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Characterization of a novel developmentally retarded mutant (drm1) associated with the autonomous flowering pathway in Arabidopsis

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Cell Research 2005年第2期15卷 133-140页

作者： Yong ZHU1,2,*, Hui Fang ZHAO1,3,*, Guo Dong REN1, Xiao Fei YU1, Shu Qing CAO1, Ben Ke KUAI1,4,**1department of Biochemistry, Ministry of Education Key laboratory for Biodiversity science and Ecological Engineering, school of life sciences, fudan university, 220 handan road, shanghai 200433, china 2school of Horticulture and Gardening, Southwest Forestry College, Kun Ming 650224, china 3department of biology, school of life and environment science, shanghai normal university, shanghai 200234, china4fudan centre for genetic diversity and designed agriculture, fudan university, 220 handan road, shanghai 200433, china department of Biochemistry Ministry of Education Key laboratory for Biodiversity Science and Ecological Engineering School of Life Sciences Fudan University Shanghai China school of Horticulture and Gardening Southwest Forestry College Kun Ming China department of biology School of Life and Environment Science Shanghai Normal University Shanghai China fudan centre for genetic diversity and designed agriculture Fudan University Shanghai China

A developmentally retarded mutant (drm1) was identified from ethyl methanesulfonate (EMS)-mutagenized M2 seedsin Columbia (Col-0) genetic background. The drm1 flowers 109 d after sowing, with a whole life cycle of about 160 *** also shows a pleiotropic phenotype, e.g., slow germination and lower germination rate, lower growth rate, curlingleaves and abnormal floral organs. The drm1 mutation was a single recessive nuclear mutation, which was mapped tothe bottom of chromosome 5 and located within a region of 20-30 kb around MXK3.1. There have been no mutantswith similar phenotypes reported in the literature, suggesting that DRM1 is a novel flowering promoting locus. Thefindings that the drm1 flowered lately under all photoperiod conditions and its late flowering phenotype was significantlyrestored by vernalization treatment suggest that the drm1 is a typical late flowering mutant and most likely associatedwith the autonomous flowering pathway. The conclusion was further confirmed by the revelation that the transcriptlevel of FLC was constantly upregulated in the drm1 at all the developmental phases examined, except for a very earlystage. Moreover, the transcript levels of two other important repressors, EMF and TFL1, were also upregulated in thedrm1, implying that the two repressors, along with FLC, seems to act in parallel pathways in the drm1 to regulateflowering as well as other aspects of floral development in a negatively additive way. This helps to explain why the drm1exhibits a much more severe late-flowering phenotype than most late-flowering mutants reported. It also implies that theDRM1 might act upstream of these repressors.

关键词： drm1 late-flowering autonomous flowering pathway Arabidopsis thaliana.

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