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粪产碱杆菌青霉素G酰化酶在大肠杆菌中组成型表达及分离纯化

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生物工程学报 2004年第5期20卷 736-740页

作者：杨志建蔡谨孙健袁中一中国科学院上海生命科学院生物化学与细胞生物学研究所上海200031 浙江大学化学工程与生物工程系杭州310027 中国科学院大连化学物理研究所大连116023

将粪产碱杆菌青霉素G酰化酶基因构建重组表达质粒pKKFPGA ,pKKFPGA再转化宿主菌DH5α,所得重组菌不需诱导便能高效表达青霉素G酰化酶 ,表达量达 2.5 90u L ,比野生型粪产碱杆菌表达量高 4 3.倍 ,其菌体比活力达 3. 0 (u L) A6 0 0 。... 详细信息

将粪产碱杆菌青霉素G酰化酶基因构建重组表达质粒pKKFPGA ,pKKFPGA再转化宿主菌DH5α,所得重组菌不需诱导便能高效表达青霉素G酰化酶 ,表达量达 2.5 90u L ,比野生型粪产碱杆菌表达量高 4 3.倍 ,其菌体比活力达 3. 0 (u L) A6 0 0 。菌体破碎后的上清液经DEAE_SepharoseCL 6B离子交换层析和Butyl_SepharoseCL 4B疏水层析 ,即可得纯度提高 2.0倍、比活为 6 8 6u mg的青霉素G酰化酶 ,两步纯化的总收率达 91%。Western印迹分析表明 5 %的原前体青霉素酰化酶在胞内形成了包涵体。

关键词：青霉素G酰化酶粪产碱杆菌表达纯化包涵体大肠杆菌

Pt／γ-Al2.3.化剂催化微晶纤维素转化

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石油化工 2008年第5期37卷 456-459页

作者：马现刚徐恒泳李文钊哈尔滨师范大学化学化工学院黑龙江哈尔滨150025 中国科学院大连化学物理研究所应用催化研究室辽宁大连116023

以γ-Al2.3.载体,采用浸渍法制备了一系列Pt负载量不同的Pt/γ-Al2.3.化剂,研究了Pt/γ-Al2.3.化剂催化微晶纤维素转化的性能及反应机理,考察了反应温度、H2.力、搅拌转速和Pt负载量对微晶纤维素转化率的影响,并对Pt/γ-Al2.3.化... 详细信息

以γ-Al2.3.载体,采用浸渍法制备了一系列Pt负载量不同的Pt/γ-Al2.3.化剂,研究了Pt/γ-Al2.3.化剂催化微晶纤维素转化的性能及反应机理,考察了反应温度、H2.力、搅拌转速和Pt负载量对微晶纤维素转化率的影响,并对Pt/γ-Al2.3.化剂的稳定性进行了测试。实验结果表明,微晶纤维素的转化率随反应温度、H2.力、搅拌转速和Pt负载量的增加而增加,在Pt的质量分数5.0%、温度190℃、H2.力5MPa、搅拌转速550r/min条件下,微晶纤维素转化率可达40.7%;但升高反应温度为反应提供了过高的能量,促进了副反应的发生,反应温度最好控制在2.0℃以下。Pt/γ-Al2.3.化剂的重复使用性较好,经4次回收利用,活性没有明显下降。

关键词：微晶纤维素铀氧化铝催化转化

分子振动光谱量子力学计算中的FD-TD方法

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西南师范大学学报（自然科学版） 2005年第1期30卷 74-78页

作者：黄整钟任斌陈波张世昌西南交通大学理学院四川成都610031 中国工程物理研究院核物理与化学研究所四川绵阳621900

应用时域有限差分法计算了基态N2.X1Σ+g )的振动能级. 介绍了一种基于系统平均能量的截断方法, 在求解双原子分子振动的本征波函数Ψn 和本征振动能量En 的过程中, 采用基于系统平均能量的边界截断较为适宜. 计算得到的光谱项表示为一... 详细信息

应用时域有限差分法计算了基态N2.X1Σ+g )的振动能级. 介绍了一种基于系统平均能量的截断方法, 在求解双原子分子振动的本征波函数Ψn 和本征振动能量En 的过程中, 采用基于系统平均能量的边界截断较为适宜. 计算得到的光谱项表示为一个快速收敛的交错级数, 与文献数据比较, 结果令人满意.

关键词：分子振动量子力学时域有限差分法

Experimental study of nanosecond laser produced plasma dynamics using time-resolved shadowgraph

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Experimental study of nanosecond laser produced plasma dynam...

Fabrication of Transparent Superhydrophilic TiO_2.Nanofilm without UV-irradiation

第十届全国光电技术学术交流会

作者： CHEN Zi-Yu, LI Jun, LI Jian-Feng (institute of Fluid physics, {2. Academy of {3. physics, mianyang 62.900, {2.)

Optical shadowgraph probing of a focused nanosecond laser produced copper plasma plume was investigated experimentally. The temporal evolution of hydrodynamic expansion of the plume was presented and the formation of a shock wave at the plasma-gas interface was also observed. Global plasma profiles from targets with different size were compared and no significant difference found.

关键词： Shadowgraph Laser plasma Shock wave

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稀有金属材料与工程 2012年第S1期41卷 263-267页

作者： Du Wen1,2.Ye Yinping1,Li Hongxuan1,Zhao Fei1,2.Ji Li1,Quan Weilong1,2.Chen Jianmin1,Zhou Huidi1 1 State Key Laboratory of Solid Lubrication,Lanzhou institute of chemical {2.,Chinese Academy of Sciences,Lanzhou 73.000,{3. 2.Graduate University of Chinese Academy of Science,Beijing 100049,{3. Chinese Acad Sci Lanzhou Inst Chem Phys State Key Lab Solid Lubricat Lanzhou 730000 Peoples R China Chinese Acad Sci Grad Univ Beijing 100049 Peoples R China

A transparent phase-pure anatase TiO2.nanofilm was prepared through magnetron sputtering method,and a subsequent annealing treatment awarded it the superhydrophilic *** make clear the mechanism of the heat-induced superhydrophilicity,the chemical composition and surface morphology of the film were investigated in detail and compared before and after the annealing treatment mainly by field emission scanning electron microscopy(FESEM),X-ray diffraction method(XRD),Raman spectroscopy,and X-ray photoelectron spectroscope(XPS).The results suggest that the probable mechanism is in accordance with the UV-induced mechanism,where the heat-induced surface oxygen vacancies and hydroxyl radicals play important roles for achieving the superhydrophilicity.

关键词： superhydrophilicity transparent TiO2 nanofilms magnetron sputtering annealing treatment

金属离子和CTAB胶束催化H_2._2.化对苯二酚的研究

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四川大学学报（自然科学版） 2004年第6期41卷 1241-1245页

作者：韦一良周汉芬覃治中王敏黄忠武汉工业学院生物与化学工程系武汉430023 深圳盐田港供水有限公司广东深圳518000 中国工程物理研究院化工材料研究所四川绵阳621900

作者定性地研究了2.℃,中性(pH6.8)水溶液中金属离子(Cu2.、Fe2.、Fe3.、Co2.和Mn2.)催化H2.2.化对苯二酚,结果表明Fe2.和Fe3.对对苯二酚的氧化有较好的催化效果,Cu2.有一定的催化能力,而Mn2.和Co2.基本没有催化活性,这主要取决于金属... 详细信息

作者定性地研究了2.℃,中性(pH6.8)水溶液中金属离子(Cu2.、Fe2.、Fe3.、Co2.和Mn2.)催化H2.2.化对苯二酚,结果表明Fe2.和Fe3.对对苯二酚的氧化有较好的催化效果,Cu2.有一定的催化能力,而Mn2.和Co2.基本没有催化活性,这主要取决于金属离子与H2.2.合能否产生活性自由基·OH.同时,还定量地研究了CTAB胶束催化H2.2.化对苯二酚.根据"假相模型"获得了CTAB胶束相中对苯二酚氧化反应的速率常数km以及对苯二酚与CTAB胶束的结合常数KS,这表现出了典型的胶束催化特征.

关键词：金属离子胶束催化氧化对苯二酚

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中子Soller准直器的测试与评价

四川大学学报（自然科学版） 2004年第5期41卷 1003-1006页

作者：谢超美张百生徐家云薛艳杰陈东风刘丽鹃陈喜平四川大学物理科学与技术学院成都610064 中国原子能科学研究院核物理研究所北京102413 中国工程物理研究院核物理与化学研究所四川绵阳621900

作者利用中国原子能科学研究院反应堆上的中子谱仪装置,对国内研制的Soller中子准直器进行了测量,并利用MCNP4B程序模拟和解析公式计算,将所得结果进行了分析和比较,对所测准直器的主要技术指标作了评价,证明了国产准直器的性能满足使... 详细信息

作者利用中国原子能科学研究院反应堆上的中子谱仪装置,对国内研制的Soller中子准直器进行了测量,并利用MCNP4B程序模拟和解析公式计算,将所得结果进行了分析和比较,对所测准直器的主要技术指标作了评价,证明了国产准直器的性能满足使用要求.

关键词：准直器评价谱仪中子解析公式中国原子能科学研究院主要技术

Organic thin-film solar cells:Devices and materials

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Science china Chemistry 2012年第4期55卷 553-578页

作者： LI ZhiGang1, ZHAO XinYan2. LI Xin1,2. GAO ZhiQiang1, MI BaoXiu1 & HUANG Wei2.1Jiangsu engineering Centre for Flat-Panel Displays & Solid-state Lighting and School of Materials Science & engineering, Nanjing University of Posts & Telecommunications, Nanjing 2.0046, {2. 2.ey Laboratory for Organic Electronics & Information Displays (KLOEID) {3. of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, Nanjing 2.0046, {2. Jiangsu engineering Centre for Flat-Panel Displays & Solid-state Lighting and School of Materials Science & engineering Nanjing University of Posts & Telecommunications Nanjing China Key Laboratory for Organic Electronics & Information Displays (KLOEID) institute of Advanced Materials (IAM) Nanjing University of Posts & Telecommunications Nanjing China

In recent years, the performance of organic thinfilm solar cells has gained rapid progress, of which the power conversion efficiencies （r/p） of 3.-5% are commonly achieved, which were difficult to obtain years ago and are improving steadily now. The r/p of 7.4% was achieved in the year 2.10, and r/p of 9.2. was disclosed and confirmed at website of Mitsubishi chemical in April, 2.11. The promising future is that the r/p of 10% is achievable according to simulation results. Apparently, these are attributed to material innovations, new device structures, and also the better understanding of device {2.. This article summarizes recent progress in organic thinfilm solar cells related to materials, device structures and working principles. In the device functioning part, after each brief summary of the working principle, the methods for improvements, such as absorption increment, organic/electrode interface engineering, morphological issues, are addressed and summarized accordingly. In addition, for the purpose of increasing exciton diffusion efficiency, the benefit from triplet exciton, which has been proposed in recent years, is highlighted. In the active material parts, the chemical nature of materials and its impact on device performance are discussed. Particularly, emphasis is given toward the insight for better understanding device {2. as well as improvements in device performance either by development of new materials or by new device architecture.

关键词： organic – solar cell – photovoltaic – devices – materials

Dimerization of Propylene by Nickel (Ⅱ) and Cobalt (Ⅱ) Catalysts Based on Bidentate Nitrogen-phosphino Chelating Ligands

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Chinese chemical Letters 2003年第9期14卷 958-961页

作者： Si Zhong WU, Shi Wei LUNational engineering Research Center for Catalysis, Dalian institute of {2. {3., Chinese Academy of Sciences Dalian Chem-Phy {2. Company Ltd.,Dalian 116011 Natl. Eng. Res. Center for Catalysis Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian 116011 China

The catalytic property of propylene dimerization by several nickel (Ⅱ), cobalt (Ⅱ) complexes containing N-P bidentate ligands was studied in combination with organoaluminum co-catalysts. The effects of the type of aluminum co-catalysts and its relative amount, the nature of precursors in terms of ligand backbone and metal center were investigated. The results indicated that precursor I (N,N-dimethyl-2.(diphenylphosphino)aniline nickel (Ⅱ) dichloride) exhibited high activity in propylene dimerization in the presence of the strong Lewis acid Et3.l2.l3. whereas low productivity by its cobalt analogues was observed under identical reaction conditions.

关键词： Bidentate nitrogen-phosphino ligands nickel (Ⅱ) and cobalt (Ⅱ) complexes propylene dimerization Al co-catalyst.