检索结果-南通市图书馆

Highly Sensitive Fluorescent-labeled Probes and Glass Slide Hybridization for the Detection of Plant RNA Viruses and a Viroid

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta biochimica et biophysica Sinica 2007年第5期39卷 326-334页

作者： Zhiyou DU~(1,2., Bo JIN~3, Wenhong LIU~4, Liang CHEN~1, and Jishuang CHEN~(2.1*) 1 College of Life Sciences, zhejiang university, hangzhou 3.002., {2. 2.institute of bioengineering, zhejiang Sci-Tech university, hangzhou 310018, {2. 3 College of Life Sciences, zhejiang Chinese Medical university, hangzhou 310053, {2. 4 College of bioengineering, zhejiang Chinese Medical university, hangzhou 310053, {2. College of Life Sciences Zhejiang UniversityHangzhou 310029 China institute of bioengineering Zhejiang Sci-Tech UniversityHangzhou 310018 China College of Life Sciences Zhejiang Chinese Medical UniversityHangzhou 310053 China College of bioengineering Zhejiang Chinese Medical UniversityHangzhou 310053 China

In this study, a modified method of the conventional RNA dot-blot hybridization was established, by replacing32. labels with CY5 labels and replacing nylon membranes with positive-charged glass slides, for detecting plant RNA viruses and a viroid. The modified RNA dot-blot hybridization method was named glass slide hybridization. The optimum efficiency of RNA binding onto the surfaces of activated glass slide was achieved using aminosilane-coated glass slide as a solid matrix and 5×saline sodium citrate （SSC） as a spotting solution. Using a CY5-labeled DNA probe prepared through PCR amplification, the optimized glass slide hybridization could detect as little as 1.71 pg of tobacco mosaic virus （TMV） RNA. The sensitivity of the modified method was four times that of dot-blot hybridization on nylon membrane with a32.-labeled probe. The absence of false positive within the genus Potyvirus [potato virus A, potato virus Y （PVY） and zucchini yellow mosaic virus] showed that this method was highly specific. Furthermore, potato spindle tuber viroid （PSTVd） was also detected specifically. A test of 40 field potato samples showed that this method was equivalent to the conventional dot-blot hybridization for detecting PVY and PSTVd. To our knowledge, this is the first report of using dot-blot hybridization on glass slides with fluorescent-labeled probes for detecting plant RNA viruses and a viroid.

关键词： glass slide hybridization fluorescent labeling dot-blot hybridization virus viroid

Recombinant Functional Human Lactoferrin Expressed in Baculovirus System

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta biochimica et biophysica Sinica 2006年第3期38卷 201-206页

作者： Tao LIU~(1,2. Yao-Zhou ZHANG~2. Xiang-Fu WU~31 institute of biochemistry,College of Life Sciences,{2.,hangzhou 3.002.,china 2.institute of biochemistry,zhejiang Sci-Tec university,hangzhou 310018,china 3.institute of biochemistry and Cell biology,Chinese Academy of Sciences Shanghai 2.0031,china institute of biochemistry College of Life Sciences Zhejiang UniversityHangzhou 310029 China institute of biochemistry Zhejiang Sci-Tec UniversityHangzhou 310018 China institute of biochemistry and Cell biology Chinese Academy of SciencesShanghai 200031 China

Human lactoferrin (hLf) is a multifunctional iron-binding *** this study,weamplified hLfcDNA by reverse transcription-polymerase chain reaction from normal human mammary *** nucleotide sequence of the hLf was identical to the known *** constructed a recombinant virus,vBm-hLf,harboring the hLf gene and exploited the BmN cells as host to produce recombinant human lactoferrin(rhLf).It was found that a recombinant protein with a molecular mass of approximately 78 kDa was *** 13.5μg rhLf was purified from 1-2.10～5 BmN cells infected by vBm-hLf and the rhLfproved to be biologically *** method established in our study will pave the way for efficient productionof rhLf for further application of this protein in the future.

关键词： bacteriostatic activity baculovirus Bombyx mori human lactoferrin reverse transcriptionpolymerase chain reaction

Effect of background electrolytes on the adsorption of nitroaromatic compounds onto bentonite

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of environmental Sciences 2009年第8期21卷 1044-1052页

作者： CHEN Baoliang, HUANG Wenhai Department of environmental Science, zhejiang university, {2., zhejiang, china. Department of environmental Science Zhejiang University Hangzhou 310028 Zhejiang China

To further elucidate interaction of nitroaromatic compounds with mineral surface, the sorption of m-dinitrobenzene （m-DNB） and nitrobenzene to original bentonite in aqueous solution containing different electrolytes （i.e., KCl, NH4Cl, CaCl2.and Tetramethylammonium bromide （TMAB）） was studied. The sorption of m-DNB was greatly enhanced with the presence of KCl and NH4Cl, while little influence was observed with CaCl2.and TMAB, following the order of KCl 〉 NH4Cl 〉〉 TMAB, CaCl2. or DI water. For nitrobenzene, sorption enhancement only occurred at high nitrobenzene concentrations in the presence of KCl, and the solute equilibrium concentration at inflexion point was lowered with increasing KCl concentration. These sorption enhancements were significantly promoted with the increase of electrolyte concentration. The salting-out effect is insufficient to account for the sorption enhancement by original bentonite with increasing KCI or NH4Cl concentration. X-ray diffraction patterns of bentonite suspensions indicated that the sorption enhancement of m-DNB was attributed to the intercalation of K^＋ or NH4^＋ into bentonite interlayer and then dehydration with m-DNB to form inner-sphere complexes, which caused previously expanded bentonite interlayers to collapse in aqueous suspension, thus further enhanced the interaction of phenyl with siloxane surface. In comparison, the sorption enhancement of NB is attributed to the formation of outer-sphere complexes with K^＋ at high solute-loadings （〉 2.（0-400 mg/kg）. The sorption of m-DNB to initially modified TMA^＋-bentonite and K^＋-bentonite was almost the same as respective sorption to original bentonite in solution containing TMA^＋ and K^＋.

关键词： sorption nitroaromatic compound bentonite cation type wastewater treatment

Interaction mechanisms of organic contaminants with burned straw ash charcoal

在线全文

学校读者我要写书评

暂无评论

Journal of environmental Sciences 2010年第10期22卷 1586-1594页

作者： Wenhai Huang,Baoliang Chen Department of environmental Science,zhejiang university,{2.,china Department of environmental Science Zhejiang University Hangzhou 310028 China

Black carbons （e.g., charcoal） have a great impact on the transport of organic contaminants in soil and water because of its strong affinity and ubiquity in the environment. To further elucidate their interaction mechanism, sorption of polar （p-nitrotoluene, m-dinitrobenzene and nitrobenzene） and nonpolar （naphthalene） aromatic contaminants to burned straw ash charcoal under different de-ashed treatments were investigated. The sorption isotherms fitted well with Freundlich equation, and the Freundlich N values were all around 0.31-0.38, being independent of the sorbate properties and sorbent types. After sequential removal of ashes by acid treatments （HCl and HCl-HF）, both adsorption and partition were enhanced due to the enrichment of charcoal component. The separated contribution of adsorption and partition to total sorption were quantified. The effective carbon content in ash charcoal functioned as adsorption sites, partition phases, and hybrid regions with adsorption and partition were conceptualized and calculated. The hybrid regions increased obviously after de-ashed treatment. The linear relationships of Freundlich N values with the charring-temperature of charcoal or biochar （the charred byproduct in biomass pyrolysis） were observed based on the current study and the cited publications which included 15 different temperatures （100-850℃）, 10 kinds of precursors of charcoal/biochar, and 10 organic sorbates.

关键词： ash charcoal organic pollutant adsorption partition effective carbon

Expression,Characterization and Immunogenicity of a Major Outer Membrane Protein from Vibrio alginolyticus

在线全文

学校读者我要写书评

暂无评论

Acta biochimica et biophysica Sinica 2007年第3期39卷 194-200页

作者： Ronghua QIAN~(1,2. Wuying CHU~3 Zhijuan MAO~(1,4) Chongwen ZHANG~(1,2. Yongwei WEI~2.Lian YU~2. 1 Department of biomedical Engineering,zhejiang university,{2.,china 2.institute of Preventive Veterinary Medicine,zhejiang university,{2.,china 3.institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 41012.,china 4 Ningbo Oceanography and Fishery institute,Ningbo 315012.china Department of biomedical Engineering Zhejiang UniversityHangzhou 310027 China institute of Preventive Veterinary Medicine Zhejiang UniversityHangzhou 310029 China institute of Subtropical Agriculture Chinese Academy of SciencesChangsha 410125 China Ningbo Oceanography and Fishery instituteNingbo 315012 China

Vibrio alginolyticus is one of the Vibrio pathogens common to humans and marine *** infection and induction of the host immune response,outer membrane proteins of bacteria play animportant *** this study,an outer membrane protein gene(ompW)was cloned from *** andexpressed in Escherichia *** 645 bp open reading frame(ORF)encodes a protein of 2.4 amino acidresidues with a predicted molecular weight of 2..3 *** amino acid sequence showed a high identitywith that of Photobacterium damselae(96.2.)and Vibrio parahaemolyticus(94.4%).The alignment analy-sis indicated that OmpW was highly *** dodecyl sulfate-polyacrylamide gel electrophoresisshowed that the gene was over-expressed in *** BL2.(DE3).Western blot analysis revealed that theexpressed protein had *** recombinant protein was purified by affinity chromatographyon Ni-NTA Superflow *** yellow croaker vaccinated with the purified OmpW showed significantlyincreased antibody to OmpW,which could resist the infection by ***.A specific antibody wasdetected by enzyme-linked immunosorbent *** study suggested that the conserved OmpW could bean effective vaccine candidate against infection by ***.

关键词： Vibrio alginolyticus ompW expression characterization immunogenicity vaccine candidate

Treatment of turtle-breeding wastewater and domestic fecal sewage with soil cultivating system

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of environmental Sciences 2001年第3期13卷 342-345页

作者： LAO Shan\|gen(Department of environmental Engineering, zhejiang university,{2., china) 浙江大学浙江杭州 310029

Turtle-breeding wastewater and domestic fecal sewage were treated by means of soil cultivating system. Results indicated that more than 50% CODCr and BOD5, of wastewaters were removed, removal rates of NH4+ -N could reach about 70%-80%, but PO43- could not be removed. The thesis analyzed functional mechanisms for pollutants and put forward main elements affecting treatment efficiencies, thus provided conditions for further research.

关键词： turtle-breeding wastewater fecal sewage soil CODCr BOD5 N P

Mechanisms of granular activated carbon anaerobic fluidized-bed process for treating phenols wastewater

在线全文

学校读者我要写书评

暂无评论

Journal of environmental Sciences 2002年第1期14卷 132-135页

作者： LAO Shan gen (Department of environmental Engineering, zhejiang university, {2., china) Department of environmental Engineering Zhejiang University Hangzhou 310029

Granular activated carbon (GAC) anaerobic fluidized bed reactor was applied to treating phenols wastewater. When influent phenol concentration was 1000 mg/L, volume loadings of phenol and COD Cr were 0 39 kg/(m 3·d) and 0 98 kg/(m 3·d), their removal rates were 99 9% and 96 4% respectively. From analyzing above results, the main mechanisms of the process are that through fluidizing GAC, its adsorption is combined with biodegradation, both activities are brought into full play, and phenol in wastewater is effectively decomposed. Meanwhile problems concerning gas liquid separation and medium plugging are well solved.

关键词： granular activated carbon (GAC) anaerobic fluidized bed phenol removal rate

Quasi-static Analysis of Thrust-loaded Angular Contact Ball Bearings Part Ⅱ:Results and Discussion

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Mechanical Engineering 2012年第1期25卷 81-87页

作者： CHEN Wenhua1,2.MA Zikui1,GAO Liang1,LI Xinglin3,and PAN Jun2.1 institute of Mechanical Design,zhejiang university,{2.,china 2.institute of Mechanical Design and Manufacturing,zhejiang Sci-Tech university,hangzhou 310018,china 3 State Testing Laboratory of hangzhou Bearing Test & Research Center,{2.,china institute of Mechanical Design Zhejiang University Hangzhou China institute of Mechanical Design and Manufacturing Zhejiang Sci-Tech University Hangzhou China State Testing Laboratory of hangzhou Bearing Test & Research Center Hangzhou China

Ball bearings are widely employed mechanical components characterized by high precision and quality,and usually play important roles in various rotary machines and *** advanced applications require a deep understanding of their various kinematic and tribological characteristics that are essential to predict the fatigue endurance,relieve the vibration and minimize the power dissipation of ball bearings in particular *** angular contact ball bearing under a specified operating condition is simulated with the quasi-static/creepage analytical model proposed in the preceding *** results demonstrate that the ball bearing is a statically determinate *** the balls spin on both inner and outer races means the ball is controlled by neither the inner nor the outer *** friction between the ball and raceway renders the inner and outer contact angles *** larger the coefficient of friction is,the larger the angle *** tangential traction perpendicular to the rolling direction due to the spin induces a gyro-like rotation of the ball with respect to the raceway even if no inertial effects are *** tangential elastic compliance of contacting surfaces gives rise to locked areas within the contact patch and transforms the sliding lines from circles into *** differential slip due to the close conformity of the ball and raceway makes the sliding and traction distributions asymmetric,which will influence the location of the spinning center of the ball with respect to the *** quasi-static/creepage model can be used to reveal the operating behaviors of ball bearings running under steady conditions and to optimize the design of ball bearings for specific applications.

关键词： ball bearing quasi-static creepage differential slip tangential elastic compliance

Synthesis and crystal structure of complex of Ce(NO_3)_3 with N-(3-methyl-pyridin-2.yl)-formimidoyl-(3-methyl-pyridin-2.yl) hydrazone

在线全文

学校读者我要写书评

暂无评论

Science china Chemistry 2004年第3期47卷 251-256页

作者： JIANG Yinzhi1,2.& HU Weixiao1 1. Department of Pharmaceutical Science, zhejiang university of Technology, hangzhou 310032. {2. 2. Department of Applied Chemistry, zhejiang institute of Science and Technology, hangzhou 310033, {2. Department of Pharmaceutical Science Zhejiang University of Technology Hangzhou China Department of Applied Chemistry Zhejiang Institute of Science and Technology Hangzhou China

The reaction of 3,6-di-(3-methyl-pyridin-2.yI)-s-tetrazine (DMPTZ, II) with CeIII salt [Ce(NO3)3 · 6H2.] generates a new ligand, N-(3-methyl-pyridin-2.yl)-formimidoyl-(3-methyl-pyridin-2.yl) hydrazone (L), and forms a new complex: a mononuclear complex [Ce(L)(NO3)2.(H2.)3] · NO3 (III). Crystal data for III: space group P-1, with a = 0.7133(4) nm, b = 1.1139(2. nm, c = 1.4572.3) nm, α= 102.13(2.°, β= 99.81(3)°, γ= 91.10(3)°, Z = 2. V = 1113.6(7) nm3, μ = 2.12. mm?1 and F(000) = 630. L acts as a tri-dentate chelating ligand in III. There are 10 coordination sites around Ce3+ of III, which are respectively occupied by seven oxygen atoms (four from two nitrate anions and three from three H2. molecules) and three nitrogen atoms (all from L). The cerium atom and three chelating nitrogen atoms are coplanar. The mechanism of the metal assisted decomposition is discussed briefly.

关键词： rare earth, complex, crystal structure, synthesize, 3,6-bis (3-methyl-pyridin-2.yl)-s-tetrazine, N-(3-methyl-pyridin- 2.yl)-formimidoyl-(3-methyl-pyridin-2.yl) hydrazone.