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Molecular cloning and expression profile of Dicer2 gene from black tiger shrimp （Penaeus monodon）

中国甲壳动物学会第十一届年会暨学术研讨会

作者： Haibo Jiang~(1*),Luyu Sun~1,Lujiao Gao~2,Erchao Li~1,Na Yu~1,Shengming Sun~1,Xiaoman Wen~1,Yueru Wang~1,Xueqin Jiang~1,Liqiao Chen~1 1.Department of Biology,east china Normal University,shanghai 200062 2.east china sea fisheries research institute,chinese academy of fishery sciences,shanghai 200090

A 10 weeks feeding experiment was conducted to investigate the effect of partial or complete substitution(0%,33%,66%,100%) of dietary soybean meal(SBM) with cottonseed meal(CSM) with or without lysine supplementation in diets on growth performance,feed utilization,and body composition of chinese mitten crab,Eriocheir *** crab juvenile with an initial average size of 1.62g were

关键词： Soybean meal Cottonseed meal Eriocheir sinensis Growth Digestibility

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Molecular cloning and expression profile of Dicer2 gene from...

Effects of water-borne copper on digestive and metabolic enzymes of giant freshwater prawn Macrobrachium rosenbergii

2012年中国水产学会学术年会

作者： Xiaolan Li 1,2 , Lishi Yang 1 , Wenjing Liu 1 , Jianhua Huang 1 , Falin Zhou 1 , Shigui Jiang 1,* (1 South china sea fisheries research institute, chinese academy of fishery sciences, Guangzhou 510300, china 2 College of Animal Science, South china Agriculture University, Guangzhou 510642, china)

Dicer is an key initiative protein of the RNA interference （RNAi） pathway that play an important role in shrimp viruses defense. In the present study, we identified and characterized a Dicer2 cDNA from black tiger shrimp Penaeus monodon （designated as PmDcr2）. The full length cDNA of PmDcr2 contained a 5’untranslated region （UTR） of 109 bp, an open reading frame （ORF） of 4509 bp and a 3’ UTR of 842 bp. The predicted PmDcr2 protein is171.657 KDa with the theoretical isoelectric point of 6.23. PmDcr2 amino acid shared the highest similarity of 92% and 91% with Litopenaeus vannamei and Marsupenaeus japonicas Dicer2, respectively. Phylogenic analysis shows PmDcr2 is clustering with Litopenaeus vannamei Dicer2 and Marsupenaeus japonicas Dicer2, and close to the group of Tribolium castaneum Dicer2 from insect. Real-time quantitative PCR showed that PmDcr2 was widely epressed in in almost all examined tissues except muscle, with high expression in gill, hemocyte and lymph. mRNA expression also revealed that PmDcr2 was up-regulated by Vibrio vulnificus and White Spot Syndrome Virus （WSSV） virus suspension, but not by Staphylococcus aureus in hepatopancreas. Furthermore, the viral nucleotide homologue dsRNA poly （I: C） and ssRNA R484 also remarkably induced PmDcr2 mRNA expression. These data reflect that PmDcr2 is a functional protein that referred to the immune response in black tiger shrimp.

关键词： Dicer2 Penaeus monndon RNAi pathway bacterial virus innate immunity

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Effects of water-borne copper on digestive and metabolic enz...

中国海洋湖沼学会第九次全国会员代表大会暨学术研讨会

作者： Na Li Yunlong Zhao Jian Yang (1.School of Life Science,east china Normal University,shanghai 200062,People’s Republic of china 2.Key Laboratory of Ecological Environment and Resources of Inland fisheries,Freshwater fisheries research Center,chinese academy of fishery sciences,Wuxi 214081,People’s Republic of china)

The aim of present study was to evaluate the potential utility of enzyme parameters as indicators of water-borne copper (Cu) contamination in the giant freshwater prawn Macrobrachium *** of the digestive enzymes of tryptase,pepsin,cellulase, amylase,and metabolic enzymes of alkaline phosphatase (AKP),acid phosphatase (ACP), superoxide dismutase (SOD) and glutathione-S-transferase (GST) were measured in the

关键词： Digestive enzymes Metabolic enzymes Copper Macrobrachium rosenbergii

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现场胃含物法测定鲐的摄食与生态转换效率

水产学报 2003年第3期27卷 245-250页

作者：孙耀于淼刘勇张波唐启升中国水产科学研究院黄海水产研究所中国水产科学研究院黄海水产研究所山东青岛　266071

2000年7月至9月在山东半岛南部沿海鲐繁殖海域的鱼类养殖网箱中,应用胃含物法测定了渤海优势鱼种鲐的摄食、生态转换效率等生态能量学参数。结果表明,(1)在饵料不受限制时,鲐的摄食量和生长呈不规则波浪式变化,其波距比较恒定,但波高变... 详细信息

2000年7月至9月在山东半岛南部沿海鲐繁殖海域的鱼类养殖网箱中,应用胃含物法测定了渤海优势鱼种鲐的摄食、生态转换效率等生态能量学参数。结果表明,(1)在饵料不受限制时,鲐的摄食量和生长呈不规则波浪式变化,其波距比较恒定,但波高变化较大;(2)鲐的瞬时胃含物重量随时间变化可用指数模型St=9.0876e-0.1868t描述,瞬时排空率Rt=0.1868g·(100g)-1·h-1(按湿重计算);(3)按Eggers公式可求得鲐日摄食量Cd=(15.60±2.55)g·(100g·d)-1(按湿重计算)或(69.84±11.42)kJ·(100g)-1·d-1;(4)实验期间鲐体重随时间的变化可用线性方程Wt=2.23t+16.71描述,由该式可求得其相对平均日生长量Gd=3.72g·(100g)-1·d-1或30.00kJ·(100g)-1·d-1;(5)由于已知的平均日摄食量和平均日生长量,可求得鲐生态转换效率Eg=23.85%(按湿重计算)或42.96%kJ。

关键词：鲐摄食生态转换效率胃含物法

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鱼类诺达病毒及其所导致的疾病

水产学报 2006年第6期30卷 831-836页

作者：黄剑南郭志勋冯娟林蠡中国水产科学研究院南海水产研究所广东省大亚湾水产试验中心

In recent years,piscine nodaviruses have emerged as major pathogens of a wide range of larval and juvenile marine finfish resulting in high mortality in aquaculture *** fish exhibit a range of neurological signs,such as erratic swimming behaviour with the associated microscopic lesions of necrosis and vacuolation of the central nervous tissues and *** round-shaped,unenveloped and 25-30 nm in diameter virus particles were found in the cytoplasm of affected retinal and nerve *** have a bipartite genome of positive-sense RNA,with RNA1 encoding the RNA-dependent RNA polymerase and RNA2 encoding the capsid *** RNA are capped,but not *** family Nodaviridae comprises two genera: Alphanodavirus and Betanodavirus,members of which primarily infect insects and fish,***,betanodavirus is also named piscine *** present,piscine nodaviruses are divided into four genotypes based on partial sequences of the coat protein *** and RT-PCR amplification have been developed as specific diagnostic methods for the detection of the *** to striped jack(Pseudocaranx dentex) nervous necrosis(SJNNV) were found in 65% of plasma samples collected from wild and domestic brood stocks of striped jack,suggesting that the virus is very *** antigens were detected in eggs,larvae,and ovaries of hatchery-reared and wild spawner fish,suggesting both horizontal and vertical modes of transmission of the *** of nodavirus-free spawners using ELISA for detection of antigens and RT-PCR techniques have successfully reduced incidences of the virus infections in juvenile sea bass(Dicentrarchus labrax),striped jack and barfin flounder(Verasper moseri).The SSN-1 and GF cell lines have been successfully used in isolating piscine *** there are many papers describing the molecular characteristics of betanodavirus,our knowledge of the genomic attributes of these viruses i

关键词：诺达病毒鱼类诺达病毒病毒性神经坏死症

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东海区拖网网囊网目选择性研究

水产学报 2005年第2期29卷 232-237页

作者：黄洪亮王明彦徐宝生张勋汤振明中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室上海 200090

通过东海区拖网网囊网目对带鱼和小黄鱼的选择性试验,对拖网最适网囊网目尺寸进行了分析研究。结果表明:1.网囊网目45 mm,小黄鱼和带鱼的逃逸率均较低,渔获中兼捕大量当年生经济幼鱼;2.网囊网目54 mm,小黄鱼的50%选择体长尚小于其优势... 详细信息

通过东海区拖网网囊网目对带鱼和小黄鱼的选择性试验,对拖网最适网囊网目尺寸进行了分析研究。结果表明:1.网囊网目45 mm,小黄鱼和带鱼的逃逸率均较低,渔获中兼捕大量当年生经济幼鱼;2.网囊网目54 mm,小黄鱼的50%选择体长尚小于其优势体长组范围,带鱼的50%选择体长已部分进入其优势体长组范围;3.网囊网目65mm,选择体长范围与选择鱼种的优势体长组范围基本一致。综合分析认为:目前东海区最理想的拖网网囊网目不小于60 mm。

关键词：网囊网目选择性拖网东海

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中国对虾病原菌(鳗弧菌)的研究

水产学报 1990年第1期14卷 1-7页

作者：郑国兴沈亚林李何中国水产科学院东海水产研究所中国水产科学院东海水产研究所上海

1987年7～10月福建省平谭县对虾养殖场发生了一起严重的流行病。病虾活动力减弱,食欲下降,个体消瘦,头胸甲心区附近呈白色或浅桔红色,血淋巴液稀薄、混浊、不能凝固。因病虾步足、游泳肢及尾扇呈鲜红色,被称为“红腿病”。死亡率可高达9... 详细信息

1987年7～10月福建省平谭县对虾养殖场发生了一起严重的流行病。病虾活动力减弱,食欲下降,个体消瘦,头胸甲心区附近呈白色或浅桔红色,血淋巴液稀薄、混浊、不能凝固。因病虾步足、游泳肢及尾扇呈鲜红色,被称为“红腿病”。死亡率可高达90％以上。从五只垂死病虾的心脏血淋巴液中分离到细菌作纯焙养,经肌肉注射和浸泡的人工感染方法,得到了与虾池中患病虾相同的症状。菌原菌是革兰氏阴性短杆鼠。以极生单鞭毛运动。发酵葡萄糖,产酸不产气。氧化酶、过氧化氢酶阳性。能还原硝酸盐成亚硝酸盐。精氨酸脱羧;鸟氨酸、赖氨酸不脱羧。V.P.阳性.在42℃中不能生长。不能在无盐和含有8％NaCl 的胨水中生长,但在含有3％和6％NaCl的胨水中生长良好。对弧菌抑制剂0/129敏感。经鉴定为鳗弧菌(Vibrio anguillarum Bergman)。

关键词：中国对虾鳗弧菌弧菌病

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带鱼人工授精和孵化

水产学报 2003年第2期27卷 188-192页

作者：万瑞景蒙子宁农业部海洋渔业资源可持续利用重点开放实验室农业部海洋渔业资源可持续利用重点开放实验室山东青岛　266071 中国水产科学研究院黄海水产研究所山东青岛　266071 厦门大学海洋系福建厦门　361005

The mature parent fish of Trichiurus lepturus was caught with bottom trawl on board R/V 'Beidou' in the Haizhou Bay, Yellow sea. The running eggs were artificially fertilized. The fertilized eggs began to divide in 1h30min, to be 16cell stage in 2h55min, late cleavage stage in 4h25min, blastula stage in 10h30min, closure of the blastopore in 25h45min, hatching in 78h45min after insemination, and the process of embryonic development was about 79h at 20.6-22.6℃.

关键词：带鱼授精孵化胚胎发育

中国对虾杂交优势对自然感染白斑综合征病毒的抗病力分析

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水产学报 2007年第1期31卷 68-75页

作者：李素红张天时孟宪红孔杰中国水产科学研究院黄海水产研究所中国水产科学研究院黄海水产研究所山东青岛266071 上海水产大学生命科学与技术学院上海200090

一个包括42个全同胞家系的中国对虾选育群体,荧光标记后每个家系各取40尾健康个体混养,自然发病并确认感染白斑综合征病毒(white spot-syndrome virus,W SSV)。发病1周后将所有个体取出,统计各家系的存活率,并对活体利用巢式聚合酶链式... 详细信息

一个包括42个全同胞家系的中国对虾选育群体,荧光标记后每个家系各取40尾健康个体混养,自然发病并确认感染白斑综合征病毒(white spot-syndrome virus,W SSV)。发病1周后将所有个体取出,统计各家系的存活率,并对活体利用巢式聚合酶链式反应(polymerasechain reaction,PCR)进行W SSV检测。对存活率进行分析,结果显示:4个家系的存活率在60%以上,7个家系的存活率在20%以下;存活率与第一次扩增阳性率、第二次扩增阳性率呈不显著负相关。第一次扩增阳性率与第二次扩增阳性率呈极显著正相关。结合两次扩增的阳性率分析得出:青岛野生群体内自交产生的一个家系和韩国群体养殖家系与乳山野生群体杂交产生的一个家系在抗病力上显著优于其它家系;经独立样本t检验表明,群体间交配组合的存活率差异不显著,因此家系选育可能是进行抗W SSV品种选育的重要途径。多重比较结果发现:韩国群体养殖家系(♂)与乳山野生群体(♀)杂交后代的存活率(56.20%±8.75%)显著高于韩国群体养殖家系自交后代(22.50%±2.50%)(P3.85)差异显著。

关键词：中国对虾巢式PCR 白斑综合征病毒

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魁蚶核型的研究

水产学报 1997年第4期21卷 455-457页

作者：周琳王子臣中国水产科学研究院东海水产研究所!上海200090 大连水产学院养殖系!116023

实验共计数魁蚶中期分裂相较好的细胞160个, 二倍体染色体数目是38条的细胞73个, 其百分率为45.6%。核型的基本形式是2n=38=10m+28sm, 染色体总臂数NF=76。

关键词：魁蚶染色体核型