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Selective enrichment of hepatocytes from mouse embryonic stem cells with a culture system containing cholestatic serum

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Acta Pharmacologica Sinica 2007年第12期28卷 1931-1937页

作者： Jun MIN~(2,3),Chang-zhen SHANG~2,Ya-jin CHEN,Lei ZHANG,Lu LIU,Xiao-geng DENG,Mei YANG,Dong-ping CHEN,JunCAO,Er-wei SONG,Ji-sheng CHENDepartment of Hepatobiliary Surgery,The Second Affiliated Hospital of Sun Yat-sen university,guangzhou 510120,china Department of Hepatobiliary Surgery The Second Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510120China

Aim:There is increasing evidence indicating that embryonic stem (ES) cells arecapable of differentiating into hepatocyte-like cells in ***,it is neces-sary to improve the differentiation efficiency so as to promote the ***,we report an efficient culture system to support hepatocytedifferentiation from ES cells by utilizing cholestatic ***:One weekafter the induction of E14 mouse ES cells into hepatocytes with sodium butyrate,cholestatic serum was added into the culture system at various concentrationsand hepatocyte-like cells were induced to *** morphological andphenotypic markers of hepatocytes were characterized using light microscopy,immunocytochemistry,and RT-PCR,*** function of glycogen stor-age of the differentiated cells was detected by Periodic acid-Schiff (PAS) reaction,and the ratio of hepatic differentiation was determined by counting the albuminand PAS-positive ***:In the presence of conditional selective mediumcontaining cholestatic serum,numerous epithelial cells resembling hepatocyteswere *** RT-PCR analysis showed that undifferentiated ES cells didnot express any hepatic-specific markers;however,in the presence of sodiumbutyrate and conditional selective medium containing cholestatic serum,hepaticdifferentiation markers were *** staining showed thatthose ES-derived hepatocytes were α-fetoprotein,albumin,and cytokeratin 18positive,with the ability of storing *** determination of the hepaticdifferentiation ratio showed that the application of cholestatic serum efficientlyenriched ES-derived hepatocyte-like cells by inducing lineage differentiation andenhancing lineage ***:The conditional selective mediumcontaining cholestatic serum is optimal to selectively enrich hepatocyte-like cellsfrom mixed differentiated ES cells,which may provide a novel method to improvethe hepatic differentiation ratio of ES cells.

关键词： embryonic stem cells sodium butyrate hepatocyte cholestatic serum differentiation

集中载荷作用下具有光滑中心波纹膜片的非线性分析

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力学季刊 2003年第1期24卷 124-128页

作者：袁鸿张湘伟广东工业大学建设学院广州510640

波纹膜片是一种薄壳弹性体,由于它的参数很多,又相互制约,所以使得它的设计很复杂。在大多数位移式仪器仪表中,要求波纹膜片产生大小至少和膜片厚度是同样数量级的弹性位移,这就要求使用薄壳的几何非线性理论进行分析。大多数学者研究... 详细信息

波纹膜片是一种薄壳弹性体,由于它的参数很多,又相互制约,所以使得它的设计很复杂。在大多数位移式仪器仪表中,要求波纹膜片产生大小至少和膜片厚度是同样数量级的弹性位移,这就要求使用薄壳的几何非线性理论进行分析。大多数学者研究波纹膜片的弯曲问题,是采用扁壳理论讨论具有浅波纹的膜片。而工程实际中,经常遇到深波纹膜片,这就要求从一般壳体大挠度方程进行求解。本文采用轴对称旋转壳体的简化Re-issner方程,研究了在中心集中载荷作用下具有光滑中心波纹膜片的非线性弯曲问题。应用积分方程方法,可以获得膜片的特征关系(载荷-中心挠度关系)和应力分布。文末给出了实例计算的数值结果。

关键词：波纹膜片光滑中心非线性弯曲弹性特征圆板

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中学生元担忧与一般焦虑

心理学报 2002年第3期34卷 284-288页

作者：郑希付华南师范大学心理系广州510631

根据AdrianWells的一般焦虑的担忧模式 ,对广州市 6 36名中学生元担忧状况以及元担忧与一般焦虑的关系进行了研究。结果发现 ,高中生元担忧水平高于初中 ,显著差异。初中二年级和高中二年级的元担忧水平高于其他两个年级 ,尤其是初中 ,... 详细信息

根据AdrianWells的一般焦虑的担忧模式 ,对广州市 6 36名中学生元担忧状况以及元担忧与一般焦虑的关系进行了研究。结果发现 ,高中生元担忧水平高于初中 ,显著差异。初中二年级和高中二年级的元担忧水平高于其他两个年级 ,尤其是初中 ,显著差异。中学生元担忧的性别差异不明显。中学生元担忧各因素与焦虑均存在极显著正相关 ,考试、家庭、心理健康、外貌和身体健康

关键词：元担忧一般焦虑中学生

Variation in transboundary flow of Nujiang River and its correlation with summer monsoon under the effect of the Longitudinal Range-Gorge

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Science Bulletin 2007年第S2期 148-155页

作者： YOU WeiHong~(1+) GUO ZhiRong~(1,2) HE DaMing~3 1 Department of Atmospheric Sciences,School of Resource Environment and Earth Science,Yunnan university,Kunming 650091,china 2 Department of Atmospheric Sciences,Nanjing Information Engineering university,Nanjing 210044,china 3 Asia International Rivers center,Yunnan university,Kunming 650091,china

The relationship between the variations of the Nujiang River transboundary flow and the summer mon- soon activity under the topographical effect of the Longitudinal Range-Gorge（LRG）is *** study is based on statistical analysis of the observed river flow data,and their correlation with the reanalyzed U/V wind fields of the National centers for Environment Prediction（NCEP）and the National center for Atmospheric Research（NCAR）,and the data of the Outgoing Longwave Radiation（OLR）of the National Oceanic and Atmospheric Administration（NOAA）.It is concluded that the transboundary river flow is predominantly concentrated in the wet or rainy season（from May to October）,particularly in the summer or flood season（from June to August）.There is a clear tendency for the transboundary river flow to increase with time since 1970s,particularly apparent since *** to the topographical"east-west barrier and north-south corridor"effects of the LRG,the summer river flow variations are largely correlated with winds of the southerly and northerly at both high and low *** contrast, the river flow variations are apparently correlated wIth winds of the easterly and westerly only at the high altitudes,not at the low *** correlation between the OLR and the river flow varla- tions is also *** relationships are quantitatively described using two summer monsoon indices proposed in this *** index MI2includes both dynamical and thermodynamical effects and givee a generally good representation of the transboundary river flow variations in the LRG region of the Nujiang River *** MI2represents stronger summer monsoon activity and higher transboundary river flow,while smaller MI2suggests weaker monsoon circulation and lower water flow.

关键词： Longitudinal Range-Gorge(LRG) Nujiang River transboundary river flow summer monsoon monsoon index

CATHODE MODIFICATIONS IN CONVENTIONAL AND INVERTED SOLAR CELLS WITH HYDROPHILIC CONJUGATED POLYMER INTERLAYER

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CATHODE MODIFICATIONS IN CONVENTIONAL AND INVERTED SOLAR CEL...

第五届国际高分子化学学术讨论会

作者： Junwu Chen Yong Cao Institute of Polymer Optoelectronic Materials&Devices State Key Laboratory of Luminescent Materials &Devices South china university of Technology guangzhou 510640 china

＜正＞Recently,the special solubility of conjugated polymers in alcohol or water has drawn tremendous attention for the realization of optoelectronic devices consisting multilayer polymers as well as for the enhanced ...

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几何自动推理系统发展研究

山西广播电视大学学报 2010年第3期15卷 40-41页

作者：刘颖奇黄勇广州大学计算机科学与教育软件学院广东广州510006

通过回顾几何自动推理与几何软件的历史与研究现状,探讨了几何自动推理系统的发展,得出几何自动推理系统应向网络化发展的结论。

关键词：自动推理几何软件

Phase Evolution during Carbothermic Reduction Process of Ilmenite for TiC_xN_(1-x) Composite Powders

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稀有金属材料与工程 2011年第S3期40卷 281-285页

作者： Tang Aitao1,2, Chen Min1, Wang Jian1,2, Liu Chuanpu1, Pan Fusheng1,2, Wang Weiqing1 1 College of Materials Science and Engineering, Chongqing university,Chongqing 400045, china 2 National Engineering Research center for Magnesium Alloys, Chongqing university, Chongqing 400044, china Chongqing Univ Coll Mat Sci & Engn Chongqing 400045 Peoples R China Chongqing Univ Natl Engn Res Ctr Magnesium Alloys Chongqing 400044 Peoples R China

As cutting materials and wear-resistant materials, the composites reinforced by Ti(C,N) have very high performances. The super-high cost of these composites, however, limits apparently their wider application to industry due to use of very expensive Ti(C,N) powders. Therefore, it is very necessary to develop a new process technology with low fabrication cost. In the present work, ilmenite (FeTiO3), which is very cheap and very rich in china, was used to fabricate the Ti(C,N) powder directly. The phase evolution during carbothermic reduction process of ilmenite was investigated by XRD, TG-DSC and thermodynamic calculation. The ilmenite and graphite were mixed by high energy ball milling and then sintered in pure flowing nitrogen atmosphere. The experimental results showed that a new phase, Ti4O7, appeared at 900 ℃ and disappeared at 1100 ℃ while sintered in pure flowing nitrogen atmosphere. The phase evolution during carbothermic reduction process of ilmenite was found to be continuous as FeTiO3→Ti4O7→T3O5→TiCN. XRD results showed that the composite powders produced in the present reaction were composed of α-Fe and TiC1-xNx. The thermodynamic calculation results were agreeable with experimental results.

关键词： ilmenite TiCxN1-x composite powders thermodynamic calculation carbothermic reduction

Numerical Study on Aerodynamic Performance of CK Drone Aircraft Air Inlet in Maneuvering Flight

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Transactions of Nanjing university of Aeronautics and Astronautics 2020年第5期37卷 739-749页

作者： CAO guangzhou LI Bo LIANG Shibo TAN Hongming Key Laboratory of Advanced Technology for Small and MediumSized UAV Ministry of Industry and Information TechnologyNanjing University of Aeronautics and AstronauticsNanjing 210016P.R.China College of Energy and Power Engineering Nanjing University of Aeronautics and AstronauticsNanjing 210016P.R.China

In view of the engineering background that CK drone aircraft needs modification and upgrading to improve its maneuvering performance,numerical research and analysis of air inlet aerodynamic performance are carried ***,based on the introduction of the theoretical knowledge involved in aircraft maneuvering flight,parameters such as aircraft attitude and engine mass flow *** for the aerodynamic performance calculation of CK drone aircraft air inlet are *** analyzing the test data of WP6 engine inlet distortion simulation board,the typical indexes are extracted as the basis for evaluating the air inlet performance of CK drone ***,the aerodynamic characteristics of the inlet of CK drone aircraft under different maneuvering conditions are numerically studied,and the total pressure recovery coefficient and pressure distortion index of the outlet section are *** conclusions and suggestions are formed after the *** CK drone aircraft flies at positive angle of attack,the inlet has good aerodynamic characteristics,which can meet the requirements of engine intake during high maneuverable *** the flight of negative angle of attack,the total pressure loss and pressure distortion at the outlet section of air inlet increase sharply,which cannot guarantee the stable working of the *** the premise that the aircraft attitude is satisfied,CK drone aircraft can use three engine thrust states of"Rated","Modified rated"and"Maximum"for high maneuverable flight.

关键词： CK drone aircraft high maneuvering flight suspended nacelle inlet total pressure recovery coefficient pressure distortion index

Studies on optical dephasing of amorphous semiconductor with incoherent light

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Chinese Journal of Lasers 1993年第5期 73-79页

作者： D. J. Zhou, G. Qiu, C. H. Fu, Q. X. Li(Institute for Laser & Spectroscopy, Zhongshan university, guangzhou 510275, china)

Dephasing in a-Si0.65C0.35: H is observed by time-delayed four-wave mixing incoherent light. Results are in correspondance with consequences of disorder and localization on nonlinear optical response of the solid.

关键词： time-delayed four-wave mixing incoherent light photon echo amorphous semiconductor