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Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

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Science china Chemistry 2010年第4期53卷 812-819页

作者： LI ZhiYong1, ZHANG QianYun2, ZHAO LiXia1, LI ZhenJia3, HU GuoMao3, LIN Jin-Ming2 & WANG Shan4 1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, china 2department of Chemistry, Tsinghua University, Beijing 100084, china 3Beijing Chemclin Biotech Co., Ltd., Beijing Academy of Science and Technology, Beijing 100094, china 4School of Chemistry and Chemical Engineering, xianyang normal college, xianyang 712.00, china 1. State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China2. Department of Chemistry Tsinghua University Beijing 100084 China3. Beijing Chemclin Biotech Co. Ltd. Beijing Academy of Science and Technology Beijing 100094 China4. School of Chemistry and Chemical Engineering of Xianyang Normal College Xianyang 712000 China

A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in mi- cro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H2O2)-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-250 ng mL·1. The detection limit of CEA was 0.61 ng mL·1. The coefficient of the variation was less than 7% and 13. for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation.

关键词： micro-plate magnetic chemiluminescence immunoassay carcinoembryonic antigen tumor marker

Characteristics of fMRI BOLD signal and its neurophysiological mechanism

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Progress in Natural Science:Materials International 2007年第6期17卷 630-638页

作者： Zhao Xiaohu1,2, Wu Yigen3 and Guo Shengli3(1. college of Life Sciences, Zhejiang University, Hangzhou 310058, china 2. Imaging department of Tongji Hospital of Tongji University, Shanghai 200065, china 3. college of Mathematics and physics, Nanjing University of Information Science and Technology, Nanjing 210044, china) college of Life Sciences Zhejiang University Hangzhou 310058 China Imaging department Tongji Hospital Tongji University Shanghai 200065 China college of Mathematics and physics Nanjing University of Information Science and Technology Nanjing 210044 China

The functional magnetic resonance imaging (fMRI) based on blood oxygen level dependent (BOLD) contrast has emerged as one of the most potent noninvasive tools for mapping brain function and has been widely used to explore physiological, pathological changes and mental activity in the brain. Exploring the nature and property of BOLD signal has recently attracted more attentions. Despite that great progress has been made in investigation of the characteristics and neurophysiological basis, the exact nature of BOLD signal remains unclear. In this paper we discuss the characteristics of BOLD signals, the nonlinear BOLD response to external stimuli and the relation between BOLD signals and neural electrophysiological recordings. Furthermore, we develop our new opinions regarding nonlinear BOLD response and make some perspectives on future study.

关键词： functional magnetic resonance imaging blood oxygen level dependent nonlinear response neural electrophysiological activity.

Lepton flavor violating Z-boson decays at GigaZ as a probe of supersymmetry

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Science china(physics,Mechanics & Astronomy) 2010年第11期53卷 1949-1952页

作者： YANG JinMin 1,2 1 department of physics,Henan normal University,Xinxiang 453007,china 2 Institute of Theoretical physics,Chinese Academy of Sciences,Beijing 100190,china 1. department of physics Henan Normal University Xinxiang 453007 China2. Institute of Theoretical Physics Chinese Academy of Sciences Beijing 100190 China

This paper briefly reviews the lepton flavor violating Z-decays at GigaZ as a probe of supersymmetry by focusing on Z → i j in two representative supersymmetric models: the minimal supersymmetric model without R-parity and the supersymmetric seesaw *** work concludes that under the current experimental constraints from LEP and i → j γ,these rare decays can still be enhanced to reach the sensitivity of the ***,supersymmetry can be probed via these decays at GigaZ.

关键词： Z-decay GigaZ supersymmetry

Synthesis of superheavy nuclei with ^(238)U target

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Science china(physics,Mechanics & Astronomy) 2009年第10期52卷 1482-1488页

作者： LIU ZuHua1,3 & BAO JingDong2,4 1 Key Laboratory of Beam Technology and Material Modification of Ministry of Education,Beijing normal University,Beijing 100875,china 2 department of physics,Beijing normal University,Beijing 100875,china 3 china Institute of Atomic Energy,Beijing 102413.china 4 Center of Theoretical Nuclear physics,National Laboratory of Heavy Ion Accelerator of Lanzhou,Lanzhou 730000,china Key Laboratory of Beam Technology and Material Modification of Ministry of Education Beijing Normal University Beijing 100875 China department of physics Beijing Normal University Beijing 100875 China china Institute of Atomic Energy Beijing 102413 China Center of Theoretical Nuclear physics National Laboratory of Heavy Ion Accelerator of Lanzhou Lanzhou 730000 China

The production of superheavy nuclei with Z=108-116 via hot fusion reactions of the neutron-rich projectiles with 238U target is systematically *** results show that the production cross sections of superheavy nuclei do not decrease monotonously as the atomic number Z *** cross sections of the superheavy nuclei at Z = 112 and 115 are enhanced as compared with the whole Z-trend in synthesis of the superheavy nuclei,which clearly illustrates that the reactions with large negative Q-value and shell correction are more favorable to synthesize superheavy nuclei.

关键词： superheavy nuclei fusion reaction cross section 238U target

An accurate three-dimensional potential energy surface for the He-Na_2 complex

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Science china Chemistry 2008年第6期51卷 539-544页

作者： WANG Yue,HUANG WuYing,FENG ErYin& CUI ZhiFeng department of physics,Anhui normal University,Wuhu 241000,china 1. department of physics Anhui Normal University Wuhu 241000 China

An accurate three-dimensional potential energy surface(PES) for the He-Na2 van der Waals comple was calculated at the coupled cluster singles-and-doubles with noniterative inclusion of connecte triple(CCSD(T)) level of theory.A mixed basis set,aug-cc-pVQZ for the He atom and cc-pCVQZ for th sodium atom,and an additional(3s3p2d1f) set of midbond functions were *** computed inte action energies in 819 configurations were fitted to a 96-parameter analytic potential model by leas squares *** PES has two shallow wells corresponding to the T-shaped structure and the linea configuration,which are located at 12.5a0 and 14 a0 with depths of 1.769 and 1.684 cm-1,respectivel The whole potential energy surface exhibits weak *** on the fitted PES,state-to-stat differential cross sections were calculated.

关键词： van der Waals complex potential energy surface ab initio calculation

Application of aerial remote sensing in the study on vegetation in Guangzhou,china

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Journal of Environmental Sciences 1995年第1期7卷 107-114页

作者： Chen Shijie He Shigan Yang Jielin Wang Liangping (department of Geography,Guangzhou normal college,Guanezhou 510400,china) Dept Geog Guangzhou Normal College Guangzhou China China

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ＡｐｐｌｉｃａｔｉｏｎｏｆａｅｒｉａｌｒｅｍｏｔｅｓｅｎｓｉｎｇｉｎｔｈｅｓｔｕｄｙｏｎｖｅｇｅｔａｔｉｏｎｉｎＧｕａｎｇｚｈｏｕ，ＣｈｉｎａＣｈｅｎＳｈｉｊｉｅ；ＨｅＳｈｉｇａｎ；ＹａｎｇＪｉｅｌｉｎ；ＷａｎｇＬｉａｎｇｐｉｎｇ（Ｄｅｐａｒｔｍｅｎ...

关键词： aerial remote sensing vegetation classification vegetation map ecological evaluation.

Implementation of a multiple round quantum dense coding using nuclear magnetic resonance

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Science china(physics,Mechanics & Astronomy) 2005年第6期48卷 706-715页

作者： ZHANG Jingfu1, XIE Jingyi2, WANG Chuan1, DENG Zhiwei3, LU Zhiheng4, & LONG Guilu1,5 1. Key Laboratory For Quantum Information and Measurements of MOE, department of physics, Tsinghua University, Beijing 100084,china 2. department of Materials Science and Engineering, Beijing normal University, Beijing 100875, china 3. Testing and Analytical Center, Beijing normal University, Beijing 100875,china 4. department of physics, Beijing normal University, Beijing 100875, china 5. Key Laboratory For atomic and Molecular Nanosciences, Tsinghua University, Beijing 100084,china 1. Key Laboratory For Quantum Information and Measurements of MOE Department of Physics Tsinghua University 100084 Beijing China 2. department of Materials science and Engineering Beijing Normal University 100875 Beijing China 3. Testing and Analytical Center Beijing Normal University 100875 Beijing China 4. department of physics Beijing Normal University 100875 Beijing China 5. Key Laboratory For Atomic and Molecular Nanosciences Tsinghua University 100084 Beijing China

A multiple round quantum dense coding scheme based on the quantum phase estimation algorithm is proposed and implemented in a three qubit nuclear magnetic resonance (NMR) quantum computer. Using an m + 1 qubit system, Bob can transmit one of 2m+1 messages to Alice, through manipulating only one qubit and exchanging it between Alice and Bob for m rounds. The information capacity is enhanced to m + 1 bits as compared to m bits in a classical scheme. The scheme has been demonstrated in NMR system, and the experimental results show a good agreement between theory and experiment.

关键词： quantum dense coding, multiple round, nuclear magnetic resorance

Analytical Potential Energy Function,Spectroscopic Constants and Vibrational Levels for A^1E_u^+ State of Dimer ~7Li_2

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Communications in Theoretical physics 2007年第12期48卷 1081-1087页

作者： SHI De-Heng~1 MA Heng~1 SUN Jin-Feng~(1,2)and ZHU Zun-Lue~1~1 college of physics & Information Engineering,Henan normal University,Xinxiang 453007,china~2 department of physics & Electron Science,Luoyang normal University,Luoyang 471022,china college of physics & Information Engineering Henan Normal University Xinxiang 453007 China department of physics & Electron Science Luoyang Normal University Luoyang 471022 China

The symmetry-adapted-duster configuration-interaction method is used to investigate the spectroscopicproperties of ~7Li_2(A^1∑_u^+) over the internuclear distance ranging from 2.4ao to *** complete potential energycurves are calculated at numbers of basis *** the ab initio calculated points are fitted to the analytic MurrellSorbie function and then employed to compute the spectroscopic *** comparison,the spectroscopic constantsreproduced by the potential attained at D95(3df,3pd) are found to be very close to the experiments,a^d the values (T_e,D_e,R_e,ω_e,ω_eχ_e,α_e and B_e) are of 1.732 93 eV,1.161 36 eV,0.313.27 nm,251.95 cm^(-1),1.623 cm^(-1),0.005 35 cm^(-1),and0.490 cm^(-1),*** the potential obtained at D95(3df,3pd),the totally 75 vibrational states are found whenJ=*** vibrational levels,the classical turning points and the inertial rotation constants of the first 68 vibrationalstates are calculated for the first time and compared with the available *** agreement is *** centrifugal distortion constants of the first 32 vibrational states are also reported for the first *** reasonabledissociation limit for ~7Li_2(A^1∑_u^+) is deduced using the calculated results at present.

关键词： analytic potential energy function harmonic frequency dissociation energy vibrational level classical turning point

Isomerization of B_6,B_6^- and B_6^+ clusters

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Science china Chemistry 2010年第1期53卷 202-209页

作者： WEI GongMin1,PU ZhiFeng2,ZOU Rong3,LI GuoLiang2,3 & LUO Qiong2,3 1department of physics,Capital normal University,Beijing 100048,china 2State Key Laboratory of Explosion Science and Technology Beijing Institute of Technology,Beijing 100081,china 3Center for Computational Quantum Chemistry,South china normal University,Guangzhou 510631,china 1. department of physics Capital Normal University Beijing 100048 China2. State Key Laboratory of Explosion Science and Technology Beijing Institute of Technology Beijing 100081 China3. Center for Computational Quantum Chemistry South China Normal University Guangzhou 510631 China

The interconversions between isomers with the same spin multiplicity of neutral B6 and charged B6-and B6+ clusters have been investigated at the B3LYP/6-311+G level of theory,including determination of the minimum energy pathways with transition states connecting the corresponding reactants and *** dynamic calculations,26 isomers were optimized,including 11 novel *** order to further refine the energies,single-point B3LYP/6-311+G(3df) calculations were carried out on the corresponding B3LYP/6-311+G geometries of all isomers of B6,B6-and B6+ and the corresponding isomerization transition *** stability of each isomer of B6(singlet and triplet states),B6-(doublet state) and B6+(doublet state) was analyzed from both thermodynamic and dynamic viewpoints.

关键词： B6 transition state intrinsic reaction coordinate barrier height