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Polymer-assisted nanoparticulate contrast-enhancing materials

Science china chemistry 2010年第3期53卷 479-486页

作者： DING Yin1,3,HU Yong2.WU Wei1 & JIANG XiQun1 1laboratory of Mesoscopic chemistry and Department of Polymer Science and Engineering,College of chemistry & chemical Engineering,nanjing university,nanjing 210093,china 2.ational laboratory of Solid State Microstructure,Department of Material Science and Engineering,nanjing university,nanjing 210093,china 3.ey laboratory of analytical chemistry for life Science,ministry of education,school of chemistry and chemical Engineering,nanjing university,nanjing 210093,china laboratory of Mesoscopic chemistry and Department of Polymer Science and Engineering College of Chemistry & Chemical Engineering Nanjing University Nanjing China Key laboratory of {2. chemistry for life Science Ministry of Education School of Chemistry and Chemical Engineering Nanjing University Nanjing China National laboratory of Solid State Microstructure Department of Material Science and Engineering Nanjing University Nanjing China

The application of nanotechnology in medicine research has significant potential in modern biomedical research,disease diagnosis and *** fluorophore-based detection techniques have been widely used as imaging and signal transduction tools for the detection of trace levels of *** photosensitivity of the fluorophores,however,limits their application in such complex environments as living bio-systems where degradation or photobleaching *** nanoparticles have unique and stable optical,electrical,magnetic and catalytic properties because of their various shapes,sizes and *** potential of the nanoparticles as novel imaging agents has been studied as a possible solution to this *** modification of inorganic nanoparticles could enhance their biostability in physiological conditions and ability of targeting bioactive molecules.

关键词： contrast agent cell image gold nanoparticles iron oxide nanoparticles carbon nanotubes polymeric-inorganic hybrid nanoparticles

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Optical applications of quantum dots in biological system

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Science china chemistry 2011年第8期54卷 1177-1184页

作者： WANG Wei & ZHU Jun-Jie State Key laboratory of {2. chemistry for life Science school of chemistry and chemical Engineering, nanjing university, nanjing 210093, china State Key laboratory of {2. chemistry for life Science school of chemistry and {2. Engineering Nanjing University Nanjing China

This review presents a simple introduction on the unique properties and general synthesis of quantum dots (QDs) in which we lay emphasis on the optical applications in the biological system. The detection of biological molecules such as DNA, protein and enzyme, the cell-based analysis and in vivo animal imaging are mainly discussed.

关键词： quantum dots optical application biological system

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Rhodamine B doped silica nanoparticle labels for protein microarray detection

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Science china chemistry 2010年第4期53卷 747-751页

作者： WANG Ying1, LI ZhongHui1, ZHONG WenYing2. LI Hui2. XU DanKe1 & CHEN HongYuan1 1Key laboratory of {2. chemistry for life Science, ministry of education school of chemistry and chemical Engineering, nanjing university, nanjing 210093, china 2.epartment of {2. chemistry, china Pharmaceutical university, nanjing 2.0009, china Key laboratory of {2. chemistry for life Science Ministry of Education school of chemistry and {2. Engineering Nanjing University Nanjing China Department of analytical chemistry China Pharmaceutical University Nanjing China

A core-shell Rhodamine B-doped SiO2.nanoparticle was synthesized and its fluorescent intensity was found to be 1000 times higher than that of individual Rhodamine B molecule. The doped nanoparticles were further conjugated with streptavidin and the resulting nanoparticles were used in the detection of reverse-phase protein microarrays, in which human IgG of various concentrations was first immobilized on aldehyde-modified glass slides and then biotinlyated goat anti human IgG as well as the labeled nanoparticles were sequentially conjugated. The calibration curve is linear over the range from 800 fg to 500 pg and the limit of detection is 100 fg, which is 8 times lower than that of streptavidin-labeled Cy3 fluorescent dyes. The dyedoped SiO2.nanoparticles show potentials for the protein array detection.

关键词： Rhodamine B SiO2 nanoparticles protein detection protein microarrays

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Magnetic particles and cadmium sulfide nanoparticles tagging for signal-amplifying detection of nucleic acids

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Science china chemistry 2011年第8期54卷 1304-1310页

作者： DONG XiaoYa, ZHAO WeiWei, XU JingJuan & CHEN HongYuan State Key laboratory of {2. chemistry for life Science school of chemistry and chemical Engineering, nanjing university, nanjing 210093, china State Key laboratory of {2. chemistry for life Science school of chemistry and {2. Engineering Nanjing University Nanjing China

A versatile DNA recognition system using cadmium sulfide nanoparticles (CdS NPs) as labels was proposed for ultrasensitive detection of specific sequence DNA based on target recycling. This strategy utilized the magnetic particles (MNPs) for the immobilization of linker DNA and CdS NPs and the subsequent target DNA hybridization. Using the unique characteristic of nicking endonuclease for cutting one specific strand of double strand DNA (ds DNA), the linker DNA could be transected and the target DNA could be liberated for re-hybridization and hence the amount of released CdS NPs was enhanced. Due to the advantage of the MNPs and signal amplification from the target recycling, the analyte DNA could be detected by the square-wave stripping voltammetry (SWV) in a wide linear range from 0.4 fM to 100 fM with the detection limit down to 0.08 fM. The proposed DNA detection strategy possesses high sensitivity, satisfactory reproducibility and excellent stability, which might have potential in other DNA biological assays.

关键词： CdS nanoparticles square wave stripping voltammetry nicking endonuclease electrochemical DNA assay

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Study on specific interaction of new ferrocene-substituted carborane conjugates with hemoglobin protein

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Science china chemistry 2012年第4期55卷 594-603页

作者： WU ChunHui1,3, YE HongDe2. JIANG Hui1, WANG XueMei1 & YAN Hong2.1State Key Lab of Bioelectronics (Chien-Shiung Wu Lab), Southeast university, {2. 2.0096, china 2.tate Key laboratory of Coordination chemistry school of chemistry and chemical Engineering, nanjing university, nanjing 210093, china 3school of life Science and Technology, university of Electronic Science and Technology of china, Chengdu 610054, china State Key Lab of Bioelectronics (Chien-Shiung Wu Lab) Southeast University Nanjing China school of life Science and Technology University of Electronic Science and Technology of China Chengdu China State Key laboratory of Coordination {2. school of chemistry and {2. Engineering Nanjing University Nanjing China

The interactions between the new organometallic complexes, ferrocenesubstituted dithioocarborane conjugates （denoted as FcSB1, FcSB2.and FcSBCO） and hemoglobin （Hb） are investigated by electrochemistry, fluorescence and UVvis absorption spectroscopy. The results demonstrate that FcSB1, FcSB2.and FcSBCO can bind to the heme iron center through the replacement of the weakly bound H2./02.in the distal heme pocket of Hb by their sulfur donor atoms, inducing the allosteric change from the R state （oxygenated conformation, relax） to T state （deoxygenated conformation, tense）. The binding affinity is in the order of FcSBCO〉FeSB2.FeSB1. Moreover, the fluorescence study illustrates that the three ferrocenecarborane conjugates differently affect the quarterly and tertiary structures as well as the polarity in the surrounding of the Trp and Tyr residues in Hb. Typically, FcSB2.mainly induces alterations of the microenvironment around the 1337Trp residue which is located on the cql32.interface of Hb. Such distinct influences are attributed to the structural features of FcSB1, FcSB2.and FcSBCO containing hydrophobic ferrocenyl and carboranyl units as well as C=O group. Screening the proteinbinding behavior can signify the potential bioactivity of such molecules and may be helpful in the future development of promising multifunctional metallodrugs.

关键词： fluorescence – UV vis absorption spectroscopy – differential pulse voltammetry – biomolecular interaction – ferrocene substituted carborane conjugates – hemoglobin

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Microwave-assisted reaction of peptide formation by amino acid with phosphate:Exploration of the most possible channels for the origin of life

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Science china chemistry 2006年第4期49卷 351-356页

作者： HU Rong1, TIAN Jinping2.& YIN Yingwu2.3 1. College of chemistry & Environment Protection Engineering, Southwest university for Nationalities, Chengdu 610041, {2. 2. TH-UNIS Insight Co., Ltd., Beijing 100085, {2. 3. Key laboratory of Bioorganic Phosphorus chemistry & chemical Biology, ministry of education, Department of chemistry, Tsinghua university, Beijing 100084, {2. College of chemistry & Environment Protection Engineering Southwest University for Nationalities Chengdu China TH-UNIS Insight Co. Ltd. Beijing China Key laboratory of Bioorganic Phosphorus {2. & chemical Biology Ministry of Education Department of Chemistry Tsinghua University Beijing China

Microwave-assisted reaction of peptide formation by amino acids with phosphate was studied. The results showed that the products were a mixture of peptides containing dipeptide, oc- tapeptides and cyclic peptides, which could be obtained in a short time. Polyphosphate was also produced synchronously by the intermolecular condensation of phosphate. The polymerization de- gree reached 99% (pyrophosphate 64%, trimetaphosphate 35%) after 2.h at 2.0℃ under microwave irradiation. The intermediates of the mixed anhydrides formed by the intermolecular condensation of phosphates and glycin were determined by ESI-MS. Peptides were also produced by the reaction of amino acids with trimetaphosphate in aqueous solution. The conversion degree of valine reached 46.5% even at room temperature. The cyclic process of peptide formation and phosphate polymeri- zation, regeneration and utilization in amino acids-phosphate system under microwave irradiation was detected and proved. Peptides could be continually formed only by inputting energy into this system. The above recycle may be the most possible process for primitive peptide formation in the origin of life.

关键词： microwave, phosphate, amino acid, peptide, origin of life.

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Fluorescence determination of platinum with dibromohydroxyphenyl-fluorone in microemulsion after separation and enrichment by sulfhydryl dextrose gel

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Rare Metals 2008年第1期27卷 101-105页

作者： LI Huizhi ZHAI Yubo ZHAI Diantang school of chemistry and {2. Engineering Jinan University Jinan 250022 China school of life Science Nanjing University Nanjing 210093 China

A new fluorescence quenching method has been developed for the determination of platinum in nonionic microemulsion *** method is based on the fact that platinum can form a stable chelate with dibromohydroxyphenyl-fluorine（DBHPF） when they are in the molar ratio of 1：2.in the nonionic microemulsion *** optimum conditions,the system,having the maximum excitation and emission wavelengths at 468 nm and 532.nm,respectively,shows constant fluorescence intensity in the pH range of 3.5-4.0 and high selectivity and sensitivity as well as low interference from foreign ***,this fluorescence intensity is a linear function of Pt（IV） concentration in the range of 0.002.1.00 μg·mL^-1 and the limit of detection is 2.90 ng·mL^-1.

关键词： analytical chemistry separation and enrichment fluorescence determination microemulsion platinum(IV)

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A simple solution route to control synthesis of Fe_3O_4 nanomaterials at low temperature and their magnetic properties

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Science china chemistry 2009年第7期52卷 916-923页

作者： LIU Yi1,2. CHEN Yun1, ZENG YuPing2.& WANG ShiLong3 1 Department of chemistry and chemical Engineering, Zaozhuang {2., Zaozhuang 2.7160, china 2.Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 2.0050, china 3 school of life Science and Technology, Tongji {2., Shanghai 2.0092. china Department of chemistry and chemical Engineering Zaozhuang University Zaozhuang China Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai China school of life Science and Technology Tongji University Shanghai China

A series of nanostructured iron compounds including cubic Fe3O4 and orthorhombic FeOOH were synthesized via a facile low temperature (in the range of 60-100℃) solution method. In the whole process, the interaction between FeCl2.4H2. and methenamine (C6H12.4) was carried out through a reflux device under different reaction conditions such as temperature, solvent, and duration. The samples were detected by XRD, TEM, SAED, physical property measurement system, and Mssbauer spectroscopy, separately. The experiments showed that magnetic mixture nanoparticles had flake and rod morphologies, and cubic Fe3O4 took on grain nanostructure. Magnetism measurements indicated that the saturated magnetization of the as-obtained magnetic mixture was lower than that of the cubic magnetite. Mssbauer spectroscopy testified the sample consisting of cubic magnetite rather than γ-Fe2.3. In addition, a possible growth mechanism of cubic magnetic nanoparticles under different conditions was discussed.

关键词： magnetic nanoparticles cubic Fe3O4 oxidation-coprecipitation method reflux magnetic properties at room temperature Mssbauer spectrum

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Topological chirality and achirality of the closed circular DNAs

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Chinese Science Bulletin 1997年第20期42卷 1705-1708页

作者： QIU Wenyuan and XIN Houwen1. Department of chemical Physics, university of Science and Technology of {2., Hefei 2.002., {2. 2. Department of chemistry, Lanzhou university, Lanzhou 730000, {2. Department of chemical Physics University of Science and Technology of China Hefei China Department of chemistry Lanzhou University I anzhou China

THE study of the relations between the topological structure and its function is one of the focus-es in modern chemistry and life science. In this note we set up a topological model to de-scribe the ccDNAs on the basis of Seifert construction in knot theory. Our model for theccDNAs helps to determine whether a structure is topologically chiral or achiral. This is one ofthe fundamental biological problems.

关键词： DNA topology knot theory topologiesl chirality topological achirality.

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Metallomics:An integrated biometal science

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Science china chemistry 2009年第12期52卷 2055-2070页

作者： GE RuiGuang1,2.& SUN HongZhe1 1 Department of chemistry and Open laboratory of {2. Biology,The university of Hong Kong,Hong Kong,china 2.laboratory of Integrative Biology,College of life Sciences,Sun Yat-Sen university,Guangzhou 510006,china Department of chemistry and Open laboratory of {2. Biology The University of Hong Kong Hong Kong China laboratory of Integrative Biology College of Life Sciences Sun Yat-Sen University Guangzhou China

Metallomics is an emerging scientific area integrating the research fields related to the understanding of the molecular mechanisms of metal-associated life processes and the entirety of metal and metalloid species within a cell or tissue type. In metallomics,metalloproteins,metalloenzymes and other metal-containing biomolecules in a biological system are referred to as metallomes,similar to genomes and proteomes in genomics and proteomics,respectively. This review discusses the concept of metallomics with a focus on analytical techniques and methods,particularly the so-called hyphenated techniques which combine a high-resolution separation technique (gel electrophoresis/laser ablation,chromatography or capillary electrophoresis) with a highly sensitive detection method such as elemental (inductively coupled plasma,ICP) or molecular (electron spray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI)) mass spectrometry,or nuclear X-ray fluorescence/absorption spectrometry. The applications of these advanced analytical methods in the identification of metallo-/phospho-/seleno-proteins,probing of relationships between structure and function of metal-loproteins,and study of clinically used metallodrugs will be selectively outlined,along with their advantages and limitations.

关键词： metallomics metallome metallodrug metalloprotein chemical speciation

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