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Molecular Cloning,Tissue Distribution and Ontogenetic Expression of the Amino Acid Transporter b0,+cDNA in the small intestine of Tibetan suckling piglets

Molecular Cloning,Tissue Distribution and Ontogenetic Expres...

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4th International Symposium on Animal Nutrition,Health and Feed Additive

作者： W.C.Wang,~(1,4) W.T.Gu,~(1,4) X.F.Tang~2,M.M.Geng,~1 M.Fan~3.T.J.Li,~1 W.Y.Chu~(1,5*) Changyou Shi,~1 R.L.Huang,~1 H.F.Zhang~2,Y.L.Yin~(1*) 1 Key Laboratory for Agro-ecological Processes in subtropical Region,institute of subtropical {3.,The chinese Academy of sciences,changsha,Hunan 410125,P.R.china 2 china National Key Laboratory of Animal Nutrition,Beijing Animal and Veterinary Science institute,chinese Agricultural Academy,Beijing china,100094 3.Department of Animal and Poultry Science,University of Guelph,Guelph,Ontario N1G 2W15, Canada . 5 Department of Bioengineering and Environmental Science,changsha University,changsha, Hunan,410003.P.R.china

Tibetan pig has been given more attention recent years for its ability to survive under adverse *** it is an ideal small experimental animal,for its small body size, extremely similarity with human in anatomy and physiology,*** small intestine is the main absorption place of peptides and free amino acids,which required many transporter *** b mediates apical uptake of basic amino acids,especially lystine,argine and *** aim of current study was to clone Tibetan porcine amino acid transporter bAT（SLC7A9） for comparing the sequences of tibetan and common pig,and investigating the tissue distribution and ontogenetic expression in the small intestine of Tibetan suckling *** Tibetan porcine SLC7A9 gene which cloned from the porcine small intestine was first described, encoding the amino acid transporter bAT. The entire open reading frame（ORF） of the SLC7A9 is 1464 bp and codified 487 amino acid residues,having a higher degree of sequence similarity with the horse counterpart （91.2%） than with monkey（89.5%） or human （88.7%）.The putative protein has 12 putative transmembrane *** this study, SLC7A9 mRNA was detected in brain,kidney, duodenum,jejunum,ileum,heart,liver,lung and muscle from Tibet pigs at 7 and 21 days by ***,we investigated the expression of Tibetan sucking piglets in duodenum, anterior jejunum,posterior jejunum,ileum and kidney from 1 to *** abundance of SLC7A9 mRNA in duodenum and jejunum was highest and lowest,*** has a similar pattern in duodenum and anterior jejunum,which the mRNA level was decreased in front of the sucking period and increased until *** jejunum expression was moving up steadily with age, except *** ileum has the highest expression at d14 and becomes steady after *** mRNA abundance in kidney has the trend in opposite to duodenum,raised until 14 and reduced after *** results showed the pattern of bAT expressed in small intestine of Tibetan pig and thi

关键词： amino acid transporter b0,+ AT Tibetan piglet tissue expression

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Effects of Lactosucrose on Intestinal Barrier in TNBS-induced Colitis in Rats

Effects of Lactosucrose on Intestinal Barrier in TNBS-induce...

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中国畜牧兽医学会动物营养学分会第十一次全国动物营养学术研讨会

作者： Z.Ruan~1 X.Huang~1 X.Wu~(1,2) Y.Zhou~1 G.WU~3.Y.Yin~(1,2*) (1.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 3.0047,china 2.Research Center for Healthy Breeding of Livestock and Poultry,Hunan Engineering and Research Center of Animal and Poultry Science,institute of subtropical {3.,the chinese Academy of sciences, changsha 410125,china 3.College of {3. and Life sciences,Texas A&M University,College Station 77843.2471,USA)

Aims To investigate the effects of Lactosucrose on intestinal barrier in TNBS-Induced *** Lactosucrose were administered rats with experimental ulcerative colitis induced by 2,4,6-trinitrobenzene sulfonic acid(TNBS).The colon structure was observed hematoxylin-eosin ***,a kind of tight junction protein,were determined by immunohistochemical *** mRNA expression was assessed by reverse transcriptase-polymerase chain reaction(RT-PCR).Results Lactosucrose significantly improved changes in the histological inflammation score as compared with TNBS-induced *** protein content of occludin and its mRNA expression were increased compared with TNBS-induced *** These results suggest that Lactosucrose can recover mucosal injury due to increase of occludin in TNBS-induced colitis.

关键词： Lactosucrose Intestinal barrier Oceludin Colitis

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FZP promotes the MADS box genes involved in specifying floral identity and suppresses RFL and LAX1 to regulate panicle branching in rice

FZP promotes the MADS box genes involved in specifying flora...

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第十三届全国植物基因组学大会

作者： Xufeng Bai~1,Donghai Mao~(1,2),Song Song~1,Mi Wen~1,Li Zhang~1,Wenhao Yan~1,Guojing Shen~1,Yongzhong Xing~(1*) 1 National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research,Huazhong Agricultural University,Wuhan 43.070,china 2 institute of subtropical {3.,chinese Academy of sciences,changsha 410125, china

FRIZZLE PANICLE(FZP ) is the major regulator to panicle *** elucidate its role in panicle branching and spikelet formation,the new fzp mutants (fzp-11) and FZP overexpression plants were used to analyze expression of the branching related genes and OsMADS box genes involved in the ABCDE model for floral *** fzp-11 had a single base mutation(A-T) in the AP2/ERF domain caused an amino acid *** the Mt panicle,the panicle branching

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Effects of increasing dietary rice straw level on chewing activity,ruminal fermentation,fibrolytic enzyme activity and blood biochemical profile in growing goats

Effects of increasing dietary rice straw level on chewing ac...

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4th International Symposium on Animal Nutrition,Health and Feed Additive

作者： M.Wang,X.G.Zhao~1,Z.L.Tan~*,S.X.Tang,Z.H.Sun,X.F.Han,C.S.Zhou Key Laboratory of Agro-ecological Processes of subtropical Region,Huanjiang Observation & Research Station of Karst Agro-ecosystem,institute of subtropical {3.,the chinese Academy of sciences,changsha P.O.Box 10,Hunan 410125,P.R.china

Effects of increasing dietary rice straw on the responses of goats were investigated by a 4×4 Latin Square *** goats were offered 1 of 4 diets with increasing proportion of rice straw（i.e.0.05,0.10,0.15 and 0.20 rice straw,respectively）.Dietary rice straw significantly increased（P<0.05） the time spent eating,ruminating and chewing *** pH value and acetate:propionate were significantly（P<0.05） increased in rumen, while the NH-H concentration was significantly（P<0.05） *** rice straw significantly affected（P<0.05） the some individual VFA concentration in rumen, such as increased molar proportion of acetate and isovalerate,and decreased molar proportion of *** enzymes activities in rumen were greatly influenced （P<0.05） by the increased dietary rice straw level,such as decreased CMCase,xylanase and cellobiase activities,and increased the avicelase *** blood biochemical parameters were hardly affected by the rice straw,except blood urea *** motilin firstly increased and then decreased with the highest value occurring at 8 h after feeding across four *** summary,increased rice straw has great impact on chewing activity and ruminal fermentation,and has little effect on energy metabolism in goats.

关键词： NDF ruminal fermentation motilin blood urea N fibrolytic enzyme Feeding behavior

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Protein synthesis and translation initiation activation in weaned piglets fed a low-protein diet supplemented with essential amino acids

Protein synthesis and translation initiation activation in w...

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2007年动物营养、保健与饲料添加剂国际学术研讨会

作者： D.Deng,W.Y.Chu,T.J.Li,R.L.Huang,K.Yao,Z.J.Liu,X.F.Kong, GY.Wu and Y.L.Yin~* a Laboratory of Animal Nutrition and Health and Key Laboratory of subtropical Agro-ecology,institute of subtropical {3.,The chinese Academy of sciences P.O.Box 10,changsha 410125

This study was conducted to determine the effects of a low-protein diet with essential amino acids（EAAs）(Lys,Met,Thr,Trp,Leu,Ile,or Val) on tissue protein fractional synthesis rate（FSR） and translation initiation factor activation in weaned *** three-line crossbred piglets（Duroc×Landrace×Yorkshine）,weaned at 21 days of age,were fed diets containing one of three crude protein（CP） levels（20.7,16.7,or 12.7%）.On d 10 of the trial all pigs were fitted with an indwelling jugular catheter for injecting L-Phe(1.5 mmol/kg body weight（BW） containing 0.6 mmol/kg BW L-[ring-H]Phe) and blood *** d 14 of the trial pigs were injected with with a flooding dose of L-Phe and tissue samples collected before all pigs were *** CP levels significantly affected（P3..08%（P3..97%and 3..88%)（P<0.01） respectively,than that in pigs fed the 20.7%CP diet..These results indicated that chronically feeding dietary protein to piglets lowers their known protein requirement and can reduce protein synthesis in tissues including the liver and skeletal muscle while changes in translation factor activation resulted decreased protein deposition.

关键词： Protein synthesis weaned piglets low-protein diet essential amino acids

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Serum Metabolome is Altered in Arginine-Supplemented Growing Pigs

Serum Metabolome is Altered in Arginine-Supplemented Growing...

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4th International Symposium on Animal Nutrition,Health and Feed Additive

作者： Q.H.He,X.F.Kong,Z.R.Tang,G.Y.Wu,H.R.Tang,P.P.Ren,F.H. Hao,R.L.Huang,T.J.Li,B.E.Tan,Y.N.Wu,and Y.L.Yin 3.Key Laboratory for Agro-ecological Processes in subtropical Region,institute of subtropical {3.,The chinese Academy of sciences,changsha,Hunan 410125,P.R.china 4 National institute for Nutrition and Food Safety,chinese CDC,Beijing 100050 china 5 The Graduate School of the chinese Academy of Science,Beijing 100049,china 6 Department of Animal Science,Texas A&M University,College Station,TX,77843.2471 7 State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,Wuhan Centre for Magnetic Resonance,Wuhan institute of Physics and Mathematics,the chinese Academy of sciences,Wuhan,Hubei 43.071,china

Arginine plays an important role regulating nutrient metabolism,but the underlying mechanisms are largely *** study was conducted to determine the effect of dietary arginine supplementation on the metabolome in serum of growing pigs using H nuclear magnetic resonance spectroscopy. Sixteen pigs（48±4 kg） were randomly assigned to one of 2 groups,representing supplementation with 0 or 1.0%of L-arginine-HCl to corn- and soybean meal-based *** was collected after 46 d *** arginine supplementation decreased fat deposition and increased protein accretion in the body. Principal component analysis showed that serum concentrations of low-density lipoprotein,very-low-density lipoprotein,and urea were lower,but concentrations of creatinine,tricarboxylic-acid-cycle metabolites,alanine,ornithine,lysine and tyrosine were greater in arginine-supplemented than in control pigs. Additionally,the arginine treatment affected serum concentrations of lipid signalling molecules（glycerophosphorylcholine and myo-inositol） and intestinal bacterial metabolites（formate,ethanol,methylamine, dimethylamine,acetate,and propionate）. These novel findings suggest that dietary arginine supplementation alters the catabolism of fat and amino acids in the whole body, enhances protein synthesis in skeletal muscle, and modulates intestinal microbial metabolism in growing pigs.

关键词： arginine metabolomics serum pigs nuclear magnetic resonance spectroscopy

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EFFECTS OF THE COVER AND INTERCROPPING ON FUNCTIONAL DIVERSITY OF SOIL MICROBIAL COMMUNITY OF TEA GARDEN

EFFECTS OF THE COVER AND INTERCROPPING ON FUNCTIONAL DIVERSI...

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第三世界生态高峰会

作者： Xu H.Q~(1,2) Xiao R.L~(**) Song T.Q~1 Zhou D.S~2 Luo W~2 Ren Q~2 Huang y~2 ~1Instinde of subtropical agriculture.chinese Academy of {3..Hunon:changsha,410125:~2Hunan Agricultural University,Hunan changsha,410128

Hilly red soil region along Yangtze River is a very important tea production place in china,However,Low organic matter content. nutrients shortage are the key problems for tea production in the area. Nowadays with the green barrier problem become more and more serious in tea marketplace,it is urgent to improve the tea garden environment by ecological *** to previous reports, straw mulching and intercrepping white clover could improve the ecological condition of soil water,fertility,aeration and thermal condition and so *** few reports concerned about the impact on soil microbial community of tea *** study tried to use BIOLOG bacterial identification system ECO plates to assess the *** experiment was constructed in the research field of the institute of subtropical {3.,chinese Academy of sciences, Hunan changsha,all the 6 treatments started from March,2004:1) QW(tillage without straw mulching or intercropping,chemical fertilized);2)QY(tillage without straw mulching or intercropping. with organic matter);3.DW(straw mulching and chemical fertilized);4)DY(straw mulching,with organic manure);5)SW(intereropping white clover and chemical fertilized);6)SY(intercropping white clover with organic manure).The soil samples were collected at the depth of 0-20em at 14,July,*** well color devilments (AWCD)data were calculated for each sample at each reading time,the result was:SY＞DW＞DY＞QY＞SW＞QW,this showed that organic manure can improve the abundance and diversity in different degrees. SY and DW were prior than others maybe because they can provide better C/N for soil microbial *** analysis on multiple indexes indicated that straw mulching and intercropping white clover have litter significant influence to the common Species of the soil microorganism,but the microbial community evenness *** the principal components analysis we found the differentiation to soil microbial community were brought mainly by the carb

关键词： BIOLOG Straw Mulching Intercropping White Clover Soil Microbial Community

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基于Penman-Monteith-Leuning遥感模型的西南喀斯特区域蒸散发估算

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应用生态学报 2018年第5期29卷 1617-1625页

作者：钟昊哲徐宪立张荣飞刘梅先中国科学院亚热带农业生态研究所长沙410125 中国科学院大学北京100049 中国科学院环江喀斯特生态系统观测研究站广西环江547100

西南喀斯特地区地形起伏大、土壤保水持水性差、生态环境脆弱.准确估算区域尺度蒸散发对研究喀斯特地区植被恢复、水资源管理等具有重要意义.本文以桂西北喀斯特植被恢复区为例,基于野外实测气象和蒸散发数据,采用最小二乘法对Penman-Mo... 详细信息

西南喀斯特地区地形起伏大、土壤保水持水性差、生态环境脆弱.准确估算区域尺度蒸散发对研究喀斯特地区植被恢复、水资源管理等具有重要意义.本文以桂西北喀斯特植被恢复区为例,基于野外实测气象和蒸散发数据,采用最小二乘法对Penman-Monteith-Leuning(PML)模型中气孔导度土壤湿度指数进行参数优化,并结合MOD15A2叶面积指数进行空间外推,实现了区域尺度上长时序蒸散发的估算.结果表明:研究区蒸散发模拟值与实测值有较好的一致性,模型确定系数、纳什系数和均方根误差分别为0.85、0.75和1.56 mm·d^(-1).蒸散发表现出季节变化特征,并与植被生长季物候特征一致,夏季蒸散量达到峰值.研究区年蒸散量在53.~103. mm,陆面蒸散发的空间分布呈现差异性,受降水的空间分布影响较大.年蒸散发可能不仅受到降水因素的影响,还与人类活动和土地利用类型有关.

关键词：蒸散发 Penman-Monteith公式叶面积指数尺度提升

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chinese Yam polysaccharide enhances growth performance and cellular immune response in weanling rats

Chinese Yam polysaccharide enhances growth performance and c...

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4th International Symposium on Animal Nutrition,Health and Feed Additive

作者： X.F.Kong~1,Y.Z.Zhang~(*1,4),Y.L.Yin~(**1),G.Y.Wu~(1,2),H.J.Zhou~3.Z.L.Tan~1,F. Yang~(1,4),M.J.Bo~(1,4),R.L.Huang~1,T.J.Li~(1,4),M.M Geng~1 1 Key Laboratory for Agro-ecological Processes in subtropical Region,institute of subtropical {3.,the chinese Academy of sciences,changsha,Hunan 410125,china 2 Department of Animal Science,Texas A&M University,College Station,TX 77843.USA 3.Department of Poultry Science,Texas A&M University,College Station,TX 77843.USA 4 Graduate School of the chinese Academy of sciences,Beijing 10003.,china

In order to develop a novel dietary additive for the potential application in domestic animals and human,one fraction of chinese Yam(CY) polysaccharide was *** study was conducted to determine the effects of dietary supplementing CY polysaccharide on the immune response and health growth in weaned *** supplementing CY polysaccharide increased the average daily feed intake and average daily gain,while decreased the feed-gain ratio overall after the supplementation compared with the non-additive *** polysaccharide also significantly increased the serum contents of IL-2 and TNF,as well as proliferation activity of peripheral lymphocytes,but decreased the serum contents of low density lipoprotein, total cholesterol and triglyceride compared with the non- supplemented *** increase in cellular immunity in the CY polysaccharide- supplemented weaned rats, conferring an important protective role in the non- specific defense against *** herbal polysaccharide,as a natural green dietary additive for promoting the immune response and healthy growth,may offer an effective alternative to antibiotics for weaned animals.

关键词： chinese Yam polysaccharide immune response performance

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拟南芥、荠菜DREB1A基因的克隆与植物表达载体的构建

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生物技术通报 2008年第4期24卷 99-103页

作者：杜洪伟陈芬肖国樱中国科学院亚热带农业生态研究所

用PCR方法从拟南芥和荠菜中分别克隆了DREB1A基因。序列分析发现从拟南芥中克隆的AtDREB1A基因与已发表的AtDREB1A基因序列(DQ3.253.)的同源性为99.69%,首次从荠菜中克隆的CbDREB1A基因序列(EF156749)与DQ3.253.的同源性为99.54%。利用... 详细信息

用PCR方法从拟南芥和荠菜中分别克隆了DREB1A基因。序列分析发现从拟南芥中克隆的AtDREB1A基因与已发表的AtDREB1A基因序列(DQ3.253.)的同源性为99.69%,首次从荠菜中克隆的CbDREB1A基因序列(EF156749)与DQ3.253.的同源性为99.54%。利用这两个基因分别构建了两个诱导表达载体(prd29A/AtDREB1A,prd29A/CbDREB1A)和两个组成型表达载体(pCaMV3.S/AtDREB1A,pCaMV3.S/CbDREB1A),以便进一步开展植物抗旱基因工程研究。

关键词： DREB1A基因拟南芥荠菜基因克隆表达载体构建

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