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Anticancer effect of two diterpenoid compounds isolated from Annona glabra Linn

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Acta Pharmacologica Sinica 2004年第7期25卷 91-96页

作者： Yong-hong ZHANG2, Hai-yan PENG3, Guo-hao XIA3, Ming-yan WANG3, Ying HAN4 First Clinical college 3Basic Medicine college, nanjing University of traditionalChinese Medicine, nanjing 2.002. 4Jiangsu Institute of Materia Medica, nanjing 210009, china First Clinical college Basic Medicine college Nanjing University of Traditional Chinese Medicine Jiangsu Institute of Materia Medica

AIM: To study the inhibitory effect of two diterpenoid compounds isolated from Annona glabra Linn (Cunabic acid and ent-kauran-19-al-17-oic acid) on the proliferation of Human Liver Cancer (HLC) cell line SMMC-7721 and its mechanism. METHODS: Inhibition of cell proliferation was measured by MTT assay. The morphological changes of SMMC-7721 cells were observed under inverted phase-contrast microscope, fluorescent microscope, transmission electron microscope (TEM), and scanning electron microscope (SEM). Flow cytometer (FCM) was used to calculate the cell apoptotic rate, and immunohistochemical staining was used to observe the regulation of gene expression. RESULTS: The proliferation of SMMC-7721 cells was obviously inhibited after being treated with Cunabic acid at the concentration >5 μmol/L and ent-kauran-19-al-17-oic acid >10 μmol/L. The biggest inhibitory effect was 81.05 % when treated with Cunabic acid at the concentration of 25 μmol/L. The effect had a linear relationship with concentration. The result indicated that drug-treated cells exhibit typical morphological changes of apoptosis, including condensed chromatin and a reduction in volume. Sub-G0/G1 peak was found by FCM analysis and the cell cycle was arrested at G0/G1 stage. The apoptotic rates of the cells treated by Cunabic acid and ent-kauran-19-al-17-oic acid were 43.31 % and 24.95 %, respectively. It was visualized by immunohis- tochemical staining that the drugs down-regulated the gene expression of bcl-2 gene and up-regulated that of bax gene. CONCLUSION: The two diterpenoid compounds isolated from Annona glabra Linn, Cunabic acid and ent- kauran-19-al-17-oic acid can obviously inhibit the proliferation of HLC cell line SMMC-7721. The mechanism is correlated with the induction of cell apoptosis by down-regulating the gene expression of bcl-2 gene and up- regulating that of bax gene.

关键词： Annona glabra Linn diterpenes hepatocellular carcinoma apoptosis gene expression

Metabonomic phenotype and identification of “heart blood stasis obstruction pattern” and “qi and yin deficiency pattern” of myocardial ischemia rat models

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Science china(Life Sciences) 2009年第11期52卷 1081-1090页

作者： YAN Bei1, A JiYe1, HAO HaiPing1, WANG GuangJi1, ZHU XuanXuan2, 3, ZHA WeiBin1, LIU LinSheng1, GUAN EnZe3, ZHANG Ying1, GU ShengHua1, HUANG Qing1 & ZHENG YuanTing1 1Key Laboratory of Drug Metabolism and Pharmacokinetics, china Pharmaceutical University, nanjing 210009, china 2Pharmacological Laboratory of Clinical Research Institute, Jiangsu Provincial Hospital of traditional Chinese Medicine, nanjing 2.002., china 3college of Pharmacy, nanjing University of traditional Chinese Medicine, nanjing 2.002., china 1. Key Laboratory of Drug Metabolism and Pharmacokinetics China Pharmaceutical University Nanjing 210009 China 2. Pharmacological Laboratory of Clinical Research Institute Jiangsu Provincial Hospital of Traditional Chinese Medicine Nanjing 210029 China 3. college of Pharmacy Nanjing University of Traditional Chinese Medicine Nanjing 210029 China

The traditional Chinese medicine concepts of "Xinxueyuzuzheng (heart blood stasis obstruction pattern)" and "Qiyinliangxuzheng (qi and yin deficiency pattern)" for myocardial ischemia rat models were constructed in the present study. Endogenous metabolites in rat plasma were analyzed using the GC/TOF-MS-based metabonomic method. Significant metabolic differences were observed between the control and two model groups, and the three groups were distinguished clearly by pattern recognition. Compared with those of the control, the levels of hydroxyproline, threonic acid, glutamine and citric acid were strikingly up or down-regulated in model rats. The metabolites contributing most to the classification between the two "pattern" rats were identified, such as valine, serine, threonine, ornithine, hydroxyproline, lysine, 2-hydroxybutanoic acid, 3-hydroxybutanoic acid, galactofuranose and inositol. These compounds were indicated as the potential biomarkers. The results suggested that the two "patterns" are involved in dysfunction in oxidative stress, energy metabolism and amino acid metabolism. These findings also provided the substantial foundation for exploring the scientific connotation of these two "Zhengxing (pattern types)" of myocardial ischemia, and "Bianzheng (pattern identification)".

关键词： Metabonomics GC/TOF-MS heart blood stasis obstruction pattern qi and yin deficiency pattern myocardial ischemia rat model

Game model of safety monitoring for arch dam deformation

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Science china(Technological Sciences) 2008年第S2期51卷 76-81页

作者： SU HuaiZhi1,2,WU ZhongRu1,2,GU YanChang1,2,HU Jiang1 & WEN ZhiPing3 1 college of Water Conservancy and Hydropower Engineering,Hohai University,nanjing 210098,china 2 National Engineering Research Center of Water Resources Efficient Utilization and Engineering Safety,nanjing 210098,china 3 Department of Computer Engineering,nanjing Institute of Technology,nanjing 210013,china college of Water Conservancy and Hydropower Engineering Hohai University Nanjing 210098 China National Engineering Research Center of Water Resources Efficient Utilization and Engineering Safety Nanjing 210098 China Department of Computer Engineering Nanjing Institute of Technology Nanjing 210013 China

Arch dam deformation is comprehensively affected by water pressure,temperature,dam's structural behavior and material properties as well as other *** them the water pressure and temperature are external factors(source factors) that cause dam deformation,and dam's structural behavior and material properties are the internal factors of deformation(resistance factors).The dam deformation is the result of the mutual game playing between source factors and resistance ***,resistance factors of structure and materials that reflect resistance character of arch dam structure are introduced into the traditional model,where structure factor is embodied by the flexibility coefficient of dam body and the maximum dam height,and material property is embodied by the elastic modulus of *** the basis of analyzing the correlation between dam deformation and resistance factors,the game model of safety monitoring for arch dam deformation is put forward.

关键词： arch dam deformation monitoring model flexibility coefficient game theory

Hybrid method of limit equilibrium and finite element internal force for analysis of arch dam stability against sliding

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Science china(Technological Sciences) 2011年第4期54卷 793-798页

作者： BAO TengFei1,2,3,XU BaoSong1,2,3 & ZHENG XueQing1,2,3 1State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering,Hohai University,nanjing 210098,china 2National Engineering Research Center of Water Resources Efficient Utilization and Engineering Safety,nanjing 210098,china 3college of Water Conservancy and Hydropower Engineering,Hohai University,nanjing 210098,china 1. State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Hohai University Nanjing 210098 China2. National Engineering Research Center of Water Resources Efficient Utilization and Engineering Safety Nanjing 210098 China3. College of Water Conservancy and Hydropower Engineering Hohai University Nanjing 210098 China

A hybrid method of limit equilibrium and finite element internal force for analysis of arch dam stability against sliding is *** finite element internal force method(FEIFM) is used to provide more accurate thrust forces acting on the faces of a slip body,and the limit equilibrium method(LEM) is employed to evaluate the factor of safety of the slip *** method presented can deal with a slip body with large amount of geometrically complex slip *** addition,compared with the traditional LEM,it can meet the balance condition of the forces in the slip *** example shows that the factor of safety obtained by the method presented agrees well with the theoretical solution.A practical example is also presented to demonstrate the application of the method in the stability analysis of an arch dam *** results from the examples show that the method is promising in analysis of arch dam stability against sliding.

关键词： arch dam stability against sliding limit equilibrium method finite element internal force method

APPLYING TCM THEORIES TO IMPROVE THE CURATIVE EFFECTS OF ACUPUNCTURE AND MOXIBUSTION

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针刺研究 1988年第1期 11-16页

作者： Qiu Maoliang nanjing college of traditional Chinese Medicine, china

traditional Chinese Medicine (TCM) has a unique and fairly comprehensivetheoretical system, which has been evolved through constant practice and continual summarization over the past several thousand years,and which hasbeen used to guide the clinical practice of all medical branches includingacupuncture and moxibustion and because of the satisfactory curative effectshave been achieved.

关键词： In APPLYING TCM THEORIES TO IMPROVE THE CURATIVE EFFECTS OF ACUPUNCTURE AND MOXIBUSTION TCM

TRANSMISSION MOMENTARY EFFICIENCY BASED ON THE D'ALEMBERT-LAGRANGE EQUATION FOR INVOLUTES GEARS

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Chinese Journal of Mechanical Engineering 2004年第2期17卷 272-275页

作者： Liang Yi Lai Changying School of Mechanical Engineering,nanjing University of Science and Technology,nanjing 210094, china School of Mechanical Engineering Nanjing University of Science and Technology Nanjing 210094 China Department of Science and Technology Studies University College London WC1E 6BT UK

The D'Alembert-Lagrange equation is introduced and used to derive theformulas of momentary efficiency for external gearing of standard involutes spur gears. The gearingswith correct and increased center distance are discussed. The momentary efficiency formula iscalculated and analyzed using software Matlab. The derived formula of momentary efficiency is alsocompared with the traditional formula.

关键词： D'Alembert-Lagrange equation Involutes gear Momentary efficiency

A restudy of Oryctocephalus indicus (Reed,1910)

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Progress in Natural Science:Materials International 2006年第11期16卷 1177-1182页

作者： ZHAO Yuanlong~1**, YUAN Jinliang~2, PENG Shanchi~ 2,3 , YANG Xinglian~1, PENG Jin~1, LIN Jihpai~4, and GUO Qingjun~5(1. college of Resource and Environment of Guizhou University, Guiyang 550003, china 2. nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences, nanjing 210008, china 3. State Key Laboratory of Papaeobiology and Stratigraphy, nanjing Institute of Geology and Palaeontology, nanjing 210008, china 4. Department of Geological Science, Ohio State University, Columbus, OH 43210, USA 5. Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002, china) college of Resource and Environment of Guizhou University Guiyang 550003 China nanjing Institute of Geology and Palaeontology Chinese Academy of Sciences Nanjing 210008 China State Key Laboratory of Papaeobiology and Stratigraphy Nanjing Institute of Geology and Palaeontology Nanjing 210008 China Department of Geological Science Ohio State University Columbus OH 43210 United States Institute of Geochemistry Chinese Academy of Sciences Guiyang 550002 China

Restudy on morphology of Oryctocephalus indicus (Reed, 1910), based on specimens from Guizhou, china and Nevada USA, suggests that the subspecies regarded previously as Oryctocephalus indicus indicus (Reed, 1910), O. indicus latus Zhao et Yuan 2002, and O. indicus kobayashi Saito, 1934, are synonymous, and Oryctocephalus americanus Sundberg et McCollum, 2003 is a similar form of O. indicus. Oryctocephalus indicus is rediagnosed as having a glabella that is subconical in outline and tapering forward slightly; a thorax that comprises 12 segments, and a small pygidium that bears 2—3 axial rings with a terminal piece and a postaxial ridge. Oryctocephalus indicus is widely distributed in eastern Guizhou and ranges through a great interval of Kaili Formation. Its first appearance is almost identical with these events of trilobite extinction-recovery occurring at the end of the Early Cambrian, the alternation of acritach assemblages, the change of trace elements (REE) and stable isotopes (carbon) at the Wuliu-Zengjiaya section. As one of the key forms for defining the traditional Lower-Middle Cambrian boundary (the base of Cambrian Series 3 and Stage 5 in the new Cambrian chronstratigraphic standard), O. indicus has received detailed study and has more advantages than other species such as Ovatoryctocara granulata Tchernysheva, 1962 or Arthricocephalus chauveaui Bergeron, 1899.

关键词： Oryctocephalus indicus restudy Kaili Formation Cambrian Guizhou china.

ANALYSIS OF HUMAN PLACENTA BY ^(31)P NUCLEAR MAGNETIC RESONANCE AND THIN-LAYER CHROMATOGRAPHY SCANNING COMBINED WITH THE CORRECTIVE METHOD OF ABSORBANCE PROPORTIONAL COEFFICIENT

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Chinese Chemical Letters 1993年第12期4卷 1081-1084页

作者： Rong GUO Jian wei CHEN Yi Min XU AiHua ZHANG nanjing college of traditional Chinese Medicine,nanjing 2.002.

Five phospholipids in human placenta were determined by phosphorus 31 nuclear magnetic resonance(^(31)P NMR)spectroscopy and thin-layer chromatography(TLC) scanning combined with the corrective method of absorbance proportional coefficient. The NMR spectrometer used this investigation was a Bruker AM-500 spectrometer operating at 202.4 MHz for ^(31)P chemical shifts are relative to 85% phosphoric acid. TIC was carried out by silica gel H plate developed in chloroform-methanol-glacial acetic acid-ethanol-water(25:4:6:2:0.5),with Vaskovsky reagent as colour -developing agent of phospholipids.

关键词： P NUCLEAR MAGNETIC RESONANCE AND THIN-LAYER CHROMATOGRAPHY SCANNING COMBINED WITH THE CORRECTIVE METHOD OF ABSORBANCE PROPORTIONAL COEFFICIENT ANALYSIS OF HUMAN PLACENTA BY LPC TLC

Solutions and solvabilities of general binary quadric indefinite equations

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Chinese Science Bulletin 1997年第7期42卷 541-545页

作者： LiU LiAnhui traditional Chinese Medical college, Hefei 230038, china Anhui traditional Chinese Medical college Hefei China

To this day for special m and small |c|, solvable, c are obtained about general equations x^2-my^2=c with positive squarefree integer m and integer c by Ankeny, Mollin et al. Afterwards, Zhang in ref. [1] greatly developed their correspondent results and gave all proper integer solutions and solvable c about the equations for general m. Then Lu in ref. [2] gave results for small |q| about general binary quadric indefinite

关键词： general binary quadric indefinite equation proper integer solution solvability.