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Cesium accumulation by bacterium Thermus sp. TibetanG7:hints for biomineralization of cesium-bearing geyserite in hot springs in Tibet,china

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Chinese Science Bulletin 2007年第19期52卷 2680-2686页

作者： WANG HaiLei1,2,3, ZHENG MianPing1,2,3 & HUANG XiaoXing4 1 Institute of Mineral Resources, Chinese Academy of Geological Sciences (cags), beijing 10003., china 2 R&D Center for saline lake and Epithermal Deposits, cags, beijing 10003., china 3.open laboratory of saline lake resourses and environment, cags, beijing 10003., china 4 laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, beijing 100081, china Institute of Mineral Resources Chinese Academy of Geological Sciences (CAGS) Beijing China R&D Center for saline lake and Epithermal Deposits CAGS Beijing China open laboratory of saline lake resourses and environment CAGS Beijing China laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing China

The bacterium Thermus sp. TibetanG7, isolated from hot springs in Tibet, china, was examined for the ability to accumulate cesium from solutions. environmental conditions were simulated and the effects of pH, K+, Na+ and K+-regimes were then studied to determine the possible role of the bacterium in the formation of cesium-bearing geyserite around these hot springs. In despite of the inhibition of K+ and Na+, the bacterium Thermus sp. TibetanG7 revealed noticeable accumulation of cesium from solutions, with maximum accumulations of 53.49 and 40.41 μmol Cesium/g cell dry weight in Na+ and K+ inhibition experiments, respectively. The accumulation of cesium by this microorganism is rapid, with 40%―50% accumulated within the first 5 min. K+-deficient cells showed a much higher capacity of cesium accu- mulation compared with K+-sufficient cells. It is evident that the bacteria within the genus thermus play a significant role in the cesium assembly. The formation of cesium-bearing geyserite is also considered.

关键词：铯元素沉积物硅华生物矿化

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The effects of K^+ growth conditions on the accumulation of cesium by the bacterium Thermus sp. TibetanG6

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Science china(Life Sciences) 2006年第2期49卷 123-129页

作者： WANG Hailei1,2,3, KONG Fanjing1,2,3 & ZHENG Mianping1,2,3 1. R&D Center for saline lake and Epithermal Deposits, Chinese Academy of Geological Sciences, beijing 10003., china 2. Key open laboratory of saline lake Resources and environment, Chinese Academy of Geological Sciences, beijing 10003., china 3. Institute of Mineral Resources, Chinese Academy of Geological Sciences, beijing 10003., china R&D Center for saline lake and Epithermal Deposits Chinese Academy of Geological Sciences Beijing China Key open laboratory of saline lake Resources and environment Chinese Academy of Geological Sciences Beijing China Institute of Mineral Resources Chinese Academy of Geological Sciences Beijing China

The accumulation of cesium by the bacterium Thermus sp. TibetanG6 was examined under different K+ growth conditions. The effects of external pH and Na+ on the accumulation of ce-sium were also studied, and the mechanism involved was discussed. K+ regimes played an important role in the accumulation of cesium by the strain TibetanG6. The quantity of cesium accumulated (24 h) was much higher in K+-deficient regime than that in K+-sufficient regime. The pH and Na+ had different effects on the accumulation of cesium in the two K+ regimes. IR spectra analyses indicated that the biosorption is a process of homeostasis with cesium initially accumulated on the cell wall.

关键词： Thermus sp. TibetanG6, cesium, bioaccumulation, K+-growth condition, pH, sodium, IR spectroscopy.

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Carbon and Oxygen Stable Isotope Values and Microfossils at 41.4-4.5ka BP in Tai Co,Tibet,china,and Their Paleoclimatic Significance

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Acta Geologica Sinica(English Edition) 2011年第5期85卷 1036-1050+1052-1056+1051页

作者： ZHENG Mianping~(1,2,*),LIU Junying~(1,3),MA Zhibang~(1,4),WANG Hailei~(1,2) and MA Nina~(1,2) 1 MLR Key laboratory of saline lake Resources and environment,Institute of Mineral Resources,cags, beijing 10003.,china 2 Research and Development Center for saline lakes and Epithermal Deposits,Chinese Academy of Geological Sciences,beijing 10003.,china 3 Institute of Geology,Chinese Academy of Geological Sciences,beijing 10003.,china 4 Key laboratory of Cenozoic Geology and environment,Chinese Academy of Sciences,beijing 100029, china MLR Key laboratory of saline lake Resources and environment Institute of Mineral Resources CAGS Beijing 100037 China Research and Development Center for saline lakes and Epithermal Deposits Chinese Academy of Geological Sciences Beijing 100037 China Institute of Geology Chinese Academy of Geological Sciences Beijing 100037 China Key laboratory of Cenozoic Geology and environment Chinese Academy of Sciences Beijing 100029 China

Two sedimentary sections were measured at Tai Co(Co means lake in Tibetan) in western Tibet,*** two sections are almost all composed of clay carbonate beds except in their lower parts where there are carbonized plants at>10 cm depths and dark-colored carbonate clay and clay at 50-70 cm depths,yielding abundant gastropods,ostracods,and *** carbon and oxygen stable isotope values of carbonate,ostracods,and charophytes and ecological features of microfossil communities indicate the following climatic change in the area from 41.4 to 4.5 ka BP:at 41.4-26.2 ka BP,the climate was relatively wet;at 26.2-25.5 ka BP,it was slightly warm-dry;at 25.5-22.5 ka BP,it was warm-wet;at 22.5-21.0 ka BP,it was slightly cold-wet;at 20.5-17.5 ka BP,it became cold abruptly and slightly wet,implying the climate of the last glacial maximum;at 17.5-16.0 ka BP,it was slightly cold-dry;at 16.0-11.8 ka BP,it was slightly warm-wet;at 11.8-10.4 ka BP,it was relatively cold-dry, roughly equivalent to the climate of the Younger Dryas,and at 10.4 ka BP,the temperatures began to rise again;at 10.4-9.4 ka BP,it was slightly warm-wet;at 9.4-8.5 ka BP,there occurred short warm-wet oscillations;at 8.5-7.9 ka BP,it was slightly dry-cold,representing a strong temperature-lowering and drying event in the postglacial stage;at 7.8-6.3 ka BP,it was slightly warm-wet;at 6.3-4.5 ka BP,the climate tended to be cold-dry.4.5 ka BP recorded the maximum aridity since the late part of the late Pleistocene.

关键词： sedimentary environment index paleoclimatic changes late Pleistocene-Holocene(41.4-4.5 ka BP) Tai Co Tibet

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Cloning and Characterization of a Family of Disease Resistance Gene Analogs from 6VS of Haynaldia villosa

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Agricultural Sciences in china 2003年第8期2卷 937-942页

作者： KONG Fan-jing, MA You-zhi, CHEN Xiao and XIN Zhi-yong(Institute of Crop Breeding and Cultivation , Chinese Academy of Agricultural Sciences , beijing 100081,P. R. china open laboratory of saline lake Resources and environment of Ministryof Land and Resources , beijing 10003. , P.R. china) Institute of Crop Breeding and Cultivation Chinese Academy of Agricultural Sciences Beijing 100081P.R. China open laboratory of saline lake Resources and environment of Ministry of Land and Resources Beijing 100037 P.R. China Institute of Crop Breeding and Cultivation Chinese Academy of Agricultural Sciences Beijing 100081P.R. China

In the present study, microdissection of 6VS and the cloning of the resistance gene analogs(RGA)from them were reported. The 6VS were microdissected with needle and 10 types of resistance gene analogs were obtained by PCR with degenerate oligonucleotide primer designed according to resistance genes. They were designated as Hvrgak1-Hvrgak10, GenBank accession numbers are AF387113-AF387121, AY040671- AY040672. Identity among RGAs was about 10-50%, and identity with cloned R gene from plants was 5-20%. Southern hybridization analysis results showed 3 RGAs, Hvrgak2, Hvrgak4, and Hvr-gak5 were linked with wheat powdery mildew resistance. These RGAs may be used as direct entrance or probes for cloning the disease resistance genes.

关键词： 6VS of Haynaldia Villosa Microdissection Resistance gene analogs(RGA) Cloning

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Preliminary discussion of low-salinity hydrothermal fluid mineralization

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Chinese Science Bulletin 1999年第S2期44卷 141-143页

作者： ZHENG Mianping R & D Center of saline lake and Epithermal Deposits, Chinese Academy of Geological Sciences, beijing 10003., china R and D Center of saline lake and Epithermal Deposits Chinese Academy of Geological Sciences Beijing 100037 China

REVIEWING the history of the geology of mineral deposits, we may find that in the first fifty years of thetwentieth century the magmatic-hydrothermal theory of ore deposition was the most prevalent, whichgave impetus to the development of the geology of mineral deposits. In the 1960s, the drilling of theSalton Sea geothermal field, California, the United States, and discovery and study of the Red Sea seafloor hot brines and large amounts of metal sulfides removed therefrom led to the rise of the brine theory ofmineral deposition, thus elucidating the origin of massive metal sulfides and promoted the discoveryand exploration of metal deposits. After the 1970s and the 1980s, through a study of the hydrothermal ore fluids of modern hotsprings. geologists came to realize that there exist low-salinity hydrothermal ore fluids other than

关键词： low-salinity hydrothermal fluid mineralization.

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Evidence of the Pan-lake Stage in the Period of 40-28 ka B.P. on the Qinghai-Tibet Plateau

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Acta Geologica Sinica(English Edition) 2000年第2期74卷 266-272页

作者： ZHENG Mianping, MENG Yifeng and Wei LejunResearch & Development Center of saline lake and Epithermal Deposits,Chinese Academy of Geological Sciences, beijing 10003. Chinese Acad Geol Sci Res & Dev Ctr Saline Lake & Epithermal Deposits Beijing 100037 Peoples R China

The Qinghai-Tibet plateau is one of major saline lake regions in china, where saline lakes are widespread and constitute an important object of researches on the palaeoclimatic change in the region. On the basis of comprehensive investigations of the evolution of the lake's surface and sediments on the plateau, the authors have further demonstrated the existence of a pan-lake stage (river and lake flooding stage) on the Qinghai-Tibet plateau during the period of about 40+-28 ka B.P. and analyzed the palaeoclimatic characteristics of the pan-lake period and relationships between the ancient monsoons and the uplift of the plateau since the beginning of the Quaternary.

关键词： Qinghai-Tibet plateau Late Pleistocene pan-lake event palaeoclimate

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Primary Study on Quantitative Reconstruction of Middle-Late Eocene Climate in Jianghan Basin

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Journal of china University of Geosciences 2002年第3期13卷 252-259页

This paper reports our primary effort in the quantitative reconstruction ofpaleoclimate based on the thrine in phytoecology of the affinity parent plants in the stratigraphicpollen records. The Eocene pollen data come from our former study on the Mingjia borehole 1 in theJianghan basin. The fluctuating trend in the parameter curve of climate shows that the climate inthe Middle Eocene in the Jianghan basin was more or less comparable with that of the present22°―26°N, characteristic of a humid, semi-humid central-southern subtropical climate. The annualtemperature at that time dropped by 1 ℃― 4 ℃ in the Late Eocene, approximately equal to that ofthe present 23°―28°N of northern-central subtropical climate. However, the climate composite atthat time, characterized by higher temperature, small annual range and big fluctuation inprecipitation, was quite different from the present one. The average temperature in January in theMiddle Eocene, higher than that of today, ranged between 5 ℃ and 9 ℃, indicating that no effect ofwinter monsoon occurred in the Middle Eocene, though such an effect may have occurred occasionallyin the Late Eocene. Major temperature decline is recognized at the depth of 2 100 m in the borehole,as was indicated by the decline in average January temperature, the increase in annual range, andthe increase in the deciduous broad-leaved types of trees in the spore-pollen assemblage. The sharpfluctuation in the annual precipitation, usually raging from 300 to 1 700 mm, was favorable for themigration and accumulation of salty deposit. When the precipitation was lower than 1 000 mm,ephemera shrub increased at the same depth as that of the salty deposit. It is, therefore, deducedthat the formation of the salty deposit was attributed mainly to the dry and hot environment in thehigh mountains and deep basins. The small annual precipitation and the intense fluctuation arefavorable for the sustainable accumulation of the salts, which is different from the present

关键词： quantitative reconstruction of paleoclimate annual range function ofthrine in phytoecology fuzzy mathematics eocene jianghan basin

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HOLOCENE saline SEDIMENTARY RECORD IN TIBETAN DONG CO(lake) AND ITS SIGNIFICANCE ON PALAEOCLIMATE

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地学前缘 2000年第S1期7卷 378-378页

作者： Wei Lejun 1,2 ,Zheng Mianping 2,3 [WT5,5”BX](1 china University of Geosciences,beijing 100083,china 2.open laboratory of saline lake Resources & environment ,Chinese Academy of Geological Sciences,beijing 10003.,china 3.Institute of Min

Based on the sedimentary characteristics study of Holocene borax\|bearing mirabilite beds of Dong Co(lake) two sections, DZ01 and DZ02, in the center of North\|Tibetan Plateau,combined with dating of 14 C, applied theory and method of comparative salinology, it is determined that the palaeoclimate framework of Dong Co during early\|middle Holocene, and reckoned the range of the temperature variation. Furthermore, after completing regional analogue, discovered that the palaeoclimate environment is fully comparative and consistent with the synchronous lake\|water retreating trace of Zhaduixiong spits in Zabuye lake basin, with palaeoclimate changes recorded by pollen in adjacent Zabuye lake, with Holocene plaeoclimate environment indicated by drilling\|hole in Bangong Co,with palaeoclimate significance traced by δ 18 O value in Qinghai lake and Siling Co and with the palaeoclimate framework during the 5th Level Arid (Salinization) Extension Period of Quaternary arid center of Western china.

关键词： Dong Co Tibet Holocene sedimentary record palaeoclimate change s

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The Formation of the Qarhan saline lakes as Viewed in the Light of Neotectonic Movement

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Acta Geologica Sinica(English Edition) 1990年第3期64卷 247-259页

作者： Zhu Yunchu Li Zhengyan Wu Bihao Wang Mili Research Institute of Exploration and Development of saline lakes, Golmud, Qinghai Institute of Mineral Deposits, cags Jia Zhongpeng 1Li Songyun Chen Yongzhi Liu Chenglin and Li Wensheng also took part in the work.

Investigations on stratigraphy, geomorphology and neotectonic movement in the Eastern Kunlun Moun-tains show that there existed a series of ancient lakes, including some saline lakes. in the studied region about30,000 years ago, They were distributed south of the middle Kunlun fault. from the middle-upper reaches of thepresent Narin Gol River in the west to the Alag lake-Tosou lake in the east. Of these the ancient Narin Gollake and Kunlun lake were mainly recharged by the hot water related to valcanos. so the B, Li and K con-tents are relatively high. The neotectonic movement that commenced at 30.000 a B.P. caused the river system in the EasternKunlun Mountains to invade southwards. as a result the ancient lake water was captured to recharge theQarhan area. Therefore, the hot springs related to recent volcanism and faulting on the southern bank of theupper reaches of the Narin Gol River became an important source of saline materials for the Qarhan lake.

关键词： The Formation of the Qarhan saline lakes as Viewed in the Light of Neotectonic Movement lake

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Palaeoclihlatic Indicators of china's Quaternary saline lake Sediments and Hydrochemistry

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Acta Geologica Sinica(English Edition) 2000年第2期74卷 259-265页

作者： ZHENG Mianping, ZHAO Yuanyi and LIU JunyingResearch & Development Center of saline lake and Epithermal Deposits,Chinese Academy of Geological Sciences, beijing 10003.

In this paper the authors classify saline lake sediments into the cold, warm and eurythermal phases, reveal the consistency between the zoning of hydrochemical types of modern saline lake water and climatic zoning and give climatic parameters under the conditions of typical cold phase (mirabilite and natron), warm phase (thenar-dite) and slightly warm phase (bloedite) saline lake deposition.

关键词： saline lake sediments hydrochemistry palaeoclimatic indicator Quaternary

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