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Microglia activation in the offspring of prenatal Poly I:C exposed rats:a PET imaging and immunohistochemistry study

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General Psychiatry 2018年第4期31卷 31-38页

作者： Xue Li Xin Tian Luxian Lv Gangrui Hei Xufeng Huang Xiaoduo Fan Jinming Zhang Jianjiang Zhang Lijuan Pang Xueqin Song department of Nuclear Medicine Chinese PLA General HospitalBeijingChina The First Affliated Hospital/Zhengzhou UniversityZhengzhouChina henan province Biological Psychiatry Key Laboratory Xinxiang Medical UniversityXinxiangChina henan province Mental hospital The Second Affliated HospitalXinxiang Medical UniversityXinxiangChina School of Medicine and IHMRI University of WollongongWollongongNew South WalesAustralia UMass Memorial Medical Center University of Massachusetts Medical SchoolWorcesterMassachusettsUSA department of Nuclear Medicine Chinese PLA General HospitalBeijingChina

Background The well-known ‘pyrotherapy’ of Julius Wagner-Jauregg might be the beginning of the study on the immunological concepts of schizophrenia. As the primary immune effector cells in the brain, microglia play a pivotal role in neuroinfammatory processes. Maternal viral infection during pregnancy is associated with an increased risk for psychiatric disorders with presumed neurodevelopmental origin, including autism spectrum disorders and schizophrenia. The present study was to quantify microglia activation in vivo in the mature offspring of rats exposed to polyriboinosinic–polyribocytidilicacid （Poly I：C） during pregnancy using ^11C-PK11195 positron emission tomography （PET） and *** The study aimed to quantify microglia activation in vivo in the prefrontal cortex and hippocampus in mature offspring of prenatal Poly I：C exposed *** Offspring of Poly I：C-treated dams were the model group, offspring of saline-treated dams were the control group. Behavioural test for two groups was taken by spontaneous activity, prepulse inhibition （PPI） and latent inhibition （LI） test （including active avoidance conditioning task and passive avoidance conditioning task）. Randomly selected successful model rats were assessed by behavioural test in the model group and control group rats. 11C-PK11195 micro-PET/CT and immunohistochemistry were performed on the selected rats to measure microglia *** The treatment group showed hyperlocomotion and defcits in PPI and LI compared with the control group. The treatment group also showed an increased 11C-PK11195 uptake ratio in the prefrontal cortex （t=-3.990, p=0.003） and hippocampus （t=-4.462, p=0.001）. The number of activated microglia cells was signifcantly higher in the treatment group than in the control group （hippocampus： t=8.204, p〈0.001; prefrontal： t=6.995, p〈0.001）. Within the treatment group, there were signifcant correlations between the behavioural parameters and the activa

关键词： Microglia activation the offspring of prenatal Poly I C exposed rats a PET imaging and immunohistochemistry study

多层螺旋CT三维血管造影容积重建成像在颅内动脉瘤中的诊断价值

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实用放射学杂志 2005年第9期21卷 904-907页

作者：悦保仕温平贵河南省中医学院第一附属医院放射科周口市中心医院CT室河南郑州450000

目的探讨多层面螺旋CT三维血管造影(MS3D-CTA)容积重建(VR)技术在颅内动脉瘤中的诊断价值。方法对109例临床怀疑颅内动脉瘤的病人行MS3D-CTA和DSA检查。使用GELightspeedpro16层螺旋CT扫描仪获得原始图像,所有病例均采用VR技术对图像进... 详细信息

目的探讨多层面螺旋CT三维血管造影(MS3D-CTA)容积重建(VR)技术在颅内动脉瘤中的诊断价值。方法对109例临床怀疑颅内动脉瘤的病人行MS3D-CTA和DSA检查。使用GELightspeedpro16层螺旋CT扫描仪获得原始图像,所有病例均采用VR技术对图像进行三维重建,20例同时有最大密度投影(MIP)辅助检查。VR和MIP后处理图像以及DSA图像由位放射科医生用双盲法进行分析。结果DSA和手术证实83例共94个动脉瘤,其中单发74例,多发9例(7例2个动脉瘤,2例个动脉瘤)。VR图像上所测动脉瘤大小为1.3～32mm,与DSA比较无显著性差异(t=1.548,P>0.05)。VR对颅内动脉瘤的敏感度为96.8%,特异度为92.9%,准确度为95.9%,阳性预测值为97.8%,阴性预测值为89.7%;DSA敏感度为97.9%,特异度为963%,准确度为97.5%,阳性预测值为98.9%,阴性预测值为92.9%。94个动脉瘤中,89个(94.7%)动脉瘤在VR图像上能清晰显示瘤体、瘤颈与载瘤动脉的三维空间关系,DSA为57个(60.6%)。结论MS3D-CTAVR的敏感度、特异度和准确度均较高,是一种快捷、经济和有效的检查技术。

关键词：脑动脉瘤三维CT血管造影

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螺旋CT扫描技术在眼内异物诊断中的应用

眼外伤职业眼病杂志 2008年第8期30卷 604-605页

作者：谢伟吴强王辉付立郑州大学第一附属医院放射科河南郑州450052

目的探讨螺旋CT在眼内异物诊断中的应用价值。方法因眼内异物排查行螺旋CT检查共45例,除2例为双眼损伤外,其余为单眼病例。首选眼眶水平位扫描,必要时加冠状位扫描。扫描结果与手术结果做对比分析。结果发现眼内异物42例(44眼),其中金... 详细信息

目的探讨螺旋CT在眼内异物诊断中的应用价值。方法因眼内异物排查行螺旋CT检查共45例,除2例为双眼损伤外,其余为单眼病例。首选眼眶水平位扫描,必要时加冠状位扫描。扫描结果与手术结果做对比分析。结果发现眼内异物42例(44眼),其中金属异物30例(32眼),非金属异物12例(12眼),经螺旋CT定位的眼内异物经眼科手术全部成功摘出。结论螺旋CT扫描对眼内异物的检出及定位具有很大的临床应用价值。

关键词：螺旋CT 眼内异物

FUNCTION OF ADIPONECTIN AND TUMOR NECROSIS FACTOR-ALPHA IN PCOS WOMEN WITH INSULIN RESISTANCE

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FUNCTION OF ADIPONECTIN AND TUMOR NECROSIS FACTOR-ALPHA IN P...

The 5th International Conference on Reproductive Endocrinology

作者： Yang Xue-feng Ren Fen-ruo Gou Shu-ping department of Obstetrics and Gynecology,the Third affiliated hospital zhengzhou university,zhengzhou,china

Objective:To study the relationship between serum adiponectin (APN) and tumor necrosis factor-α (TNF-α) in polycystic ovarian syndrome (PCOS) women with insulin resistance. Method:Forty women with PCOS and twenty five healthy women were recruited and divided into 19 PCOS obese group (BMI≥25kg/m),21 PCOS non-obese group (BMI<25kg/m) and control group.

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Primary choriocarcinoma of the vagina in a non pregnant patient:a case report and review of the literature

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Primary choriocarcinoma of the vagina in a non pregnant pati...

2011中国妇产科学术会议暨浙江省计划生育与生殖医学学术年会暨生殖健康讲习班

作者： HAN Li-ping,HOU Na,ZHAO Li-juan,GENG Li-na,SHI Hui-rong department of Obstetrics and Gynecology,The first affiliated hospital of zhengzhou university,zhengzhou 450052, china

Objective To analyze diagnostic,clinical,therapeutic features in primary vaginal choriocarcinoma. Methods A case report with primary vaginal choriocarcinoma is reported and review of the literature was studied. Results A large vaginal mass was found in an 18-year-old *** serumβ-HCG level was markedly *** scan and colour Doppler ultrasonography revealed a hypervascular tumor in the vaginal *** examination of

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外用鱼油促进2型糖尿病大鼠伤口创面的愈合

陆军军医大学学报 2024年第6期46卷 535-543页

作者：黄荣江辛文彬倪向敏李正宙张贵明王建陆军军医大学(第三军医大学)第二附属医院营养科重庆400037

目的观察局部外用鱼油对2型糖尿病(diabetes mellitus type 2, T2DM)大鼠伤口创面愈合的影响,并探讨其作用机制。方法 57只SD大鼠通过高脂高糖饮食联合STZ腹腔注射建立T2DM大鼠模型,建模成功49只。T2DM大鼠模型建立成功后通过外科方法... 详细信息

目的观察局部外用鱼油对2型糖尿病(diabetes mellitus type 2, T2DM)大鼠伤口创面愈合的影响,并探讨其作用机制。方法 57只SD大鼠通过高脂高糖饮食联合STZ腹腔注射建立T2DM大鼠模型,建模成功49只。T2DM大鼠模型建立成功后通过外科方法创建伤口创面模型,采用随机数字表法分为鱼油组(n=17)、玉米油组(n=16)、模型对照组(n=16),各组于创面模型创建后次日开始给药,干预期间观察各组大鼠伤口创面愈合情况,分别于术后7、14 d取皮肤创面组织比较大鼠创面愈合情况,检测氧化应激指标(MDA、SOD、CAT)及炎症因子(TNF-α、IL-1β、IL-6、IL-10)水平,HE、Masson染色行病理学分析及观察皮肤真皮层胶原纤维沉积情况,Western blot检测SIRT1、Nrf2、NQO1蛋白表达。结果与玉米油组和模型对照组相比,术后第7、14天鱼油组大鼠伤口创面愈合率明显提升(P<0.05),胶原纤维沉积增多;创面组织MDA、TNF-α、IL-1β、IL-6水平明显下降(P<0.05),而SOD、CAT、IL-10水平显著升高(P<0.05);Western blot结果显示,鱼油组大鼠创面组织SIRT1、Nrf2、NQO1蛋白表达水平较玉米油组和模型对照组显著提升(P<0.05);干预期间玉米油组与模型对照组间愈合效果无显著差异。结论局部外用鱼油可有效促进T2DM大鼠伤口创面愈合,其机制可能与鱼油降低创面组织氧化应激及炎症反应有关。

关键词：鱼油糖尿病伤口氧化应激炎症反应沉默信息调节因子1(SIRT1)

Expression of Pdx-1 in bone marrow mesenchymal stem cells promotes differentiation of islet-like cells in vitro

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Science china(Life Sciences) 2006年第5期49卷 480-489页

作者： SUN Jiping1,YANG Yujia1,WANG Xiaoli1,SONG Jianhui1 & JIA Yanjie2 1.department of Pediatrics,Xiang-Ya hospital,Central-South university,Changsha 410008,china 2.department of Neurology,the first affiliated hospital,zhengzhou university,zhengzhou 450052,china department of Pediatrics Xiang-Ya Hospital Central-South University Changsha 410008 China department of Neurology the First Affiliated Hospital Zhengzhou University Zhengzhou 450052 China

Bone marrow mesenchymal stem cells (BMSCs) have the ability of self-renewaland multi-directional differentiation. Recent reports showed that BMSCs could differentiate intoendocrine cells of pancreas. However, the differentiation is not efficient enough to produceinsulin-producing cells for the future therapeutic use. Pdx-1 is a crucial regulator for pancreaticdevelopment. Therefore we constructed a eukaryotic expression vector containing Pdx-1 to determinethe effect of Pdx-1 expression on differentiation of BMSCs in vitro. The results showed that BMSCscould self-assemble to form functional pancreatic islet-like structures after differentiation invitro. The proportion of insulin-producing cells differentiated from Pdx-1 +BMSCs was 28.23%+-2.56%,higher than that from BMSCs transfected with vacant vector and Pdx-1'' BMSCs (7.23%+-1.56% and4.08%+-2.69% respectively) by flow cytometry. Immunocytochemical examination also testified theexpression of multiple bate-cells-specific genes such as insulin, glucagons, somatostatin indifferentiated BMSCs. The results also revealed that the expressions of genes mentioned above inPdx-1+BMSCs were higher than that in Pdx-VBMSCs, which was confirmed by Western blotting analysisand RT-PCR. Glucose-inducedinsulin secretion from Pdx-1+BMSCs in 5mmol/L and 25mmol/L glocuse was(56.61 +-4.82) uU/ml and (115.29+-2.56) uU/ml respectively, which were much higher than those fromPdx-1 BMSCs((25.53 +-6.49) uU/mL and (53.26 + 7.56) uU/mL respectively). Grafted animals were ableto maintain their body weight and survive for relatively longer periods of time than hyperglycemicsham-grafted controls, which demonstrated an overall beneficial effect of the grafted cells on thehealth of the animals. These findings thus suggested that exogenous expression of Pdx-1 shouldprovide a promising approach for efficiently producing islet-like cells from BMSCs for the futuretherapeutic use in diabetic patients.

关键词： bone marrow, mesenchymal stem cells, islet of Langerhans, pancreatic duodenal homeobox 1,diabetes.

Adipose derived stem cell-schwann cells: a novel and effective seed cell after contusion brain injury

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Adipose derived stem cell-schwann cells: a novel and effecti...

2011中华医学会神经外科学学术会议

作者： liang yang1 jiasheng fang2 ruokun chen4 wei wang3 daguang liao1 tianxiang huang2 1.department of neurosurgery, The third Xiangya hospital of Central South university, Changsha, Hunan, china 2.department of neurosurgery, Xiangya hospital of Central South university, Changsha, Hunan, china 3.department of neurology, The second Xiangya hospital of Central South university, Changsha, Hunan, china 4.department of neurology, the first affiliated hospital of zhengzhou university, zhengzhou, Hunan, china

Background Rapid development of tissue engineering techniques gives rise to the possibility of treating central nervous injuries with Schwann cells (SCs). However, some characteristics of the SC, such as its low proli... 详细信息

关键词： adipose derived stem cells motor cortex schwann cells seed cell transplantation

Transplanted ADSC-SCs Modify Reactive Gliosis after Contusion Brain Injury

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Transplanted ADSC-SCs Modify Reactive Gliosis after Contusio...

2011中华医学会神经外科学学术会议

Background Rapid development of tissue engineering techniques gives rise to the possibility of treating central nervous injuries with Schwann cells (SCs). Objective In the present study, we investigated whether transp... 详细信息

关键词： adipose derived stem cell neurocan reactive gliosis schwann cell transplantation