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Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica(Phaeophyta)

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Acta Oceanologica Sinica 2012年第6期31卷 139-148页

作者： XUAN Jinsong 1,4,FENG Yanbin 1,WENG Manli 1,ZHAO Ge 1,SHI Jinfeng 1,YAO Jianting 2.WANG Xiuliang 2.GUO Baotai 3.QIAO Lixian 3.DUAN Delin 2.WANG Bin 1 1 The {2. key laboratory of Plant Genomics,{3. of Genetics and Developmental Biology,chinese academy of Sciences,beijing 100101,China 2.The {2. key laboratory of Marine Biology,{3. of Oceanology,chinese academy of Sciences,Qingdao 2.6071,China 3.College of Life Sciences,Qingdao Agricultural University,Qingdao 2.6109,China 4 Department of Biological Science and Engineering,School of Chemical and Biological Engineering,University of Science and Technology beijing,beijing 100083.China 1. The state key {2. of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China 4. Department of Biological Science and Engineering School of Chemical and Biological Engineering University of Science and Technology Beijing Beijing 100083 China 2. The state key {2. of Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China 3. College of Life Sciences Qingdao Agricultural University Qingdao 266109 China

A high quality cDNA library was constructed from the brown alga Laminaria japonica,with the titer of 1.2.10 5 pfu/*** average insert size of the cDNA library is about 1.6 *** the cDNA library,591 cDNA clones were randomly selected and *** a result,574 EST(expressed sequence tag) sequences were *** of the 574 ESTs were submitted to the dbEST database section of GenBank with the accession numbers from CX942.2. to *** cDNA library was screened with a α-3. p labeled 453.bp T P S gene probe,which is a partial sequence yielded from Porphyra *** positive cDNA clones were screened and the sequencing data showed that these four cDNA clones covered majority of *** TPS cDNA *** PCR amplification,sequencing and assembling,the entire ORF(open reading frame) sequence of the T P S gene was obtained,which was named *** encodes a protein containing 908 amino acids with a calculated molecular mass of 101 674 *** LjTPS gene was successfully expressed in *** and *** LjTPS gene has potential application both in plant breeding to stress tolerance and in deciphering the T P S gene function and mechanism to stress tolerance.

关键词： cDNA library comparative study expressed sequence tag(EST) analysis Laminaria japonica trehalose-6-phosphate synthase(TPS) T P S gene

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Spectroscopic evidence for triplet excitation energy transfer among carotenoids in the LH2.complex from photosynthetic bacterium Rhodopseudomonas palustris

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Science China Chemistry 2004年第1期47卷 80-90页

作者： FENG Juan, WANG Qian, ZHANG Xujia, HUANG Youguo, Al Xicheng, ZHANG Xingkang & ZHANG Jianpingstate key {2. for Structural Chemistry of Unstable and Stable Species,{3. of Chemistry, chinese academy of Sciences, beijing 100080, China National {2. of Biomacromolecules,{3. of Biophysics, chinese academy of Sciences, beijing 100101, China 1. state key {2. for Structural Chemistry of Unstable and Stable Species{3. of Chemistry Chinese Academy of Sciences 100080 Beijing China 2. National laboratory of Biomacromolecules institute of Biophysics Chinese Academy of Sciences 100101 Beijing China

The LH2.complex from Rhodopsudomonas (Rps.) palustris is unique in the heterogeneous carotenoid compositions. The dynamics of triplet excited state Carotenoids (3.ar* has been investigated by means of sub-microsecond time-resolved absorption spectroscopy both at physiological temperature (2.5 K) and at cryogenic temperature (77K). Broad and asymmetric T n ←T 1 transient absorption was observed at room temperature following the photo-excitation of Car at 53. nm, which suggests the contribution from various carotenoid compositions having different numbers of conjugated C=C double bonds (Nc=c). The triplet absorption bands of different carotenoids, which superimposed at room temperature, could be clearly distinguished upon decreasing the temperature down to 77 K. At room temperature the shorter-wavelength side of the main Tn04T1 absorption band decayed rapidly to reach a spectral equilibration with a characteristic time constant of ∽1 μs, the same spectral dynamics, however, was not observed at 77 K. The aforementioned spectral dynamics can be explained in terms of the triplet-excitation transfer among heterogeneous carotenoid compositions. Global spectral analysis was applied to the time-resolved spectra at room temperature, which revealed two spectral components peaked at 545 and 565 nm, and assignable to the Tn04 T1 absorption of Cars with Nc=c=11 and Nc=c=13. respectively. Surprisingly, the decay time constant of a shorter-conjugated Car, i.e. 0.72.?s (aerobic) and 1.3. ?s (anaerobic), is smaller than that of a longer-conjugated Car, i.e. 2.12.us (aerobic) and 3.75 ?s (anaerobic), which is contradictory to the general rule of carotenoids and relative polyenes. The results are explained in terms of triplet-excitation transfer among different types of Cars. It is postulated that two Cars with different conjugation lengths coexist in an α, β-subunit in the LH2.complex.

关键词： purple photosynthetic bacteria, carotenoid, excited-state, time-resolved spectroscopy.

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Seasonal patterns of spatial differentiation of landcover change in China

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chinese Science Bulletin 1999年第4期44卷 362-364页

作者： CHEN Yufengstate key {2. of Resource and Environment Information System ,{3. of Geography, chinese academy of Sciences, beijing 100101, China state key {2. of Resource and Environment Information System Institute of Geography Chinese Academy of Sciences Beijing China

Using the 1°×1° NOAA AVHRR NDVI data set in China, an index on the spatial different gradient of landcover is created. Then based on the index and supported by the geographical information system, it is revealed through principal component and factor analyses methods that there exist four types of seasonal patterns of spatial differentiation of landcover change in China, i.e. November to next March type, June to September type, May or October type and April type.

关键词： landcover spatial differentiation seasonal pattern.

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The role of reservoir mediums in natural oil cracking:Preliminary experimental results in a confined system

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chinese Science Bulletin 2010年第33期55卷 3787-3793页

作者： XIAO QiLin1,2.SUN YongGe1,3.& ZHANG YongDong1,2.1 state {2. laboratory of Organic Geochemistry,Guangzhou{3. of Geochemistry,chinese academy of Sciences,Guangzhou 510640,China 2.Graduate Schools,chinese academy of Sciences,beijing 100049,China 3.3. of Environmental and Biogeochemistry(eBig),Zhejiang University,Hangzhou 3.002.,China state key {2. of Organic Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China Graduate Schools Chinese Academy of Sciences Beijing 100049 China institute of Environmental and Biogeochemistry (eBig) Zhejiang University Hangzhou 310027 China

Large amounts of data regarding the influence of temperature and pressure on the thermal stability of crude oil have been pub-lished;however,the role of reservoir mediums has received little *** involving oil cracking in the presence of montmorillonite,illite,calcite,quartz and water were conducted in closed gold tubes to investigate the effects of these reservoir mediums on oil *** was done by screening variations in the chemical and stable carbon isotopic components of nC10+ and gasoline-range hydrocarbons(nC8-)present in various *** indicated that reservoir mediums have an active role in oil cracking under experimental *** concentrations of nC10+ in the cracked residues progressively decreased in systems containing oil+water+illite,oil+water+montmorillonite,oil+water,oil+water+quartz and oil+water+*** comparison with the system containing oil+water,our results indicated a retardation effect for oil cracking in systems in the presence of illite and montmorillonite,and an acceleration effect on oil destruction in systems in the presence of calcite and ***10+ became increasingly depleted in 13. in systems with oil+water+illite,oil+water+calcite,oil+water+montmorillonite,oil+water+ quartz and oil+*** obvious correlation was observed between concentrations and stable carbon isotopic components of nC6-nC8 and nC10+ in the individual *** discrepancies in chemical and stable carbon isotopic components of nC6-nC8 and nC10+ in the pyrolyzed residues highlighted the important role of reservoir mediums to control carbon-carbon cleavage of nC10+ and then the isomerization,cyclolization and aromatization reactions;as well as governing the occurrence and thermal destruction of nC6-nC8 under experimental *** research may have critical implications in reconstructing chemical kinetic models for natural oil cracking.

关键词：裂解作用实验条件密闭系统原油裂解水库介质天然同位素组成

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Synchronous estimation of DTM and fractional vegetation cover in forested area from airborne LIDAR height and intensity data

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Science China(Technological Sciences) 2008年第S2期51卷 176-187页

作者： BAO YunFei1,2.CAO ChunXiang1,ZHANG Hao1,CHEN ErXue3.HE QiSheng1,2.HUANG HuaBing1,LI ZengYuan3.LI XiaoWen1,4 & GONG Peng1 1 {2. key laboratory of Remote Sensing Science,jointly sponsored by the{3. for Remote Sensing Applications of chinese academy of Sciences and beijing Normal University,beijing 100101,China 2.Graduate University of the chinese academy of Sciences,beijing 100049,China 3.3. of Forest Resource Information Technique,the chinese academy of Forestry,beijing 100091,China 4 Research Center for Remote Sensing and GIS,Department of Geography and beijing key laboratory for Remote Sensing of Environment and Digital Sites,beijing Normal University,beijing 100875,China state key {2. of Remote Sensing Science jointly sponsored by the Institute for Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University Beijing 100101 China Graduate University of the chinese academy of Sciences Beijing 100049 China institute of Forest Resource Information Technique the Chinese Academy of Forestry Beijing 100091 China Research Center for Remote Sensing and GIS Department of Geography and Beijing Key Laboratory for Remote Sensing of Environment and Digital Sites Beijing Normal University Beijing 100875 China

We proposed a method to separate ground points and vegetation points from discrete return,small footprint airborne laser scanner data,called skewness change *** method,which makes use of intensity of laser scanner data,is especially applicable in steep,and forested *** does not take slope of forested area into account,while other algorithms consider the change of slope in steep forested *** ground points and vegetation points can be used to estimate digital terrain model(DTM) and fractional vegetation cover,respectively.A few vegetation points which were classified into the ground points were removed as noise before the generation of *** method was tested in a test area of 10000 square meters.A LiteMapper -5600 laser system was used and a flight was carried out over a ground of 700―800 *** this tested area,a total number of 1546 field measurement ground points were measured with a total station TOPCON GTS-602.and TOPCON GTS -7002.for validation of DTM and the mean error value is -18.5 cm and the RMSE(root mean square error) is ±2..9 cm.A data trap sizes of 4m in diameter from airborne laser scanner data was selected to compute vegetation fraction *** of fractional vegetation cover was carried out using 15 hemispherical photographs,which are georeferenced to centimeter accuracy by differential *** gap fraction was computed over a range of zenith angles 10° using the gap light analyzer(GLA) from each hemispherical *** R2.for the regression of fractional vegetation cover from these ALS data and the respective field measurements is *** this study presents a method for synchronous estimation of DTM and fractional vegetation cover in forested area from airborne LIDAR height and intensity data.

关键词： LIDAR intensity skewness synchronous DTM fractional vegetation cover

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Spectral response of rice(Oryza sativa L.) leaves to Fe^(2.) stress

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Science China(Life Sciences) 2009年第8期52卷 747-753页

作者： CHI GuangYu1,2.CHEN Xin1,SHI Yi1 & LIU XinHui3.1 key {2. of Terrestrial Ecological Process,institute of Applied Ecology,{3. academy of Sciences,Shenyang 110016,China 2.Graduate School of {2. academy of Sciences,beijing 100049,China 3.state key Joint {2. of Environmental Simulation and Pollution Control,School of Environment,beijing Normal University,beijing 100875,China key laboratory of Terrestrial Ecological Process Institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 China Graduate School of chinese academy of Sciences Beijing 100049 China state key Joint {2. of Environmental Simulation and Pollution Control School of Environment Beijing Normal University Beijing 100875 China

In the management of lake eutrophication,the regulation effect of Fe is considered,in addition to the controlling nitrogen-and phosphorus *** on the "Fe hypothesis",this paper tentatively applied plant spectral response to the remote sensing early-warning mechanism of lake eutrophication.A laboratory water culture experiment with rice(Oryza sativa L.) was conducted to study Fe uptake by plants and the chlorophyll concentration and visible-near infrared spectrum of vegetable leaves as well as their interrelations under Fe2. *** spectral indices,i.e.,A1(integral value of the changes of spectral reflectivity in the range 460―670 nm under Fe2. stress),A2.integral value of the changes of spectral reflectivity in the range of 760―1000 nm under Fe2. stress) and S(blue-shift range of red edge curve under Fe2. stress),were used to establish quantitative models about the relationships between the rice leaf spectrum and Fe2. *** the increase of Fe2. in a culture solution,the Fe content in rice plants increased,while the chlorophyll concentration in vegetative leaves *** spectral reflectivity of vegetable leaves increased in the visible light band but decreased in the near infrared band,and the blue-shift range of the red edge curve *** indices A1,A2.and S all had significant correlations with the Fe content in rice leaves,the correlation coefficient being respectively 0.951(P 2.> 0.96) and predict the Fe level in rice leaves.

关键词： rice(Oryza sativa L.) spectrum Fe2. lake eutrophication

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A prototype auto-human support system for spatial analysis

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Progress in Natural Science:Materials International 2006年第9期16卷 954-966页

作者： LI Lianfa and WANG Jinfeng (state key {2. of Resources & Environmental Information System,{3. of Geographical Sciences & Natural Resources Research, chinese academy of Sciences, beijing 100101, China) [a]state key {2. of Resources & Environmental Information System Institute of Geographical Sciences & Natural Resources Research Chinese Academy Sciences Beijing China

Spatial analysis is a multidisciplinary field that involves multiple influential factors, variation and uncertainty, and modeling of geospatial data is a complex procedure affected by spatial context, mechanism and assumptions. In order to make spatial modeling easier, some scholars have suggested a lot of knowledge from exploratory data analysis (EDA), specification of the model, fitness and diagnosis of the model, to interpretation of the model. Also an amount of software has improved some functionalities of spatial analysis, e. g. EDA by the dynamic link (GeoDa) and robust statistical calculation (R). However, there are few programs for spatial analysis that can automatically deal with unstructured declarative issues and uncertainty in machine modeling using the domain knowledge. Under this context, this paper suggests a prototype support system for spatial analysis that can automatically use experience and knowledge from the experts to deal with complexity and uncertainty in modeling. The knowledge base component, as the major contribution of the system, in support of the expert system shell, codes and stores declarative modeling knowledge, e.g. spatial context, mechanisms and prior knowledge to deal with declarative issues during the modeling procedure. With the open architecture, the system integrates functionalities of other components, e.g. GIS’ visualization, DBMS, and robust calculation in an interactive environment. An application case of spatial sampling, design and implementation of spatial modeling with such a system is demonstrated.

关键词： spatial analysis automatic support system modeling of spatial data artificial inference for modeling.

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The excitation energy transfer between β-Car and Chla molecules in PS Ⅱ core antenna complexes CP43.and CP47

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chinese Science Bulletin 2000年第17期45卷 1579-1583页

作者： SHAN Jixiu, WANG Jushuo, LI Liangbi,ZHAO Nanming & KUANG Tingyun1. laboratory of Photosynthesis Basic Research, institute of Botany,{2. {3. of Sciences, beijing 100093. China 2. Department of Biological Sciences and biotechnology, state key laboratory of biomembrane and membrane biotechnology, Tsinghua University, beijing 100084, ChinaCorrespondence should be addressed to Kuang Tingyun laboratory of Photosynthesis Basic Research Institute of Botany Chinese Academy of Sciences Beijing China Department of Biological Sciences and biotechnology State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China

The excitation energy transfer efficiency between p-Car and Chla molecules in purified CP43.and CP47 was calculated by comparing absorption and fluorescence excitation after normalization at 550 nm, CP43.had an energy transfer efficiency of 2..1% while the CP47 had an energy transfer efficiency of 62.8%, proving that excitation energy was transferred between p-Car and Chla molecules in CP43.and CP47 at normal conditions. The excitation energy transfer between p-Car and Chla molecules in CP43.and CP47 may occur through the "Dexter" mechanism and the distance between these two kinds of pigments should be less than 1 nm. In addition, the results were also used to discuss the conformational relationship between p-Car and Chla molecules in CP43.and CP47.

关键词： CP43 CP47 β-Car Chla excitation energy transfer.

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Propagation characteristics of converted refracted wave and its application in static correction of converted wave

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Science China Earth Sciences 2008年第S2期51卷 226-232页

作者： LIU Yang1,2.& WEI XiuCheng3.1 state {2. laboratory of Petroleum Resource and Prospecting,China University of Petroleum,{3. 102.49,China 2.CNPC Geophysical Exploration {2. laboratory,China University of Petroleum,{3. 102.49,China 3.Exploration and Production Research institute,SINOPEC,{3. 100083.China China Univ Petr State Key Lab Petr Resource & Prospecting Beijing 102249 Peoples R China China Univ Petr CNPC Geophys Explorat Key Lab Beijing 102249 Peoples R China SINOPEC Explorat & Prod Res Inst Beijing 100083 Peoples R China

Three-component seismic exploration through P-wave source and three-component geophone is an effective technique used in complicated reservoir exploration. In three-component seismic exploration data processing,one of the difficulties is static correction of converted wave. This paper analyzes propagation characteristics of non-converted and converted refracted waves,and discovers a favor-able condition for the formation of converted refracted wave,i.e. the velocity of overlaying medium S wave is much lower than that of underlying medium S wave. In addition,the paper proposes the static correction method of converted wave based on PPS converted refracted wave,and processes the real three-component seismic data with better results of static correction of converted wave.

关键词： PPS converted refracted wave converted wave static correction

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Fluorescence dynamics of interactions between polyamide PyPyPyβDp and DNA

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Science China Chemistry 2006年第1期49卷 75-80页

作者： ZHANG Huijuan1,2. WANG Jin3. WU Yishi1, YUAN Gu3. AI Xicheng1, WANG Li4 & ZHANG Jianping1 1. {2. key laboratory for Structural Chemistry of Unstable and Stable Species,{3. of Chemistry, chinese academy of Sciences, beijing 100080, China 2. School of Life Science and Technology, beijing{3. of Technology, beijing 100081, China 3. College of Chemistry and Molecular Engineering, Peking University, beijing 100080, China 4. Center for Condensed Matter Physics,{3. of Physics, chinese academy of Sciences, beijing 100080, China Correspondence should be addressed to Ai Xicheng and Zhang Jianping 1. state key {2. for Structural Chemistry of Unstable and Stable Species Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China 2. School of Life Science and Technology Beijing Institute of Technology Beijing 100081 China 3. College of Chemistry and Molecular Engineering Peking University Beijing 100080 China 4. Center for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100080 China

The photophysical properties of the polyamide PyPyPyβDp (PPP) were investigated by means of steady-state absorption and fluorescence spectroscopies, as well as time-resolved fluo-rescence spectroscopy. It was found that the excited-state properties of PPP are very sensitive to solvents. In TKMC buffer PPP exhibited weak fluorescence with a decay time constant of 16 ps, while with the decrease of the solvent polarity PPP showed the blue-shifted peak position, increased inten-sity and lengthened life-time for its fluorescence behavior. In the presence of calf thymus DNA, it was observed that the fluorescence intensity was enhanced and the fluorescence lifetime increased from 16 to {2. ps for PPP, which verified that PPP bound into the minor groove of DNA duplex.

关键词： polyamide (PPP), calf thymus DNA (ctDNA), time-resolved fluorescence spectroscopy, recognition dynamics.

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