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Con A affinity glycoproteomics of normal human liver tissue

Science China(Life sciences) 2007年第3期50卷 403-411页

作者： SUN QiangLing1, LIU YinKun1, LU WenJing1, CHENG Gang2. ZHOU HaiJun1, ZHOU XinWen3, WEI LiMing3, DAI Zhi1, GUO Kun1 & LU HaoJie3 1 Liver Cancer Institute, Zhongshan Hospital, fudan university, Shanghai 2.0032. China 2.System Biology Lab, Institute of biomedical {2., fudan university, Shanghai 2.04.3, China 3 Research Center for Proteomics, Institute of biomedical {2., fudan university, Shanghai 2.04.3, China 1. Liver Cancer Institute Zhongshan Hospital Fudan University Shanghai 200032 China 2. System Biology Lab Institute of Biomedical Sciences Fudan University Shanghai 200433 China 3. Research Center for Proteomics Institute of Biomedical Sciences Fudan University Shanghai 200433 China

In order to establish the novel high throughput, high efficiency and low cost technological platform for the research of N-glycoproteomics, to resolve the significance of characteristic expression profile of glycoprotein and to find the proteins with biological functional importance, the glycoproteins with high-mannose core and the two antennary types were purified and enriched by the Con A affinity chromatography. Con A affinity protein expression profiles of normal human liver tissue were gener- ated by using SDS-PAGE, two-dimensional electrophoresis (2.DE) followed by fast fluorescence stain- ing based on multiplexed proteomics (MP) technology. 301 visible protein spots on the gel were de- tected and 85 of glycoproteins were further successfully identified via peptide mass fingerprinting (PMF) by a matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF- MS/MS) and annotated to IPI databases. Identified glycoproteins definitely take part in the regulation of cell cycle and metabolic processes. The glycosylation sites were predicted with NetNGlyc 1.0 and NetOGlyc 3.1 software, meanwhile they were classified according to the geneontology methods. The construction of Con A affinity glycoprotein database of normal human liver tissue would contribute to the subsequent research.

关键词： Con A affinity glycoproteomics of normal human liver tissue

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Regulation of activity of nuclear factor-kB and activator protein-1 by nitric oxide, surfactant and glucocorticoids in alveolar macrophages from piglets with acute lung injury

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Acta Pharmacologica Sinica 2003年第12期 142-149页

作者： CAO Lei~(2.3). QIAN Li-Ling~2. ZHU You-Rong~2. GUO Chun-Bao~2. GONG Xiao-Hui~2. SUN Bo~(2.4. ~3Laboratory of Respiratory and Intensive Care, Children’s Hospital of fudan university, Shanghai 2.0032.~3Department of Pediatrics, Hebei Provincial People’s Hospital, Shijiazhuang, China

AIM: To investigate whether acute lung injury (ALl) in ventilated piglets with bacterial infection affects NF-κB and AP-1 expression in alveolar macrophages (AM) and whether nitric oxide (NO), surfactant (Surf), glucocorticoids (GC) affect NF-κB and AP-1 activation in AM in vivo and in vitro. METHODS: The animals were intraperitoneally injected Escherichia coli, which caused ALl. Nuclear extracts of AM were analyzed by electrophoretic mobility shift assay (EMSA) for the nuclear factor-kappa B (NF-κB) and activation protein-1 (AP-1) expression. Detection of IκB-α protein was from cytoplasmic extract by Western blotting. Immunocytochemistry staining was used for intracellular location of p65 subunits of NF-κB. RESULTS: In ex vivo experiments, strikingly higher expression of NF-κB and AP-1 by EMSA was found 6 h after bacterial injection in contrast to the Normal group. In the NO, SNO, and GC groups, markedly attenuated NF-κB and AP-1 activation was observed. The NF-κB and AP-1 activation in Surf g

关键词： acute lung injury alveolar macrophages NF-kappa B activator protein-1 nitric oxide surfaceactive agents glucocorticoids

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Electrophysiological actions of cyclosporin A and tacrolimus on rat hippocampal CA1 pyramidal neurons

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Acta Pharmacologica Sinica 2007年第12期28卷 1891-1897页

作者： Yong YU~2.Xue-qin CHEN~3,Yao-yuan CUI~(2.4.,Guo-yuan HU~(3,4.~2.Department of Neurosurgery,Zhongshan Hospital,fudan {4.,Shanghai 2.0032.China ~3 State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of {2.,Shanghai 2.12.3,China Department of Neurosurgery Zhongshan HospitalFudan UniversityShanghai 200032China State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

Aim:The aim of the present study was to investigate the electrophysiologicalactions of cyclosporin A （CsA） and tacrolimus （FK506） on neurons in the brain,and to elucidate the relevant ***:Whole-cell current-clamprecording was made in CA1 pyramidal neurons in rat hippocampal slices;whole-cell voltage-clamp recording was made in dissociated hippocampal CA1 pyrami-dal neurons of ***:CsA （100 μmol/L） and FK506 （50 μmol/L） did notsignificantly alter the passive electrical properties of hippocampal CA 1 pyramidalneurons,but slowed down the repolarizing phase of the action ***（10-100 μmol/L） selectively inhibited the delayed rectifier K+current （IK） in aconcentration-dependent *** did not affect the kinetic properties of *** dialysis of CsA （100 μmol/L） had no effect on *** inhibition of IKby CsA （100 μmol/L） persisted under the low Ca2.conditions that blocked thebasal activity of ***:CsA exerted calcineurin-independentinhibition on the IKin rat hippocampal pyramidal *** together withour previous finding with FK506,it is conceivable that the spike broadening causedby the immunosuppressant drugs is due to direct inhibition on the IK.

关键词： action potential calcineurin cyclosporin A delayed rectifier K~+ channel hippocampus tacrolimus

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The rate of convergence of the Lax-Oleinik semigroup-degenerate fixed point case

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Science China Mathematics 2011年第3期54卷 545-554页

作者： WANG KaiZhi1,2.& YAN Jun1 1School of Mathematical sciences and Key Lab of Mathematics for Nonlinear Science, fudan university, Shanghai 2.04.3, China 2.chool of Mathematics, Jilin {2., Changchun 130012. China 1. School of Mathematical sciences and Key Lab of Mathematics for Nonlinear Science Fudan University Shanghai 200433 China2. School of Mathematics Jilin University Changchun 130012 China

For the standard Lagrangian in classical mechanics, which is defined as the kinetic energy of the system minus its potential energy, we study the rate of convergence of the corresponding Lax-Oleinik semigroup. Under the assumption that the unique global minimum point of the Lagrangian is a degenerate fixed point, we provide an upper bound estimate of the rate of convergence of the semigroup.

关键词： Hamiltonian system weak KAM theorem Lax-Oleinik semigroup

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Association between VDR Apal Polymorphism and Hip Bone Mineral Density Can Be Modified by Body Mass Index:A Study on Postmenopausal Chinese Women

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Acta Biochimica et Biophysica Sinica 2005年第1期 61-67页

作者： Hong XUI~(1,2. Dong-Hai XIONG~3 Fu-Hua XU~3 Yuan-Yuan ZHANG~3 Shu-Feng LEI~1 Hong-Wen DENG~(1,3*) ~1Laboratory of Molecular and Statistical Genetics,College of Life sciences,Hunan Normal university.Changsha 4.0081,China ~2.Department of Physiology,Jiangxi Medical College,Nanchang 330006,China ~3 Osteoporosis Research Center and Department of {2. sciences,Creighton university Medical Center,Omaha,NE 68131,USA

Osteoporosis is a major public health problem for old *** factors are considered tobe major contributors to the pathogenesis of postmenopausal *** vitamin D receptor(VDR)gene is a prominent candidate gene for the regulation of postmenopausal bone mass;however,despite exten-sive studies,controversy remains regarding its association with postmenopausal body mineral density(BMD)*** this study,a total of 2.0 healthy postmenopausal Chinese women were genotyped at the VDRApaI locus using polymerase chain reaction(PCR)-restriction fragment length polymorphism (RFLP).Rawhip BMD was significantly associated with VDR ApaI polymorphism with and without adjusting for age(P=0.015 and 0.04.,respectively).This genetic effect can explain 3.32. of hip BMD ***,the significant association vanished after correcting for both age and body mass index(BMI)(P=0.169).Inaddition,we observed a significant association between VDR ApaI polymorphism with unadjusted BMI(P=0.04.0}or BMI adjusted for age(P=0.04.).The raw hip BMD was also found to be significantly corre-lated with BMI(r=0.517,P=0.0001),with BMI explaining 2..35% of the variation of hip *** of thesefacts prompt us to conclude that the significant association between the VDR ApaI genotype and hip BMDmay be modified by BMI in postmenopausal Chinese *** findings may partially explain the earlierinconsistent association results concerning the VDR gene and BMD,and highlight the importance of incorpo-rating covariates such as BMI into osteoporosis association studies.

关键词： body mass index(BMI) bone mineral density(BMD) genetic association osteoporosis vitamin D receptor(VDR)gene

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Global Existence of Classical Solutions for Some Oldroyd-B Model via the Incompressible Limit

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Chinese Annals of Mathematics,Series B 2006年第5期27卷 565-580页

作者： Zhen LEI School of Mathematical sciences, fudan university, Shanghai 2.04.3, China School of Mathematics and Statistics, Northeast Normal {2., Changchun 13002., China. School of Mathematical sciences Fudan University Shanghai 200433 China School of Mathematics Statistics Northeast Normal University Changchun 130024 China

In this paper, we prove local and global existence of classical solutions for a system of equations concerning an incompressible viscoelastic fluid of Oldroyd-B type via the incompressible limit when the initial data ... 详细信息

关键词： Incompressible limit Global existence Oldroyd model

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Expression,Characterization and Immunogenicity of a Major Outer Membrane Protein from Vibrio alginolyticus

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Acta Biochimica et Biophysica Sinica 2007年第3期39卷 194-200页

作者： Ronghua QIAN~(1,2. Wuying CHU~3 Zhijuan MAO~(1,4. Chongwen ZHANG~(1,2. Yongwei WEI~2.Lian YU~2. 1 Department of {4. Engineering,Zhejiang university,Hangzhou 31002.,China 2.Institute of Preventive Veterinary Medicine,Zhejiang university,Hangzhou 31002.,China 3 Institute of Subtropical Agriculture,Chinese Academy of sciences,Changsha 4.012.,China 4.Ningbo Oceanography and Fishery Institute,Ningbo 315012.China DepartmentofbiomedicalEngineering ZhejiangUniversityHangzhou310027China InstituteofPreventiveVeterinaryMedicine ZhejiangUniversityHangzhou310029China InstituteofSubtropicalAgriculture ChineseAcademyofSciencesChangsha

Vibrio alginolyticus is one of the Vibrio pathogens common to humans and marine *** infection and induction of the host immune response,outer membrane proteins of bacteria play animportant *** this study,an outer membrane protein gene(ompW)was cloned from *** andexpressed in Escherichia *** 64. bp open reading frame(ORF)encodes a protein of 2.4.amino acidresidues with a predicted molecular weight of 2..3 *** amino acid sequence showed a high identitywith that of Photobacterium damselae(96.2.)and Vibrio parahaemolyticus(94.4.).The alignment analy-sis indicated that OmpW was highly *** dodecyl sulfate-polyacrylamide gel electrophoresisshowed that the gene was over-expressed in *** BL2.(DE3).Western blot analysis revealed that theexpressed protein had *** recombinant protein was purified by affinity chromatographyon Ni-NTA Superflow *** yellow croaker vaccinated with the purified OmpW showed significantlyincreased antibody to OmpW,which could resist the infection by ***.A specific antibody wasdetected by enzyme-linked immunosorbent *** study suggested that the conserved OmpW could bean effective vaccine candidate against infection by ***.

关键词： Vibrio alginolyticus ompW expression characterization immunogenicity vaccine candidate

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Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat

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Cell Research 2004年第2期14卷 161-168页

作者： Kai CHEN~(1,*), Dan LI~(1,*), Yu Hui JIANG~1, Wei Juan YAO~1, Xin Juan WANG~2. Xiao Chao WEI~2. Jing GAO~3, Li De XIE~1, Zong Yi YAN~4. Zong Yao WEN~(1**), Shu CHIEN~5 ~1Hemorheology Center, Department of Biophysics, School of Basic Medical sciences, Peking university, Beijing, 100083, China. ~2.epartment of Biochemical and Molecular Biology, School of Basic Medical sciences, Peking university, Beijing, 100083, China. ~3Department of Urology, Medical Center, New York university, USA. ~4.epartment of Mechanics and Engineering Science, Peking university, Beijing 100871, China. ~5Department of Bioengineering and Whitaker Institute of biomedical Engineering, university of California, San Diego, La Jolla, CA 92.93-04.2. USA 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 2.epartment of Biochemical and Molecular Biology School of Basic Medical Sciences Peking University Beijing 100083 China. 2.epartment of Biochemical and Molecular Biology School of Basic Medical Sciences Peking University Beijing 100083 China. 3Department of Urology Medical Center New York University USA. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 4.epartment of Mechanics and Engineering Science Peking University Beijing 100871 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 5Department of Bioengineering and Whitaker Institute of biomedical Engineering University of California San Diego La Jolla CA 92093-0412 USA

The cDNA fragment of human TRAIL (TNF-related apoptosis inducing ligand) was cloned into RevTet-On, a Tetregulated and high-level gene expression system. The gene expression system was constructed in a human leukemic cell line: Jurkat. By using RevTet-On TRAIL gene expression system in Jurkat as a cell model, we studied the influence of TRAIL gene on the changes of cellular apoptosis before and after the TRAIL gene expression, which was induced by adding tetracycline derivative doxycycline (Dox). The results indicated that the cellular apoptosis ratio was largely dependent on the TRAIL gene expression level. Moreover, it was found that the apoptosis-inducing TRAIL could cause significant changes in the biophysical properties of Jurkat cells. The cell surface charge density decreased, the membrane fluidity declined, the elastic coefficients K_I increased, and the proportion of α-helix in membrane protein secondary structure decreased. Thus, the apoptosis-inducing TRAIL gene caused significant changes on

关键词： TRAIL Tet gene expression system Jurkat apoptosis.

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Local connectivity of Julia sets for a family of biquadratic polynomials

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Science China Mathematics 2007年第10期50卷 1477-1492页

作者： Jing L~(1+) Wei-yuan QIU~2.~1 Department of Basic Education,Shanghai Maritime university,Shanghai 2.0000,China ~2.Department of Mathematics,{4. university,Shanghai 2.04.3,China 1. Department of Basic Education Shanghai Maritime University Shanghai 200000 China 2. Department of Mathematics Fudan University Shanghai 200433 China

The local connectivity of Julia sets for the family of biquadratic polynomials f_c(z)= (z^2.2.^2.z^2.with a parameter c is *** is proved that for any parameter c,the boundary of the immediately attracting domain of f_... 详细信息

关键词： biquadratic polynomials Julia sets local connectivity

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Control of anther cell differentiation and function by receptor-like protein kinases and transcriptional cascades

Control of anther cell differentiation and function by recep...

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2.11全国植物生物学研讨会

作者： Hong Ma School of Life sciences,Institute of Plant Biology,fudan {2.,Shanghai,2.04.3 China {4. of {4. sciences,fudan {2.,Shanghai,2.0032.China

In flowering plants,the male reproductive organ anther contains both reproductive and somatic *** reproductive cells undergo meiosis to produce haploid microspores,which further develop into pollen grains,whereas the somatic cell layers surround and support the reproductive cells and the subsequent *** of anther somatic cell layers is the tapetum,which is crucial for pollen development. Our previous molecular genetic studies indicate that several receptor-linked protein kinases,such as BAM1/2.and EMS 1,are required for normal differentiation of anther somatic cell *** experiments using molecular and cytological markers

关键词： Arabidopsis anther cell differentiation DYT1 receptor-like protein kinases transcriptomics

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