nei＇s genetic distance measured using the SSR markers was much higher than that measured using the ILP markers. Manters test indicated that there was a statistically significant correlation （r=0.827, P〈0.001 ） between the two marker systems. UPGMA clustering based on the ILP and SSR markers resulted in consensus dendrograms. The cophenetic correlation coefficient （r=0.918, 0.878 and 0.924, P〈0.001 for the ILP, SSR and combined markers, respectively） showed a highly accurate dendrogram represented the genetic distance among these entries. The 36 entries were divided into four groups. Four African Oryza glaberrima accessions were clustered within a distinct group （I）, and the remaining entries were separated into three groups （11, III and IV）. All the entries could be also clustered into two main groups： One was composed of III and IV, considered as indica group, and the other was composed of I （O. glaberrima） and II （japonica-like）. Model-based cluster analysis revealed that the japonica-like group maintained very pure ancestry while the indica group shared mixed ancestry, especially for group III, which had seven admixtures sharing from 19.5% to 30.0% of ancestry with group IV （based on SSR markers）. It is suggested that ILP and SSR markers could be very useful for the genetic study and breeding in rice.

关键词： rice genetic diversity cluster analysis structure simple sequence repeats intron length polymorphism

Speed and surface steepness affect internal tibial loading during running

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Journal of Sport and Health Science 2024年第1期13卷 118-124页

作者： Hannah rice Markus Kurz Patrick Mai Leon Robertz Kevin Bill Timothy R.Derrick Steffen Willwacher Department of Physical Performance Norwegian School of Sport SciencesOslo 0806Norway Sports Tech research Centre Mid Sweden UniversityÖstersund SE-83125Sweden Department of Mechanical and Process Engineering Offenburg University of Applied SciencesOffenburg 77652Germany institute of Biomechanics and Orthopaedics German Sport University CologneCologne 50933Germany Department of Kinesiology Iowa State UniversityAmesIA 50011USA

Background:Internal tibial loading is influenced by modifiable factors with implications for the risk of stress *** encounter varied surface steepness(gradients)when running outdoors and may adapt their speed according to the *** study aimed to quantify tibial bending moments and stress at the anterior and posterior peripheries when running at different speeds on surfaces of different ***:Twenty recreational runners ran on a treadmill at 3 different speeds(2.5 m/s,3.0 m/s,and 3.5 m/s)and gradients(level:0%;uphill:+5%,+10%,and+15%;downhill:-5%,-10%,and-15%).Force and marker data were collected synchronously *** moments were estimated at the distal third centroid of the tibia about the medial-lateral axis by ensuring static equilibrium at each 1%of *** was derived from bending moments at the anterior and posterior peripheries by modeling the tibia as a hollow ***-way repeated-measures analysis of variance were conducted using both functional and discrete statistical ***:There were significant main effects for running speed and gradient on peak bending moments and peak anterior and posterior *** running speeds resulted in greater tibial *** uphill at+10%and+15%resulted in greater tibial loading than level *** downhill at-10%and-15%resulted in reduced tibial loading compared to level *** was no difference between+5%or-5%and level ***:Running at faster speeds and uphill on gradients≥+10%increased internal tibial loading,whereas slower running and downhill running on gradients≥-10%reduced internal *** running speed according to the gradient could be a protective mechanism,providing runners with a strategy to minimize the risk of tibial stress injuries.

关键词： Bending moments Gradient Musculoskeletal modeling Overuse injury Tibial stress Training factors

Tagging Blast Resistance Gene Pi 1 in rice (Oryza sativa) Using Candidate Resistance Genes

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rice Science 2004年第Z1期 251-254页

作者： Menchu BERNADO Entomology and Plant Pathology Division international rice research institute dapo P.O. box 7777.Metro manila philippines

An F3 population derived from C101LAC/CO39 containing 90 lines was analyzed for blast resistance with 48 candidate genes developed from resistance gene analogs （RGA） and suppression subtractive library. Genetic analysis confirmed that blast resistance of the population was controlled by a single gene Pi 1. One of the candidate genes, R10 was identified as associated with the blast resistance gene on the long arm of chromosome 11 and mapped using a DH population derived from Azucena/IR64. A pair of PCR based primers was designed based on the sequence of R10 for marker-aided selection of the blast resistance gene. The recombination frequency between Pi 1 and the marker was estimated as 1.28%. It suggested that strategy of employing candidate genes is useful for gene identification and mapping. A new RFLP marker and the corresponding PCR marker for tagging of Pi 1 were provided.

关键词： rice candidate gene marker-aided selection blast resistance

Evaluation of screening effectiveness on stress tolerance in advanced introgression populations in rice

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Evaluation of screening effectiveness on stress tolerance in...

Al_2O_3-Coated Nano-SiC Particles Reinforced Al_2O_3 Matrix Composites

2010中国作物学会学术年会

作者： Lijun Meng~1,Jianlong Xu~1,Xiuyun Lin~2,Haibo Sun~3,Xiufang Ma~4,Linghua Zhu~1,Zhikang Li~(1,5) (1institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China 2institute of rice,Jilin Academy of Agricultural Sciences,Changchun,136100,China 3institute of Crop,Tianjin Academy of Agricultural Sciences,Tianjin,300112,China 4institute of rice,Liaoning Academy of Agricultural Sciences,Shenyang,110101, China 5international rice research institute,dapo box7777,manila,philippines.

Using BCF advanced backcross populations derived from crosses between Chaoyoul as recurrent parent and 11 diverse entries(eight indica varieties and threejaponica varieties) as donor parents,salt tolerance(ST) at the seedling stage and the stage from tillering to mature,cold tolerance(CT) at the booting stage and heat tolerance(HT) at flowering stage were identified and screened under the stress intensity when the check,Chaoyoul completely died or almost infertility under *** percentage of ST plants was much difference among various populations at seedling stage and the stage from tillering

关键词： rice Backcross introgression Salt tolerance Heat tolerance Cold tolerance Hidden gene

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Journal of Materials Science & Technology 2002年第6期18卷 541-543页

作者： Juxian ZHANG, Jinjun FENG and Longqiao GAOBeijing Vacuum Electronics research institute, PO. box 749 Ext. box 33, Beijing 100016, China Beijing Vacuum Electron. Res. Inst. PO Box 749 Beijing 100016 China

Properties of Al2O3-coated nano-SiC have been compared with those of as-received SiC. The isoelectric point (IEP) of SiC changed from pH3.4 to pH7.3 after coating with the alumina precursor, which is close to that of alumina. Because both surfaces of coated SiC and AI2O3 possess higher positive charge at pH=4.5-5.0, they are uniformly dispersed in the two-phase aqueous suspensions, Then a mixed powder containing nano-SiC dispersed homogeneously into the Al2O3 matrix was achieved from flocculating the two-phase suspension. Finally, Al2O3/SiC nanocomposites were obtained by coating nano-SiC with Al2O3, in which the majority of SiC particles were located within the AI2O3 grains. The observation by transmission electron microscopy (TEM) and the analysis by the X-ray photoelectron spectroscopy (XPS) showed that cracks propagated towards the intragranular SiC rather than along grain boundaries.

关键词： Coated nano-SiC Colloidal processing Nanocomposites Intragranular structure Transgranular fracture

Quantifying the effectiveness of SiO_2/Au light trapping nanoshells for thin film poly-Si solar cells

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Science China(Technological Sciences) 2010年第8期53卷 2228-2231页

作者： BAI YiMing 1 ,WANG Jun 2 ,CHEN NuoFu 1,3 ,YAO JianXi 1 ,YIN ZhiGang 3 ,ZHANG Han 3 , ZHANG XingWang 3 ,HUANG TianMao 3 ,WANG YanShuo 3 &YANG XiaoLi 3 1School of Renewable Energy Engineering,North China Electric Power University,Beijing 102206,China 2 National Engineering research Center for Optoelectronic Devices,institute of Semiconductors, Chinese Academy of Sciences,PO box 912,Beijing 100083,China 3 Key Laboratory of Semiconductor Materials Science,institute of Semiconductors, Chinese Academy of Sciences,PO box 912,Beijing 100083,China 1. School of Renewable Energy Engineering North China Electric Power University Beijing 102206 China2. National Engineering Research Center for Optoelectronic Devices Institute of Semiconductors Chinese Academy of Sciences PO Box 912 Beijing 100083 China3. Key Laboratory of Semiconductor Materials Science Institute of Semiconductors Chinese Academy of Sciences PO Box 912 Beijing 100083 China

In order to enhance light absorption of thin film poly-crystalline silicon(TF poly-Si)solar cells over a broad spectral range, and quantify the effectiveness of nanoshell light trapping structure over the full solar spectrum in theory,the effective photon trapping flux(EPTF)and effective photon trapping efficiency(EPTE)were firstly proposed by considering both the external quantum efficiency of TF poly-Si solar cell and scattering properties of light trapping *** EPTF,EPTE and scattering spectrum exhibit different behaviors depending on the geometric size and density of nanoshells that form the light trapping *** an optimum size and density of SiO2/Au nanoshell light trapping layer,the EPTE could reach up to 40%due to the enhancement of light trapping over a broad spectral range,especially from 500 to 800 nm.

关键词： thin film poly-crystalline silicon surface plasmons SiO2/Au nanoshell