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Study on interaction between microtubule associated protein tau and prion protein

science china(Life sciences) 2006年第5期49卷 473-479页

作者： HAN Jun1,ZHANG jin1,2,YAO Hailan1,3,WANG Xiaofan1,LI Feng1,CHEN Lan1,4,GAO Chen1,GAO jianmei1,NIE Kai1,ZHOU Wei1 & DONG Xiaoping11.State Key Laboratory for Infectious Diseases Prevention and Control,National Institute for Viral Disease Control and Pre-vention,Chinese Center for Disease Control and Prevention,Beijing 100052,china 2.School of Medicine,Xi’an jiao-tong university,Xi’an 710061,china 3.tong-ji medical college,hua-zhong university of science and technology,wuhan 430030,china 4.National Laboratory of medical Molecular Biology,Institute of Basic medical science,Chinese Academy of medical sciences and Peking Union medical college,Beijing 100005,china 1. State Key Laboratory for Infectious Diseases Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China 2. School of Medicine Xi’an Jiao-Tong University Xi’an 710061 China 3. tong-ji medical college Hua-Zhong University of Science and Technology Wuhan 430030 China 4. National Laboratory of medical Molecular Biology Institute of Basic Medical Science Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100005 China

Microtubule-associated protein tau is considered to play roles in many neurodegenera-tive diseases including some transmissible spongiform *** address the possible molecular linkage of prion protein(PrP) and tau,a GST-fusion segment of human tau covering the three-repeat region and various PrP segments was used in the tests of GST pull-down and *** found tau protein interacted with various style prion proteins such as native prion protein(PrPC) or protease-resistant isoform(PrPSc) .Co-localization signals of tau and PrP were found in the CHO cell tranfected with both PrP and tau *** domain of interaction with tau was located at N-terminal of PrP(residues 23 to 91) .The evidence of molecular interactions between PrP and tau protein highlights a potential role of tau in the biological function of PrP and the pathogenesis of TSEs.

关键词： tau, PrP, interaction.

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In vivo 3-photon fluorescence microscopy of white matter in mouse brain excited at the 1700 nm window

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Journal of Innovative Optical Health sciences 2024年第5期17卷 81-87页

作者： jie huang jincheng zhong Shen tong Yingxian Zhang Ping Qiu Ke Wang Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060P.R.China School of medical Information and Engineering Xuzhou Medical UniversityXuzhou 221004P.R.China

White matter,a densely packed collection of myelinated axons,plays an essential part in neural *** high spatial resolution and deep penetration,multi-photon microscopy(MPM)is promising for white matter imaging in animal models in *** third harmonic generation(THG)signal can be generated from white matter,but the bottom part of the white matter layer generates weak THG due to its high ***,we demonstrate an in vivo labeling and imaging technology,capable of visualizing the white matter layer in the mouse brain,combining°uorescence labeling with MitoTracker Red and three-photon°uorescence(3PF)microscopy excited at the 1700 nm window.3PF signals are several times higher than THG signals,resulting in deeper imaging of the white matter layer with the *** results indicate that 3PF microscopy is a promising technology for white matter imaging in the deep brain in vivo.

关键词： Three-photon microscopy white matter myelin MitoTracker Red

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Effect of Gingko Injection on the Expression of NF-κB in Pulmonary Vessels of Rats with Portal Hypertension

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生物医学工程学杂志 2005年第S1期22卷 107-108页

作者： Chao WANG Juan HAN zhong Yan LIU Zhen YANG (Department of Integrative Surgery, tongji Hospital, tong ji medical college, hua zhong university of science and technology, wuhan 430030, china) Department of Integrative Surgery Tongji Hospital Tong ji Medical College Hua Zhong University of Science and Technology Wuhan 430030 China

1 IntroductionDomestic scholars have originated the conception of'portal hypertensive vasculopathy ( PHV)', which concerns multiple organ vasculopathies in portal hypertensive patients. Portal hypertensive pulmonary vasculopathy is also a kind of internal organ vasculopathy. The transcription factor nuclear factor κB (NF-κB) regulates the expressions of genes that contribute to acute inflammatory reactions in endothelium. Few have reported about the expression of NF-κB in pulmonary vessels of rats related with portal hypertension (PH). Firstly we detected the expression of NF-κB in pulmonary vessels of rats with PH induced by biliary cirrhosis. Then through injecting of gingko (GI) into abdominal cavity, we found that NF-κB's expression was inhibited. We propose that NF-κB may play an important role in the pathogenesis of PHV and GI can hinder the improvement of PHV through inhibiting the expression of NF-κB. This outcome may provide certain theoretic basis for a novel therapy of portal hypertension.

关键词： Effect of Gingko Injection on the Expression of NF GI B in Pulmonary Vessels of Rats with Portal Hypertension

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Influences of dibutyryl cyclic adenosine monophosphate and forskolin on human sperm motility in vitro

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Asian Journal of Andrology 2003年第2期5卷 113-115页

作者： ji-Hong LIU, Yang LI, Zheng-Guo CAO, Zhang-Qun YEDepartment of Urology, tongji Hospital, tongji medical college, huazhong university of science and technology,wuhan 430030, china Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Aim: To study the influences of dibutyryl cyclic adenosine monophosphate (dbcAMP) and forskolin on human sperm motility in vitro. Methods: Semen samples, aseptically obtained by masturbation and prepared by swim-up technique from 20 fertile men, were incubated with different concentrations of dbcAMP and forskolin at 37 癈. Measurements were carried out after 10 min, 20 min, 30 min and 60 min incubation. Motility parameters were estimated by using an automatic analyzing system. Results: Treatment with dbcAMP or forskolin resulted in a significant increase in sperm motility and progressive motility. The larger the concentrations of dbcAMP or forskolin, the greater the effect appeared. The straight linear velocity and curvilinear velocity were not affected by both agents. Conclusion: dbcAMP and forskolin increase the motility and progressive motility of human sperm in vitro.

关键词： dibutyryl cyclic AMP forskolin sperm motility

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Network analysis reveals context-dependent structural complexity of social calls in serrate-legged small treefrogs

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Current Zoology 2024年第2期70卷 253-261页

作者： Ke Deng Qiao-Ling He tong-Liang Wang ji-Chao Wang jian-Guo Cui CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization&Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province Chengdu Institute of BiologyChinese Academy of SciencesChengdu 610041China university of Chinese Academy of science Beijing 100049China Ministry of Education Key Laboratory for Ecology of Tropical lslands Key Laboratory of Tropical Animal and Plant Ecology of Hainan ProvinceCollege of Life SciencesHainan Normal UniversityHaikou 571158China

Vocal communication plays an important role in survival,reproduction,and animal social *** and mammals produce com-plex vocal sequence to convey context-dependent *** are conspicuous features of the behavior of most anuran species(frogs and toads),and males usually alter their calling strategies according to ecological context to improve the attractiveness/***,very few studies have focused on the variation of vocal sequence in *** the present study,we used both conventional method and network analysis to investigate the context-dependent vocal repertoire,vocal sequence,and call network structure in serrate-legged small treefrogs Kurixalus *** found that male *** modified their vocal sequence by switching to different call types and increasing repertoire size in the presence of a competitive ***,compared with before and after the playback of advertisement calls,males emited fewer advertisement calls,but more aggressive calls,encounter calls,and compound calls during the playback *** analysis revealed that the mean degree,mean closeness,and mean betweenness of the call networks significantly decreased during the playback period,which resulted in lower *** addition,the increased proportion of one-way motifs and average path length also indicated that the connectivity of the call network decreased in competitive ***,the vocal sequence of *** did not display a clear small-world network structure,regardless of *** study presents a paradigm to apply network analysis to vocal sequence in anurans and has important implications for understanding the evolution and function of sequence patterns.

关键词： call network centrality Kurixalus odontotarsus vocal repertoire vocal sequence.

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Demethylase-assisted site-specific detection of N^(1)-methyladenosine in RNA

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Chinese Chemical Letters 2024年第5期35卷 397-401页

作者： Jun Xiong Ke-Ke Chen Neng-Bin Xie Wei Chen Wen-Xuan Shao tong-tong ji Si-Yu Yu Yu-Qi Feng Bi-Feng Yuan college of Chemistry and Molecular sciences Research Center of Public HealthRenmin Hospital of Wuhan UniversitySchool of Public HealthWuhan UniversityWuhan 430071China Department of Laboratory Medicine Zhongnan Hospital of Wuhan UniversityWuhan UniversityWuhan 430071China

The dynamic RNA modifications have been viewed as new posttranscriptional regulator in modulating gene expression as well as in a broad range of physiological processes.N^(1)-methyladenosine(m^(1)A)is one of the most prevalent modifications existing in multiple types of ***-depth investigation of the functions of m^(1)A requires the site-specific assessment of m^(1)A stoichiometry in ***,we established a demethylase-assisted method(DA-m^(1)A)for the site-specific detection and quantification of m^(1)A in RNA.N^(1)-methyl group in m^(1)A could result in the stalling of reverse transcription at m^(1)A site,thus producing the truncated *** AlkB is a demethylase that can demethylate m^(1)A to produce adenine in RNA,thus generating full-length cDNA from AlkB-treated *** of the produced amounts of full-length cDNA by quantitative real-time PCR can achieve the site-specific detection and quantification of m^(1)A in *** the DA-m^(1)A method,we examined and successfully confirmed the previously well-characterized m^(1)A sites in various types of RNAs with low false positive *** addition,we found that the level of m^(1)A was significantly decreased at the bromodomain containing 2(BRD2)mRNA position 1674 and CST telomere replication complex component 1(CTC1)mRNA position 5643 in human hepatocellular carcinoma *** results suggest that these two m^(1)A sites in mRNA may be involved in liver *** together,the DA-m^(1)A method is simple and enables the rapid,cost-effective,and site-specific detection and quantification of m^(1)A in RNA,which provides a valuable tool to decipher the functions of m^(1)A in human diseases.

关键词： N^(1)-methyladenosine RNA modification AlkB demethylase RNA epigenetics Quantitative real-time PCR Hepatocellular carcinoma

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RNF6 promotes chronic myelogenous leukemia cell proliferation and migration by stabilizing vimentin via multiple atypical ubiquitinations

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Genes & Diseases 2024年第1期11卷 87-90页

作者： Hongxia Zhang Yueya zhong Yuanming He Yujia Xu Ying Ren Haixia Zhuang tong Sun Zhigang Zhu Xinliang Mao Institute of Clinical Pharmacology Science and Technology Innovation CenterGuangzhou University of Chinese MedicineGuangzhouGuangdong 510403China Guangdong Provincial Key Laboratory of Protein Modification and Degradation School of Basic Medical SciencesGuangzhou Medical UniversityGuangzhouGuangdong 511436China Department of Pharmacology College of Pharmaceutical SciencesSoochow UniversitySuzhouJiangsu 215123China Division of Hematology&Oncology Department of GeriatricsGuangzhou First People's HospitalCollege of MedicineSouth China University of TechnologyGuangzhouGuangdong 510180China

Chronic myelogenous leukemia(CML)is a malignancy from bone marrow myeloid stem cells mainly driven by the fusion gene *** addition to BCR-ABL,other genes including RNF6 are also dysregulated in CML cells.1 RNF6,a ubiquitin ligase of the RING family,promotes various cancer cell proliferation,chemoresistance,and tumor growth in vivo by targeting various proteins for ubiquitination and degradation,including SHP1,TLE3,FOXA1,and MAD1.^(2) However,its specific mechanism in CML is not known.

关键词： ubiquitin myelogenous leukemia

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Electrostatic Encapsulation of Cobalt Ions into Crystalline Framework Derived Polymer Aerogel:Ultra-light,Pressure Resistant,Hydrophobic,Photothermal Conversion,Heat Insulation and Infrared Stealth

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Chinese Journal of Polymer science 2024年第7期42卷 946-957,I0007页

作者： Yi-Fan Kang jin-Lu Tang Ming Song Wei Wang jia-Cheng Ma Zhan-You ji tong Liu Wen-huan huang china Key Laboratory of Chemical Additives for china National Light Industry College of Chemistry and Chemical EngineeringShaanxi University of Science and TechnologyXi’an710021China college of New Energy Xi’an Shiyou UniversityXi’an710065China Xi’an Key Laboratory of Functional Supramolecular Structure and Materials College of Chemistry and Materials ScienceNorthwest UniversityXi’an710127China

A variety of electromagnetic wave absorption materials(EMWAs)have been reported,but the integration of powder materials and multifunctional devices should be investigated in-depth to adapt to practical ***,carbon-coated cobalt composites were prepared by adsorbing magnetic metal cations into an anionic crystalline framework through an electrostatic encapsulate *** reflection loss(RL_(min))of-40.49 d B and effective absorption bandwidth(EAB)of 5.36 GHz(RL34.9 dB·m^(2) for the sample *** commercial applications,Co@CN-4 was integrated into sodium carboxymethyl cellulose(CMC)aerogel to create an ultra-lightweight composite aerogel that is compressive resistant and heat-holding while also having photothermal conversion *** hydrophobic modification makes it more widely *** study provides a new strategy for EWAMs to integrate versatility and improve their application prospects.

关键词： Electrostatic incorporation Magnetic nanoparticle Co@CN composites Electromagnetic wave absorbing Metal-organic framework

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Spatial transcriptomics reveals that metabolic characteristics define the tumor immunosuppression microenvironment via iCAF transformation in oral squamous cell carcinoma

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International Journal of Oral science 2024年第1期16卷 110-121页

作者： Zheqi Liu Zhen Zhang Yu Zhang Wenkai Zhou Xu Zhang Canbang Peng tong ji Xin Zou Zhiyuan Zhang Zhenhu Ren Department of Oral and Maxillofacial-Head and Neck Oncology Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineCollege of StomatologyShanghai Jiao Tong UniversityNational Center for StomatologyNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of Stomatology&Shanghai Research Institute of StomatologyShanghaiChina Department of Oral and Maxillofacial Surgery Zhongshan HospitalFudan UniversityShanghaiChina School and Hospital of Stomatology Kunming Medical UniversityKunmingChina Center for Tumor Diagnosis&Therapy Jinshan HospitalFudan UniversityShanghaiChina Department of Pathology Jinshan HospitalFudan UniversityShanghaiChina Hainan Western Central Hospital Academician Zhang Zhiyuan Team Innovation CenterDanzhouChina

Tumor progression is closely related to tumor tissue metabolism and reshaping of the microenvironment. Oral squamous cell carcinoma (OSCC), a representative hypoxic tumor, has a heterogeneous internal metabolic environment. To clarify the relationship between different metabolic regions and the tumor immune microenvironment (TME) in OSCC, Single cell (SC) and spatial transcriptomics (ST) sequencing of OSCC tissues were performed. The proportion of TME in the ST data was obtained through SPOTlight deconvolution using SC and GSE103322 data. The metabolic activity of each spot was calculated using scMetabolism,and k-means clustering was used to classify all spots into hyper-, normal-, or hypometabolic regions. CD4T cell infiltration and TGF-βexpression is higher in the hypermetabolic regions than in the others. Through CellPhoneDB and NicheNet cell-cell communication analysis, it was found that in the hypermetabolic region, fibroblasts can utilize the lactate produced by glycolysis of epithelial cells to transform into inflammatory cancer-associated fibroblasts (iCAFs), and the increased expression of HIF1A in iCAFs promotes the transcriptional expression of CXCL12. The secretion of CXCL12 recruits regulatory T cells (Tregs), leading to Treg infiltration and increased TGF-β secretion in the microenvironment and promotes the formation of a tumor immunosuppressive microenvironment. This study delineates the coordinate work axis of epithelial cells-iCAFs-Tregs in OSCC using SC, ST and TCGA bulk data, and highlights potential targets for therapy.

关键词： CXCL12 microenvironment squamous

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Possible Role of Cellular Polyamine Metabolism in Neuronal Apoptosis

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Current medical science 2024年第2期44卷 281-290页

作者： Xin-tong ji Wen-lei YU Meng-jia jiN Lin-jie LU Hong-ping YIN huan-huan WANG School of Basic medical sciences Affiliated Hospital of Hangzhou Normal UniversityHangzhou Normal UniversityHangzhou 311121China School of Medicine Chongqing UniversityChongqing 400030China Department of Stomatology Huzhou Wuxing District People's HospitalHuzhou Wuxing District Maternal and Child Health HospitalHuzhou 313008China School of Pharmacy Zhejiang UniversityHangzhou 310030China Department of Stomatology Haining Hospital of Traditional Chinese MedicineJiaxing 314400China

Recent studies have shown that cellular levels of polyamines(PAs)are significantly altered in neurodegenerative *** from in vivo animal and in vitro cell experiments suggests that the cellular levels of various PAs may play important roles in the central nervous system through the regulation of oxidative stress,mitochondrial metabolism,cellular immunity,and ion channel *** of PA metabolism related enzymes also contributes to neuronal injury and cognitive impairment in many neurodegenerative ***,in the current work,evidence was collected to determine the possible associations between cellular levels of PAs,and related enzymes and the development of several neurodegenerative diseases,which could provide a new idea for the treatment of neurodegenerative diseases in the future.

关键词： polyamine apoptosis mitochondria neurodegenerative disease

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