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Inhibition of STAT3.expression by siRNA suppresses growth and induces apoptosis in laryngeal cancer cells

Acta Pharmacologica Sinica 2005年第3期26卷 377-383页

作者： Li-fang GAO~1 De-qi XU~2 Lian-ji WEN~3.Xing-yi ZHANG~4 Yue-ting SHAO~1 Xue-jian ZHAO~(1,5)1 Department of Pathophysiology,Basic School of Medicine,Jilin University,changchun 13.021,china 2 Laboratory of enteric and Sexually Transmitted Diseases,Center for Biologics Evaluation and Researeh,Food and Drug Administration,Bethesda MD,20892,USA 3.departments of ent4 thoracic and cardiovascular surgery,the second hospital of Jilin University,changchun 13.021,china Department of Pathophysiology Basic School of Medicine Jilin University Changchun China Laboratory of enteric and Sexually Transmitted Diseases Center for Biologics Evaluation and Research Food and Drug Administration Bethesda USA departments of ent Changchun China thoracic and cardiovascular surgery the Second Hospital of Jilin University Changchun China

Aim:To determine the inhibitory effect of the synthetic STAT3.siRNA on theexpression of STAT3.gene in human laryngeal cancer cell lines Hep2 and to inves-tigate the effect of STAT3.siRNA on growth and apoptosis in Hep2 ***:A pair of DNA templates coding siRNA against STAT3.mRNA was synthesized toreconstruct plasmid of pSilencer1.0-U6 ***2 cells were trans-fected with RPMI-1640 media (untreated),plasmid (empty),and STAT3.siRNA,*** blot and Western blot analysis of STAT3.and pTyr-STAT3.xpression in Hep2 cells and Western blot analysis of Bcl-2 expression in the Hep2cell was performed 72 h after ***,flow cytometry,and AO/EBassay were used for determination of cells proliferation and apoptosis in ***:pTyr-STAT3.was markedly expressed in untreated Hep2 cells andthe vector-treated Hep2 cells,whereas pTyr-STAT3.expression was significantlyreduced in STAT3.siRNA-transfected Hep2 cells,indicating that STAT3.siRNAinhibited the activity of *** of Hep2 cells with STAT3.siRNAsignificantly inhibited STAT3.expression at both mRNA and protein level in Hep2cells and the inhibition was characterized by time-dependent ***-ment of Hep2 cells with STAT3.siRNA resulted in dose-dependent growth inhibi-tion of Hep2,this significantly increased apoptotic cell rate,and decreased Bcl-2expression level in Hep2 ***3.siRNA had an effect on induction of eitherearly or late stage ***:This study demonstrates that STAT3.iRNA effectively inhibits STAT3.gene expression in Hep2 cells leading to growthsuppression and induction of apoptosis in Hep2 *** use of siRNA tech-nique may provide a novel therapeutic approach to treat laryngeal cancer andother malignant tumors expressing constitutively activated STAT3.

关键词： STAT3 RNA interference laryngeal neoplasms apoptosis

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