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Science China(Life Sciences) 2010年第6期53卷 690-700页

作者： LI Lin1,LI Hui1,LI JiYing1,XU ShuTu1,YANG XiaoHong1,LI JianSheng1 & YAN JianBing1,2 1National maize improvement Centre of China,China Agricultural University,Beijing 100193,China 2international maize and wheat improvement Centre (CIMMYT),Apdo.Postal 6-641,06600 Mexico,D.F.,Mexico 1. National maize improvement Centre of China China Agricultural University Beijing 100193 China2. International Maize and Wheat Improvement Centre (CIMMYT) Apdo. Postal 6-641 06600 Mexico D.F. Mexico

Lipids play an important role in plants due to their abundance and their extensive participation in many metabolic *** involved in lipid metabolism have been extensively studied in Arabidopsis and other plant *** this study,a total of 1003 maize lipid-related genes were cloned and annotated,including 42 genes with experimental validation,732 genes with full-length cDNA and protein sequences in public databases and 229 newly cloned ***-seven maize lipid-related genes with tissue-preferential expression were discovered by in silico gene expression profiling based on 1984483 maize Expressed Sequence Tags collected from 182 cDNA ***,70 QTL clusters for maize kernel oil were identified,covering 34.5% of the maize ***-nine (84%) QTL clusters co-located with at least one lipid-related gene,and the total number of these genes amounted to ***,thirteen genes with kernel-preferential expression profiles fell within QTL clusters for maize kernel oil *** the maize lipid-related genes identified here may provide good targets for maize kernel oil QTL cloning and thus help us to better understand the molecular mechanism of maize kernel oil accumulation.

关键词： lipid metabolism in silico gene cloning in silico gene expression profiling quantative trait locus(QTL) maize

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Cloning and characterization of a novel R1-MYB transcription factor in maize

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Progress in Natural Science:Materials international 2009年第9期19卷 1089-1096页

作者： Guanqing Jia, Bo Li, Dengfeng Zhang, Tifu Zhang, Zhiyong Li, Jingrui Dai, Shoucai Wang* National maize improvement center of China, Key Laboratory of Crop Genomics and Genetic improvement of Agriculture Ministry, Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China [a]National maize improvement center of China Key Laboratory of Crop Genomics and Genetic Improvement of Agriculture Ministry Department of Plant Genetics & Breeding China Agricultural University Beijing 100193 China

R1-MYB transcription regulatory factors play vital roles in transcriptional modification during higher plant metabolism and development. In the present study, an R1-MYB gene was isolated from the maize (Zea mays L.) inbred line C8605-2 based on expressed sequence tags (ESTs), which expressed differentially between the hybrid C8605-2 × W1445 and its two parents in an oligonucleotide microarray. The full-length cDNA, designated as ZmMYBE1 (GeneBank Accession No. FJ024049), consists of 1992 nucleotides and contains a 1296-bp open reading frame. One conserved MYB domain was detected near the N-terminus of the deduced amino acid sequence, where an acidic Ser/Thr-rich area was also observed in the downstream region. The sequence of ZmMYBE1, mapped to chromosome 5 (bin 5.03), was revealed belonging to a multi-gene family in the maize genome. Subcellular localization analysis determined that ZmMYBE1 was located in the nucleus. Expression analysis showed that ZmMYBE1 transcripts accumulated in various tissues examined, with high expression levels in the immature ear and low levels in the tassel, root, and stem. The expression analysis for ZmMYBE1 in immature ears showed the highest expression level at the earliest vegetative developmental stage of the plant. Expression of ZmMYBE1 higher than that of the parents was observed in the hybrid C8605-2 × W1445. And also the expression level similar to the lowest of the parents in the hybrid C8605-2 × W245 was observed, revealing different expression patterns for the same gene in various hybrids combined from diversified inbred lines. Transgenic Arabidopsis overexpressing ZmMYBE1 implies that ZmMYBE1 has an important role in developmental regulation in maize.

关键词： MYB transcription factor Differential expression maize

Molecular mapping of leaf rust resistance gene LrBi16 in Chinese wheat cultivar Bimai 16

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Molecular mapping of leaf rust resistance gene LrBi16 in Chi...

2010中国作物学会学术年会

作者： Zhou Yue~1,Zhang Hai~1,Xia Xianchun~2,He Zhonghu~(2,3),Li Xing~1,Li Zaifeng~(1*),Liu Daqun~(1*) (1 College of Plant Protection,Agricultural University of Hebei/ Biological Control center for Plant Diseases and Plant Pests of Hebei Province,Baoding 071001,China 2 Institute of Crop Science,National wheat improvement center/The National Key Facility for Crop Gene Resources and Genetic improvement,Chinese Academy of Agricultural Sciences (CAAS),Beijing 100081,China 3.international maize and wheat improvement center(CIMMYT) China Office,c/o CAAS,Beijing 100081,China)

Leaf rust,caused by Puccinia triticina,is one of the most widespread diseases in common wheat (Triticum aestivum L.) *** the objective of identifying and mapping new genes for resistance to leaf rust,F,F plants and F lines from a cross between resistant cultivar Bimai 16 and susceptible cultivar Thatcher were inoculated with Chinese Puccinia triticina pathotypes FHTT and PHTS in the *** the first seedling test,Bimai 16,Thatcher,20 F plants,359 F plants and 298 F lines were inoculated with pathotype FHTT.A set of 1,255 SSR primers pairs were used to test the parents,

关键词： Common wheat Triticum aestivum SSR marker Puccinia triticina brown rust

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选育优质蛋白玉米种质的最近进展

作物学报 1983年第3期 165-172页

作者：邓澄欣墨西哥国际玉米小麦改良中心玉米研究组

本文简述选育优质蛋白玉米所遇到的问题及其解决途径。国际玉米小麦改良中心(以下简称“改良中心”)过去十多年来采用奥帕克2基因及其修饰基因的作用,将原来的粉质籽粒逐渐改成硬胚乳籽粒,同时保存了高营养品质,使籽粒中的赖氨酸和色氨... 详细信息

本文简述选育优质蛋白玉米所遇到的问题及其解决途径。国际玉米小麦改良中心(以下简称“改良中心”)过去十多年来采用奥帕克2基因及其修饰基因的作用,将原来的粉质籽粒逐渐改成硬胚乳籽粒,同时保存了高营养品质,使籽粒中的赖氨酸和色氨酸含量仍然高过普通玉米,在育种上取得了显著进展。整个育种过程可分为三个阶段:(1)选获优质蛋白玉米的供体原种,(2)选育优质蛋白玉米种质,(3)管理种质的新方法。本文详述了这些阶段的工作目标以及采用的育种方法。“改良中心”现已成功地培育了大批优良的优质蛋白玉米种质,克服了奥帕克2玉米的各种缺点。目前拥有的优质蛋白玉米种质包括13个基因库材料和10个高代群体,分别属于热带和亚热带材料,并以籽粒的色泽和质地加以分类。依靠新的管理方法和国际多点后裔试验,这些优质蛋白玉米的种质将得到进一步的改良和广泛利用。

关键词：优质蛋白玉米普通玉米供体原种种质籽粒

Genetic analysis of segregation distortion of mating-type factors in Lentinula edodes

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Progress in Natural Science:Materials international 2005年第8期15卷 684-688页

作者： CHENG Shuiming 1,2, LIN Fanxue1, XU Xuefeng1, LI Anzheng1 and LIN Fangcan1*(1. State Key Laboratory of Agricultural Microbiology and Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070, China 2. National Engineering Research center For wheat, Henan Agricultural University, Zhengzhou 450002, China) State Key Laboratory of Agricultural Microbiology and Institute of Applied Mycology Huazhong Agricultural University Wuhan 430070 China. National Engineering Research center For wheat Henan Agricultural University Zhengzhou 450002 China

Analyses of the mating-type ratio of protoplast monokaryons to spores in 28 strains of Lentinula edodes were performed through statistical methods. The results showed that the segregation distortion phenomenon commonly exists in both types of Lentinula edodes, 17 out of the 28 samples did not display the expected 1∶1 ratio of protoplast monokaryons; and among them 8 fruiting strains distorted 1∶1∶1∶1 segregation of spores. The degree of distortion in all tested wild strains was significantly lower than that of cultured strains. In fruiting test of positive-negative parent, predominant spores were those with the mating type identical to dikaryotic parent, indicating that the predominance of nuclei mainly depends on the mating type of dikaryotic parent. Data from di-mon mating test verified that monokaryons with acceptor nuclear type are present exclusively in a large amount after dedikaryotization, suggesting that the specificities among B factors and some other unknown factors in cytoplasm are probably responsible for the phenomenon of skewed segregation.

关键词： Lentinula edodes mating-type factors segregation distortion cytoplasm.

Cloning and characterization of an up-regulated GA 20-oxidase gene in hybrid maize

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Progress in Natural Science:Materials international 2009年第2期19卷 161-166页

作者： Jinkun Du,Yingyin Yao,Zhongfu Ni,Qixin Sun Department of Plant Genetics & Breeding and State Key Laboratory for Agrobiotechnology,Key Laboratory of Crop Genomics and Genetic improvement,Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic improvement,China Agricultural University,Beijing 100094,China [a]Department of Plant Genetics & Breeding and State Key Laboratory for Agrobiotechnology Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100094 China

Previous studies revealed that GA content and metabolism are positively correlated with a faster shoot growth rate of hybrid,and recently,genes participating in both GA biosynthesis and GA response pathways were also found to be differentially expressed between wheat hybrid and its parental inbreds. In this study,an up-regulated GA 20-oxidase gene in a maize hybrid,designated ZmGA20,was cloned. ZmGA20 contains an open reading frame (ORF) encoding 391 amino acid residues. BLASTX searches in GenBank revealed that the ZmGA20 is homologous to the sequences of GA20ox proteins from different species,and analysis also indicated that ZmGA20 had typical features of GA 20-oxidase proteins,including a "LPWKET" sequence. Semi-quantitative RT-PCR analysis showed that ZmGA20 was expressed in different tissues and/or organs. The expression level of ZmGA20 in the hybrid was higher than that in two parents (in roots,leaves,stems and embryo,and ears). The abundance of ZmGA20 transcript was equal to that of the highly expressed parents,which provided molecular evidence for the observed GA content heterosis in maize hybrids.

关键词： GA 20-oxidase Gene expression maize Heterosis

Future perspective of China's feed demand and supply during its fast transition period of food consumption

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Journal of Integrative Agriculture 2015年第6期14卷 1092-1100页

作者： DONG Wan-lu WANG Xiao-bing YANG Jun center for Chinese Agricultural Policy Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences University of Chinese Academy of Sciences School of international Trade and Economics University of International Business and Economics

China has experienced dramatic changes in food consumption patterns over the last three decades. However, there are different opinions regarding the future trends in consumption. By adopting the well-developed partial equilibrium model-China Agricultural Policy Simulation Model（CAPSi M）, the demand for livestock products and the main feed crops over 2011–2030 is predicted and analyzed. It is found that China＇s per capita consumption of livestock products will continue to rise during this period, even though its growth rate will slow down gradually. Meanwhile, the expansion of livestock production will pose great challenges for feed supply in China. More accurately, China will be confronted with feed security rather than grain security in the future.

关键词： livestock experienced dramatic thousand capita challenges urbanization soybean agricultural imports

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Genetic Diversity of Local maize Varieties from Southwestern China

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Genetic Diversity of Local Maize Varieties from Southwestern...

Air-Soil Exchange of Endosulfan in China

玉米遗传育种国际学术讨论会

作者： H.Lu J.S.Li J.L.Liu(1Department of Agronomy Purdue University West Lafayette IN47907-1150 USA2Current address: maize improvement center of China China Agricultural University Beijing China 100094 ’Department of Agronomy Huazhong Agricultural University Wuhan China 430070)

＜正＞Local maize grmalasm is an important resources for maize hybrid breeding in China. Therewere abundant local maize varieties in southwestern China. Our objectives were to evaluste the allozyme poly-morphism of these local varieties and to group them based on allozyme data. Twenty-seven local maize varietiesfrom southwestern China and three U. S. populations were analyzed for allozym variation at 18 allozyme loci.A total of 69 alleles at 18 isozyme loci were datected with an average of 3.83 alleles per locus. Compared withvarious maize germplasm from U. S. Corn Belt, twenty-seven Chinese local varieties had a signifinantly highernumber of allozyme alleles. Local maize varieties from southwestern China might be a valuable gene pool withan abundant genetic diversity. But three U. S. populations （Lancaster, BS13 （S） C 2, and Pingzong） inthis study each had a higher expected heterozygosity than the average heterosigosity of all studied materials. The71.1% of the allozyme variation resided within varieties, and 28.9% among them, indicating Chinee localmaize varieties differentiated each other very well. cluster analysis classified twenty-seven local varieties andthree U. S. pepulations into four major groups. This might be useful for classifying local varieties into differentheterotic groups.

关键词： maize Local variety Allozyme Genetic variation Southwestern China

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Air-Soil Exchange of Endosulfan in China

On the determination of nitrous oxide emission factor during biomass burning

持久性有机污染物论坛2011暨第六届持久性有机污染物全国学术研讨会

作者： Hongliang Jia~1,Zhi Zhang~2,Meng Yang~1,Yi-Fan Li~(3,1,2*) (1 international Joint Research center for Persistent Toxic Substances(IJRC-PTS),Dalian Maritime University,Dalian,P.R.China 2 IJRC-PTS,State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,P.R.China 3 Science and Technology Branch, Environment Canada,Toronto,Canada

*** Endosulfan has become a global challenge,due to its long-term usage and its properties of persistent organic pollutants(POPs).Endosulfan has been applied in China to control pests in cotton since 1994,and in wheat,tea,tobacco,apple and other fruits since *** total usage of endosulfan in China was estimated to be approximately 25,700 t between 1994 and *** first Chinese gridded inventories of usage,emissions to air,and residues in soil inventories with a 1/4°longitude by 1/6°latitude resolution forα-,β-endosulfan and endosulfan sulfate were produced for ***

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Journal of Environmental Sciences 1994年第3期6卷 261-267页

作者： Cao Meiqiu Zhuang Yahui (Research center for Eco-Environmental Sciences, Chinese Academy of Sciences) P. O. Box 2871,Beijing 100085,China

The emission factors of nitrous oxide have been determined during the combustion of rice straws, maize stalks and wheat stalks in an enclosed combustion system. They equal to 84.4 ± 6.08g/t for rice straws,132± 8.63g/t for maize stalks,and 27.3 ±1.79g/tfor wheat stalks,respectively. The uncertainties in the determination of nitrous oxide have been discussed. The N_2O-N(nitrogen in nitrous oxide emission)accounts for 0. 59% and 0. 87% of the total nitrogen in rice straws and maize stalks,respectively. An 1￣0 ×1￣0 grid map on the distribution of N_2O emission from biomass burning in China mainland was shown.

关键词： biomass burning,nitrous oxide emission factor

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