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Vein transplantation using human umbilical cord blood stem cells in the treatment of stroke sequela

Neural Regeneration Research 2006年第7期1卷 618-621页

作者： Yong Man1, Jianbin Li2, Bo Yang3, Ji Ma1 1department of Cerebral Surgery, the Second People’s hospital of zhengzhou, zhengzhou 450000, henan province, china 2Red Cross Blood Center of henan province, zhengzhou 450000, henan province, china 3department of neurosurgery, the first affiliated hospital of zhengzhou university, zhengzhou 450000, henan province, china department of Cerebral Surgery The Second People's Hospital of Zhengzhou Zhengzhou 450000 Henan Province China Red Cross Blood Center of henan province Zhengzhou 450000 Henan Province China department of neurosurgery Hospital of Zhengzhou University Zhengzhou 450000 Henan Province China

BACKGROUND: Transplanted mononuclear cell (MNC) of umbilical blood can survive in central nervous system (CNS) of host through blood brain barrier, differentiate into nerve cells, migrate to damaged site and integrate morphological structure and function with nerve cells of host so as to improve deficiencies of sensatory function, motor function and cognitive function and influence on stroke sequela. OBJECTIVE: To observe the vein transplantation of human umbilical cord blood stem cells (HUCBSC) for improving neurological function, limb function and activity of daily living of patients with stroke and evaluate the reliability. DESIGN: Self-controlled study. SETTING: department of neurosurgery, the Second People's hospital of zhengzhou City; Red-crossed Blood Center of henan province; department of neurosurgery, the Fist affiliated hospital of zhengzhou university. PARTICIPANTS: A total of 10 patients with stroke sequela were selected from department of Cerebral Surgery, the Second People's hospital of zhengzhou City from April to December 2005. There were 9 males and 1 female aged from 35 to 75 years with the mean age of 56 years. All of them were diagnosed with CT and MRI examination and coincidence with diagnostic criteria of stroke established by the Fourth National Academic Meeting for Cerebrovascular Disease. All patients provided informed consent. METHODS: 80-140 mL umbilical blood of term birth of newborn was selected hermetically and maintained in sterile plastic bag. And then, the blood was centrifugated at the speed of 1 500 r/min for 30 minutes at 22 ℃ in order to separate MNC, i.e., HUCBSC. In addition, after final diagnosis during hospitalization, stroke patients were perfused with HUCBSC through superficial vein of back of the hand. Each patient was averagely perfused with 6 portions of HUCBSC (cellular numbers ≥ 1×108/portion) and the interval between each portion was 1-7 days with the mean interval of 4 days. MAIN OUTCOME MEASURES: ① Neurological fun

关键词： cell stem Vein transplantation using human umbilical cord blood stem cells in the treatment of stroke sequela

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Human papillomavirus DNA and P16～(INK4A) expression in concurrent esophageal and gastric cardia cancers

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World Journal of Gastroenterology 2010年第46期16卷 5901-5906页

作者： Guang-Cheng Ding,Tao Guo,department of Gastroenterology,The first affiliated hospital and The Fifth affiliated hospital,zhengzhou university,zhengzhou 450052,henan province,china Jing-Li Ren,Xin Song,Sheng-Li Zhou,Zong-Min Fan,LiDong Wang,henan Key Laboratory for Esophageal Cancer Research,department of Gastroenterology,The first affiliated hospital,College of Basic Medicine,zhengzhou university,zhengzhou 450052,henan province,china Fu-Bao Chang,department of Thoracic Surgery,Linzhou Center hospital,Linzhou 456500,henan province,china Ji-Lin Li,department of Pathology,Yaocun Esophageal Cancer hospital,Linzhou 456500,henan province,china Ling Yuan,department of Oncoradiotherapy,henan province Tumor hospital,zhengzhou 450003,henan province,china Yi Zeng,Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052,china henan Key Laboratory for Esophageal Cancer Research Department of Gastroenterology The First Affiliated Hospital College of Basic Medicine Zhengzhou University Zhengzhou 450052 Henan Province China. ldwang2007@***

AIM:To investigate the relationship between human papillomavirus (HPV) infection and concurrent esophagus and gastric cardia cancer from the same patient (CC) and examine the significance of P16 INK4A protein ***:Polymerase chain reaction was used to detect the presence of HPV type16 (HPV16).The expression of P16 INK4A protein was detected using ***:Among the CC specimens,HPV16-DNA was found in eight cases of esophageal squamous cell carcinoma (ESCC) and five cases of gastric cardia adenocarcinoma (GCA),respectively (47% vs 29%),and two of both ESCC and GCA.P16 INK4A was highly expressed in both ESCC and *** the HPV-associated positive CC,higher P16 INK4A expression was observed in the GCA than in the ESCC (75% vs 25%,P < 0.05).CONCLUSION:HPV16 as a correlated risk factor may play an important role in the development of ESCC and GCA.P16 INK4A may be a screening index in the HPVassociated carcinoma of gastric cardia.

关键词： Esophageal squamous cell carcinoma Gastric cardia adenocarcinoma Human papillomavirus Polymerase chain reaction Immunohistochemistry

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Posterior Chamber IOL Implantation in Traumatic Cataract with Injured Complications

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眼科学报 1992年第3期8卷 111-112,125页

作者： Haike Guo Shiaofang Chang department of Ophthalmology,first Teaching hospital,henan Medical university zhengzhou 450000,china department of Ophthalmology First Teaching Hospital Henan Medical University Zhengzhou China.

Twenty-five cases of posterior chamber IOL implantation intraumatic cataract with complications associated with primary injury *** operating methods were described and the post-operative com-plications were ***-two percent of patients have the correctvision over 20/*** is suggested that the posterior chamber IOL can be im-planted in traumatic cataract with some injured *** SCIENCE1992;8:111-112.

关键词： traumatic cataract posterior chamber IOL implantation

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Management of Otitis Media with Effusion

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Journal of Otology 2008年第2期3卷 68-75页

作者： LIU Li-min, DONG Min-ming department of Otolaryngology, first affiliated hospital of zhengzhou university, zhengzhou, 450052 department of Otolaryngology First Affiliated Hospital of Zhengzhou university Zhengzhou 450052

Otitis media with effusion is one of the most common diseases in children. Its treatment remains controversial. Clinical practice guidelines of OME allow watchful waiting for 3 months before treatment if the child with OME is not at risk for speech/language/or learning problems. Tympanostomy tube insertion is the preferred initial procedure when a child becomes a surgical candidate. Complementary or alternative medicine is not recommended as a treatment for OME. This paper provides a systematic review of management of OME, which we hope will be helpful for clinicians.

关键词： Otitis media with effusion clinical protocols treatment

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In vitro Differentiation of Adipose-Derived Stem Cells into Hair Cell-Like Cells in Guinea Pigs

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Journal of Otology 2007年第2期2卷 97-101页

作者： WANG Ying1, DONG Min-min1, YANG Hai-bo2, GAO Song-tao3 1 department of Otolaryngology, the first affiliated hospital of zhengzhou university, china 2 department of Cardiology, the first affiliated hospital of zhengzhou university, china 3 department of orthopaedics, Xiangya hospital of Central South university, china department of Otolaryngology the First Affiliated Hospital of Zhengzhou University China department of Cardiology The First Affiliated Hospital of Zhengzhou University China department of orthopaedics Xiangya Hospital of Central South University China

Objective Adipose-derived stem cells(ADSCs) are suggested to possess a highly plastic ability to differentiate into several specific cell types in addition to adipocyte lineages, including germ layer tissue-specific cell lineages such as chondrocyte, myocyte, neuronal, and osteoblast lineages. The aim of this study is to establish an in vitro culture technique for ADSCs in an adult guinea pig model that facilitate their differentiation into hair cell-like cells. Materials and Methods Cells from inguinal fat pads in adult guinea pigs were cultured with β-mercaptoethanol, RA, Forskorin, Heregulin, bFGF, BDNF and EGF. Cellular differentiation was examined using immunocytochemistry techniques. Results The ADSCs demonstrated hair cell immunophenotypes with expression of epitopes of the hair cell marker protein myosin Ⅶa. Conclusion ADSCs from adult guinea pig adipose tissue can differentiate into hair cell-like cells when cultured in vitro. ADSCs may serve as seed cells for tissue engineering.

关键词： adipose-derived stem cell hair cell differentiate guinea pig

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Different ischemic preconditioning for rat liver graft: protection and mechanism

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Hepatobiliary & Pancreatic Diseases International 2003年第4期2卷 509-512页

作者： Shui-Jun Zhang Chang-Ju Zhu Yong-Fu Zhao Jie Li Wen-Zhi Guo the department of Surgery, first affiliated hospital, zhengzhou university, zhengzhou 450052, china department of Surgery First Affiliated Hospital Zhengzhou University Zhengzhou 450052 China. zhangshuijun@***

OBJECTIVE: To investigate the protective mechanism of different ischemic preconditioning (IPC) to ischemia/reperfusion (I/R) injury of rat liver graft. METHODS: 192 Wistar rats were randomly divided into 4 groups (48 rats in each group): control group (group C), experimental group 1 (group E_1), experimental group 2 (group E_2), and experimental group 3 (group E_3). IPC was not carried out in group C. In the experimental groups, IPC was carried out by blocking blood flow of the portal vein and hepatic artery and then reperfusion by removal of the clamp before donor liver was resected. Group E_1: 5-minute ischemia and 10-minute reperfusion; Group E_2: 5-minute ischemia and 5-minute reperfusion and one more the same procedure; Group E_3: 10-minute ischemia and 15-minute reperfusion. Four hours after IPC, liver transplantations were performed. Recipient blood and graft samples were obtained to determine the levels of ALT, AST, TNF-α and apoptosis index at 0.5, 2, 6, 24 hours after portal vein reperfusion. RESULTS: At 0.5, 2 hours after portal vein reperfusion, the levels of TNF-α in the experimental groups E_1, E_2, and E_3 were significantly lower than in the control group (P3 (P3 (P3 was significantly less than in the control group (P3(P3 was significantly less than in the control group (P<0.05). CONCLUSIONS: Ischemic preconditioning could attenuate liver graft injury by decreasing apoptosis of hepatocytes and production of TNF-α. The method of IPC with 5-minute ischemia, 5-minute reperfusion and one more the same procedure is a bette

关键词： rat liver transplantation apoptosis tumor necrosis factor α reperfusion injury liver graft

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Treatment of membranous Budd-Chiari syndrome: analysis of 480 cases

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Hepatobiliary & Pancreatic Diseases International 2004年第1期3卷 73-76页

作者： Pei-Qin Xu and Xiao-Wei Dang zhengzhou, china department of General Surgery, first A ffiliated Hospi- tal of zhengzhou university, zhengzhou 450052 , china department of General Surgery First Affiliated Hospital of Zhengzhou University Zhengzhou 450052 China. vl_institute1086@***

BACKGROUND: Budd-Chiari syndrome (BCS) presents a kind of disease resulted from the occlusion of hepatic vein and/or the intrahepatic inferior vena cava. Its different pathological types were proposed. According to our expe- rience , the membranous type takes a large part of it, and we tried to explore the best treatment of membranous BCS through the analysis of 480 cases retrospectively. METHOD: The operative results of 480 patients with mem- branous BCS were analysed retrospectively. RESULTS: Patients after Kimura's finger rupture, inter- ventional treatment and membrane resection were followed up with rates of 84.62%, 86.55%, and 87.37%, respective- ly. The effective rates of the three methods were 61.4%, 91.7%, and 90.4%, respectively, and the recurrence rates of the disease after the 3 procedures were 38.6%, 8.3% and 9.6%, respectively. The long-term effects of interventional treatment and resection were significantly better than those of Kimura' s finger rupture (P <0.05). CONCLUSION: Balloon dilatation is the choice for mem- branous BCS. Patients with extensive lesion, thick mem- brane or recurrence after percutaneous transhepatic angio- graphy should undergo membrane resection.

关键词： hepatic vein thrombosis balloon dilatation vascular surgical procedures Budd-Chiari syndrome

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Protective effects of glycine pretreatment on brain-death donor liver

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Hepatobiliary & Pancreatic Diseases International 2005年第1期4卷 37-40页

作者： Shui-Jun Zhang, Ji-Hua Shi, Zhe Tang, Yang Wu and Shi Chen zhengzhou, china department of Surgery, first affiliated hospital of zhengzhou university, zhengzhou 450052, china Institute of Organ Transplantation, Tongji Medical College , Huazhong university of Science and Technology, Wuhan 430030 , china department of Surgery First Affiliated Hospital of Zhengzhou University Zhengzhou 450052 China. zhangshuijin@***

BACKGROUND: Morphological and functional changes commonly occur in livers of brain-death donors. Preven- tion of liver injury from brain-death will benefit the results of transplantation. This study was conducted to evaluate the protection effects of glycine on the liver of brain-death donor. METHODS: Fourty-two male Wistar rats were randomly divided into brain-death donor (BDD) group (B), glycine pretreatment group with BDD (G), and strychnine pre- treatment group with BDD(S). For these groups, brain death model was established in donor rats and liver trans- plantation was performed subsequently utilizing microsur- gical techniques. After the establishment of the model and during cold rinsing of liver donors or liver reperfusion of recipients, glycine was given at a dose of 0. 6 mmol, 25 μmol and 25 μmol in the group G, and a same dose of gly- cine and strychnine (1000 :1) was prescribed for the group S, but nothing for the group B. Before cold rinsing at 2 and 6 hours after portal vein(PV) reperfusion, blood sam- ples were taken from infrahepatic vena cava (IHVC) to de- termine the levels of alanine aminotransferase (ALT), as- partate aminotransferase (AST), tumor necrosis factor al- pha (TNF-α) and hyaluronic acid (HA). At 6 hours after PV reperfusion, graft samples were fixed for morphological observation and apoptosis of hepatocytes was detected using the TUNEL method. RESULTS: Before liver cold rinsing and at 2 and 6 hours af- ter PV reperfusion, the serum levels of ALT, AST, TNF-α, HA and apoptosis index (AI) in the groups B and S were significantly higher than those in the group G (P 0.05). Electron microscopy showed that Kupffer cells were activated and hepatic cells injured more obvious- ly in the groups B and S than in the group G. CONCLUSION: Glycine pretreatment can improve the via-bility of the liver of the brain-death donor rat.

关键词： rats liver transplantation brain death glycine Kupffer cells donor

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Transgenic mice designed to express human α-1,2-fucosyltransferase in combination of human DAF and CD59 to avoid xenograft rejection

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Science china(Life Sciences) 2008年第3期51卷 199-204页

作者： LIU BingQian1, CHENG ChuanYu1, WU YuDong1, WEI JinXing1, LI GuangSan2 & MA TengXiang3 1 department of Urology, the first affiliated hospital of zhengzhou university, zhengzhou 450052, china 2 Institute of Genetics and Development Biology, Chinese Academy of Sciences, Beijing 100101, china 3 Tianjin Institute of Urology, Second hospital of Tianjin Medical university, Tianjin 300211, china department of Urology the First Affiliated Hospital of Zhengzhou University Zhengzhou 450052 China Institute of Genetics Development Biology Chinese Academy of Sciences Beijing 100101 China Tianjin Institute of Urology Second Hospital of Tianjin Medical University Tianjin 300211 China

The expression of human α-1,2-fucosyltransferase (HT) or complement regulatory proteins has been proved as an strategy to overcome hypercute rejection in discordant xenogeneic organ transplantation. In this study, we examined whether peripheral blood mononuclear cells (PBMCs) from polytransgenic mice expressing the human HT, and complement regulatory proteins (DAF and CD59), can provide more effective protection against xenograft rejection. Transgenic mice were produced by co-injection of gene constructs for human HT, DAF and/or CD59. Flow Cytometry (FCM) was used to screen the positive transgenic mice. PBMCs from transgenic mice were incubated with 15% human serum to evaluate natural antibody binding, complement activation and expression of adhesion molecules. Three transgenes were strongly expressed in PBMCs of transgenic mice, and HT expression signifi- cantly reduced expression of the major xenoepitope galactose-α-1,3-galactose (α-Gal). Functional studies with PBMCs showed that co-expression of HT and DAF or CD59 markedly increased their re- sistance to human serum-mediated cytolysis when compared with single transgenic PBMCs. Moreover, the combined expression of triple transgenes in PBMCs led to the greatest protection against human serum-mediated cytolysis, avoided hyperacute rejection and reduced expression of adhesion mole- cules. Strong co-expression of triple transgenes was completely protected from xenograft hyperacute rejection and partially inhibited acute vascular rejection. The studies suggest that engineering mice to express triple molecules represents an critical step toward prolonging xenograft survival and might be more suitable for xenotransplantation.

关键词： α-1 2-fucosyltransferase complement regulatory proteins galactose-α-1 3-galactose hyperacute rejection acute vascular rejection

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Surgical treatment of Budd-Chiari syndrome:analysis of 221 cases

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Hepatobiliary & Pancreatic Diseases International 2011年第4期10卷 435-438页

作者： Xiao-Wei Dang,Pei-Qin Xu,Xiu-Xian Ma,Da-Qian Xu,Yan-Ju Zhu and Yong-Shuai Zhang Division of Hepatobiliary and Pancreatic Surgery,first affiliated hospital,zhengzhou university,zhengzhou 450052,china Division of Hepatobiliary and Pancreatic Surgery First Affiliated Hospital Zhengzhou University Zhengzhou 450052 China

BACKGROUND:Budd-Chiari syndrome (B-CS) refers to post-hepatic portal hypertension and/or inferior vena cava hypertension caused by obstruction of blood flow at the portal cardinal hepatic *** treatments of B-CS include operations on pathological membrane lesions,shunting and combined *** have shown that china,Japan,India and South Africa have a high incidence of *** china,the Yellow River Basin in henan,Shandong,Jiangsu and Anhui provinces also have a high incidence,around 10 per 100 ***:The clinical data of 221 B-CS patients were analyzed *** focused on pathological types,surgical methods,effectiveness and complications of treatment,and ***:Based on imaging findings such as color ultrasonography,angiography or magnetic resonance angiography,the 221 patients were divided into 3 types (five subtypes):type Ia (72 patients),type Ib (20),type II (72),type IIIa (33),and type IIIb (24).Surgical procedures included balloon membranotomy with or without stent (65 patients),improved splenopneumopexy (18),radical resection of membrane and thrombus (17),inferior vena cava bypass [29,with cavocaval transflow (13) and cavoatrial transflow (16)],mesocaval shunt (41),splenocaval shunt (25),splenoatrial shunt (12),splenojugular shunt (6),and combined methods (8).The complication rate was 9.05% (20/221) and the perioperative death rate was 2.26% (5/221).All of the patients were followed up from 6 months to 5 *** success rate was 84.6% (187/221),and the recurrence rate was 8.9% (9/101) and 13.5% (13/96) after 1-and 5-year follow-up,***:The rational choice of surgical treatment based on B-CS pathological typing may increase the success rate and decrease the recurrence.

关键词： Budd-Chiari syndrome intervention shunt transflow

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