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Relationship of dose-Effect and Time-Effect to Hyperbaric Oxygen Therapy on Hypoxic-Ischemic Brain damage in the Neonatal Rat

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中国当代儿科杂志 2001年第4期 450-454页

作者： LIU Li-Xu, YANG Yu-Jiadepartment of Pediatrics, Xiangya hospital, central south university, changsha 410008, china

Objective To find the best pressure and time of hyperbaric oxygenation (HBO) therapy by comparing different pressures and times in a rat model of neonatal hypoxic-ischemic brain damage (HIBd) and to provide the mechanisms of HBO therapy for neonatal clinical application. Methods 7-day-old Sd rats were randomly divided into a control(CON) group, HIBd group and HBO group. The HBO group was divided into 7 sub-groups according to the different HBO pressure: 1 ATA(H 1.0), 1.25 ATA(H 1.25), 1.5 ATA(H 1.5), 1.75 ATA(H 1.75), 2 ATA(H 2.0), 2.25 ATA(H 2.25) and 2.5 ATA(H 2.5) group. After choosing the best pressure we divided the rats into two groups according to the different HBO time: 30 min group (H 30 min) and 60 min group (H 60 min). All the rats(14-day-old) were killed at 7 days after HI. Body weight increasing, left/right brain weight ratio, neuropathologic examination and superoxide dismutase(SOd) and malondialdehyde(MdA) content in brain tissue were measured in each group. Results ①The weight increasing rate [( 72.91± 21.27)%] and the left/right brain weight ratio ( 0.6444± 0.1599) decreased obviously. At the same time the content of SOd [( 203.32± 57.18) NU/ml] in brain tissue decreased and that of MdA [( 4.197± 0.683) nmol/ml] increased in HIBd group. All above data showed a more significant difference in HIBd group compared to the control group [weight increasing rate=( 104.97± 17.34)%,left/right brain weight ratio= 0.9857± 0.0396,SOd content=( 261.53± 41.38) NU/ml,MdA content=( 3.393± 0.574) nmol/ml](Pd left/right brain weight ratio gradually increased, the content of SOd in brain tissue increased and that of MdA decreased, and to the best in H 2.0 group [left/right brain weight ratio= 0.8583± 0.1544,SOd content=( 259.20± 45.22) NU/ml,MdA content=( 3.462± 0.498) nmol/ml, compared to HIBd group, P< 0.01 or < 0.05]. ③The weight increasing rate, left/right brain weight ratio, the content o

关键词： Hypoxic-ischemic brain damage Hyperbaric oxygenation Therapy Neonatal rat

Inhibitory Effect Of Ad-El A On Human Laryngocarcinoma Cell Line Hep-2

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Inhibitory Effect Of Ad-El A On Human Laryngocarcinoma Cell ...

中国病理生理学会第十三届肿瘤、第十四届免疫专业委员会学术会议

作者： Yue-xiang QIN 1,Zhi-jie XU2,Xing-wei WANG 1 * 1 department of Otolaryngology,Xiangya hospital,central south university, changsha410078,china 2 Cancer Research institute,central south university,changsha 41 0078,china

Background and Objective:Adenovirus vector has been widely used to cancer gene ***,one of growth inhibiting factors,is a potent cancer suppressor gene that could lead to apoptosis in many cancer *** study was to investigate the inhibitory effect of adenovirus-mediated E IA(Ad-E IA) on human laryngocarcinoma cell line Hep-2 in ***:After transfection purified E 1A into Hep-2 cells,the expression of EIA was confirmed by ***,we used fluorescent microscopy to observe the cytotoxicity of Ad-ElA to Hep-2 *** influence of

关键词： Ad-El A Hep-2 proliferation apoptosis

Proteomics Profiling of Pituitary,Adrenal Gland,and Splenic Lymphocytes in Rats with Middle Cerebral Artery Occlusion

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Proteomics Profiling of Pituitary,Adrenal Gland,and Splenic ...

第六届全国中西医结合基础理论研究学术研讨会暨第二届湖南省中西医结合学会肝病专业学术年会

作者： Xingui XIONG,1 Qinghua LIANG,1,+ Jiang CHEN,2 Rong FAN,1 and Tianli CHENG 1 1.institute of Integrated Traditional Chinese Medicine and Western Medicine,Xiangya hospital,central south university,changsha,Hunan 410008,china 2.Center of Telemedicine,Xiangya hospital,central south university,changsha,Hunan 410008,china

Ischemic strokes are often accompanied by serious brain injury and poor prognosis,but the molecular mechanisms of primary and secondary injury have not been fully *** aim of the present study was to investigate the protein profile in the rat pituitary,adrenal gland,and splenic lymphocyte using proteomics techniques,and to elucidate potential changes in the immune

关键词： cerebral ischemia neuroendocrine-immune system proteomics mass spectrometry

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虎纹捕鸟蛛毒素Ⅴ的二硫键定位分析

生物化学与生物物理进展 2004年第6期31卷 550-555页

作者：张鹏飞肖顺勇梁宋平中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室长沙410008 湖南师范大学生命科学学院

虎纹捕鸟蛛毒素Ⅴ是从虎纹捕鸟蛛毒液中分离得到的一种昆虫毒素 .它含有 35个氨基酸残基 ,其中 6个半胱氨酸形成三对二硫键 .首先采用多酶将天然的肽链裂解后 ,通过MALdI TOF质谱分析酶解肽段 ,推断出 1对二硫键位于Cys9 Cys2 1,然后利... 详细信息

虎纹捕鸟蛛毒素Ⅴ是从虎纹捕鸟蛛毒液中分离得到的一种昆虫毒素 .它含有 35个氨基酸残基 ,其中 6个半胱氨酸形成三对二硫键 .首先采用多酶将天然的肽链裂解后 ,通过MALdI TOF质谱分析酶解肽段 ,推断出 1对二硫键位于Cys9 Cys2 1,然后利用改进的部分还原分步测序法 ,确定虎纹捕鸟蛛毒素Ⅴ的另外 2对二硫键的配对方式为Cys2 Cys16和Cys15 Cys2 8.因此 ,虎纹捕鸟蛛毒素Ⅴ的 3对二硫键分别以Cys2 Cys16 ,Cys9 Cys2 1,Cys15 Cys2 8(即 1 4、 2 5和 3 6 )的方式配对 .

关键词：虎纹捕鸟蛛毒素V 二硫键定位三羧甲基磷酸(TCEP)

Influences of Ti substitution on the structure and electrochemical properties of Mg_(1-x)Ti_xNi(x=0,0.1, 0.2, and 0.3) hydrogen storage alloys

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Rare Metals 2009年第5期28卷 504-510页

作者： HUANG Hongxia HUANG Kelong LIU Suqin CHEN dongyang College of Chemistry and Chemical Engineering Central South University Changsha 410083 China department of Materials and Chemical Engineering Guilin University of Technology Guilin 541004 China

MgNi-based hydrogen storage alloys Mg1–xTixNi （x = 0, 0.1, 0.2, and 0.3） were prepared by means of mechanical alloying. Mg in the alloy was partially substituted by Ti to improve the cycle stability of the alloys. The effects of the substitution of Ti for Mg on the microstructure and electrochemical performances of the alloys were investigated in detail. The results indicate that the substitution of Ti for Mg obviously decreases the discharge capacity, but it significantly improves their cycle stabilities. The microstructure of the alloys analyzed by X-ray diffraction （XRd） and scanning electron microscopy （SEM） shows that the alloys have a dominatingly amorphous structure. The substitution of Ti for Mg helps to improve the anti-oxidation/corrosion ability of the MgNi alloy but demolishes the electrochemical kinetics of hydrogenation/dehydrogenation. The Mg0.9Ti0.1Ni alloy electrode milled for 80 h exhibits the best integrative capability, which has the maximal discharge capacity of 331.66 mAh/g and the C30/Cmax of 63.65%.

关键词： hydrogen storage alloys Ti substitution structural characteristic electrochemical properties

Electronic Structure and Properties of Cu Metal~*

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Science china Mathematics 1993年第4期36卷 487-494页

作者： XIE You-Qing ZHANG Xiao-dong ZHAO Li-Ying and MA Xiu-Lin (department of Materials Science and Engineering, central south university of Technology, changsha 410083, PRC) 张晓东赵礼颖马秀林

The main purpose of the present work is to introduce a simple and effective method in order to analyse the structure and properties of both pure metals and alloys. The main points of this method are to use one-atom state instead of one-electron state and a potential function with many-atom interactions instead of Schrodinger equation. The atomic state of pure metals can be constructed by some basic atomic states which obey the Pauli exclusion principle. The electronic structure of Cu metal which is [Ar](3d_n)^(4.89)(3d_c)^(4.77)(4s_f)^(1.34) has been determined by self-conslstency method. Theoretical values of many properties of Cu metal, such as lattice constant, cohesive energy, bulk modulus and temperature dependence of thermal expansion coefficient calculated according to this electronic structure are in good agreement with experiments.

关键词： Cu electronic structrue valence bond theory potential curve elasticity thermal expansion coefficient

TIM and PRdX6,Two Novel Serum Markers for Human Lung Squamous Carcinoma

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TIM and PRDX6,Two Novel Serum Markers for Human Lung Squamou...

2008年全国生物化学与分子生物学学术大会

作者： Cui Li~1 Xiu-zhi Zhang~1 Zhi-qiang Xiao~1 Mao-Yu Li~1 Zhu-chu Chen~(1,2) 1 Key Laboratory of Cancer Proteomics of Chinese Ministry of health,Xiangya hospital,central south university,changsha china 2 Cancer Research institute,Xiangya School of Medicine,central south university,changsha china

There is currently substantial interest in the identification of human tumor antigens for diagnosis and immu- notherapy of *** our previous study,we have utilized proteomic approach for the identification of tumor proteins that elicit a humoral response in patients with lung squamous carcinoma (LSC).As a result,autoanti- bodies against triosephosphate isomerase (TIM) and peroxiredoxin 6 (PRdX6) were detected in sera from over

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肺炎衣原体蛋白激酶Cpn0148的克隆表达和定位

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微生物学通报 2009年第3期36卷 412-415页

作者：陈凡程文中南大学湘雅医院心血管内科湖南长沙410008 中南大学基础医学院免疫学系湖南长沙410078

目前认为肺炎衣原体(Chlamydia pneumonia,Cpn)除导致呼吸道疾病外,也是与冠心病相关的重要病原体。作为一种细胞内寄生的病原菌,Cpn激活宿主细胞信号通路,维护其在细胞内生长代谢,并导致疾病。肺炎衣原体基因Cpn0148可编码真核细胞样的... 详细信息

目前认为肺炎衣原体(Chlamydia pneumonia,Cpn)除导致呼吸道疾病外,也是与冠心病相关的重要病原体。作为一种细胞内寄生的病原菌,Cpn激活宿主细胞信号通路,维护其在细胞内生长代谢,并导致疾病。肺炎衣原体基因Cpn0148可编码真核细胞样的丝/苏氨酸蛋白激酶,利用PCR技术扩增全长Cpn0148ORF,将其定向插入pGEX-6p原核表达载体,在大肠杆菌XL-1blue中表达,测序显示Cpn0148ORF全长1860bp,编码619个氨基酸,分子量大约70kd,将Cpn0148-GST融合蛋白免疫BALB/c小鼠获得抗Cpn0148多克隆抗体,间接免疫荧光法检测显示Cpn0148表达在菌体上。Cpn0148蛋白质的获得为进一步研究Cpn与宿主细胞的相互作用奠定了基础。

关键词：肺炎衣原体 Cpn0148 丝/苏氨酸蛋白激酶

高效液相色谱法快速测定血清中的芳香族氨基酸

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色谱 2003年第2期21卷 154-157页

作者：文江平唐爱国中南大学湘雅二医院临床检验教研室湖南长沙410011

采用高效液相色谱紫外检测法分离测定了血清中的芳香族氨基酸。采用的色谱柱为WatersNova PakC18柱(4μm,150mm×3 9mmi d ),流动相为乙腈水(体积比为6∶94,pH3 4)溶液,流速为1 0mL/min,检测波长为215nm。血清标本经5%(体积分数)高氯... 详细信息

采用高效液相色谱紫外检测法分离测定了血清中的芳香族氨基酸。采用的色谱柱为WatersNova PakC18柱(4μm,150mm×3 9mmi d ),流动相为乙腈水(体积比为6∶94,pH3 4)溶液,流速为1 0mL/min,检测波长为215nm。血清标本经5%(体积分数)高氯酸溶液去除蛋白质后取上清液直接进样,10min内完成测定。探讨了流动相的pH及其有机相的比例、蛋白质沉淀剂以及检测波长等因素对分离度和灵敏度的影响。考察了其他10余种氨基酸、多巴胺类等物质对目标组分检测的干扰。该方法的日内、日间测定的相对标准偏差均小于5%,芳香族氨基酸测定的线性范围、检测限、回收率等均满足临床检验的要求。结果表明:该方法简便、快速、准确、可靠,适用于临床和科研工作。

关键词：测定血清高效液相色谱法芳香族氨基酸苯丙氨酸酪氨酸色氨酸临床诊断