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Induction of Epstein-Barr Virus Lytic Replication by Recombinant Adenoviruses Expressing the Zebra Gene with EBV Specific Promoters

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Acta Biochimica et Biophysica Sinica 2005年第4期37卷 215-220页

作者： Lu CHEN Juan YIN Yi CHEN Jiang ZHONG Department of Microbiology and Microbial Engineering,school of life sciences,fudan university,shanghai 20043.,china Department of Microbiology and Microbial Engineering School of Life Sciences Fudan University Shanghai 200433 China

The latent Epstein-Barr virus (EBV) is found in the cells of many *** example,EBVis detectable in almost all cases,and in almost all tumor cells,of non-keratinizing nasopharyngeal *** the latent virus,which will result in its lytic replication and the death of tumor cells,is a potentialapproach for the treatment of EBV-associated *** this study,three recombinant adenoviruses wereconstructed to express the Zebra gene,an EBV gene responsible for switching from the latent state to ***-specific promoters were used in order to limit Zebra expression in EBV-positive cells,andreduce the potential side *** EBV promoters used were Cp,Zp and a dual promoter combining *** Zebra protein was detected in HEK293 cells as well as the EBV-positive D98-HR1cells infected with recombinant *** EBV lyric replication early antigen,EA-D,was also detected ininfected D98-HR1,implying the initiation of lytic *** the cell viability assay,Zebra-expressingadenoviruses had little effect on EBV-negative HeLa cells,while significantly reducing the cell viability andproliferation of D98-HR1 *** results indicate that EBV virus promoters can be used in adenovirusvectors to express the Zebra gene and induce EBV lytic replication in D98-HR1 cells.

关键词： Epstein-Barr virus recombinant adenovirus lytic replication cancer

The fundamental solution of the Keldysh typeoperator

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Science china Mathematics 2009年第9期52卷 1829-1843页

作者： CHEN ShuXing school of Mathematical sciences, fudan university, shanghai 20043., china school of Mathematical sciences Fudan University Shanghai China

In this paper we discuss the fundamental solution of the Keldysh type operator $ L_\alpha u \triangleq \frac{{\partial ^2 u}} {{\partial x^2 }} + y\frac{{\partial ^2 u}} {{\partial y^2 }} + \alpha \frac{{\partial u}} {{\partial y}} $ , which is a basic mixed type operator different from the Tricomi operator. The fundamental solution of the Keldysh type operator with $ \alpha > - \frac{1} {2} $ is obtained. It is shown that the fundamental solution for such an operator generally has stronger singularity than that for the Tricomi operator. Particularly, the fundamental solution of the Keldysh type operator with $ \alpha < \frac{1} {2} $ has to be defined by using the finite part of divergent integrals in the theory of distributions.

关键词： mixed type equation Keldysh operator fundamental solution finite part of divergent integral 35M10 35A08

RADIAL BASIS FUNCTION INTERPOLATION IN SOBOLEV SPACES AND ITS APPLICATIONS

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Journal of Computational Mathematics 2007年第2期25卷 201-210页

作者： Manping Zhang (school of Mathematical sciences,fudan university,shanghai 20043.,china Institute of Computational Mathematics,Chinese Academy of sciences,Beijing 100080,china school of Mathematical sciences fudan university Shanghai 200433

In this paper we study the method of interpolation by radial basis functions and give some error estimates in Sobolev space H^k（Ω）（k 〉 1）. With a special kind of radial basis function, we construct a basis in H^k（Ω） and derive a meshless method for solving elliptic partial differential equations. We also propose a method for computing the global data density.

关键词： Sobolev space Radial basis function Global data density Meshless method

Local Existence of Smooth Solutions to the FENE Dumbbell Model

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Chinese Annals of Mathematics,Series B 2012年第4期33卷 501-520页

作者： Ge YANG11school of Mathematical sciences, fudan university, shanghai 20043., china. school of Mathematical sciences Fudan University Shanghai China

The author proves the local existence of smooth solutions to the finite extensible nonlinear elasticity (FENE) dumbbell model of polymeric flows in some weighted spaces if the non-dimensional parameter b > 2.

关键词： Fokker-Planck equation FENE model Degenerate parabolic equations Regularity

Mass spectrometry analysis of melanoma related O-glycans in sera

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Science china Chemistry 2010年第4期53卷 807-811页

作者： BAI Chen1,4, WU Gang1 & ZHAO Liang2,3 1Department of life Science, fudan university, shanghai 20043., china 2Department of Chemistry, fudan university, shanghai 20043., china 3Institutes of Biomedical sciences, fudan university, shanghai 20043., china 4Department of Food Science, shanghai Business school, shanghai 200235, china Department of life Science Fudan University Shanghai China Department of Food Science Shanghai Business School Shanghai China Department of Chemistry Fudan University Shanghai China Institutes of Biomedical sciences Fudan University Shanghai China

Glycomics comparison was carried out by screening melanoma serum biomarkers between C57 mice with and without B16 implanted. O-glycans were released from 10 μL sera by β-elimination, purified by Graphitized Carbon Cartridge Solid Phase Extraction (GCC-SPE) and analyzed by MALDI-QIT-TOF-MS. MS raw data were acquired and exported by Launchpad software. MATLAB was then applied for further data analysis. 10 Glycans were considered to have stable changes after B16 implantation and 5 of them were under structural analysis via MS/MS.

关键词： mass spectrometry glycomics melanoma biomarkers

Semantic Search among Heterogeneous Biological Databases Based on Gene Ontology

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Acta Biochimica et Biophysica Sinica 2004年第5期36卷 365-370页

作者： Shun-Liang CAO, Lei QIN1, Wei-Zhong HE1, Yang ZHONG2, Yang-Yong ZHU, and Yi-Xue LI1,3* Department of Computing and Information Technology, fudan university, shanghai 20043., china shanghai Center for Bioinformation 1 Technology, shanghai 200235, china school of life sciences, fudan university, shanghai 20043., china Bioinformation Center of shanghai Institutes for Biological sciences, the Chinese Academy of sciences, shanghai 200031, china Department of Computing and Information Technology Fudan University Shanghai 200433 China shanghai Center for Bioinformation Technology shanghai 200235 China school of life sciences Fudan University Shanghai 200433 China Bioinformation Center of shanghai Institutes for Biological sciences the Chinese Academy of Sciences Shanghai 200031 China

Semantic search is a key issue in integration of heterogeneous biological databases. In this paper, we present a methodology for implementing semantic search in BioDW, an integrated biological data warehouse. Two tables are presented: the DB2GO table to correlate Gene Ontology (GO) annotated entries from BioDW data sources with GO, and the semantic similarity table to record similarity scores derived from any pair of GO terms. Based on the two tables, multifarious ways for semantic search are provided and the corresponding entries in heterogeneous biological databases in semantic terms can be expediently searched.

关键词： Gene Ontology semantic search BioDW

Hyperbolic positive mass theorem under modified energy condition

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Science china Mathematics 2008年第12期51卷 2189-2197页

作者： XIE NaQing Institute of Mathematics, school of Mathematical sciences, fudan university, shanghai 20043., china Institute of Mathematics School of Mathematical Sciences Fudan University Shanghai China

We provide two new positive mass theorems under respective modified energy conditions allowing T00 negative on some compact set for certain modified asymptotically hyperbolic manifolds. This work is analogous to Zhang... 详细信息

关键词： positive mass theorem modified energy condition modified asymptotically hyperbolic 83C40 53C27

Global Existence of Classical Solutions for Some Oldroyd-B Model via the Incompressible Limit

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Chinese Annals of Mathematics,Series B 2006年第5期27卷 565-580页

作者： Zhen LEI school of Mathematical sciences, fudan university, shanghai 20043., china school of Mathematics and Statistics, Northeast Normal university, Changchun 130024, china. school of Mathematical sciences Fudan University Shanghai 200433 China school of Mathematics and Statistics Northeast Normal University Changchun 130024 China

In this paper, we prove local and global existence of classical solutions for a system of equations concerning an incompressible viscoelastic fluid of Oldroyd-B type via the incompressible limit when the initial data ... 详细信息

关键词： Incompressible limit Global existence Oldroyd model

OsFY,a Homolog of AtFY,Encodes a Protein that Can Interact with OsFCA-γ in Rice(Oryza sativa L.)

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 492-499页

作者： Qi LU Zheng-Kai XU Ren-Tao SONG shanghai Key Laboratory of Bio-energy Crop,school of life sciences,shanghai university shanghai 200444,china shanghai Key Laboratory of Bio-energy Crop School of Life Sciences Shanghai UniversityShanghai 200444 China

FCA and FY are flowering time related genes involved in the autonomous flowering pathwayin *** interacts with FY to regulate the alternative processing of FCA *** FCA/FY interaction is also required for the regulation of FLC expression,a major floral repressor in ***,it is not clear if the regulation of this autonomous flowering pathway is also present in monocotplants,such as ***,alternative RNA processing of OsFCA was observed in rice,which stronglysuggested the existence of an autonomous flowering pathway in *** this work,we cloned the cDNA ofthe autonomous flowering pathway gene OsFY from *** predicted OsFY protein contained a conserved7 WD-repeat region and at least two Pro-Pro-Leu-Pro motifs compared to Arabidopsis *** protein-protein interaction between OsFY and OsFCA-γ,the key feature of their gene function,was also demon-strated using the yeast two-hybrid *** GenBank database search provided evidence of expressionfor other autonomous pathway gene homologs in *** results indicate that the autonomous floweringpathway is present in monocots,and the regulation through FY and FCA interaction is conserved betweenmonocots and dicots.

关键词： OsFY autonomous flowering pathway OsFCA protein interaction yeast two-hybrid