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Loss of Posterior Silk Gland Transcription Specificity of Fibroin Light Chain Promoter due to Absence of 41 bp Sequence Containing Possible Inhibitor Binding Sites

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Acta Biochimica et Biophysica Sinica 2005年第12期37卷 819-825页

作者： Ting-Qing GUO~(1,2) Jian-Yang WANG~(1,2) Sheng-Peng WANG~(1,2.3. Xiu-Yang GUO~(1,2) Ke-Wei HUANG3.3.Jun-Ting HUANG~3.Chang-De LU~11 institute of Biochemistry and Cell Biology,Shanghai institutes for Biological sciences.{3. Academy of sciences.Shanghai 20003..china 2 Graduate School of the {3. Academy of sciences.Shanghai 20003..china 3.serictdtu research institute.chinese Academy of agricultural sciences,zhenjiang 212018.china institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Graduate School of the chinese Academy of sciencesShanghai 20003. China Sericultural research institute Chinese Academy of Agricultural SciencesZhenjiang 212018 China

The gene encoding fibroin light chain protein (FibL) is specifically expressed in the posteriorsilk gland of silkworm and repressed in other *** binding sites of several transcription factorsrevolved in the silk gland transcription specificity of fibl promoter have been recognized,including SGFB,PSGF and *** we report the leak expression of the enhanced green fluorescent protein (EGFP)reporter gene in tissues other than the posterior silk gland in vivo when under the control of a shortened fiblpromoter with deletion of the 5′terminal 41 bp sequence,which is located at -650 nt to -610 nt upstream *** transcription starting *** of silk gland specificity of the promoters was performed by observationof green fluorescence in tissues of silkworm larvae following inter-haemocoelic injection of recombinantAutographa californica multiple nuclear polyhedrosis virus carrying the EGFP reporter gene controlled bydifferent lengths of fibl *** results indicated that availability of the binding sites of several knownfactors,including SGFB,PSGF and BMFA,is not sufficient for intact silk gland transcription specificity offibl promoter,and there are possible inhibitor binding sites in the 41 bp sequence (-650 nt to -610 nt)upstream of the transcription starting site which may be required to repress the activity of fibl promoter inother tissues.

关键词： promoter specificity fibroin light chain silkworm Bombyx mori recombinant AcMNPV

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Top 10 Influential Events in carbon neutrality and climate change response for 2023

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Frontiers in Energy 2024年第1期18卷 1-3页

作者： research institute of Carbon Neutrality of Shanghai Jiao Tong University research institute of Carbon Neutrality Shanghai Jiao Tong UniversityShanghai 200240China

In 2023. the global community intensified its efforts towards achieving carbon neutrality and addressing climate change, witnessing several impactful events. research institute of Carbon Neutrality, Shanghai Jiao Tong University compiled the Top 10 Events (in chronological order) in the field of global carbon neutrality and climate change response for 2023. to share with readers these significant moments that document the ongoing ambition and collective efforts of human society.

关键词： neutral collective intensified

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Structural Analysis of Fibroin Heavy Chain Signal Peptide of Silkworm Bombyx mori

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 507-513页

作者： Sheng-Peng WANG~(1,2)Ting-Qing GUO~1 Xiu-Yang GUO~1 Jun-Ting HUANG~2 Chang-De LU~(1*) 1 institute of Biochemistry and Cell Biology,Shanghai institutes for Biological sciences,{3. Academy of sciences,Shanghai 20003.,china 2 Sericultural research institute.chinese Academy of agricultural sciences,zhenjiang 212018.china institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Sericultural research institute Chinese Academy of Agricultural SciencesZhenjiang 212018 China

To study the minimal length required for the secretion of recombinant proteins and silkproteins in posterior silk gland,the signal peptide(SP)of the fibroin heavy chain(FibH)of silkwormBombyx mori was systematically shortened from the *** effect on the secretion of protein wasobserved using enhanced green fluorescent protein(EGFP)as a *** of EGFP fusion proteinswas examined under fluorescence *** SPs with lengths of 20,18,16 and 12 *** directthe secretion of the reporter,yet those with lengths of 11, 10, 9, 8 and 1 a.a. can not. When the FibH SP wasshortened to 12a.a., the secretion efficiency was decreased slightly and cleavage occurred within *** *** the FibH SP were used,the secretion of fusion protein was normal and the cleavage sitewas between the Gly-Ser linker and Met,the starting amino acid of EGFP. These findings are applicable forthe expression of foreign proteins in silkworm silk *** cleavage site of the SP is discussed andcompared with the predictive results of the SignalP 3.0 online prediction program.

关键词： signal peptide fibroin heavy chain silkworm Bombyx mori rAcMNPV

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中国政治经济学年度发展报告（2023.（下）

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政治经济学评论 2024年第3期 90+207-208页

作者： Center for Studies of Contemporary Political Economy,research institute for 21st-Century Marxism,Nankai University·University of chinese Academy of Social Science Center for Teaching and research on Capital,Renmin University of china National institute of Socialist Political Economy with chinese Characteristics,Renmin University of china 南开大学·中国社会科学院大学21世纪马克思主义研究院当代政治经济学研究中心中国人民大学《资本论》教学与研究中心中国人民大学全国中国特色社会主义政治经济学研究中心

编者按2023.，高质量发展扎实推进，新质生产力加快形成，我国经济总体回升向好，圆满实现发展主要预期目标。为针对性地破解经济恢复发展中的难题，聚焦推进中国式现代化进程中的重点、难点问题，中国政治经济学界开展系统深入研究，...

编者按2023.，高质量发展扎实推进，新质生产力加快形成，我国经济总体回升向好，圆满实现发展主要预期目标。为针对性地破解经济恢复发展中的难题，聚焦推进中国式现代化进程中的重点、难点问题，中国政治经济学界开展系统深入研究，取得系列代表性成果。为准确刻画学界研究进展和轨迹，推动中国经济学理论体系的构建和完善，本刊精选议题如期奉上《中国政治经济学年度发展报告（2023.》。自2010年起，本刊连续十四年发布《中国政治经济学年度发展报告》，“年度发展报告”专栏已成为本刊标志性特色栏目。2023.报告聚焦12个专题，分为（上）（下）两篇，其中专题1—6于报告（上）篇刊登，专题7—12由报告（下）篇刊登。2023.报告（下）选题如下：

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Refinement modeling and verification of secure operating systems for communication in digital twins

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Digital Communications and Networks 2024年第2期10卷 304-314页

作者： zhenjiang Qian Gaofei Sun Xiaoshuang Xing Gaurav Dhiman School of Computer Science and Engineering Changshu Institute of TechnologySuzhou215500China University Centre for research and Development Department of Computer Science and EngineeringChandigarh UniversityMohali140413India Department of Computer Science and Engineering Graphic Era Deemed to be UniversityDehradun248002India

In traditional digital twin communication system testing,we can apply test cases as completely as possible in order to ensure the correctness of the system implementation,and even then,there is no guarantee that the digital twin communication system implementation is completely *** verification is currently recognized as a method to ensure the correctness of software system for communication in digital twins because it uses rigorous mathematical methods to verify the correctness of systems for communication in digital twins and can effectively help system designers determine whether the system is designed and implemented *** this paper,we use the interactive theorem proving tool Isabelle/HOL to construct the formal model of the X86 architecture,and to model the related assembly *** verification result shows that the system states obtained after the operations of relevant assembly instructions is consistent with the expected states,indicating that the system meets the design expectations.

关键词： Theorem proving Isabelle/HOL Formal verification System modeling Correctness verification

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Enhanced Hoek-Brown(H-B)criterion for rocks exposed to chemical corrosion

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International Journal of Mining Science and Technology 2024年第5期34卷 609-630页

作者： Hao Li Leo Pel zhenjiang You David Smeulders Department of Mechanical Engineering Eindhoven University of TechnologyEindhoven 5600 MBthe Netherlands Department of Applied Physics Eindhoven University of TechnologyEindhoven 5600 MBthe Netherlands Center for Sustainable Energy and Resources School of EngineeringEdith Cowan UniversityJoondalupWA 6027Australia School of Chemical Engineering The University of QueenslandBrisbaneQLD 4072Australia Gas and Energy Transition research Centre The University of QueenslandBrisbaneQLD 4072Australia

Underground constructions often encounter water environments,where water–rock interaction can increase porosity,thereby weakening engineering ***,the failure criterion for chemically corroded rocks becomes essential in the stability analysis and design of such *** study enhances the applicability of the Hoek-Brown(H-B)criterion for engineering structures operating in chemically corrosive conditions by introducing a kinetic porosity-dependent instantaneous mi(KPIM).A multiscale experimental investigation,including nuclear magnetic resonance(NMR),X-ray diffraction(XRD),scanning electron microscopy(SEM),pH and ion chromatography analysis,and triaxial compression tests,is employed to quantify pore structural changes and their linkage with the strength responses of limestone under coupled chemical-mechanical(C-M)*** employing ion chromatography and NMR analysis,along with incorporating the principles of free-face dissolution theory accounting for both congruent and incongruent dissolution,a kinetic chemical corrosion model is *** model aims to calculate the kinetic porosity alterations within rocks exposed to varying H+concentrations and ***,utilizing the generalized mixture rule(GMR),the kinetic porositydependent mi is *** of the KPIM-enhanced H-B criterion using compression test data from 5 types of rocks demonstrated a high level of consistency between the criterion and the experimental results,with a coefficient of determination greater than 0.96,a mean absolute percentage error less than 4.84%,and a root-mean-square deviation less than 5.95 ***,the physical significance of the porosity-dependent instantaneous mi is clarified:it serves as an indicator of a rock’s capacity to leverage the confining pressure effect.

关键词： Hoek-Brown(H-B)criterion Instantaneous mi Kinetic porosity-dependency Chemical corrosion Compressive strength

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Potential land for plantation of Jatropha curcas as feedstocks for biodiesel in china

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Science china Earth sciences 2010年第1期53卷 120-127页

作者： WU WeiGuang 1,2,3.HUANG JiKun 1,3.& DENG XiangZheng 1,3.1 institute of Geographic sciences and Natural Resources research,{3. Academy of sciences,Beijing 100101,china 2 Department of Economics and Management,Zhejiang Forestry University,Linan 3.13.0,china 3.Center for {3. agricultural Policy,{3. Academy of sciences,Beijing 100101,china institute of Geographic sciences and Natural Resources research Chinese Academy of Sciences Beijing China Department of Economics and Management Zhejiang Forestry University Linan China Center for chinese agricultural Policy Chinese Academy of Sciences Beijing China

As a renewable energy,biofuel has attracted great attention in china and the rest of *** with the national food security,china recently has shifted its biofuel development priority from grain-based to non-grain-based biofuels,including forest-based biodiesel,since *** curcas is one of major biodiesel ***,there is rising debate on availability of land for expanding Jatropha curcas *** overall goal of this paper is to evaluate potential land for Jatropha curcas used as feedstock for biodiesel in *** on remote sensing data on land use,data on meteorological,soil and land slope,and suitable environment for Jatropha curcas plantation,this study uses Agro Ecological Zone method and considers social-economic constraints to evaluate potential suitable land for Jatropha curcas plantation in china's major Jatropha curcas production region,Southwest *** results show that while there are some potential lands to expand Jatropha curcas areas,amount of these lands will hardly meet the government's target for Jatropha curcas-based biodiesels development in the *** may need to reconsider its long-term targets on the development of Jatropha curcas-based biodiesels.

关键词： biodiesel Jatropha curcas potential land suitability evaluation Southwest china

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Relationships between tree-ring width index and NDVI of grassland in Delingha

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chinese Science Bulletin 2006年第9期51卷 1106-1114页

作者： HE Jicheng1,3.& SHAO Xuemei1,21. institute of Geographical sciences and Natural Resources research, {3. Academy of sciences, Beijing 100101, china 2. institute of Tibetan Plateau research, {3. Academy of sciences, Beijing 100085, china 3. Graduate School of {3. Academy of sciences, Beijing 10003., china institute of Geographical sciences and Natural Resources research Chinese Academy of Sciences Beijing China Graduate School of chinese Academy of sciences Beijing China institute of Tibetan Plateau research Chinese Academy of Sciences Beijing China

Using five well-replicated Qilian juniper (Sabina przewalskii Kom.) tree-ring width index se- ries, monthly normalized difference vegetation index (NDVI) of grassland, and climatic data from 1982 to 2001, the relationships between tree-ring width index, NDVI of grassland, and climatic data were analyzed firstly. Then, the relationship between tree-ring width index and NDVI of grassland was explored. The re- sults showed that: (1) Temperature and precipitation in June influenced tree-ring width index and NDVI of grassland deeply in Delingha. (2) There were sig- nificant relationships between five tree-ring width index series (DLH1-DLH5) and monthly NDVI of grassland from June to September, with the most significant relationship being between tree-ring width index series and NDVI of grassland in August. (3. The PC1 (the first principal component derived from DLH1-DLH5 series) exhibited good agreement with monthly NDVI of grassland in the grass growth sea- son (from June to September) and the averaged NDVI in the growth season, which was attributed to their common responses to water-supply limit in Delingha. This study may allow an increase in studying the past dynamics of grassland in Delingha in that the variation of grassland NDVI during the last millennium has been reconstructed from PC1.

关键词：圆柏祁连山 NDVI 草原年轮宽度指数

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Spectral analysis of quasi-stationary sea surface topography from GRACE mission

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Science china Earth sciences 2005年第11期48卷 2040-2048页

作者： ZHANG Zizhan1,2 & LU Yang1,3.1. institute of Geodesy and Geophysics, chinese Academy of sciences, Wuhan 43.077, {3. 2. Graduate School of the chinese Academy of sciences, Beijing 10003., {3. 3. Unite Center for Astro-geodymics research, chinese Academy of sciences, Shanghai 20003., {3. institute of Geodesy and Geophysics Chinese Academy of Sciences Wuhan China Graduate School of the chinese Academy of sciences Beijing China Unite Center for Astro-geodymics research Chinese Academy of Sciences Shanghai China

During the last two decades satellite altimetry has offered an abundance of meas-urements of the sea surface resulting in the improvement of global mean sea surface height (MSSH) and marine geoid determination. On the other hand, with the launching of new genera-tion gravity satellites, some high accuracy long-wavelength gravity models are available. These breakthroughs give us a great opportunity for new estimation of quasi-stationary sea surface topography (QSST). In this paper, the new gravity model GGM01C derived from GRACE mission is briefly presented, and a new global high precision and high-resolution QSST is determined based on the GGM01C model and the global MSSH. The spectral features of the QSST esti-mated by GGM01C and EGM96 gravity model to degree/order 200 are discussed by spectral analysis. As a result, the QSST is mainly composed of long waves, medium waves partially and short waves scarcely, its power spectral structures are different between the zonal direction and the meridional direction, there are great differences between the two models, which maybe ex-plain why the ocean currents derived from the two gravity models by Tapley show different pat-terns.

关键词： quasi-stationary sea surface topography, mean sea surface height, GGM01, fast Fourier transform, spectral analysis

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Debris Flow Hazard Assessment Based on Support Vector Machine

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Wuhan University Journal of Natural sciences 2006年第4期11卷 897-900页

作者： YUAN Lifeng 1, 2 , ZHANG Youshui 3. 1. institute of Mountain Hazards and Environment, chinese Academy of sciences, Chengdu 610041, Sichuan, {3. 2. Graduate University of chinese Academy of sciences, Beijing 100049, {3. 3. institute of Geographical sciences and Natural Resources research, chinese Academy of sciences, Beijing 100101, {3. institute of Mountain Hazards and Environment Chinese Academy of Sciences Chengdu Sichuan China Graduate University of chinese Academy of sciences Beijing China institute of Geographical sciences and Natural Resources research Chinese Academy of Sciences Beijing China

Seven factors, including the maximum volume of once flow , occurrence frequency of debris flow , watershed area , main channel length , watershed relative height difference , valley incision density and the length ratio of sediment supplement are chosen as evaluation factors of debris flow hazard degree. Using support vector machine （SVM） theory, we selected 259 basic data of 3. debris flow channels in Yunnan Province as learning samples in this study. We create a debris flow hazard assessment model based on SVM. The model was validated though instance applications and showed encouraging results.

关键词： debris flow hazard assessment support vector machine (SVM)

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