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Sequential Phosphorylation of Nedd1 by Cdk1 and Plk1 is required for γTuRC targeting to centrosome

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生物物理学报 2009年第S1期25卷 245-246页

作者： Xiaoyan Zhang, Qiang Chen, Qing Jiang and Chuanmao Zhang The MOE key laboratory of Cell Proliferation and Differentiation and the {2. key laboratory of Bio-membrane and membrane Bio-engineering, College of Life Sciences, Peking University, beijing 100871, China

Nedd1 (neural precursor cell expressed, developmentally down-regulated gene 1) is an evolutionarily conserved protein that has previously been characterized as a new member

关键词： Sequential Phosphorylation of Nedd1 by Cdk1 and Plk1 is required for TuRC targeting to centrosome

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Effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of bone marrow stromal cells and on the adipogenic trans-differentiation of osteoblasts

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Progress in Natural Science:Materials International 2005年第11期15卷 993-999页

作者： LI Xianghui, ZHANG Jinchao, SUI Senfang and YANG Mengsu (state key {2. of{3., Department of Biological Sciences and biotechnology, Tsinghua University, beijing 100084, China Department of Chemistry, College of Chemistry & Environmental Science, Hebei University, Baoding 071002. China Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China) state key {2. of{3. Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Department of Chemistry College of Chemistry and Environmental Science Hebei University Baoding 071002 China Department of Biology and Chemistry City University of Hong Kong Hong Kong Hong Kong

The 3.(4,5-dimethylthiazol-2.yl)-2.5-diphenyl tetrazolium bromide (MTT), alkaline phosphatase (ALP) activity and oil red O assays were used to examine the effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells (MSCs) and the adipogenic trans-differentiation of primary mouse osteoblasts. The results indicated that daidzein, genistein and glycitein at concentrations from 1×10-8 mol/L to 1×10-5mol/L promoted the proliferation of MSCs and osteoblasts; genistein, daidzein and glycitein promoted osteogenic differentiation and inhibited adipogenic differentiation of MSCs, and inhibited adipocytic transdifferentiation of osteoblasts at appropriate concentrations as 17β-estradiol. It suggests that genistein, daidzein and glycitein regulate a dual differentiational process of MSCs into the osteogenic and adipogenic lineages, and trans-differentiational process of primary osteoblasts into the adipocyte lineages, causing a lineage shift toward osteoblast. Protective effects of them on bone may be mediated by a reversal of adipogenesis which may promote the proliferation, differentiation and mineralization of osteoblasts, and make adipocytes secrete less cytokines which may promote osteoclast formation and activation. In addition, the results also indicated that genistein, daidzein and glycitein may be helpful in preventing the development of steroid induced osteonecrosis.

关键词： genistein daidzein glycitein bone marrow stromal cells osteoblasts osteogenic differentiation adipogenic differentiation adipogenic trans-differentiation.

Cloning and expression analysis of a dirigent protein gene from the resurrection plant Boea hygrometrica

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Progress in Natural Science:Materials International 2009年第3期19卷 347-352页

作者： Renhua Wu, Lili Wang, Zhi Wang, Haihong Shang, Xia Liu, Yan Zhu, Dongdong Qi, Xin Deng * key laboratory of Photosynthesis and Environmental Molecular Physiology, {2. of Botany,{3. academy of Sciences, beijing 100093. China key laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China

Resurrection plants are tolerant to extreme dehydration and are useful model systems to study genes that play a role in drought tolerance. A gene fragment encoding a dirigent protein that is predicted to function in lignin biosynthesis was identified from leaves of the resurrection plant Boea hygrometrica via cDNA microarray screening. A cDNA, designated BhDIR1, containing the complete predicted open reading frame, was obtained by 50-RACE. BhDIR1 transcripts were found to be accumulated in response to changes in plant dehydration status, exogenously applied phytohormones and signaling molecules, and temperature stresses. BhDIR1 encodes a protein of 199 amino acids, which shows 2.-40% similarity to dirigent proteins reported from other plants. BhDIR1 is predicted to contain a cleavable signal peptide at the N-terminal, and its plasma membrane/cell wall localization was confirmed using a GFP fusion protein assay. Consistent with this discovery, the acid-soluble lignin content decreased in dehydrated B. hygrometrica leaves. Taken together, our results indicate a protective role for a dirigent protein in response to drought stress by changing the physical characters of lignin which in turn is predicted to affect the mechanical strength and flexibility of the plant cell wall.

关键词： Resurrection plant Dirigent protein Drought Lignin Cell wall

A Genome-wide Functional Characterization of Arabidopsis Regulatory Calcium Sensors in Pollen Tubes

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Journal of Integrative Plant Biology 2009年第8期51卷 751-761页

作者： Liming Zhou1,2. Ying Fu1,2.and Zhenbiao Yang2.3.(1state {2. laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University,{3. 100193. China 2.hina Agricultural University (CAU)-University of California, Riverside (UCR) Joint Center for Biological Sciences and biotechnology, College of Biological Sciences, China Agricultural University,{3. 100193. China 3.enter for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92.2., USA) state key {2. of Plant Physiology and Biochemistry Department of Plant Sciences College of Biological Sciences China Agricultural University Beijing 100193 China China Agricultural University (CAU)-University of California Riverside (UCR) Joint Center for Biological Sciences and Biotechnology College of Biological Sciences China Agricultural University Beijing 100193 China Center for Plant Cell Biology and Department of Botany and Plant Sciences University of California Riverside CA 92521 USA)

Calcium, an ubiquitous second messenger, plays an essential and versatile role in cellular signaling. The diverse function of calcium signals is achieved by an excess of calcium sensors. Plants possess large numbers of calcium sensors, most of which have not been functionally characterized. To identify physiologically relevant calcium sensors in a specific cell type, we conducted a genome-wide functional survey in pollen tubes, for which spatiotemporal calcium signals are well-characterized and required for polarized tip growth. *** members of calrnodulin （CAM）, CaM-like （CML）, calcium-dependent protein kinase （CDPK） and calcineurin B-like protein （CBL） families were tagged with green fluorescence protein （GFP） and their localization patterns and overexpression phenotypes were characterized in tobacco pollen tubes. We found that several fusion proteins showed distinct overexpression phenotypes and subcellular localization patterns. CDPK2.-GFP was localized to the vegetative nucleus and the generative cell/sperms. CDPK3.-GFP caused severe growth depolarization. CBL2.GFP and CBL3.GFP exhibited dynamic patterns of subcellular localization, including several endo{2. compartments, the apical plasma {2. （PM）, and cytoskeleton-like structures in pollen tubes. Their overexpression also inhibited pollen tube elongation and induced growth depolarization. These putative calcium sensors are excellent candidates for the calcium sensors responsible for the regulation of calcium homeostasis and calciumdependent tip growth and growth oscillation in pollen tubes.

关键词： Arabidopsis calcium sensor calcium signaling Rho-related GTPase from plants subcellular localization tip growth

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In vitro and in vivo evaluation of a novel oral insulin formulation

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Acta Pharmacologica Sinica 2006年第10期27卷 1382-1388页

作者： Er-li MA Hong MA Zheng LIU Chang-xue ZHENG Ming-xing DUAN~2.state key {2. of Bio-{3. and biotechnology,Department of Biological Sciences and biotechnology,Tsinghua University beijing 100084,China state key {2. of Bio-{3. and biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Aim:To develop a stable self-emulsifying formulation for oral delivery of ***:Caco-2.cell line and diabetic beagles were used as in vitro and in vivomodels to study the absorption mechanism and the hypoglycemic efficacy of *** addition,various physicochemical parameters of the formulationsuch as droplet size,insulin encapsulation efficiency and stability were ***:This formulation enabled changes in barrier properties of Caco-2.onolayers,as referred by transepithelial electrical resistance(TEER)and appar-ent permeability coefficients(Papp)of the paracellular marker ranitidine(2.-foldgreater than control)but not transcellular marker propranolol,suggesting that theopening of tight junctions was *** diabetic beagle dogs,the bioavailabilityof this formulation was up to 15.2. at a dose of 2.5 IU/kg in comparison with thehypoglycemic effect of native insulin(0.5 IU/kg)delivered by ***:This formulation,recently approved by the China stateFood and Drug Administration to enter clinical trials,was stable,degradation-protected and absorption-enhanced,and provided a promising formulation fororal insulin delivery.

关键词： insulin oral delivery Caco-2 diabetic beagles

Anticancer activity of sodium caffeate and its mechanism

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Acta Pharmacologica Sinica 2005年第10期26卷 1248-1252页

作者： Feng XU~(2.3.4), Sheng-hua ZHANG~3. Rong-guang SHAO~3. Yong-su ZHEN~3.~2.Department of Pharmacology Zhujiang Hospital, Southern Medical University Guangzhou 5102.0 ~3.Department of Ontology Medicinal biotechnology, chinese {2. of Medical Sciences and Peking Union Medical College, {3. 100050, China DepartmentofPharmacology ZhujiangHospitalSouthernMedicalUniversityGuangzhou510280 DepartmentofOncology InstituteofMedicinalBioteehnologvChineseAcademyofMedicalSciencesandPekingUnionMedicalCollegeBeijing100050China

Aim: To study the anticancer activity of sodium caffeate （SC）. Methods: A nucleoside transport assay was used to analyze the inhibitory effects of SC on nucleoside rescue. The MTT assay was used to measure cell proliferation. Flow cytometrywas used to measure the apoptosis of BEC-7402.induced by SC and the cell cycledistribution change. Western blotting analysis was employed to investigate Bcl2. caspase and Bax expression. Intracellular Ca2.and mitochondrial membranepotential were determined by flow cytometry. In vivo anti-tumor activity wasmeasured using a tumor transplantation model in mice. Results: SC inhibited thenucleoside transport of BEL-7402.cells with an IC50of 1.02.mg/mL. SC inhibitedtumor cell proliferation with an IC50between 100 μg/mL and 2.0μg/mL. SC induced BEL-7402.cell apoptosis in a time-and dose-dependent manner, which wasinduced by arresting cells in S phase. The in vivo study showed that tumorgrowth was inhibited in a dose-dependent manner. Activated caspase-3.and Baxexpression were up-regulated after treatment with SC, while Bcl-2.expression wasdown-regulated. Intracellular Ca2.was increased while mitochondrial membranepotential was decreased by SC. Conclusion: SC is a new anticancer agent withpromising potential.

关键词： caffeic acid anticancer activity cell division cell apoptosis cell cycle phytogenic antineoplastic agent flow cytometry Western blotting

Drosophila miR-5 suppresses Hedgehog signaling by directly targeting Smoothened

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Drosophila miR-5 suppresses Hedgehog signaling by directly t...

Blockade of paeoniflorin on sodium current in mouse hippocampal CA1 neurons

2.12.国发育生物学大会

作者： Long-Fei Wu,Lei Gao,Xiao-MengHou,Qing-Hai Zhang,Shen Li,Yong-Fei Yang~* Xin-Hua Lin~* state key {2. of{3. and membrane biotechnology,and key {2. of Stem Cell and Developmental Biology,institute of zoology,chineseacademy of Sciences,{12.,{13.,China

Hedgehog(Hh) signaling plays many important roles in developmental processes ***(Smo) is an important signal transducer in the Hh pathway,andits expression is tightly regulated by several different post-transcriptional ***,whether microRNAs(miRNAs) involved in Smo regulation is still ***, we performed a genetic screen and identified miR-5,which can directly bind tothe 3.UTR of ***,we showed that reduction of Smo was not dueto the feedback effect of Costal2.Cos2. or Fused(Fu).Furthermore,we determinedmiR-5 had no effect on Notch or Dpp signaling pathways. ConclusionrTaken together,theseresults indicate that miR-5 can specifically suppress Hh signaling by directly targetingSmoin Drosophila.

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Acta Pharmacologica Sinica 2003年第12期24卷 74-78页

作者： ZHANG Guang-Qin, HAO Xue-Mei, CHEN Shi-Zhong~1, ZHOU Pei-Ai, CHENG He-Ping, WU Cai-Hong~2.state key {2. of{3. and membrane biotechnology, College of Life Sciences, Peking University, beijing 100871 ~1Department of Traditional chinese Medicine, School of Pharmaceutical Science, Peking University, beijing 100083. China College of Life Sciences Peking University State Key Laboratory of Biomembrane and Membrane Biotechnology 北京 100871 Department of Traditional chinese Medicine School of Pharmaceutical Science Peking University 北京 100083

AIM: To study the blockade of paeoniflorin (Pae) on INain the acutely isolated hippocampus neurons of mice. METHODS: The whole-cell patch clamp technique was used. RESULTS: Pae inhibited INain frequency-dependent and concentration-dependent manners, with an IC50of 2.1μmol/L. Pae 0.3.mmol/L shifted the activation potential of the maximal INafrom -40 mV to -3. mV, shifted the steady-{2. activation and inactivation curves toward more positive and negative potentials by 10.8 mV, and 18.2.mV, respectively, and postponed the recovery of INainactivation {2. from (4.2.0.7) ms to (9.8±1.2. ms. CONCLUSION: Pae inhibited INain mouse hippocampus neurons.

关键词： hippocampus patch-clamp techniques paeoniflorin sodium channels

CONTROL OF POLYMER PARTICLE SIZE USING POROUS GLASS membrane EMULSIFICATION A REVIEW

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China Particuology 2003年第3期1卷 105-114页

作者： Guanghui Ma state key {2. of Biochemical Engineering,{3. of Process Engineering, chinese academy of Sciences, beijing 100080, P. R. China Tel: 0086-10-82.2.072. ghma@*** state key {2. of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing 100080 P. R. China

Much attention has in recent years been paid to fine applications of polymer particles, e.g., carrier for enzyme, separation media for protein, DNA and cell, and carrier for drug in Drug Delivery System (DDS). Control of polymer particle size is especially important in such fine applications. For instance, when the particles are used as a carrier of anti-cancer agents, the locations of particles containing anti-cancer agents also depend on the size of the particles. In this paper, various techniques of controlling polymer particle size are described, with emphasis on Shirasu Porous Glass (SPG) membrane emulsification, as carried out in our research group.

关键词： particles polymerization emulsification Shirasu porous glass membrane microcapsule

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