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Sequencing,annotation and comparative analysis of nine BACs of the giant panda(Ailuropoda melanoleuca)

Science china(Life Sciences) 2010年第1期53卷 107-111页

作者： ZHENG Yang1,2,3,CAI Jing4,LI JianWen3,6,LI Bo3,LIN RunMao3,TIAN Feng3,WANG XiaoLing3 & WANG Jun3,5 1beijing institute of genomics,Chinese Academy of Sciences(CAS),beijing 100029,china 2College of Life Science,Graduate University of Chinese Academy of Sciences,beijing 100049,china 3beijing genomics institute at shenzhen,shenzhen 518083,china 4CAS-Max Planck Junior Research Group on Evolutionary genomics,State Key Laboratory of Genetic Resources and Evolution,Kunming institute of Zoology,Chinese Academy of Sciences(CAS),Kunming 650223,china 5Department of Biology,University of Copenhagen,Copenhagen DK-2200,Denmark 6School of Biological Science and Engineering,South china University of Technology,Guangzhou 510006,china beijing institute of genomics Chinese Academy of Sciences (CAS) Beijing China College of Life Science Graduate University of Chinese Academy of Sciences Beijing China beijing genomics institute at shenzhen Shenzhen China CAS-Max Planck Junior Research Group on Evolutionary genomics State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences (CAS) Kunming China School of Biological Science and Engineering South China University of Technology Guangzhou China Department of Biology University of Copenhagen Copenhagen Denmark

A 10-fold BAC library for the giant panda was constructed and nine BACs were selected to generate finish *** BACs could be used as a validation resource for the de novo assembly accuracy of the whole genome shotgun sequencing reads of the giant panda newly generated by Illumina GA sequencing *** Sanger sequencing,assembly,annotation and comparative analysis were carried out on the selected BACs of a joint length 878 *** search and de novo prediction methods were used to annotate genes and *** protein coding genes were predicted,seven of which could be functionally *** seven genes have an average gene size of about 41 kb,an average coding size of about 1.2 kb and an average exon number of 6 per ***,seven tRNA genes were *** 27 percent of the BAC sequence is composed of repeats.A phylogenetic tree was constructed using a neighbor-join algorithm across five species,including the giant panda,human,dog,cat and mouse,which reconfirms dog as the most closely related species to the giant *** results provide detailed sequence and structure information for new genes and repeats of the giant panda,which will be helpful for further studies about the giant panda.

关键词： giant panda BAC sequencing assembly annotation evolution

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Sequencing and analysis of the giant panda genome

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Science china(Life Sciences) 2010年第8期53卷 1047-1048页

作者： YANG HuanMing BGI-shenzhen (beijing genomics institute shenzhen), shenzhen 518083, china 1. BGI-shenzhen (beijing genomics institute shenzhen) Shenzhen 518083 China

The giant panda (Ailuropoda melanoleuca) is loved all over the world and is considered a symbol of china, as illustrated by its being one of the

关键词： Sequencing and analysis of the giant panda genome

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Antisense expression of a rice cellular apoptosis susceptibility gene (OsCAS} alters the height of transgenic rice

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Progress in Natural Science:Materials International 2007年第1期17卷 39-43页

作者： XU Chunxiao and HE Chaozu ( National Key Laboratory of Plant genomics, institute of Microbiology, Chinese Academy of Sciences, beijing 100080, china Graduate University of Chinese Academy of Sciences, beijing 100039, china shenzhen Laboratory of Superhybrid Rice Research, Graduate School at shenzhen, Tsinghua University, shenzhen 518055, china) [a]National Key Laboratory of Plant genomics Institute of Microbiology Chinese Academy of Sciences Beijing China [b]Graduate University of Chinese Academy of Sciences Beijing China

Cellular apoptosis susceptibility (CAS) gene plays important roles in mitosis, development and export of importin a from the nucleus, but its function in plant is unknown. In this study, a rice CAS ortholog (OsCAS), which encodes a predicted protein of 983 amino acids with 62% similarity to human CAS, was identified. DNA gel blot analysis revealed a single copy of OsCAS in the rice genome. A 973 bp fragment at the 3' end of OsCAS cDNA was cloned from rice cDNA library and transferred into rice in the antisense direction under the control of CaMV 35S promoter via Agrobacterium-mediated transformation method, 105 transgenic lines were obtained. Expression of OsCAS was suppressed in the antisense transgenic lines as revealed by semi-quantitative RT-PCR. The antisense transgenic lines showed dwarf phenotypes. The results indicated that OsCAS was involved in culm development of rice.

关键词： antisense dwarf ortholog OsCAS rice.

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广东深圳长圳公共租赁住房新型建造技术集成项目

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建筑实践 2024年第3期 104-111页

作者： china Construction Science Technology Group Co., Ltd. shenzhen General institute of Architectural Design and Research Co., Ltd. 中建科技集团有限公司深圳市建筑设计研究总院有限公司

长圳公共住房及其附属工程项目（以下简称长圳项目）位于深圳市光明区,是深圳市规模最大的装配式公共住房项目和深圳市建设管理模式改革创新的试点项目。项目总投资58亿元,地上建筑24栋,总建筑面积约115万m2,其中:住宅建筑面积81万m2,...

长圳公共住房及其附属工程项目（以下简称长圳项目）位于深圳市光明区,是深圳市规模最大的装配式公共住房项目和深圳市建设管理模式改革创新的试点项目。项目总投资58亿元,地上建筑24栋,总建筑面积约115万m2,其中:住宅建筑面积81万m2,商业建筑6.5万m2,公共配套设施3.2万m2,多层建筑高度10—24m,高层建筑高度100—150m,提供近万套人才安居住房。

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Positioning localities from spatial assertions based on Voronoi neighboring

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Science china(Technological Sciences) 2010年第S1期53卷 143-149页

作者： GONG YongXi 1,2,3,LI GuiCai 1,LIU Yu 2 & YANG Jian 1 1 shenzhen Key Lab of Recycling Economy,Peking University shenzhen Graduate School,shenzhen 518055,china 2institute of Remote Sensing and Geographical Information Systems,Peking University,beijing 100871,china 3College of Urban and Environmental Science,Peking University,beijing 100871,china Peking Univ Shenzhen Grad Sch Shenzhen Key Lab Recycling Econ Shenzhen 518055 Peoples R China Peking Univ Inst Remote Sensing & Geog Informat Syst Beijing 100871 Peoples R China Peking Univ Coll Urban & Environm Sci Beijing 100871 Peoples R China

With the rapid development of Internet,much spatial information contained in non-structured or semi-structured documents is available on the World Wide *** such documents,localities are always textually described using spatial relationships and named places,instead of numerical ***,extracting positional information from locality descriptions is an important *** this paper,we bridge two aspects of locality descriptions,namely generating locality descriptions and positioning localities,and provide a method to compute probability density according to the selection probability of a reference object to describe the position of the target *** operation on uncertainty field is used to deal with locality description involving multiple reference *** metrics are introduced to measure the results of positioning *** choose the mixed selection probability function based on Euclidean distance and Voronoi stolen-area to compute probability density ***,we use three cases to demonstrate the proposed methods.

关键词： geographic information science positioning locality Voronoi neighboring probability density function

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LONG TIME ASYMPTOTIC BEHAVIOR OF SOLUTION OF DIFFERENCE SCHEME FOR A SEMILINEAR PARABOLIC EQUATION (Ⅱ)

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Journal of Computational Mathematics 1998年第5期16卷 571-576页

作者： Hui Feng(shenzhen University Normal College, shenzhen 518060, china Laboratorp of Computational Physics, institute of Applied Physics and Computational Mathematics,P.O.Box 8009, beijing 100088, china )Long-jnn Shen(Laboratory of Computational Physics, In shenzhen Univ Wormal Coll Shenzhen 518060 Peoples R China Inst Appl Phys & Computat Math Lab Computat Phys Beijing 100088 Peoples R China

In this paper we prove the solution of explicit difference scheme for a semilinear parabolic equation converges to the solution of difference scheme for the relevant nonlinear stationary problem as t→∞. For nonlinear parabolic problem, we obtain the long time asymptotic behavior of its discrete solution which is analogous to that of its continuous solution. For simplicity, we discuss one-dimensional problem.

关键词： Asymptotic behavior Explicit difference scheme Semilinear parabolic equation.

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Genomic research for important pathogenic bacteria in china

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Science china(Life Sciences) 2009年第1期52卷 50-63页

作者： YANG RuiFu1,GUO XiaoKui2,YANG Jian3,JIANG YongQiang1,PANG Bo4,CHEN Chen5,YAO YuFeng2,QIN JinHong2 & LI QingTian2 1 Laboratory of Analytical Microbiology,State Key Laboratory of Pathogen and Biosecurity,beijing institute of Microbiology and Epidemiology,beijing 100071,china 2 Department of Medical Microbiology and Parasitology,institutes of Medical Sciences,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,china 3 State Key Laboratory for Molecular Virology and Genetic engineering,institute of Pathogen Biology,Chinese Academy of Medical Sciences,beijing 100176,china 4 State Key Laboratory for Infectious Disease Prevention and Control,institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,beijing 102206,china 5 beijing genomics institute at shenzhen,shenzhen 518083,china 1. Laboratory of Analytical Microbiology State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 100071 China 2. Department of Medical Microbiology and Parasitology Institutes of Medical Sciences Shanghai Jiao Tong University School of Medicine Shanghai 200025 China 3. State Key Laboratory for Molecular Virology and Genetic engineering Institute of Pathogen Biology Chinese Academy of Medical Sciences Beijing 100176 China 4. State Key Laboratory for Infectious Disease Prevention and Control Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China 5. beijing genomics institute at shenzhen Shenzhen 518083 China

Rapid accumulation of bacterial genomic data offered an unprecedented opportunity to understand bacterial biology from a holistic view of *** can thus closely look at the way in which a pathogen is evolved,and these data has been applied to molecular epidemiology and microbial forensics,and screening of novel diagnostic,vaccine and drug *** newly developed high-throughput low-cost sequencing technologies,such as 454,Solexa and SOLiD,will promote the acquisition and application of genomic data in new research areas that we dared not imagine previously,such as the metagenomics of human gastric-intestinal tract,for better and comprehensive understanding of human health and disease.

关键词： genome pathogenic bacteria sequencing application

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Generation of pancreatic islet cells from human embryonic stem cells

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Science china(Life Sciences) 2009年第7期52卷 615-621页

作者： ZHANG DongHui1,2,JIANG Wei1,SHI Yan2 & DENG HongKui1,2 1 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education,College of Life Sciences,Peking University,beijing 100871,china 2 Laboratory of Chemical genomics,shenzhen Graduate School of Peking University,shenzhen 518055,china Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education College of Life Sciences Peking University Beijing 100871 China Laboratory of Chemical genomics Shenzhen Graduate School of Peking University Shenzhen 518055 China

Efficiently obtaining functional pancreatic islet cells derived from human embryonic stem(hES) cells not only provides great potential to solve the shortage of islets sources for type I diabetes cell therapy,but also benefits the study of the development of the human pancreas and diabetes *** 2001,hES cells were reported to have the capacity to generate insulin-producing cells by spontaneous differentiation in *** then,many strategies(such as overexpression of key transcription factors,delivery of key proteins for pancreatic development,co-transplantation of differentiated hES cells along with fetal pancreas,stepwise differentiation by mimicking in vivo pancreatic development) have been employed in order to induce the differentiation of pancreatic islet cells from hES ***,patient-specific induced pluripotent stem(iPS) cells can be generated by reprogramming somatic *** cells have characteristics similar to those of ES cells and offer a new cell source for type I diabetes cell therapy that reduces the risk of immunologic *** this review,we summarize the recent progress made in the differentiation of hES and iPS cells into functional pancreatic islet cells and discuss the challenges for their future study.

关键词： human embryonic stem(hES) pancreatic islet cells differentiation induced pluripotent stem(iPS) cells

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The performance of ultra wideband acquisition system based on energy detection over IEEE 802.15.3a channel

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Science china(Information Sciences) 2011年第2期54卷 360-370页

作者： YANG ZhiHua, ZHANG QinYu, ZHANG NaiTong & WANG Ye Communication Engineering Research Center, shenzhen Graduate School, Harbin institute of Technology, shenzhen 518055, china 1. Communication Engineering Research Center Shenzhen Graduate School Harbin Institute of Technology Shenzhen 518055 China

In this paper, we propose a theoretical framework to analyze the performance of ultra wideband acquisition (UWB) system based on energy detection (ED) over IEEE 802.15.3a channel. The proposed framework enables to calculate probability density function (PDF) of square-sum of multipath components (MPCs) gain collected by receiver, and the averaged criteria. In particular, the expectation and variance of the sum variant characterized approximately by log-normal distribution are expressed in a closed form based on cluster point process. Moreover, the calculating methods of averaged criteria, through Markov chain model and signal flow graph, are clearly demonstrated for different applicable scenarios. The proposed framework is well consistent with simulation results with respect to each calculation method.

关键词： UWB energy detection unconditional criterion cluster point process

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Optical characteristics and environmental pollutants detection of porous silicon microcavities

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Science china Chemistry 2011年第8期54卷 1348-1356页

作者： HUANG JianFeng, LI Sha, CHEN QingWei & CAI LinTao CAS Key Laboratory of Health Informatics shenzhen Key Laboratory of Cancer Nanotechnology institute of Biomedical and Health Engineering, shenzhen institute of Advanced Technology, Chinese Academy of Sciences, shenzhen 518055, china 1. CAS Key Laboratory of Health Informatics shenzhen Key Laboratory of Cancer Nanotechnology institute of Biomedical and Health Engineering Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 China

Porous silicon microcavities (PSM) optical crystals consisting of a Fabry-Perot microcavity embedded between two distributed Bragg reflectors have been fabricated by electrochemical etching. Scanning electron microscopy (SEM) clearly depicted their physical sandwich construction. The optical feature of the PSM structure was tuned by varying the anodization parameters. Through proper thermal oxidation and surface chemical modifications, the resulting structures were employed as optical sensors for the detection of environmental pollutants including volatile organic vapors (i.e. acetonitrile, toluene, cyclohexane, chloroform, acetone and ethanol) and interior decoration gases (i.e. toluene, ammonia and formaldehyde). Fourier transform infrared spectroscopy (FTIR) spectra confirmed the effective thermal annealing and surface modification chemistry, and the sensing process was accompanied by recording the modified structures' optical responses when exposed to target analytes. The PSM optical sensors showed good stability, sensitivity and selectivity, implying promising applications in gas sensing and en- vironmental monitoring.

关键词： porous silicon microcavity optical sensor thermal oxidation surface modification environmental pollutants detection

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