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Expression and function analysis of the metallo-thionein-like (MT-like) gene from Festuca rubra in Chlamydomonas reinhardtii chloroplast

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Science china(Life Sciences) 2008年第12期51卷 1076-1081页

作者： HAN SiHai1,2, HU ZhangLi1 & LEI AnPing1 1 institute of Eco-Environment, College of Life Science, shenzhen University, shenzhen 518060, china 2 Research Center for Analytical Instrumentation, State Key Lab of Industrial Control Technology, institute of Cyber-Systems and Control, Zhejiang University, Hangzhou 310058, china 1. institute of Eco-Environment College of Life Science Shenzhen University Shenzhen 518060 China 2. Research Center for Analytical Instrumentation State Key Lab of Industrial Control Technology Institute of Cyber-Systems and Control Zhejiang University Hangzhou 310058 China

The cDNA of the metallothionein-like (MT-like) gene from Festuca rubra cv. Merlin was optimized with bias codon of Chlamydomonous reinhardtii chloroplast genome. The optimized MT-like gene was de-livered into C. reinhardtii chloroplast and the transgenic strains expressing MT-like gene was obtained. PCR-Southern blot and RT-PCR-Southern blot analysis demonstrated that the MT-like gene was inte-grated into chloroplast genome of C. reinhardtii and expressed at the transcriptional level. The cad-mium binding capacity of the transgenic C. reinhardtii was determined by hydride generation-atomic fluorescence spectrometry (HG-AFS) and the binding properties were analyzed. Results showed that the transgenic C. reinhardtii expressing the MT-like gene exhibited remarkably higher Cd2+ binding capacity and grew to higher densities at toxic Cd2+ concentrations (40-100 μmol/L) than the wild type strain, and that the IC50 of Cd2+ (3-d treating ) to algal cell growth of transgenic strain was 55.43% higher than that of the wild type strain, indicating that the Cd2+ binding capacity and Cd2+ tolerance of C. reinhardtii was enhanced through the expression of the foreign MT-like gene in chloroplast.

关键词： Chlamydomonas reinhardtii chloroplast metallothionein-like (MT-like) gene cadmium binding properties

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Complete Resequencing of 40 Genomes Reveals Domestication Events and Genes in Silkworm（Bombyx）

Complete Resequencing of 40 Genomes Reveals Domestication Ev...

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第十五次全国动物遗传育种学术讨论会

作者： Qingyou Xia,~(1,2)*.Yiran Guo,~(3*)Ze Zhang,~(1,2*) Dong Li,~(1,3)*Zhaoling Xuan,~(3*) Zhuo Li,~(3*) Zhi Jiang,~3 Zhonghuai Xiang,~1 Jun Wang~3 1.The Key Sericultural Laboratory of Agricultural Ministry,College of Biotechnology,Southwest University,Chongqing 400715, china 2.institute of Agronomy and Life Sciences,Chongqing University,Chongqing 400044,china 3.beijing genomics institute at shenzhen,shenzhen 518083,china.~*These authors contributed equally to this work.

＜正＞Introduction The genetic variations can be analyzed by comparing the sequences of individuals or at the population level. whole-genome resequencing is considered to be the most efficient approach for studying ge... 详细信息

关键词： silkworm resequencing SNP domestication gene

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Assessment and adjustment of sea surface salinity products from Aquarius in the southeast Indian Ocean based on in situ measurement and My Ocean modeled data

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Acta Oceanologica Sinica 2016年第3期35卷 54-62页

作者： XIA shenzhen KE Changqing ZHOU Xiaobing ZHANG Jie Jiangsu Provincial Key Laboratory of Geographic Information Science and Technology Nanjing UniversityNanjing 210023China Collaborative Innovation Center of South china Sea Studies Nanjing 210023China Collaborative Innovation Center of Novel Software Technology and Industrialization Nanjing 210023China Department of Geophysical Engineering Montana Tech of the University of MontanaButteMT 59701USA The First institute of Oceanography State Oceanic AdministrationQingdao 266061China

The in situ sea surface salinity（SSS） measurements from a scientific cruise to the western zone of the southeast Indian Ocean covering 30°-60°S, 80°-120°E are used to assess the SSS retrieved from Aquarius（Aquarius SSS）.Wind speed and sea surface temperature（SST） affect the SSS estimates based on passive microwave radiation within the mid- to low-latitude southeast Indian Ocean. The relationships among the in situ, Aquarius SSS and wind-SST corrections are used to adjust the Aquarius SSS. The adjusted Aquarius SSS are compared with the SSS data from My Ocean model. Results show that：（1） Before adjustment： compared with My Ocean SSS, the Aquarius SSS in most of the sea areas is higher; but lower in the low-temperature sea areas located at the south of 55°S and west of 98°E. The Aquarius SSS is generally higher by 0.42 on average for the southeast Indian Ocean.（2） After adjustment： the adjustment greatly counteracts the impact of high wind speeds and improves the overall accuracy of the retrieved salinity（the mean absolute error of the Zonal mean is improved by 0.06, and the mean error is-0.05 compared with My Ocean SSS）. Near the latitude 42°S, the adjusted SSS is well consistent with the My Ocean and the difference is approximately 0.004.

关键词： Aquarius sea surface salinity(SSS) in situ SSS My Ocean comparison analysis southeast Indian Ocean

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Improvement of Efficiency and Flexibility in Multi-slice Helical CT

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Journal of Shanghai Jiaotong university(Science) 2008年第4期13卷 408-412页

作者：孙文武陈思平庄天戈 Nanchang institute of Aeronautical Technology shenzhen University Department of Biomedical Engineering Shanghai Jiaotong University

One of the main aspects in computed tomography (CT) development is to make CT rapidly scan a large longitudinal volume with high z-axis resolution. The combination of helical scanning with multi-slice CT is a promising approach. Image reconstruction in multi-slice CT becomes, therefore, the major challenge. Known algorithms need to derive the complementary data or work only for certain range of pitches. A reconstruction algorithm was presented that works with the direct data as well as arbitrary pitches. Filter interpolation based on the proposed method was implemented easy. The results of computer simulations under kinds of conditions for four-slice CT were presented. The proposed method can obtain higher efficiency than the conventional method.

关键词： multi-slice computed tomography helical interpolation rebinning filter interpolation

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RESEARCH ON THE POPULATION CARRYING CAPACITY OF THE LAND RESOURCES IN THE ECONOMIC AREA OF ZHUJIANG DELTA

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Chinese Geographical Science 2001年第2期11卷 174-180页

作者： TANG Hui-jun1, JIANG Jian-quan2 (1.institute of Economy Management, Guangdong University of Technology, Guangzhou 510090, P.R.china 2.shenzhen Longgang Urban Planning ＆Architecture institute, shenzhen, 518172, P.R.china) institute of Economy Management Guangdong University of Technology Guangzhou P. R. China shenzhen Longgang Urban Planning & Architecture institute Shenzhen P. R. China

The authors once made a preliminary research on population carrying capacity of the land in the Economic Area of Zhujiang Delta (EAZD for short) in 1995, and reckoned that the ultimate population in this region will be 23 550 thousand by year of 2000. While the population in being in EAZD was 22.62 million in *** accords with the prefigured result in the rough from the point of view of development. According to the data of plow land resources from the 2000 Statistical Yearbook of EAZD and the study on the population-foodstuff-plow land relationship, this paper calculates the productive potential of plow land and the population carrying capacity of land by year of 2010, and puts forward the countermeasures for improving the population carrying capacity of land in this region.

关键词： land resources land production potential population carrying capacity of land the Economic Area of Zhujiang Delta

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Application of genomics Research in Plant

Application of Genomics Research in Plant

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第十二届全国植物基因组学大会

作者： Bicheng Yang,Yue Cai~* Bgishenzhen,shenzhen 518083,china

In recent years,next-generation sequencing platforms have become widely available,dramatically improving the throughput and reducing the cost of DNA *** goal of genomics research is to develop a basic knowledge of the structures and functions of plant *** has developed the cucumber,watermelon, Chinese cabbage,tea plant,orchid and potato genomes using genome-wide shotgun strategy and mainly incorporating Illumina sequencing technologies,and are actively working on more than 70 plant

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含有理想导体的准分形结构光子晶体的能带

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物理学报 2004年第9期53卷 3205-3210页

作者：李岩郑瑞生冯玉春牛憨笨中国科学院西安光学精密机械研究所中国科学院研究生院北京100864 深圳大学光电子学研究所广东省光电子器件与系统重点实验室深圳518060

用时域有限差分方法计算了一组具有相似几何结构且包含理想金属材料的准分形光子晶体的能带 .数值计算结果表明 ,这种准分形结构光子晶体具有绝对带隙 ,且带隙的宽度会随着分形级数的增大而增大 .同时 ,随着级数的增大 ,其能带在整体地... 详细信息

用时域有限差分方法计算了一组具有相似几何结构且包含理想金属材料的准分形光子晶体的能带 .数值计算结果表明 ,这种准分形结构光子晶体具有绝对带隙 ,且带隙的宽度会随着分形级数的增大而增大 .同时 ,随着级数的增大 ,其能带在整体地趋向于高频端的同时。

关键词：光子晶体带隙时域有限差法分形级数能带结构

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The Difference in Cognition Consistency Between the Sciences and Humanities

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genomics, Proteomics & Bioinformatics 2003年第4期1卷 243-246页

作者： Jianzhang ZhouDepartment of Philosophy, Xiamen University, Xiamen 361005, china.Xin Zhang (beijing genomics institute, beijing101300,china) Department of Philosophy Xiamen University Xiamen 361005 China

It is reasonable that the strict sciences represented by mathematics, physics and chemistry have nearly been a kind of substitutional belief of humans after "the Death of God". To conclude theoretically, the significant attraction or the extremely great power of science is, in a word, the validity of thinking and action. From the viewpoint of human action or prac-

关键词： in on it or of that The Difference in Cognition Consistency Between the Sciences and Humanities

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OCDMA Network and Key Technology

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光学学报 2003年第S1期23卷 721-722页

作者： Li Yuquan, Pu Tao, Xu Ming, Yang Shuwen PLA University of Science and Technology, institute of Communication Engineering, Nanjing, Jiangsu P. R. china,Tel: 86-25-3384990, Fax: 86-25-3384990, Email: proflyq@*** shenzhen University, Advanced Technology Research Centre, shenzhen, Guangdong, P.R.china PLA University of Science and Technology Institute of Communication Engineering Nanjing Jiangsu P. R. China shenzhen University Advanced Technology Research Centre Shenzhen Guangdong P.R.China

OCDMA's network application on LAN, metro-ring and backbone networks are discussed. Its simple tell-go protocol makes it a powerful competitor with CSMA/CD in optical LANs. Its large soft capacity could erase the wavelength routing algorithm of WDM based metro-ring. And its content susceptibility could be largely used in label switching backbone network. And why should be 2D CODEC as well as interference cancellation is although mentioned as OCDMA key technology.

关键词： OCDMA Network and Key Technology be of as in length on WDMA CSMA

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Cloning and expression of putative ethylene receptor genes in soybean plant

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Progress in Natural Science:Materials International 2007年第10期17卷 1152-1160页

作者： Xie Zongming1,2,3, Lei Gang1,2, Wuriyanghan Hada1,2, Tian Aiguo1, Zhang Jinsong1 and Chen Shouyi1(1. National Key Laboratory of Plant genomics, institute of Genetics and Developmental Biology, Chinese Academy of Sciences, beijing 100101, china 2. Graduate School of Chinese Academy of Sciences, beijing 100039, china 3. Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, china) National Key Laboratory of Plant genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Graduate School of Chinese Academy of Sciences Beijing 100039 China Xinjiang Academy of Agricultural and Reclamation Science Shihezi 832000 China

Ethylene plays important roles in plant growth, development, and stress responses, and ethylene receptors have been identified and studied extensively in various plant species. Here we report the cloning of four ethylene receptor genes from soybean, i.e. GmETR1, GmERS1, GmETR2 and GmEIN4. Construction of the phylogenic tree showed that GmETR1 and GmERS1 belong to subfamily I whereas GmETR2 and GmEIN4 belong to subfamily II. The four ethylene receptor genes showed different tissue-specific expression patterns in roots, stems, leaves, cotyledons, flowers, pods and seeds of soybean. These genes were differentially regulated by various abiotic stresses and plant hormones. The possible roles of the four genes in soybean plant were also discussed.

关键词： abiotic stress expression analysis ethylene receptor plant hormone soybean.

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