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生物物理学报 2009年第S1期25卷 217-218页

作者： Shusen Liu state key {2. of {3. and membrane biotechnology, Instutute of zoology, chinese academy of sciences, Beijing, 100101, china

Although some fusogenic proteins have been established to tether two opposite outer or inner mitochondrial membranes into contact, a prerequisite for whole mitochondrial fusion events,

关键词： Mitochondrial membrane fusion Protein-tethering Lipid-bilayer-mixing and fusion Interfacial △pH of PMF.Inter-play between membrane fusion and ATP synthesis.

Dynamics of morphological changes for mitochondrial fission and fusion

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Science china(Physics,Mechanics & Astronomy) 2010年第4期53卷 680-689页

作者： WANG ShiQi 1,2.4 , FU ChangLiang 1,2., ZHANG Yan 1,2., CHEN Quan 3.& LONG Mian 1,2.1 key {2. of Microgravity (National Microgravity {2.), Beijing 100190,{3. 2.Center for Biomechanics and Bioengineering, Institute of Mechanics, chinese academy of sciences, Beijing 100190,{3. 3.state key laboratory of biomembrane and membrane biotechnology, Institute of zoology, chinese academy of sciences, Beijing 100101,{3. 4 Institute of Vertebrate Paleontology and Paleoanthropology, chinese academy of sciences, Beijing 100044,{3. 1. key laboratory of Microgravity (National Microgravity laboratory) Beijing 100190 China2. Center for Biomechanics and Bioengineering Institute of Mechanics Chinese Academy of Sciences Beijing 100190 China4. Institute of Vertebrate Paleontology and Paleoanthropology Chinese Academy of Sciences Beijing 100044 China3. State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China

Mitochondria experience continuous fusion and fission in a living cell, but their dynamics remains poorly quantified. Here a theoretical model was developed, upon a simplified population balance equation (PBE), to predict the morphological changes induced by mitochondrial fission and fusion. Assuming that both fission and fusion events are statistically independent, the survival probability of mitochondria staying in the fission or fusion state was formulated as an exponentially-decayed function with time, which depended on the time-dependent distribution of the mitochondrial volume and the fission and fusion rates. Parametric analysis was done for two typical volume distributions. One was Gamma distribution and the other was Gaussian distribution, derived from the measurements of volume distribution for individual mitochondria in a living cell and purified mitochondria in vitro. The predictions indicated that the survival probability strongly depended on morphological changes of individual mitochondria and was inversely correlated to the fission and fusion rates. This work provided a new insight into quantifying the mitochondrial dynamics via monitoring the evolution of the mitochondrial volume.

关键词： mitochondria morphology fission fusion dynamics modeling

Plant protein kinase genes induced by drought, high salt and cold stresses

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chinese Science Bulletin 2000年第13期45卷 1153-1157页

作者： LIU Qiang , ZHANG Yong & Chen Shouyi1. Deparlmenl of Biological Science and biotechnology, Tsinghua University, Beijing 100084, china 2. Department of Biological Science and biotechnology, Slate {3. laboratory of biomembrane and membrane biotechnology, Tsinghua University, Beijing 100084, china 3. Institute of Genetics, chinese academy of sciences, Beijing 100101, china Department of Biological Science and biotechnology Tsinghua University Beijing China Department of Biological Science and biotechnology State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China Institute of Genetics Chinese Academy of Sciences Beijing China

Drought, high salt and cold are three different kinds of environment stresses that severely influence the growth, development and productivity of crops. They all decrease the water state of plant cells, and consequently result in the harm of plant from water deficit. Several genes encoding protein kinases and induced by drought, high salt and low temperature have been isolated from Arabidopsis. These protein kinases include receptor protein kinase (RPK), MAP kinases, ribosomal-protein kinases and transcription-regulation protein kinase. The expression features of these genes and the regulatory roles of these protein kinases in stress response and signal transduction are discussed.

关键词： protein kinase environment stress signal transduction gene expression.

Negative regulation of TGF-β signaling in development

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Cell Research 2004年第6期14卷 441-449页

作者： Ye Guang CHEN, An Ming MENG The state key {2. of {3. and membrane biotechnology, Department of Biological sciences and biotechnology, Tsinghua University, Beijing 100084, china Department of Biological sciences and biotechnology The State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing 100084 China

The TGF-β superfamily members have important roles in controlling patterning and tissue formation in both invertebrates and vertebrates. Two types of signal transducers, receptors and Smads, mediate the signaling to regulate expression of their target genes. Despite of the relatively simple signal transduction pathway, many modulators have been found to contribute to a tight regulation of this pathway in a variety of mechanisms. This article reviews the negative regulation of TGF-β signaling with focus on its roles in vertebrate development.

关键词： TGF-β BMP Nodal Activin antagonist development negative regulation.

GM-CSF-induced Ca_(2.) -activated non-voltage-dependent inwardly rectifying K_+ channel in murine peritoneal exudate macrophages

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chinese Science Bulletin 1997年第11期42卷 947-952页

作者： ZHANG Xiaofeng, FENG Meifu, WU Caihong and ZHOU Pei’aistate key {2. of {3. and membrane biotechnology, chinese academy of sciences, Beijing 100080, china 1 . In-stitute of zoology, chinese academy of sciences, Beijing 100080, china 2.College of Life sciences, Peking University, Beijing 100871, china state key {2. of {3. and membrane biotechnology Chinese Academy of Sciences Beijing 100080 China

GRANULOCYTE-MACROPHAGE colony-stimulating factor(GM-CSF),a member in the family ofglycoprotein cytokines,can not only act to stimulate proliferation of immature progenitors,butalso enhance differentiated functions of mature effector *** has two kinds of biologicaleffects on mature effector cells,*** effect and indirect *** latter is also

关键词： K+ channel patch clamp GM-CSF macrophage.

Electrophysiological research of synapse formation from beginning to maturity

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chinese Science Bulletin 1998年第1期43卷 55-59页

作者： XIE Zuoping and ZHENG Yufang state key {2. of {3. and membrane Biotechnique Department of Biological sciences and biotechnology, Tsinghua University, Beijing 100084, china state key {2. of {3. and membrane Biotechnique Tsinghua University Beijing China Department of Biological sciences and biotechnology Tsinghua University Beijing China

The development of synapses is an important question in neurobiology. We employ patchclamp technique to record MEPCs and EPCs of myoballs in Xenopus cell culture. The rate of EPC/MEPC is related with synapses maturation. Accordingly, we define the mature synapses as those whose rate of presynaptic membrane’s EPC to MEPC is more than 8. The process of maturation needs about 2.5 h after neurites contact with myoballs. We divide the development of synapses into three stages: young, developing and mature. The neurotransmitters stack gradually and increase by hundreds of times along with young synapses growing into maturity. So the process of synapses maturation should include presynaptic membrane specialization, postsynaptic membrane specialization and neurotransmitters stack.

关键词： definition of mature synapse α latrotoxin (α LTX) neurotransmitter stack.

An analysis on the evolvement processes of lake eutrophication and their characteristics of the typical lakes in the middle and lower reaches of Yangtze River

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chinese Science Bulletin 2006年第13期51卷 1603-1613页

作者： CHENG Xiaoying1,2.3.& LI Shijie1 1. Nanjing Institute of Geography & Limnology, chinese academy of sciences, Nanjing 2.0008,{2. 2. The key {3. of Industrial biotechnology, Ministry of Educa- tion, Southern Yangtze University, Wuxi 2.403.,{2. 3. School of biotechnology, Southern Yangtze University, Wuxi 2.403.,{2. Nanjing Institute of Geography & Limnology Chinese Academy of Sciences Nanjing China The key laboratory of Industrial {2. Ministry of Education Southern Yangtze University Wuxi China School of biotechnology Southern Yangtze University Wuxi China

According to the study on the aquatic environment of the typical lakes in the middle and lower reaches of Yangtze River, lake eutrophication is a process in which the lake ecosystem degenerates, resulting in the deterioration of the water quality due to the enrichment of the nutrients. Moreover, the succession of lake ecosystem and change of water quality both couple well with the evolvement of tro- phic state. Then the conceptual model is mentioned and ten lake states are classified. Based on these, the authors analyzed the eutrophication processes of the typical lakes in the middle and lower reaches of Yangtze River. The large lakes with taking in - send- ing out water typical of Poyang Lake evolve from state 1 before the 1980s into state 2.after the 1980s, the large and medium lakes typical of Taihu Lake evolve from state 1-2.in the 1960s into state 8 in the 1980s then into state 9 in the 1990s, the medium and small shallow lakes typical of Honghu Lake evolve from state 1 in the 1960s into state 2.after the 1980s and the small city (suburban) lakes typical of Donghu Lake evolve from state 2.in the 1960s into state 9 after the 1970s. Further, the 1980s is the key con- version period of the lakes of various types typical of Poyang Lake, Honghu Lake and Taihu Lake con- verting into mesotrophic state, the 1990s is that of Taihu Lake into eutrophic state and the 1970s is thatof the small city (suburban) lakes typical of Donghu Lake into eutrophic state. The series of nutrient evolvement of different typical lakes are similar, but the processes are not synchronous, which is related to the various human and natural driving mechanisms in lake basins.

关键词：长江湖泊富营养化概念模型转变时期驱动机制

Structure and regulatory function of plant transcription factors

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chinese Science Bulletin 2001年第4期46卷 271-278页

作者： LIU Qiang, ZHANG Guiyou & CHEN ShouyiDepartment of Biological Science and biotechnology, Tsinghua Univorsity, Beijing 100084, china Department of Biological Science and biotechnology, {3. laboratory of biomembrane and membrane biotechnology, Tsinghua University, Beijing 100084, china Institute of Genetics, chinese academy of sciences, Beijing 100101, china Department of Biological Science and biotechnology Tsinghua University Beijing China Department of Biological Science and biotechnology State Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China Institute of Genetics Chinese Academy of Sciences Beijing China

The expression of inducible genes in plants is regulated by specific transcription factors at the transcrip-tional level. A typical transcription factor usually contains a DNA-binding domain, a transcription regulation domain, a dimerization site and a nuclear localization domain. These functional domains define the characteristic, localization and regulatory role of a transcription factor. Transcription factors recognize and bind to specific cis-acting elements or interact with other proteins, and then activate or repress the transcription of target genes by their functional domains. In recent years, elucidation on the structure and function of transcription factors has become an important subject in plant molecular biology.

关键词： transcription factor DNA-binding domain cis-acting element.

Regulation of embryonic stem cell self-renewal and differentiation by TGF-β family signaling

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Science china(Life sciences) 2010年第4期53卷 497-503页

作者： FEI Teng & CHEN Ye-Guang The state key {2. of {3. and membrane biotechnology, School of Life sciences, Tsinghua University, Beijing 100084, china 1. The state key {2. of {3. and membrane biotechnology School of Life Sciences Tsinghua University Beijing 100084 China

Embryonic stem (ES) cells are characterized by their ability to indefinitely self-renew and potential to differentiate into all the cell lineages of the body. ES cells are considered to have potential applications in regenerative medicine. In particular, the emergence of an ES cell analogue-induced pluripotent stem (iPS) cells via somatic cell reprogramming by co-expressing a limited number of critical stemness-related transcriptional factors has solved the problem of obtaining patient-specific pluripotent cells, encouraging researchers to develop more specific and functional cell lineages from ES or iPS cells for broad therapeutic applications. ES cell fate choice is delicately controlled by a core transcriptional network, epigenetic modification profiles and complex signaling cascades both intrinsically and extrinsically. Of these signals, transforming growth factor β (TGF-β) family members, including TGF-β, bone morphogenetic protein (BMP), Activin and Nodal, have been reported to influence cell self-renewal and a broad spectrum of lineage differentiation in ES cells, in accordance with the key roles of TGF-β family signaling in early embryo development. In this review, the roles of TGF-β family signals in coordinating ES cell fate determination are summarized.

关键词： embryonic stem cell TGF-β BMP Activin Nodal self-renewal differentiation

Molecular cloning of growth hormone receptor (GHR) from common carp(Cyprinus carpio L.)and identification of its two forms of mRNA transcripts

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Progress in Natural Science:Materials International 2006年第11期16卷 1156-1163页

作者： SUN Xiaofeng~ 1,2., GUO Qionglin~1**, HU Wei~1, WANG Yaping~1 and ZHU Zuoyan~1(1. state key {2. of Freshwater Ecology and {3., Institute of Hydrobiology, chinese academy of sciences, Wuhan 43.072. china 2. Graduate School of chinese academy of sciences, Beijing 10003., china) [a]state key {2. of Freshwater Ecology and {3. Institute of Hydrobiology Chinese Academy of Sciences Wuhan China China [b]Graduate School of chinese academy of sciences Beijing China

The cDNA of growth hormone receptor (GHR) was cloned from the liver of 2.year common carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE). Its open reading frame (ORF) of 1806 nucleotides is translated into a putative peptide of 602.amino acids, including an extracellular ligand-binding domain of 2.4 amino acids (aa), a single transmembrane domain of 2. aa and an intracellular signal-transduction domain of 3.4 aa. Sequence analysis indicated that common carp GHR is highly homologous to goldfish (Carassius auratus) GHR at both gene and protein levels. Using a pair of gene-specific primers, a GHR fragment was amplified from the cDNA of 2.year common carp, a 2.4 bp product was identified in liver and a 3.1 bp product in other tissues. The sequencing of the products and the partial genomic DNA indicated that the difference in product size was the result of a 97 bp intron that alternatively spliced. In addition, the 3.1 bp fragment could be amplified from all the tissues of 4-month common carp including liver, demonstrating the occurrence of the alternative splicing of this intron during the development of common carp. Moreover, a semi-quantitative RT-PCR was performed to analyze the expression level of GHR in tissues of 2.year common carp and 4-month common carp. The result revealed that in the tissues of gill, thymus and brain, the expression level of GHR in 2.year common carp was significantly lower than that of 4-month common carp.

关键词： growth hormone receptor (GHR) molecular cloning transcript expression analysis common carp.