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Energy Transfer among Chlorophylls in Trimeric Light-harvesting Complex Ⅱ of Bryopsis corticulans

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Acta Biochimica et Biophysica Sinica 2006年第5期38卷 310-317页

作者： Su-Juan ZHANG~(1,2*)Shui-Cai WANG~2 Jun-Fang HE~2 Hui CHEN~3 1 institute of Photonics and Photo-Technology,Northwest University,Xi'an 710069,china 2 State Key Laboratory of Transient Optics and Photonics,Xi 'an institute of Optics and Precision Mechanics,chinese academy of sciences,Xi'an 710068.china 3.laboratoty of photosynthesis basic research,institute of botany,chinese academy of sciences,beijing 100093.china institute of Photonics and Photo-Technology Northwest UniversityXi'an 710069 China State Key Laboratory of Transient Optics and Photonics Xi'an Institute of Optics and Precision Mechanics Chinese Academy of SciencesXi'an 710068 China Laboratory of photosynthesis basic research Institute of Botany Chinese Academy of SciencesBeijing 100093 China

A study on energy transfer among chlorophylls（Chls）in the trimeric unit of the major light-harvesting complex Ⅱ（LHC Ⅱ）from Bryopsis corriculan,was carried out using time-correlated singlephoton *** the chlorophyll Q region of LHC Ⅱ,six molecules characterized as Chlb628,Chlb646,Chlb652654,657,Chla664666,Chla674677.680and Chla682683were discriminated according to their absorption spectrumand fluorescence emission ***,excited by pulsed light of 628 nm,fluorescence kinetics spectrain the chlorophyll Q region were *** accordance with the principles of fluorescence kinetics,thesekinetics data were analyzed with a multi-exponential *** constants on energy transfer were *** overwhelming percentage of energy transfer among chlorophylls undergoes a process longer than 97picoseconds（ps）,which shows that,before transferring energy to another Chl,the excited Chl might convertenergy to vibrations of a lower state with different multiplicity（intersystem crossing）.Energy transfer at thelevel of approximately 10 ps was also obtained,which was interpreted as the excited Chls may go throughinternal conversion before transferring energy to another *** with a higher standard deviation,timeconstants at the femtosecond level can not be entirely excluded,which can be attributed to the ultrafastprocess of direct energy *** to the arrangement and direction of the dipole moment of Chls inLHC Ⅱ,the probability of these processes is *** fluorescence lifetimes of Chlb652654,657,Chla664666,Chla674677.680and Chla682683were determined to be 1.44ns,1.43 ns,636 ps and 713 ps,*** of energy dissipation in the pathway of fluorescence emission were no more than 40% in thetrimeric unit of LHC Ⅱ.These results are important for a better understanding of the relationship between thestructure and function of LHC Ⅱ.

关键词： energy transfer LHC Ⅱ transient spectrum fluorescence kinetics

Present status and development on biological nitrogen fixation research in china

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chinese Science Bulletin 2003年第10期48卷 954-960页

作者： SHEN Shihua & JING Yuxiang Key Laboratory of photosynthesis and Plant-Environment MolecularPhysiology, institute of botany, chinese academy of sciences, beijing 100093, china Key Laboratory of photosynthesis and Plant-Environment Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China

This presentation introduces the advances inbiological nitrogen fixation research abroad, in particular,describes the great progress and achievements on itsresearch in china as follows: collection of rhizobial resources and establishment of the largest database of Rhizobium inchina, correction and development of Rhizobium taxonomy in international; discovery of a couple of nif genes,identification and unification of linkage among the nif gene operons of Klebsiella pneumoniae, finding of regulative mechanism of positive regulation nif gene and its sensitivity to oxygen, temperature; finding of the activity of nodulation gene nodD3 product in Sinorhizobium meliloti which is notcontrolled by flavonoid produced from its host alfalfa;finding of the association between expression of genes coding the products for carbon utilization and nitrogen metabolism and their regulations; chemical synthesis of nodulationfactor of Sinorhizobium meliloti; constructions of engineered nitrogen fixers and utilization in practice based on theresearch of gene expression and regulation; chemicalsimulation of the structure and function of nitrogenase and bringing forward the model of nitrogenase active center for the first time in international and synthesis of modelcompounds which were paid attention by colleagues abroad. Finally, the development of nitrogen fixation research inchina in future has been put forward, suggesting that the nifgene regulation and its role in providing crops with nitrogen element, signal transduction and molecular interactions between Rhizobium and legume, coupling between carbonand nitrogen metabolisms, nitrogen fixation andphotosynthesis, and functional genomics of nitrogen-fixing nodule symbiosis, etc., would be actively worked on.

关键词：现状发展生物固氮技术中国固氮酶基因表达微生物植物相互作用化学模型

Thermal stability of oxygen evolution in photosystem Ⅱ core complex in the presence of digalactosyl diacylglycerol

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chinese Science Bulletin 2002年第24期47卷 2089-2092页

作者： YANG Zhenle, LI Liangbi & KUANG Tingyunphotosynthesis research Center, institute of botany, chinese academy of sciences, beijing 100093, china photosynthesis research Center Institute of Botany Chinese Academy of Sciences Beijing China

The influence of digalactosyldiacylglycerol (DGDG), one of the photosynthetic membrane lipids, on heat inactivation of the process of oxygen evolution has been studied in vitro in photosystem Ⅱ(PS Ⅱ) core complex. It was found that the temperature of semi-inactivation of oxygen evolution in the complex increased from 40.0 to about 43.0℃ in the presence of DGDG with 5-min heat treatment in the dark. Furthermore, when PS Ⅱ core complex was incubated for 5 min at 45.0℃, the oxygen evolution in the complex was completely lost, whilst the DGDG-complexed PS Ⅱ core complex still retained a 16% of activity (100% for 25.0℃). In addition, a 1-h incubation at 38.0℃ inactivated absolutely the oxygen evolution for the PS Ⅱ core complex. By contrast, there remained about 20% of activity (zero time for 100%) for the complex in the presence of DGDG under the same condition. These results indicate a new role of DGDG in the protection of PS Ⅱ core complex against the deleterious effects of temperature. It was

关键词： digalactosyl diacylglycerol photosystem Ⅱ core complex extrinsic protein of 33 kD thermal stability oxygen evolution.

The excitation energy transfer between β-Car and Chla molecules in PS Ⅱ core antenna complexes CP43 and CP47

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chinese Science Bulletin 2000年第17期45卷 1579-1583页

作者： SHAN Jixiu, WANG Jushuo, LI Liangbi,ZHAO Nanming & KUANG Tingyun1. Laboratory of photosynthesis basic research, institute of botany, chinese academy of sciences, beijing 100093, china 2. Department of Biological sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, beijing 100084, chinaCorrespondence should be addressed to Kuang Tingyun Laboratory of photosynthesis basic research Institute of Botany Chinese Academy of Sciences Beijing China Department of Biological sciences and Biotechnology State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China

The excitation energy transfer efficiency between p-Car and Chla molecules in purified CP43 and CP47 was calculated by comparing absorption and fluorescence excitation after normalization at 550 nm, CP43 had an energy transfer efficiency of 29.1% while the CP47 had an energy transfer efficiency of 62.8%, proving that excitation energy was transferred between p-Car and Chla molecules in CP43 and CP47 at normal conditions. The excitation energy transfer between p-Car and Chla molecules in CP43 and CP47 may occur through the "Dexter" mechanism and the distance between these two kinds of pigments should be less than 1 nm. In addition, the results were also used to discuss the conformational relationship between p-Car and Chla molecules in CP43 and CP47.

关键词： CP43 CP47 β-Car Chla excitation energy transfer.

Depletion of phosphatidyl-glycerol head-group induces changes in oxygen evolution and protein secondary structures of photosystem Ⅱ

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chinese Science Bulletin 2002年第10期47卷 824-829页

作者： YANG Zhenle, WANG Zeneng, JIANG Guizhen, XU Yinong, LI Liangbi & KUANG Tingyunphotosynthesis research Center, institute of botany, chinese academy of sciences, beijing 100093, china photosynthesis research Center Institute of Botany Chinese Academy of Sciences Beijing China

The techniques of oxygen electrode polarogra-phy and Fourier transform infrared (FT-IR) spectroscopy were employed to explore the roles of polar head-group of phosphatidylglycerol (PG) molecules in the functional and structural aspects of photosystem Ⅱ (PS Ⅱ) through enzymatic approach. It was shown that the depletion of PG by treatment of phospholipase C (PLC) on PS Ⅱ particles caused the inhibition of oxygen evolving activity in PS Ⅱ. This effect also gave rise to changes in the protein secondary structures of PS Ⅱ, that is, an increase in a-helical conformation which is compensated by the loss of p-strand structures. It revealed that the head-group of PG molecules plays an important structural role in the maintenance of normal structure of PS Ⅱ proteins, which is required to maintain the appropriate physiological activity of the PS Ⅱ complex such as the oxygen evolving activity. It is suggested that there most probably exist hydrogen-bonding interactions between PG molecules and PS Ⅱ proteins.

关键词： photosystem II polar head-group of phosphatidylglyc-erol phospholipase C oxygen evolution protein secondary struc-ture.

The structural model of P_(680)

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chinese Science Bulletin 1999年第9期44卷 862-863页

作者： ZHANG Chunxi , ZHANG Xiaodong , LI Liangbi , ZHANG Qiyuan , ZHANG Xingkang and KUANG Tingyunphotosynthesis research Center and Laboratory of photosynthesis basic research , institute of botany , chinese academy of sciences , beijing 100093, china State Key Laboratory for Structural Chemistry of Unstable and Stable Species , institute of Chemistry, chinese academy of sciences , beijing 100080, china photosynthesis research Center and Laboratory of photosynthesis Hasic research Institute of Botany Chinese Academy of Sciences Beijing China State Key Laboratory for Structural Chemistry of Unstable and Stuble Species. institute of Chemistry Chinese Academy of Sciences Beijing China

INVESTIGATION on photosystem Ⅱ （PS Ⅱ ） of high plants is one of the most important aspects in photosynthesis research. P680 is the primary electron donor in reaction center of PS Ⅱ , which is a crucial component for sun-light conversion and water split in PS Ⅱ. So study on its structure and function is very important. So far, a few structural models of P（680） have been proposed. Typical models are monomolecular model and bimolecular model. There are two types of bimolecular model: bimolecular parallel model and bimolecular angular model. (Note: There is a kind of multiple model. Because it can be obtained from the organization of two bimolecular models, this model is considered as the same sort of bi-

关键词： The structural model of P

Increase in electron transfer activity in photosystemⅡof spinach thylakoids caused by conversion of phosphatidyl-glycerol to phosphatidic acid molecules

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chinese Science Bulletin 2003年第15期48卷 1581-1585页

作者： WU Feng, YANG Zhenle, LI Liangbi & KUANG Tingyun Key Laboratory of photosynthesis and Environmental Molecular Physiology & photosynthesis research Center, institute of botany, chinese academy of sciences, beijing 100093, china Correspondence should be addressed to Yang Zhenle (e-mail: yangzhl @***) Key Lab. of Photosyn. and Environmental Molec. Physiology and photosynthesis research Center Institute of Botany Chinese Academy of Sciences Beijing 100093 China

The techniques of oxygen electrode polarography, sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE) and thin layer chromatography (TLC) were employed to investigate the effect of phospholipase D treatment on physiological function of spinach thylakoids. It was shown that the phospholipase D treatment on thylakoid resulted in the degradation of phosphatidylglycerol (PG) and occurrence of phosphatidic acid (PA). The changes of PG to PA molecules caused an increase in oxygen evolution in photosystem Ⅱ (PSⅡ), which was accompanied by an uncoupling effect on thylakoid membrane. It was revealed that the head-groups of PG molecules play an important role in the maintenance of the appropriate physiological activity of thylakoid membrane.

关键词：电子转移活性类囊体光合作用菠菜磷脂酰甘油

The characteristics of CO_2 assimilation of photosynthesis and chlorophyll fluorescence in transgenic PEPC rice

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chinese Science Bulletin 2001年第13期46卷 1080-1084页

作者： JIAO Demao, LI Xia, HUANG Xueqing, CHI Wei, KUANG Tingyun & Ku SB Maurice1. institute of Agrobiological Genetics and Physiology, Jiangsu academy of Agricultural sciences, Nangjing 210014, china 2. institute of botany, the chinese academy of sciences, beijing 100093, china 3. botany Department, Washington State University, Pullman, WA 99164-4236, USA institute of Agrobiological Genetics and Physiology Jiangsu Academy of Agricultural Sciences Nangjing China institute of botany The Chinese Academy of Sciences Beijing China botany Department Washington State University Pullman USA

With PEPC, PPDK, NADP-ME and PEPC+ PPDK transgenic and untransformed rice (Orysa sativa L.), the activities of related C4 photosynthesis enzymes, the chlorophyll fluorescence parameters, CO2 exchange and other physiological indexes were compared, in which the physiological characteristics of PEPC transgenic rice were mainly studied. The results were as follows: (i) The activities of PEPC in PEPC transgenic rice were 20-fold higher than those in untransformed rice; the light-saturation photosynthetic rates and the carboxylation efficiency of PEPC transgenic rice were increased by 55% and 50% more than those of untransformed rice, respectively, while the CO2 compensation point decreased by 27%. (ii) The PS II photochemical efficiency (Fv/Fm) and photochemical quenching (qp) of transgenic PEPC rice decreased less in comparison with those of untransformed rice after the treatment with high light intensity (3 h) or methyl viologen (MV), a photooxidative reagent, which demonstrated that the tolerance of PEPC

关键词： transgenic rice photosynthetic characteristics photoin-hibition of photosynthesis chlorophyll fluorescence phosphoenopy-ruvate carboxylase (PEPC).

Variations of near-surface atmospheric CO_(2)and H_(2)O concentrations during summer on Muztagata

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Science china Earth sciences 2006年第1期49卷 18-26页

作者： XU Baiqing1,2, YAO Tandong1,2, LU Anxin2, GUO Xuejun, LIU Xianqin2 & LI Zhen2 1. Laboratory of Environment and Process on Tibetan Plateau, institute of Tibetan Plateau research, chinese academy of sciences, beijing 100085, china 2. Key Laboratory of Cryosphere and Environment, Jointly Established by Cold and Arid Regions Environmental and Engi-neering research institute and institute of Tibetan Plateau research, chinese academy of sciences and chinese academy of Meteorological sciences, china Meteorological Administration, Lanzhou 730000, china Laboratory of Environment and Process on Tibetan Plateau Institute of Tibetan Plateau Research Chinese Academy of Sciences Beijing China Key Laboratory of Cryosphere and Environment Jointly Established by Cold and Arid Regions Environmental and Engineering Research Institute and Institute of Tibetan Plateau Research Chinese Academy of Sciences and Chinese Academy of Meteorological Sciences China Meteorological Administration Lanzhou China

Expeditions during the summers of 2002 and 2003 implemented continuous monitoring of near-surface (2 m height) atmospheric CO2 and H2O concentrations at the 4500 m elevation on Muztagata. The resultant data sets reveal a slight decrease of CO2 concentrations (of about 5 μmol·mol?1) and changes in the diurnal variations from the end of June to the middle August. The daily maximum CO2 concentrations occur between 02:30―05:30 AM (local time) and the minimum levels occur between 12:00―15:30 PM. The atmospheric CO2 concentrations in the summer of 2002 were around 5 μmol·mol?1 lower than those during the same period of 2003, whereas the diurnal amplitude was higher. In contrast, we found that the daily mean atmospheric H2O content in 2003 was much lower than that in 2002 and there exists a striking negative correlation between CO2 and H2O con-centrations. We therefore suggest that the near-surface atmospheric CO2 concentration is affected not only by photosynthesis and respiration, but also by the air H2O content in the glaciated region around Muztagata.

关键词： atmospheric CO2 and H2O concentration, continuous monitoring, glaciation region, Muztagata.