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Antioxidant effects of green tea polyphenols

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Chinese science Bulletin 2003年第4期48卷 315-319页

作者： ZHAO Baolu Laboratory of Visual Information Processing, research Centers of Brain and Cognitive science, institute of Biophysics, Chinese academy of sciences, Beijing 100101, china Laboratory of Visual Information Processing Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China

The unprecedented interest in the antioxidant activity of green tea polyphenols (GTP) is due to the appar-ent health benefits of tea drinking and the experimental re-sults with the polyphenols. The results suggest that the gal-loylated catechins show stronger antioxidant effect than that of nongalloylated catechins and the double bond in C ring also plays an important role in this effect. There are synergic effects between different catechins and the antioxidant effect of (+)-epimers is stronger than that of (-)-epimers. The active site to scavenge free radicals mainly locates in p-hydroxyl of phenol in gallic acid and hydroxyl of phenol in the pyrane also shows activity. The large p bond in chromane and benzene ring of catechin is the structure base for their anti-oxidant effects. The prevent effect of GTP against diseases and their redox regulation in cell signal pathway are very important to be studied in the future.

关键词：绿茶茶多酚天然抗氧化剂抗氧化作用生物类黄酮自由基抗衰老

Effect of Waterlogged and Aerobic Incubation on Enzyme Activities in Paddy Soil

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Pedosphere 2006年第4期16卷 532-539页

作者： WANG Xiao-Chang LU Qin Department of tea science College of Agriculture and Biotechnology Zhejiang University Hangzhou 31002g （China） institute of Soil science Chinese Academy of Sciences Nanjing 210008 （China）

An incubation experiment with soil water content treatments of 0.15 （W1）, 0.20 （W2）, and 0.40 （W3） g g^-1 soil was carried out for two months to investigate the activities of important enzymes involved in C, N, P, and S cycling in a paddy soil from the Taihu Lake region, china, under waterlogged and aerobic conditions. Compared with air-dried soil, waterlogging resulted in a significant decrease （P ≤ 0.05） of fluorescein diacetate （FDA） and /3-D-glucosidase activities, and this effect was enhanced with increasing waterlogging time. Waterlogging also significantly inhibited （P ≤ 0.05） arylsulfatase as well as alkaline and acid phosphatase activities, but did not decrease the activities with the increase in waterlogging time. Short-term waterlogging did not affect urease activity, but prolonged waterlogging decreased it markedly. In contrast, the aerobic incubation （W1 and W2 treatments） significantly increased （P ≤ 0.05） FDA, alkaline phosphatase, and /3-D-glucosidase activities. With aerobic treatments the activities of FDA and alkaline phosphatase increased with incubation time, whereas /3-D-glucosidase activity decreased. A significant difference （P ≤ 0.05） was usually observed between the W1 and W2 treatments for the activities of FDA as well as alkaline and acid phosphatase; however,/3-D-glucosidase and urease were usually not significant （P ≤ 0.05）. No activity differences were observed between waterlogging and aerobic incubation for arylsulfatase and urease.

关键词： aerobic incubation enzyme activity paddy soil waterlogging

Beta-Glucosidase Activity in Paddy Soils of the Taihu Lake Region, china

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Pedosphere 2006年第1期16卷 118-124页

作者： WANG Xiao-Chang LU Qin Department of tea science Zhejiang University Hangzhou 310029 （China）. institute of Soil science Chinese Academy of Sciences Nanjing 210008 （China）

The profile distribution of β-gulcosidase activity in twelve typical paddy soil profiles with high productivity in the Taihu Lake region of china were investigated. Activities of β-gulcosidase in the plow layers were in the range of 52.68- 137.02μg PNP g^-1 soil h^-1 with a mean of 89.22μg PNP g^-1 soil h^-1. However, most plow layers ranged from 70 to 110 μg PNP g^-1 soil h^-1. The profile distribution of β-gulcosidase activity in the 12 soil profiles decreased rapidly with soil depth, with activity at the 60 cm depth only about 10% of that in the surface layers （0-15 cm or 0-20 cm）. In these soil profiles, β-gulcosidase activity was significantly positively correlated with soll organic carbon and arylsulphatase activity. Meanwhile, a significantly negative correlation was shown between β-gulcosidase activity and soil pH.

关键词： beta-gulcosidase activity profile distribution paddy soil Taihu Lake region

VARIETAL CHARACTERS AND GENETIC VARIATIONS OF OOLONG tea GERMPLASMS

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VARIETAL CHARACTERS AND GENETIC VARIATIONS OF OOLONG TEA GER...

2005 International Symposium on Innovation in tea science and Sustainable Development in tea Industry

作者： GUO Jichun tea research institute, Fujian academy of Agricultural science, Fu’an 355000, china

＜正＞Oolong tea and its germplasms are only owned in china, which distribute in Fujian, Guangdong and Taiwan. Fujian is the original place and main production area. Oolong tea cultivars are the pioneer of individual selection and asexual reproduction, whose aroma and some other quality traits are distinctness and stability. Studying on oolong tea varietal character and genetic variation is very significative for enriching tea plant breeding theory and guiding tea plant breeding practice. Distribution and type of oolong tea germplasms and basic characters of agronomical traits, processing quality traits, anatomical structure of leaf, isoenzymes of esterase （EST）, biochemical components and aroma components, variation of intraspecific cross compatibility, genetic variations of seed traits, seedling traits, leaf-expanding period, young shoot color, processing quality and aroma components of oolong tea germplasms were reviewed in this paper. Synchronously, some selecting indices of young shoot color, percentage of high quality tea, anatomical structure of leaf, biochemical components and aroma components traits were put forward. Moreover, a viewpoint that the strip area along 25°N, from east to west in china contains some germplasms suitable for oolong tea was put forward.

关键词： Oolong tea germplasms varietal character genetic variation

Difference in Selenium Accumulation in Shoots of Two Rice Cultivars

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Pedosphere 2006年第5期16卷 646-653页

作者： ZHANG Lian-He SHI Wei-Ming WANG Xiao-Chang State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of SciencesNanjing 210008 （China） Agricultural College Henan University of Science and Technology Luoyang 471003 （China） Department of tea science Zhejiang University Hangzhou 310029 （China）

Two japonica rice （Oryza sativa L.） cultivars, Xiushui 48 and S. Andrea, differing in their ability to accumulate Se in the grain （as high as a three-fold difference）, were compared for selenium （Se） accumulation in their shoots when their growth media was supplied with different forms of Se. Results indicated that when treated with 0.25μmol L^-1 Na2SeO3, Xiushui 48 accumulation of Se in the shoots was significantly more rapid （P〈0.05） than S. Andrea, probably because of greater Se uptake and transport in Xiushui 48. Xiushui 48 rice seedlings had a higher shoot-Se accumulation rate and absorbed selenocysteine （Se-Cys） more rapidly than S. Andrea seedlings. However, when treated with Se as 0.25μmol L^-1 selenomethionine （Se-Met）, the S. Andrea seedlings＇ accumulation rate was significantly greater （P〈0.05） than that of Xiushui 48. Possibly, the high Se accumulation rate of Xiushui 48 seedling shoots compared to S. Andrea shoots was the result of a higher capacity of Xiushui 48 to transform selenite to organic Se compounds and a higher selenite uptake rate.

关键词： rice cultivars Se accumulation selenite transport uptake rate

The cessation and detoxification effect of tea filters on cigarette smoke

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science china(Life sciences) 2010年第5期53卷 533-541页

作者： YAN JingQi1,2,DI XiaoJing1,4,LIU CaiYi3,ZHANG HuiMin3,HUANG XiouQin3,ZHANG JunJing1,2,ZHAO Yan5,ZHANG LongZe1,CHANG YanZhong4,LIANG YongLin2$,TAO Ran3 & ZHAO BaoLu1,21 State Key Laboratory of Brain and Cognitive science,institute of Biophysics,Chinese academy of sciences,Beijing 100101,china 2 Key Laboratory of Mental Health,institute of Psychology,Chinese academy of sciences,Beijing 100101,china 3 Addiction Branch,Beijing Military Region General Hospital,Beijing 100700,china 4 institute of Molecular Neurobiology,Hebei Normal University,Shijiazhuang 050016,china 5 Department of Food sciences and Technology,Harbin institute of Technology at Weihai,Weihai 264209,china State Key Laboratory of Brain and Cognitive science Institute of Biophysics Chinese Academy of Sciences Beijing China Key Laboratory of Mental Health Institute of Psychology Chinese Academy of Sciences Beijing China institute of Molecular Neurobiology Hebei Normal University Shijiazhuang China Addiction Branch Beijing Military Region General Hospital Beijing China Department of Food sciences and Technology Harbin Institute of Technology at Weihai Weihai China

To treat tobacco addiction,a tea filter was developed and studied for smoking *** work reports the smoking cessation effect of tea when it was used as a component of cigarette *** one trial it was found that after using the tea filters for 2 months,the volunteer smokers decreased their cigarette consumption by 56.5%,and 31.7% of them stopped *** work identified a new method and material,tea filter and theanine,which inhibit tobacco and nicotine addiction and provide an effective strategy for treating tobacco addiction.

关键词： cigarette smoking addiction cigarette cessation tea filter theanine nicotine dependence nicotine acetylcholine receptor(nAChR) dopamine public health free radical

ANTIOXIDANT PHENOLIC COMPOUNDS IN PU-ER tea

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ANTIOXIDANT PHENOLIC COMPOUNDS IN PU-ER TEA

2005 International Symposium on Innovation in tea science and Sustainable Development in tea Industry

作者： LIN Zhi, LV Haipeng, CUI Wenrui, SHE Gaimei, ZHANG Yingjun and YANG Chongren State Key Laboratory of Phytochemistry and Plant Resources in West china, Kunming institute of Botany, Chinese academy of sciences, Kunming, 650204, china Key Laboratory of tea Chemical Engineering, Ministry of Agriculture, tea research institute, Chinese academy of Agricultural science, Hangzhou, 310008, china

＜正＞Eleven phenolic compounds were isolated from the EtOAc extract of Pu-Er tea, which was produced by a post fermented process using the crude green tea prepared from the leaves of Camellia sinensis var. assamica in Mengku, Shuangjiang county, Yunnan Province. Their structures were identified as gallocatechin （1）, （-）-epicatechin （2）, （+）- catechin （3）, myricetin （4）, gallic acid （5）, kaempferol （6）, kaempferol-3-O- -D-glucopyranoside （7）, kaempferol-3-O-rutinoside （8）, quercetin （9）, quercetin-3- -D-glucopyranoside （10）, 2,5-dihydroxy- benzoic acid （11）. Compounds 6, 7, 8, 9, 10, 11 were firstly isolated from Pu-Er tea. All of isolated compounds were tested for their antioxidant activities by DPPH radical scavenging assay. It was found that compounds 2, 3 and 5 showed antioxidant activity closed with that of positive control, L-ascorbic acid, and the flavonoids （5, 6 and 9） are more active than the flavonoid glycosides （7, 8 and 10） and compound 11, indicating that gallic acid and simple catechins were main antioxidant phenolic compounds in Pu-er tea.

关键词： Camellia sinensis var. assamica Pu-Er tea phenolic compounds antioxidant

THE INTERACTION BETWEEN tea CULTURE AND tea ECONOMY

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THE INTERACTION BETWEEN TEA CULTURE AND TEA ECONOMY

2005 International Symposium on Innovation in tea science and Sustainable Development in tea Industry

作者： ZHENG Naihui, YANG Jiangfan, YE Naixing institute of tea, Fujian academy of Agricultural sciences, Fu’an, Fujian 355015, china institute of science, Technology and Economy of tea Industry, FAFU, Fuzhou, Fujian 350002, china

＜正＞The relationship between culture and economy was discussed in the paper. It can be described as that culture and economy are supplementary to each other, restricted by each other, and interacted each other as well. The development of tea culture is helpful to promote the development of tea economy, which will further enhance tea production.

关键词： tea culture economy

Potential of Industrial Byproducts in Ameliorating Acidity and Aluminum Toxicity of Soils Under tea Plantation

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Pedosphere 2010年第5期20卷 645-654页

作者： LI Jiu-Yu WANG Ning XU Ren-Kou D. TIWARI State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 (China) Department of Chemistry Mizoram University Tanhril Campus Aizawl 796009 (India) Graduate University of the Chinese academy of sciences Beijing 100049 (China)

It is imperative to choose some low cost, available and effective ameliorants to correct soil acidity in southern china for sustainable agriculture. The present investigation dealt with the possible role of industrial byproducts, i.e., coal fly ash （CFA）, alkaline slag （AS）, red mud （RM） and phosphogypsum （PG） in correcting acidity and aluminum （Al） toxicity of soils under tea plantation using an indoor incubation experiment. Results indicated that CFA, AS and RM increased soil pH, while PG decreased the pHs of an Ultisol and an Alfisol. The increment of soil pH followed the order of RM 〉 AS 〉 CFA. All the industrial byproducts invariably decreased exchangeable Al and hence increased exchangeable Ca, Mg, K and Na and effective cation exchange capacity. RM, AS and lime decreased total soluble Al, exchangeable Al and organically bound Al. Formation and retention of hydroxyl-Al polymers were the principal mechanism through which Al phytotoxicity was alleviated by application of these amendments. In addition, the heavy metal contents in the four industrial byproducts constituted a limited environmental hazard in a short time at the rates normally used in agriculture. Therefore, the short-term use of the byproducts, especially AS and RM, as amendments for soil acidity and AI toxicity in acid soils may be a potential alternative to the traditional use of mined gypsum and lime.

关键词： acid soil alkaline slag amelioration coal fly ash red mud